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The map label for this gene is mvaB [H]

Identifier: 120599114

GI number: 120599114

Start: 2664675

End: 2665607

Strand: Direct

Name: mvaB [H]

Synonym: Sputw3181_2310

Alternate gene names: 120599114

Gene position: 2664675-2665607 (Clockwise)

Preceding gene: 120599113

Following gene: 120599115

Centisome position: 56.59

GC content: 52.63

Gene sequence:

>933_bases
ATGTCTGCGCTGGATCTTGCGAACTTAAGCGCCACTTCGGATAAGGTAAGCTTATTCGAGATGGGTCCCCGTGATGGCTT
GCAGAATGAGGCCGCCGTCCCGACAGAGGCTAAAGTCGCCTTGATTGAAGCCCTTGCCGATGCGGGCGTGAAGCGAATTG
AATCCGGCAGTTTTGTGTCACCCAAATGGGTGCCACAAATGGCCGATAGCGGCGACGTATTGCGCCAAATTCGCCGTCAA
GCGGGCGTGGTTTACAGCGCCCTCACCCCCAATGTCAAAGGCTTAGAGCTAGCGCTGGATGCAAAAGCTTCCGAAGTCGC
TATCTTTGGTGCGGCGTCACAAAGCTTTAGCCAGCGCAATATCAACTGTTCGATTGAAGAGTCGATTGAACGCTTTATCC
CGTTGATGGACATGGCAAAAGCCGCCAATATCCCAGTTCGTGGCTATGTGTCCTGCGTTTTAGGTTGCCCCTATGAAGGA
GAAATTGCGGCGAGCGAAGTGGCAAGAGTGTCTGAAATCGTTTACAAAATGGGCTGTTACGAAATTTCGCTCGGCGACAC
TATCGGTGTAGGCACGCCGTTAAAAGCCCGTAAAATGCTGCAAGCCGTGATGGAGCGAGTCCCGGTTGAAAAGCTCGCCC
TGCACTTCCATGACACCTATGGCCAAGCATTAGCCAATATTACCGCCTGCCTCGACTTGGGCGTGCGCAGCTTTGACGCC
TCGGTGGCGGGCTTAGGTGGTTGCCCGTATGCGAAAGGCGCGTCGGGCAATCTGGCGAGTGAAGACTTGGTCTATATGCT
CCATGGTCTGGGCTTAAACACAGGTATCGATTTAGAAAAACTGGCATTAGCGGGTTTTGGGATCAGCAAACAATTAAATC
GTCTTAATGGCTCTAAAGTCGCCAATGCAATCTTACAGGGCGCCAAAGCCTAG

Upstream 100 bases:

>100_bases
CGGTATTGTTACCGAGTTCTACTTTAAGGCGGGCGAGTTAGTGAGCGATGGCGCCGTGCTGCTCCATGTTGAGCCCAAAG
CCCAGAGCGAGGAAGCCTAA

Downstream 100 bases:

>100_bases
GTTCTAGGTTCTAGAAATGATTGGACACGAATAACAATTTAAAGGACATCAAAATGGCAGGACTCAATAAAGTCGTCAGC
AGCTATGAAGAAGCGTTAAC

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSALDLANLSATSDKVSLFEMGPRDGLQNEAAVPTEAKVALIEALADAGVKRIESGSFVSPKWVPQMADSGDVLRQIRRQ
AGVVYSALTPNVKGLELALDAKASEVAIFGAASQSFSQRNINCSIEESIERFIPLMDMAKAANIPVRGYVSCVLGCPYEG
EIAASEVARVSEIVYKMGCYEISLGDTIGVGTPLKARKMLQAVMERVPVEKLALHFHDTYGQALANITACLDLGVRSFDA
SVAGLGGCPYAKGASGNLASEDLVYMLHGLGLNTGIDLEKLALAGFGISKQLNRLNGSKVANAILQGAKA

Sequences:

