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The map label for this gene is gloB [H]

Identifier: 120599053

GI number: 120599053

Start: 2596298

End: 2597101

Strand: Direct

Name: gloB [H]

Synonym: Sputw3181_2249

Alternate gene names: 120599053

Gene position: 2596298-2597101 (Clockwise)

Preceding gene: 120599050

Following gene: 120599054

Centisome position: 55.14

GC content: 42.79

Gene sequence:

>804_bases
ATGCTCACTATAACAGCGATTAAAGCCTTTAATGACAATTATATCTGGGTGTTACAACAAACTCCCCACTCGCAGGTTTA
TGTTGTCGATCCTGGTGATGCTAAGGTCGTTATTGCCTATCTGGTCTCCCATCAGTTAGCACTCGCTGGTATTTTGCTCA
CGCACCATCACCAAGATCATACTGGCGGTGTCGGGGAACTTAAACGTTACGTTGAGCAAACTTCATCACAAACACTGCAG
GTTTACGGACCACAAAAAGAAAATATTGCTCATGTCAATGTACCACTTAATCCTGAAATGCGAACTGAACTCACCCTTCC
TTTCCTTAACGCTCAAATACAAGTGCTGAACGTGCCAGGCCATACAGCTGGACATATTGCCTATGTCATTGAGGATGCGC
TTTTTTGTGGTGATACTTTATTCAGTGCAGGCTGTGGTCGACTATTTGAAGGCACCGCGGAGCAAATGCTTAGCTCTCTT
ACACTATTAGCCAAACTTCCTGCGAATACTCGAGTTTTCTGCGCCCATGAATATACCCTCTCCAATCTTAAATTTGCTCT
AACAGTCGAGCCTAACAATCTAAATTTACAGGCATATATGCAAAAAGCCACAGAAATGCGTGCTCAAAATAGCGCTACAA
TTCCCTCAAGTATTGCCTTGGAGCGAGCTATCAATCCTTTTTTGCGCGTGGGAGAACAAAGCATTATTGATAGTATAAAA
CAGCATTTTGATGATCCCGATCCTATCAAACTAGATGAACTCACTTGTTTTACCCGCCTTCGGCAGTGGAAAGATATTTT
TTAG

Upstream 100 bases:

>100_bases
GTACAGAACACTATGCCATTATGGCAAAAGTCGCCAAGGTGTATCGACTTGTGGATAATAACCTTATCTATAAATTGAAA
TACAGCCATAAGGTGGTTCT

Downstream 100 bases:

>100_bases
CACCAACCGATCGTTTTTAGATTGAAATTTATACAGTTTTTTCCACTTTTGATGATGTAAGTCAAAAATAGCCTTACAAG
ATGTACGATAATTAAGGGAC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MLTITAIKAFNDNYIWVLQQTPHSQVYVVDPGDAKVVIAYLVSHQLALAGILLTHHHQDHTGGVGELKRYVEQTSSQTLQ
VYGPQKENIAHVNVPLNPEMRTELTLPFLNAQIQVLNVPGHTAGHIAYVIEDALFCGDTLFSAGCGRLFEGTAEQMLSSL
TLLAKLPANTRVFCAHEYTLSNLKFALTVEPNNLNLQAYMQKATEMRAQNSATIPSSIALERAINPFLRVGEQSIIDSIK
QHFDDPDPIKLDELTCFTRLRQWKDIF

Sequences:

>Translated_267_residues
MLTITAIKAFNDNYIWVLQQTPHSQVYVVDPGDAKVVIAYLVSHQLALAGILLTHHHQDHTGGVGELKRYVEQTSSQTLQ
VYGPQKENIAHVNVPLNPEMRTELTLPFLNAQIQVLNVPGHTAGHIAYVIEDALFCGDTLFSAGCGRLFEGTAEQMLSSL
TLLAKLPANTRVFCAHEYTLSNLKFALTVEPNNLNLQAYMQKATEMRAQNSATIPSSIALERAINPFLRVGEQSIIDSIK
QHFDDPDPIKLDELTCFTRLRQWKDIF
>Mature_267_residues
MLTITAIKAFNDNYIWVLQQTPHSQVYVVDPGDAKVVIAYLVSHQLALAGILLTHHHQDHTGGVGELKRYVEQTSSQTLQ
VYGPQKENIAHVNVPLNPEMRTELTLPFLNAQIQVLNVPGHTAGHIAYVIEDALFCGDTLFSAGCGRLFEGTAEQMLSSL
TLLAKLPANTRVFCAHEYTLSNLKFALTVEPNNLNLQAYMQKATEMRAQNSATIPSSIALERAINPFLRVGEQSIIDSIK
QHFDDPDPIKLDELTCFTRLRQWKDIF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538322, Length=274, Percent_Identity=34.6715328467153, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI94538320, Length=274, Percent_Identity=34.6715328467153, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI116642887, Length=275, Percent_Identity=33.8181818181818, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI21703352, Length=236, Percent_Identity=36.4406779661017, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI14150041, Length=241, Percent_Identity=36.0995850622407, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI46361987, Length=209, Percent_Identity=35.8851674641148, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI1786406, Length=242, Percent_Identity=45.0413223140496, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1787158, Length=205, Percent_Identity=29.7560975609756, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=282, Percent_Identity=31.9148936170213, Blast_Score=134, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6320478, Length=238, Percent_Identity=28.9915966386555, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324614, Length=240, Percent_Identity=27.0833333333333, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21356335, Length=274, Percent_Identity=38.3211678832117, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24667711, Length=274, Percent_Identity=38.3211678832117, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24667703, Length=274, Percent_Identity=38.3211678832117, Blast_Score=177, Evalue=6e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 29794; Mature: 29794

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTITAIKAFNDNYIWVLQQTPHSQVYVVDPGDAKVVIAYLVSHQLALAGILLTHHHQDH
CEEEEEEEEECCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
TGGVGELKRYVEQTSSQTLQVYGPQKENIAHVNVPLNPEMRTELTLPFLNAQIQVLNVPG
CCCHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCCEEEEEEEECCEEEEEECCC
HTAGHIAYVIEDALFCGDTLFSAGCGRLFEGTAEQMLSSLTLLAKLPANTRVFCAHEYTL
CCCCHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEE
SNLKFALTVEPNNLNLQAYMQKATEMRAQNSATIPSSIALERAINPFLRVGEQSIIDSIK
CCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
QHFDDPDPIKLDELTCFTRLRQWKDIF
HHCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTITAIKAFNDNYIWVLQQTPHSQVYVVDPGDAKVVIAYLVSHQLALAGILLTHHHQDH
CEEEEEEEEECCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
TGGVGELKRYVEQTSSQTLQVYGPQKENIAHVNVPLNPEMRTELTLPFLNAQIQVLNVPG
CCCHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCCEEEEEEEECCEEEEEECCC
HTAGHIAYVIEDALFCGDTLFSAGCGRLFEGTAEQMLSSLTLLAKLPANTRVFCAHEYTL
CCCCHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEE
SNLKFALTVEPNNLNLQAYMQKATEMRAQNSATIPSSIALERAINPFLRVGEQSIIDSIK
CCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
QHFDDPDPIKLDELTCFTRLRQWKDIF
HHCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA