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The map label for this gene is alkJ [H]

Identifier: 120609008

GI number: 120609008

Start: 337555

End: 339249

Strand: Reverse

Name: alkJ [H]

Synonym: Aave_0305

Alternate gene names: 120609008

Gene position: 339249-337555 (Counterclockwise)

Preceding gene: 120609013

Following gene: 120609007

Centisome position: 6.34

GC content: 70.8

Gene sequence:

>1695_bases
ATGAGCGACACCTCTTTCGACTTCATCGTCATCGGCGGCGGTACGGCCGGCGCCCTGCTGTGCAACCGGCTGAGCGCCGA
TCCGCGGCACCGCGTGCTGCTCGTGGAAGCCGGCCGCAAGGACGATTACCACTGGATCCACATTCCCGTGGGCTACCTCT
ACTGCATCGGCAACCCGCGCACCGACTGGCTCTACCAGACCGAACCGGACGAGGGGCTGAACGGCCGCAGCCTGCGCTAC
CCGCGCGGCAAGACCCTGGGCGGCTGCAGCAGCATCAACGGCATGATCTACATGCGCGGGCAGGCACGGGACTACGAGCA
GTGGGCGGAGATCACCGGCGACGATACATGGCGCTGGGAATCCGTGCTGCCGGCCTTCCGCCGCCACGAGGACCACTGGC
GCCTGGACCGCCCCGACACCGCGAGCGAGGCATTCAGGCAGCTGCACGGCAGCAAGACCACCGGCGGCAGCGGTGAATGG
CGGGTGGAGCGCCAGCGCCTGCGCTGGGACGTGCTGGACGCTTTCGCGCGCGCCGCGCAGGAGGCCGGCATTCCGGCCAC
CGACGACTTCAACGGCGGCACCAACGAGGGCGTGGGCTATTTCGAGGTCAACCAGAAGAACGGCTGGCGCTGGAACACCG
CCAAGGCCTTCCTGCGGCCCACCTGCTACGGCCGGCCCAATTTCGAGATGTGGACCGGCGCACAGGCCGCGCGGCTTCTG
CTGGAGTGCCTGCCGGACGGCACCAGCCGCTGCACGGGCGTGGAGGTGTGGAACGGCAGCGAGATGGTCACCGCGCATGC
CGCCCGCGACGTGATCCTGAGCGCGGGTGCCGTCAATTCGCCGCAATTGCTGCAGCTCTCGGGCATCGGCCCGGGAGAGT
TGCTGCAGTCGCACGGCATCCCCGTGGCGCACGACCTGCCGGGGGTGGGCGGCAACCTGCAGGACCACCTGCAGATCCGC
GCGGTCTTCAAGGTGAACGGGGTGCCCACGCTCAACACGCTGGCGGGCAACCTGTGGGGCCGCGCGCGCATCGGGCTCGA
GTACGCCCTGAAGCGCACCGGCCCGATGAGCATGGCGCCGTCGCAACTGGGGGCCTTCACCCGCAGCGCGCCCGGGCAGC
CGCACCCCAATATCCAGTACCACGTGCAGCCCCTGTCGCTCGATGCGTTCGGGGAGCCGCTGCACACGTTCCCGGCGTTC
ACCGCCAGCGTGTGCAACCTCAATCCCACGAGCCGCGGCACGGTGCGCATCCGCAGCCCCCGCTTCCAGGACGCGCCGGC
GATCGCGCCGCGCTACCTGAGCACGCCGGAGGACCGGCAGGTGGCCGCCGATTCGCTGCGTGTCACGCGCCGCATCGTGG
CGCAGCCGGCACTGGCGCCCTACCGGCCCGAGGAATGGAAGCCGGGCCCGCAGTACCAGACCGACGAGGAGCTCGCGCGC
CTGGCGGGTGACATCGCCACCACCATCTTCCACCCCGTGGGCACCACGCGCATGGGCGCCGACGGGGATCCCGCCGCGGT
GCTCGACGCGCGGCTGCGCGTGCGGGACGGCCGGGGCGGCACGATCGCGGGCCTGCGCGTGGTGGATGCGGGCGCCATGC
CCACCATCACCAGCGGCAACACCAATTCGCCCACCCTGATGATCGCGGAAAAGGCGGCCGAATGGCTGCGCGACGAAGCC
CGGGCCGGTGGCTGA

Upstream 100 bases:

>100_bases
GATGGGTGTTTCCCTATCGACAGCCGGGCCGCGCCGCGCACAATGGGCCGGGCCTGCCGCCGGTGCGGCCCCGGAGCGCG
CGACGCCTGCCCCTTCTTCC

Downstream 100 bases:

