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The map label for this gene is mltD [H]

Identifier: 120599054

GI number: 120599054

Start: 2597344

End: 2598888

Strand: Direct

Name: mltD [H]

Synonym: Sputw3181_2250

Alternate gene names: 120599054

Gene position: 2597344-2598888 (Clockwise)

Preceding gene: 120599053

Following gene: 120599058

Centisome position: 55.16

GC content: 42.46

Gene sequence:

>1545_bases
ATGAGAGTATCACTTTATATTGTAGTAGGGGGATTTGCCCTACTCGCGGGTTGTCAGACATTGGATAACCCTGAGGTTAC
TAAGCCAGAAGCTAAAGCCGTCACAAAACCAAATACCGTCACTCCCTTTTATCTCTCATCAGAGGTTGAATCGGCGCAAA
TTACCGATGTGTGGGAACGAATTCGTCAAGGAATGCAATTACCTATCCCTGATCAAAAATTAGTCAATCAATACCGTGAT
TGGTATATCAAGAATCCGAAACATCTCGAAATTATTTCTGAGCGTGCGGCTCCTTATATGTATATGATTGTGGAAGAACT
TGAGAAACGTCATCTTCCGATTGAGATTGCACTACTGCCCATTATCGAAAGTGCCTTCGACCCATCTGCCTCCTCTGCCA
GCGCCGCTTCGGGGTTATGGCAATTTACCACACCTATGGCCAATCATTTTGGATTAGAAATGAACTGGTGGTATGACGGT
CGCCGTGATGTACCTGCCTCGACGATTGCCGCATTGGATATGTTGGAATATCTCTATGATAAAACAAATAATAATTGGCT
CTATGCTTTTGCAGCTTATAATTCTGGGGAATCACGGGTTCTCAATGCAATAAAAAATAATGAGGCAAAAGGGTTAAAAA
CGGATTTTTGGTCACTTAACCTACCTAAAGAAACTAAACGCTATGTGCCTCAGCTTCTCGCGCTTGCAGAGGTTATTAAG
CATGCCGACGAGTATGGTATTACTTTAGCACCAATAGAGAATACCCCTTCAATTGAAGTTGTTGATATTGATAGCCAAAT
TGATCTTGCTATGGCTGCAGGACTTGCCAATATGACAACGATGGAATTACAAAAACTCAACCCTGGCTATCATCGCTGGG
CAACGTCGCCCTTGGGACCACACACCTTAGTATTGCCTATAGATAAATCTGAAGAGTTTAAAAAAGCACTGGCGGAGACA
GAGAGCGAAGCAAGAATGAGCTGGTTGCGATATACCATCAAATCCGGTGACAGTATTGGCGCGATCGCTAAAAAGCACCA
TACAACTGTAGCAGCAATTCGCGCCGCTAATGGTATGAAAAACAATACGATTGTTGCAGGTAAGCACCTGATTATTCCAG
TGTCTGCGGATGATAAACAGTTATACGCTCAATCGACAAGCCAGAAATTACCGAAGAAATTACGCTCTTCAACAAATACT
CAACTGACGCATAAAGTGCAATCCGGCGACACCCTGTGGGAAATTGCAAAAAAGTATAATGTGACAATCAAACAACTGAC
TGTATGGAATCACTTAAAGGAAGATAGCAAACTGCAAACAGGGCAAAAGCTGGCCGTTTTAGCGCCTAAACACACTGAAA
CTACCGAAAAAACCCGTACCGTCAGCTATAAAGTAAAATCCGGTGATTCATTGGCTCGAATAGCTAGCAAGTTTAACGTC
ACCGTCACTGAATTGTTGGAATGGAACAGCTTAACTCAATCGCAATATTTACAACCTGGCCAAGTATTAAAACTGGTTGT
CGATGAAAGCAAATTAAACGCTTAG

Upstream 100 bases:

