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The map label for this gene is rhtB [H]

Identifier: 120599160

GI number: 120599160

Start: 2717415

End: 2718038

Strand: Direct

Name: rhtB [H]

Synonym: Sputw3181_2356

Alternate gene names: 120599160

Gene position: 2717415-2718038 (Clockwise)

Preceding gene: 120599159

Following gene: 120599162

Centisome position: 57.71

GC content: 49.68

Gene sequence:

>624_bases
ATGCCTGATTTTGCCTTACTCGCTGTGTTTATCCCGACTTTTTTCTTTGTTTCTATCACGCCGGGCATGTGCATGACACT
CGCCATGACGCTAGGTATGAGCATAGGTGTGCGCCGCACGTTATGGATGATGTTAGGTGAACTCATTGGCGTAGCATTAG
TCGCCACCGCCGCCGTGATGGGGGTTGCCAGTATCATGCTCAACTACCCCGAAGTATTTCAAATCTTTAAGTGGGTTGGC
GGCATTTATTTAGGCTATATCGGCGTACAAATGTGGCGCGCCCGCGGTAAGATGGCCATATTAAGCGATGATGACACCAA
CCCCAGAGCCAGCAGTTTTACCTTGATCTCCCAAGGTTTTATTACCGCTATAGCGAACCCTAAGGGTTGGGCATTTATGG
TGTCGCTACTGCCGCCTTTTATCAATGCCGAGCGCGAAGTTGCACCACAACTCCTCGCCTTGCTGAGCATTATTATGGTG
ACCGAATTTACCTCAATGCTCGCCTATGCCAGCGGCGGTAAGACCCTCCGACTTTTCTTAAGTCGTGGTGATAATATTCG
CTGGTTAAACCGGATTGCAGGCAGCCTAATGATAGGCGTAGGAATTTGGTTAGCCATGAGCTAG

Upstream 100 bases:

>100_bases
AAAATCCATTTGATGAAGCGGTTTAAACAAGCCCATTGGCTATGCTAAGCATTTTCATTACACTATCGGCCTTTTAATGT
TGACTTAAGACTGACCAACG

Downstream 100 bases:

>100_bases
CGGTTTGAACTAACTACTTAAATGTAACTCGCTCGTCCTAAAATGAGCCCAATAAAATCCCGTTAACTCGGTCTTTATGA
AGGCTCTTTTTTGTAGGGCA

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MPDFALLAVFIPTFFFVSITPGMCMTLAMTLGMSIGVRRTLWMMLGELIGVALVATAAVMGVASIMLNYPEVFQIFKWVG
GIYLGYIGVQMWRARGKMAILSDDDTNPRASSFTLISQGFITAIANPKGWAFMVSLLPPFINAEREVAPQLLALLSIIMV
TEFTSMLAYASGGKTLRLFLSRGDNIRWLNRIAGSLMIGVGIWLAMS

Sequences:

>Translated_207_residues
MPDFALLAVFIPTFFFVSITPGMCMTLAMTLGMSIGVRRTLWMMLGELIGVALVATAAVMGVASIMLNYPEVFQIFKWVG
GIYLGYIGVQMWRARGKMAILSDDDTNPRASSFTLISQGFITAIANPKGWAFMVSLLPPFINAEREVAPQLLALLSIIMV
TEFTSMLAYASGGKTLRLFLSRGDNIRWLNRIAGSLMIGVGIWLAMS
>Mature_206_residues
PDFALLAVFIPTFFFVSITPGMCMTLAMTLGMSIGVRRTLWMMLGELIGVALVATAAVMGVASIMLNYPEVFQIFKWVGG
IYLGYIGVQMWRARGKMAILSDDDTNPRASSFTLISQGFITAIANPKGWAFMVSLLPPFINAEREVAPQLLALLSIIMVT
EFTSMLAYASGGKTLRLFLSRGDNIRWLNRIAGSLMIGVGIWLAMS

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=196, Percent_Identity=27.0408163265306, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22647; Mature: 22515

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
7.7 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
7.3 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDFALLAVFIPTFFFVSITPGMCMTLAMTLGMSIGVRRTLWMMLGELIGVALVATAAVM
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVASIMLNYPEVFQIFKWVGGIYLGYIGVQMWRARGKMAILSDDDTNPRASSFTLISQGF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCH
ITAIANPKGWAFMVSLLPPFINAEREVAPQLLALLSIIMVTEFTSMLAYASGGKTLRLFL
HHEECCCCCHHHHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
SRGDNIRWLNRIAGSLMIGVGIWLAMS
CCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDFALLAVFIPTFFFVSITPGMCMTLAMTLGMSIGVRRTLWMMLGELIGVALVATAAVM
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVASIMLNYPEVFQIFKWVGGIYLGYIGVQMWRARGKMAILSDDDTNPRASSFTLISQGF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCH
ITAIANPKGWAFMVSLLPPFINAEREVAPQLLALLSIIMVTEFTSMLAYASGGKTLRLFL
HHEECCCCCHHHHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
SRGDNIRWLNRIAGSLMIGVGIWLAMS
CCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]