>100_bases
CGCGCAACCGTACGCGGGCAGATGCACCACCACGGCGCCCGTCCGGCCACCCGGCGCACCGCCATCGCGCACGGGCCGCC
TGCGGGAAACCCCCAATGCA

Product: glucose-methanol-choline oxidoreductase

Products: betaine aldehyde; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MSDTSFDFIVIGGGTAGALLCNRLSADPRHRVLLVEAGRKDDYHWIHIPVGYLYCIGNPRTDWLYQTEPDEGLNGRSLRY
PRGKTLGGCSSINGMIYMRGQARDYEQWAEITGDDTWRWESVLPAFRRHEDHWRLDRPDTASEAFRQLHGSKTTGGSGEW
RVERQRLRWDVLDAFARAAQEAGIPATDDFNGGTNEGVGYFEVNQKNGWRWNTAKAFLRPTCYGRPNFEMWTGAQAARLL
LECLPDGTSRCTGVEVWNGSEMVTAHAARDVILSAGAVNSPQLLQLSGIGPGELLQSHGIPVAHDLPGVGGNLQDHLQIR
AVFKVNGVPTLNTLAGNLWGRARIGLEYALKRTGPMSMAPSQLGAFTRSAPGQPHPNIQYHVQPLSLDAFGEPLHTFPAF
TASVCNLNPTSRGTVRIRSPRFQDAPAIAPRYLSTPEDRQVAADSLRVTRRIVAQPALAPYRPEEWKPGPQYQTDEELAR
LAGDIATTIFHPVGTTRMGADGDPAAVLDARLRVRDGRGGTIAGLRVVDAGAMPTITSGNTNSPTLMIAEKAAEWLRDEA
RAGG

Sequences:

>Translated_564_residues
MSDTSFDFIVIGGGTAGALLCNRLSADPRHRVLLVEAGRKDDYHWIHIPVGYLYCIGNPRTDWLYQTEPDEGLNGRSLRY
PRGKTLGGCSSINGMIYMRGQARDYEQWAEITGDDTWRWESVLPAFRRHEDHWRLDRPDTASEAFRQLHGSKTTGGSGEW
RVERQRLRWDVLDAFARAAQEAGIPATDDFNGGTNEGVGYFEVNQKNGWRWNTAKAFLRPTCYGRPNFEMWTGAQAARLL
LECLPDGTSRCTGVEVWNGSEMVTAHAARDVILSAGAVNSPQLLQLSGIGPGELLQSHGIPVAHDLPGVGGNLQDHLQIR
AVFKVNGVPTLNTLAGNLWGRARIGLEYALKRTGPMSMAPSQLGAFTRSAPGQPHPNIQYHVQPLSLDAFGEPLHTFPAF
TASVCNLNPTSRGTVRIRSPRFQDAPAIAPRYLSTPEDRQVAADSLRVTRRIVAQPALAPYRPEEWKPGPQYQTDEELAR
LAGDIATTIFHPVGTTRMGADGDPAAVLDARLRVRDGRGGTIAGLRVVDAGAMPTITSGNTNSPTLMIAEKAAEWLRDEA
RAGG
>Mature_563_residues
SDTSFDFIVIGGGTAGALLCNRLSADPRHRVLLVEAGRKDDYHWIHIPVGYLYCIGNPRTDWLYQTEPDEGLNGRSLRYP
RGKTLGGCSSINGMIYMRGQARDYEQWAEITGDDTWRWESVLPAFRRHEDHWRLDRPDTASEAFRQLHGSKTTGGSGEWR
VERQRLRWDVLDAFARAAQEAGIPATDDFNGGTNEGVGYFEVNQKNGWRWNTAKAFLRPTCYGRPNFEMWTGAQAARLLL
ECLPDGTSRCTGVEVWNGSEMVTAHAARDVILSAGAVNSPQLLQLSGIGPGELLQSHGIPVAHDLPGVGGNLQDHLQIRA
VFKVNGVPTLNTLAGNLWGRARIGLEYALKRTGPMSMAPSQLGAFTRSAPGQPHPNIQYHVQPLSLDAFGEPLHTFPAFT
ASVCNLNPTSRGTVRIRSPRFQDAPAIAPRYLSTPEDRQVAADSLRVTRRIVAQPALAPYRPEEWKPGPQYQTDEELARL
AGDIATTIFHPVGTTRMGADGDPAAVLDARLRVRDGRGGTIAGLRVVDAGAMPTITSGNTNSPTLMIAEKAAEWLRDEAR
AGG