>100_bases
TACTTTGCGCTCTGTGTTGTCATTATCCTATTAAAAATCATGCTTTTAATCTACAAGCATGGCGCACTGACAATGCACTA
ACAGAGGACTACCGTTTTCC

Downstream 100 bases:

>100_bases
TGACAACGCTATTTAAGACGGGTCAAACCCAAAAGATAAATCAAAAAAGGCAGCGAGTCCTTGAAACGCTGCCTTTTTCA
GTACTACATTCAACACAATC

Product: MltD domain-containing protein

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MRVSLYIVVGGFALLAGCQTLDNPEVTKPEAKAVTKPNTVTPFYLSSEVESAQITDVWERIRQGMQLPIPDQKLVNQYRD
WYIKNPKHLEIISERAAPYMYMIVEELEKRHLPIEIALLPIIESAFDPSASSASAASGLWQFTTPMANHFGLEMNWWYDG
RRDVPASTIAALDMLEYLYDKTNNNWLYAFAAYNSGESRVLNAIKNNEAKGLKTDFWSLNLPKETKRYVPQLLALAEVIK
HADEYGITLAPIENTPSIEVVDIDSQIDLAMAAGLANMTTMELQKLNPGYHRWATSPLGPHTLVLPIDKSEEFKKALAET
ESEARMSWLRYTIKSGDSIGAIAKKHHTTVAAIRAANGMKNNTIVAGKHLIIPVSADDKQLYAQSTSQKLPKKLRSSTNT
QLTHKVQSGDTLWEIAKKYNVTIKQLTVWNHLKEDSKLQTGQKLAVLAPKHTETTEKTRTVSYKVKSGDSLARIASKFNV
TVTELLEWNSLTQSQYLQPGQVLKLVVDESKLNA

Sequences:

>Translated_514_residues
MRVSLYIVVGGFALLAGCQTLDNPEVTKPEAKAVTKPNTVTPFYLSSEVESAQITDVWERIRQGMQLPIPDQKLVNQYRD
WYIKNPKHLEIISERAAPYMYMIVEELEKRHLPIEIALLPIIESAFDPSASSASAASGLWQFTTPMANHFGLEMNWWYDG
RRDVPASTIAALDMLEYLYDKTNNNWLYAFAAYNSGESRVLNAIKNNEAKGLKTDFWSLNLPKETKRYVPQLLALAEVIK
HADEYGITLAPIENTPSIEVVDIDSQIDLAMAAGLANMTTMELQKLNPGYHRWATSPLGPHTLVLPIDKSEEFKKALAET
ESEARMSWLRYTIKSGDSIGAIAKKHHTTVAAIRAANGMKNNTIVAGKHLIIPVSADDKQLYAQSTSQKLPKKLRSSTNT
QLTHKVQSGDTLWEIAKKYNVTIKQLTVWNHLKEDSKLQTGQKLAVLAPKHTETTEKTRTVSYKVKSGDSLARIASKFNV
TVTELLEWNSLTQSQYLQPGQVLKLVVDESKLNA
>Mature_514_residues
MRVSLYIVVGGFALLAGCQTLDNPEVTKPEAKAVTKPNTVTPFYLSSEVESAQITDVWERIRQGMQLPIPDQKLVNQYRD
WYIKNPKHLEIISERAAPYMYMIVEELEKRHLPIEIALLPIIESAFDPSASSASAASGLWQFTTPMANHFGLEMNWWYDG
RRDVPASTIAALDMLEYLYDKTNNNWLYAFAAYNSGESRVLNAIKNNEAKGLKTDFWSLNLPKETKRYVPQLLALAEVIK
HADEYGITLAPIENTPSIEVVDIDSQIDLAMAAGLANMTTMELQKLNPGYHRWATSPLGPHTLVLPIDKSEEFKKALAET
ESEARMSWLRYTIKSGDSIGAIAKKHHTTVAAIRAANGMKNNTIVAGKHLIIPVSADDKQLYAQSTSQKLPKKLRSSTNT
QLTHKVQSGDTLWEIAKKYNVTIKQLTVWNHLKEDSKLQTGQKLAVLAPKHTETTEKTRTVSYKVKSGDSLARIASKFNV
TVTELLEWNSLTQSQYLQPGQVLKLVVDESKLNA