>Translated_310_residues
MSALDLANLSATSDKVSLFEMGPRDGLQNEAAVPTEAKVALIEALADAGVKRIESGSFVSPKWVPQMADSGDVLRQIRRQ
AGVVYSALTPNVKGLELALDAKASEVAIFGAASQSFSQRNINCSIEESIERFIPLMDMAKAANIPVRGYVSCVLGCPYEG
EIAASEVARVSEIVYKMGCYEISLGDTIGVGTPLKARKMLQAVMERVPVEKLALHFHDTYGQALANITACLDLGVRSFDA
SVAGLGGCPYAKGASGNLASEDLVYMLHGLGLNTGIDLEKLALAGFGISKQLNRLNGSKVANAILQGAKA
>Mature_309_residues
SALDLANLSATSDKVSLFEMGPRDGLQNEAAVPTEAKVALIEALADAGVKRIESGSFVSPKWVPQMADSGDVLRQIRRQA
GVVYSALTPNVKGLELALDAKASEVAIFGAASQSFSQRNINCSIEESIERFIPLMDMAKAANIPVRGYVSCVLGCPYEGE
IAASEVARVSEIVYKMGCYEISLGDTIGVGTPLKARKMLQAVMERVPVEKLALHFHDTYGQALANITACLDLGVRSFDAS
VAGLGGCPYAKGASGNLASEDLVYMLHGLGLNTGIDLEKLALAGFGISKQLNRLNGSKVANAILQGAKA

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=304, Percent_Identity=58.2236842105263, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI109150422, Length=302, Percent_Identity=55.6291390728477, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI109150427, Length=300, Percent_Identity=55.6666666666667, Blast_Score=349, Evalue=2e-96,
Organism=Homo sapiens, GI260654708, Length=136, Percent_Identity=64.7058823529412, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1786261, Length=249, Percent_Identity=27.3092369477912, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17510563, Length=280, Percent_Identity=52.8571428571429, Blast_Score=304, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24582381, Length=303, Percent_Identity=55.4455445544555, Blast_Score=351, Evalue=3e-97,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 32638; Mature: 32507

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS01062 HMG_COA_LYASE ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALDLANLSATSDKVSLFEMGPRDGLQNEAAVPTEAKVALIEALADAGVKRIESGSFVS
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
PKWVPQMADSGDVLRQIRRQAGVVYSALTPNVKGLELALDAKASEVAIFGAASQSFSQRN
CCCCCCCCCCHHHHHHHHHHHCEEEEECCCCCCCEEEEEECCCCEEEEEECCCHHHHHCC
INCSIEESIERFIPLMDMAKAANIPVRGYVSCVLGCPYEGEIAASEVARVSEIVYKMGCY
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCE
EISLGDTIGVGTPLKARKMLQAVMERVPVEKLALHFHDTYGQALANITACLDLGVRSFDA
EEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SVAGLGGCPYAKGASGNLASEDLVYMLHGLGLNTGIDLEKLALAGFGISKQLNRLNGSKV
CHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHH
ANAILQGAKA
HHHHHHCCCC
>Mature Secondary Structure 
SALDLANLSATSDKVSLFEMGPRDGLQNEAAVPTEAKVALIEALADAGVKRIESGSFVS
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
PKWVPQMADSGDVLRQIRRQAGVVYSALTPNVKGLELALDAKASEVAIFGAASQSFSQRN
CCCCCCCCCCHHHHHHHHHHHCEEEEECCCCCCCEEEEEECCCCEEEEEECCCHHHHHCC
INCSIEESIERFIPLMDMAKAANIPVRGYVSCVLGCPYEGEIAASEVARVSEIVYKMGCY
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCE
EISLGDTIGVGTPLKARKMLQAVMERVPVEKLALHFHDTYGQALANITACLDLGVRSFDA
EEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SVAGLGGCPYAKGASGNLASEDLVYMLHGLGLNTGIDLEKLALAGFGISKQLNRLNGSKV
CHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHH
ANAILQGAKA
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]