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The map label for this gene is lpsJ [H]

Identifier: 120599116

GI number: 120599116

Start: 2666493

End: 2667149

Strand: Direct

Name: lpsJ [H]

Synonym: Sputw3181_2312

Alternate gene names: 120599116

Gene position: 2666493-2667149 (Clockwise)

Preceding gene: 120599115

Following gene: 120599118

Centisome position: 56.63

GC content: 49.92

Gene sequence:

>657_bases
ATGGCATTATCAAGAGAACAACTGGCTCAGCGCGTCGCCAAAGAATTAAAAGACGGCTACTACGTCAACCTCGGTATTGG
TATTCCCACGCTCGTGGCCAACTACATTCCCGAAGGCATGGAAGTCATGCTGCAATCAGAAAACGGCCTACTCGGCATGG
GTGAATTCCCAACCGAAGACACCATAGATCCCGATTTAATCAATGCGGGCAAACAAACCGTTACCGCCGTGAAAGGCGCG
TCGTTTTTCTCTTCAGCCGAGAGCTTTGCCATGATCCGTGGCGGCCATGTGGATTTAACCGTACTCGGCGCCTTTGAAGT
CGATGTGAACGGCTCTATCGCCTCATGGATGATCCCCGGCAAACTCATCAAAGGCATGGGCGGCGCAATGGATTTAGTCG
CGGGCGCCGAAAACATTATCGTGACTATGATGCACGCCGATAAATACGGCAATTCTAAGCTGCTACCCGTGTGCGAATTG
CCGCTAACAGGTTACGGCTGCATTAAACGCGTCGTCACAGATTTAGCCTTTATCGAAATCAAAGATGGCGCTTTCCATCT
GTTAGAACGTGCGCCCGGTGTGAGTGTTGAAGAAATTGTTAGCAAAACCGCTGGCAAATTAATCGTACCAGACCATGTAC
CAGAGATGGTGTTTTAA

Upstream 100 bases:

>100_bases
GTTAACGCAATTGGCACTTAGATCTCTAGAGTAAAAACTAAGGGTAAAAACCTAAGAGTAAAGACCAAAGCGTCAAAGCC
AAATAGAAAGGATAGATATC

Downstream 100 bases:

>100_bases
GCATTCTTTAACGTGCGCAAGAAACCTATAACGATAAGGCTTGAAACTTACGTTAGTTTAAAGCGTTAGCCTAAAAAGTG
TTGAGCGTTAACCGACGTTT

Product: 3-oxoacid CoA-transferase subunit B

Products: NA

Alternate protein names: OXCT B; Succinyl CoA:3-oxoacid CoA-transferase [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MALSREQLAQRVAKELKDGYYVNLGIGIPTLVANYIPEGMEVMLQSENGLLGMGEFPTEDTIDPDLINAGKQTVTAVKGA
SFFSSAESFAMIRGGHVDLTVLGAFEVDVNGSIASWMIPGKLIKGMGGAMDLVAGAENIIVTMMHADKYGNSKLLPVCEL
PLTGYGCIKRVVTDLAFIEIKDGAFHLLERAPGVSVEEIVSKTAGKLIVPDHVPEMVF

Sequences:

>Translated_218_residues
MALSREQLAQRVAKELKDGYYVNLGIGIPTLVANYIPEGMEVMLQSENGLLGMGEFPTEDTIDPDLINAGKQTVTAVKGA
SFFSSAESFAMIRGGHVDLTVLGAFEVDVNGSIASWMIPGKLIKGMGGAMDLVAGAENIIVTMMHADKYGNSKLLPVCEL
PLTGYGCIKRVVTDLAFIEIKDGAFHLLERAPGVSVEEIVSKTAGKLIVPDHVPEMVF
>Mature_217_residues
ALSREQLAQRVAKELKDGYYVNLGIGIPTLVANYIPEGMEVMLQSENGLLGMGEFPTEDTIDPDLINAGKQTVTAVKGAS
FFSSAESFAMIRGGHVDLTVLGAFEVDVNGSIASWMIPGKLIKGMGGAMDLVAGAENIIVTMMHADKYGNSKLLPVCELP
LTGYGCIKRVVTDLAFIEIKDGAFHLLERAPGVSVEEIVSKTAGKLIVPDHVPEMVF

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=213, Percent_Identity=51.1737089201878, Blast_Score=215, Evalue=2e-56,
Organism=Homo sapiens, GI11545841, Length=215, Percent_Identity=48.3720930232558, Blast_Score=211, Evalue=4e-55,
Organism=Escherichia coli, GI1788552, Length=206, Percent_Identity=47.5728155339806, Blast_Score=204, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17531517, Length=216, Percent_Identity=53.2407407407407, Blast_Score=217, Evalue=4e-57,
Organism=Drosophila melanogaster, GI21358325, Length=214, Percent_Identity=56.0747663551402, Blast_Score=247, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24655794, Length=214, Percent_Identity=56.0747663551402, Blast_Score=247, Evalue=4e-66,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 23286; Mature: 23155

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSREQLAQRVAKELKDGYYVNLGIGIPTLVANYIPEGMEVMLQSENGLLGMGEFPTED
CCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCC
TIDPDLINAGKQTVTAVKGASFFSSAESFAMIRGGHVDLTVLGAFEVDVNGSIASWMIPG
CCCHHHHHCCCHHHHHHHCCHHHHHHHHHHEEECCCEEEEEEEEEEEECCCCEEEEECCH
KLIKGMGGAMDLVAGAENIIVTMMHADKYGNSKLLPVCELPLTGYGCIKRVVTDLAFIEI
HHHHCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHEEEEE
KDGAFHLLERAPGVSVEEIVSKTAGKLIVPDHVPEMVF
CCCHHHHHHCCCCCCHHHHHHHHCCCEECCCCCCCCCC
>Mature Secondary Structure 
ALSREQLAQRVAKELKDGYYVNLGIGIPTLVANYIPEGMEVMLQSENGLLGMGEFPTED
CCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCC
TIDPDLINAGKQTVTAVKGASFFSSAESFAMIRGGHVDLTVLGAFEVDVNGSIASWMIPG
CCCHHHHHCCCHHHHHHHCCHHHHHHHHHHEEECCCEEEEEEEEEEEECCCCEEEEECCH
KLIKGMGGAMDLVAGAENIIVTMMHADKYGNSKLLPVCELPLTGYGCIKRVVTDLAFIEI
HHHHCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHEEEEE
KDGAFHLLERAPGVSVEEIVSKTAGKLIVPDHVPEMVF
CCCHHHHHHCCCCCCHHHHHHHHCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9385134 [H]