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=565, Percent_Identity=40, Blast_Score=371, Evalue=1e-103,
Organism=Escherichia coli, GI1786503, Length=559, Percent_Identity=37.567084078712, Blast_Score=320, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17532301, Length=570, Percent_Identity=32.9824561403509, Blast_Score=307, Evalue=9e-84,
Organism=Drosophila melanogaster, GI24642055, Length=596, Percent_Identity=35.4026845637584, Blast_Score=289, Evalue=3e-78,
Organism=Drosophila melanogaster, GI45549471, Length=590, Percent_Identity=34.0677966101695, Blast_Score=281, Evalue=8e-76,
Organism=Drosophila melanogaster, GI45551458, Length=590, Percent_Identity=34.0677966101695, Blast_Score=281, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24642042, Length=587, Percent_Identity=33.0494037478705, Blast_Score=279, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24642048, Length=592, Percent_Identity=32.4324324324324, Blast_Score=271, Evalue=7e-73,
Organism=Drosophila melanogaster, GI18859995, Length=589, Percent_Identity=33.616298811545, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24642059, Length=589, Percent_Identity=31.5789473684211, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI17137792, Length=584, Percent_Identity=33.2191780821918, Blast_Score=239, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24642039, Length=598, Percent_Identity=32.2742474916388, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24650267, Length=589, Percent_Identity=30.7300509337861, Blast_Score=236, Evalue=4e-62,
Organism=Drosophila melanogaster, GI18859993, Length=604, Percent_Identity=31.2913907284768, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24642037, Length=594, Percent_Identity=32.1548821548822, Blast_Score=229, Evalue=5e-60,
Organism=Drosophila melanogaster, GI24642051, Length=592, Percent_Identity=28.5472972972973, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24642035, Length=319, Percent_Identity=39.4984326018809, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24645930, Length=603, Percent_Identity=26.6998341625207, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24642057, Length=612, Percent_Identity=29.2483660130719, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1

Molecular weight: Translated: 61774; Mature: 61643

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTSFDFIVIGGGTAGALLCNRLSADPRHRVLLVEAGRKDDYHWIHIPVGYLYCIGNPR
CCCCCEEEEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEEEEEEECCCCC
TDWLYQTEPDEGLNGRSLRYPRGKTLGGCSSINGMIYMRGQARDYEQWAEITGDDTWRWE
CCCEEECCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCHH
SVLPAFRRHEDHWRLDRPDTASEAFRQLHGSKTTGGSGEWRVERQRLRWDVLDAFARAAQ
HHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
EAGIPATDDFNGGTNEGVGYFEVNQKNGWRWNTAKAFLRPTCYGRPNFEMWTGAQAARLL
HCCCCCCCCCCCCCCCCEEEEEECCCCCCEECCHHHHHCCCCCCCCCCCEECCHHHHHHH
LECLPDGTSRCTGVEVWNGSEMVTAHAARDVILSAGAVNSPQLLQLSGIGPGELLQSHGI
HHHCCCCCCCCCEEEEECCCCEEEHHHHHHHHEECCCCCCCCEEEEECCCHHHHHHHCCC
PVAHDLPGVGGNLQDHLQIRAVFKVNGVPTLNTLAGNLWGRARIGLEYALKRTGPMSMAP
CCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCCH
SQLGAFTRSAPGQPHPNIQYHVQPLSLDAFGEPLHTFPAFTASVCNLNPTSRGTVRIRSP
HHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECC
RFQDAPAIAPRYLSTPEDRQVAADSLRVTRRIVAQPALAPYRPEEWKPGPQYQTDEELAR
CCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
LAGDIATTIFHPVGTTRMGADGDPAAVLDARLRVRDGRGGTIAGLRVVDAGAMPTITSGN
HHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEEEEECCCCCEEEEEEEEECCCCCEEECCC
TNSPTLMIAEKAAEWLRDEARAGG
CCCCEEEEEHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDTSFDFIVIGGGTAGALLCNRLSADPRHRVLLVEAGRKDDYHWIHIPVGYLYCIGNPR
CCCCEEEEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEEEEEEECCCCC
TDWLYQTEPDEGLNGRSLRYPRGKTLGGCSSINGMIYMRGQARDYEQWAEITGDDTWRWE
CCCEEECCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCHH
SVLPAFRRHEDHWRLDRPDTASEAFRQLHGSKTTGGSGEWRVERQRLRWDVLDAFARAAQ
HHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
EAGIPATDDFNGGTNEGVGYFEVNQKNGWRWNTAKAFLRPTCYGRPNFEMWTGAQAARLL
HCCCCCCCCCCCCCCCCEEEEEECCCCCCEECCHHHHHCCCCCCCCCCCEECCHHHHHHH
LECLPDGTSRCTGVEVWNGSEMVTAHAARDVILSAGAVNSPQLLQLSGIGPGELLQSHGI
HHHCCCCCCCCCEEEEECCCCEEEHHHHHHHHEECCCCCCCCEEEEECCCHHHHHHHCCC
PVAHDLPGVGGNLQDHLQIRAVFKVNGVPTLNTLAGNLWGRARIGLEYALKRTGPMSMAP
CCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCCH
SQLGAFTRSAPGQPHPNIQYHVQPLSLDAFGEPLHTFPAFTASVCNLNPTSRGTVRIRSP
HHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECC
RFQDAPAIAPRYLSTPEDRQVAADSLRVTRRIVAQPALAPYRPEEWKPGPQYQTDEELAR
CCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
LAGDIATTIFHPVGTTRMGADGDPAAVLDARLRVRDGRGGTIAGLRVVDAGAMPTITSGN
HHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEEEEECCCCCEEEEEEEEECCCCCEEECCC
TNSPTLMIAEKAAEWLRDEARAGG
CCCCEEEEEHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: choline; acceptor

Specific reaction: choline + acceptor = betaine aldehyde + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]