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=448, Percent_Identity=33.0357142857143, Blast_Score=270, Evalue=1e-73,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 57584; Mature: 57584

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSLYIVVGGFALLAGCQTLDNPEVTKPEAKAVTKPNTVTPFYLSSEVESAQITDVWER
CEEEEEEEEHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCEEECCCCCCHHHHHHHHH
IRQGMQLPIPDQKLVNQYRDWYIKNPKHLEIISERAAPYMYMIVEELEKRHLPIEIALLP
HHCCCCCCCCCHHHHHHHHHCEECCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEH
IIESAFDPSASSASAASGLWQFTTPMANHFGLEMNWWYDGRRDVPASTIAALDMLEYLYD
HHHHHCCCCCCCCHHHCCCEEECCCHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
KTNNNWLYAFAAYNSGESRVLNAIKNNEAKGLKTDFWSLNLPKETKRYVPQLLALAEVIK
CCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
HADEYGITLAPIENTPSIEVVDIDSQIDLAMAAGLANMTTMELQKLNPGYHRWATSPLGP
HHHHCCEEEEECCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCHHHCCCCCCCC
HTLVLPIDKSEEFKKALAETESEARMSWLRYTIKSGDSIGAIAKKHHTTVAAIRAANGMK
CEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHEEEHHHCCCC
NNTIVAGKHLIIPVSADDKQLYAQSTSQKLPKKLRSSTNTQLTHKVQSGDTLWEIAKKYN
CCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHC
VTIKQLTVWNHLKEDSKLQTGQKLAVLAPKHTETTEKTRTVSYKVKSGDSLARIASKFNV
CEEEEEEHHHHHCCCCHHCCCCEEEEECCCCCCCCCCEEEEEEEECCCCHHHHHHHHCCC
TVTELLEWNSLTQSQYLQPGQVLKLVVDESKLNA
CHHHHHHHCCCCHHHCCCCCCEEEEEECHHHCCC
>Mature Secondary Structure
MRVSLYIVVGGFALLAGCQTLDNPEVTKPEAKAVTKPNTVTPFYLSSEVESAQITDVWER
CEEEEEEEEHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCEEECCCCCCHHHHHHHHH
IRQGMQLPIPDQKLVNQYRDWYIKNPKHLEIISERAAPYMYMIVEELEKRHLPIEIALLP
HHCCCCCCCCCHHHHHHHHHCEECCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEH
IIESAFDPSASSASAASGLWQFTTPMANHFGLEMNWWYDGRRDVPASTIAALDMLEYLYD
HHHHHCCCCCCCCHHHCCCEEECCCHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
KTNNNWLYAFAAYNSGESRVLNAIKNNEAKGLKTDFWSLNLPKETKRYVPQLLALAEVIK
CCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
HADEYGITLAPIENTPSIEVVDIDSQIDLAMAAGLANMTTMELQKLNPGYHRWATSPLGP
HHHHCCEEEEECCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCHHHCCCCCCCC
HTLVLPIDKSEEFKKALAETESEARMSWLRYTIKSGDSIGAIAKKHHTTVAAIRAANGMK
CEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHEEEHHHCCCC
NNTIVAGKHLIIPVSADDKQLYAQSTSQKLPKKLRSSTNTQLTHKVQSGDTLWEIAKKYN
CCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHC
VTIKQLTVWNHLKEDSKLQTGQKLAVLAPKHTETTEKTRTVSYKVKSGDSLARIASKFNV
CEEEEEEHHHHHCCCCHHCCCCEEEEECCCCCCCCCCEEEEEEEECCCCHHHHHHHHCCC
TVTELLEWNSLTQSQYLQPGQVLKLVVDESKLNA
CHHHHHHHCCCCHHHCCCCCCEEEEEECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]