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The map label for this gene is nth [H]

Identifier: 120598973

GI number: 120598973

Start: 2495393

End: 2496034

Strand: Direct

Name: nth [H]

Synonym: Sputw3181_2165

Alternate gene names: 120598973

Gene position: 2495393-2496034 (Clockwise)

Preceding gene: 120598972

Following gene: 120598976

Centisome position: 53.0

GC content: 45.48

Gene sequence:

>642_bases
ATGAATCAAGCAAAACGTATCCAGATCCTCACTCGTCTTCGTGATAACAATCCGAAACCCGAAACTGAGCTCAACTTTAC
TAGTCCTTTTGAGTTATTAGTCGCGGTAACCCTCTCAGCACAAGCGACCGATGTGAGCGTGAATAAGGCGACAGATAAAC
TGTTTCCTGTCGCCAATACTGCCCACAGCATTTATGCCCTAGGGGTTGAGGGATTAAAGGAATATATCAAGACCATTGGC
CTTTATAACAACAAAGCCGTTAATGTGATCAAAGCCTGCGAGATCTTAATCGAGAAGTACAATGGTGAAGTGCCAGAAGA
TAGAGAAGCCCTTGAAGCACTACCTGGTGTGGGCCGAAAAACCGCGAACGTGGTGCTCAATACCGCCTTTGGCTGGCCGA
CCATTGCCGTAGATACTCATATTTTTCGTCTTGCCAATCGCACTAAATTTGCCCCCGGTAAAAACGTGGTCGAAGTAGAA
GAACGGATGCTAAAAGTTGTGCCGGATGAGTTTAAAGTGGATGTACATCATTGGTTTATTCTGCACGGCCGCTACACATG
CTTAGCACGAAAGCCACGCTGTGGCAGTTGCATTATTGAGGATTTGTGTGAGTTTAAAGAGAAGGTTTATCCCGAAGAAT
AA

Upstream 100 bases:

>100_bases
AATTGATAAAAAACTCAAAGAACACCAACCACAAGTCGCCACCGAGCCAAGTGTGACACGCGCACGGATCACTAAGGTCA
GTTAACGAAGAATAGTGAAT

Downstream 100 bases:

>100_bases
ACCTATTGAACAGACAATCCCATTACACATGGAATTGTCTGTTTAAAATGCTAAATCAGCAACGCTTCCGGTATTGGCCA
CTTTGCCACGAGCCACTCCC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MNQAKRIQILTRLRDNNPKPETELNFTSPFELLVAVTLSAQATDVSVNKATDKLFPVANTAHSIYALGVEGLKEYIKTIG
LYNNKAVNVIKACEILIEKYNGEVPEDREALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRLANRTKFAPGKNVVEVE
ERMLKVVPDEFKVDVHHWFILHGRYTCLARKPRCGSCIIEDLCEFKEKVYPEE

Sequences:

>Translated_213_residues
MNQAKRIQILTRLRDNNPKPETELNFTSPFELLVAVTLSAQATDVSVNKATDKLFPVANTAHSIYALGVEGLKEYIKTIG
LYNNKAVNVIKACEILIEKYNGEVPEDREALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRLANRTKFAPGKNVVEVE
ERMLKVVPDEFKVDVHHWFILHGRYTCLARKPRCGSCIIEDLCEFKEKVYPEE
>Mature_213_residues
MNQAKRIQILTRLRDNNPKPETELNFTSPFELLVAVTLSAQATDVSVNKATDKLFPVANTAHSIYALGVEGLKEYIKTIG
LYNNKAVNVIKACEILIEKYNGEVPEDREALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRLANRTKFAPGKNVVEVE
ERMLKVVPDEFKVDVHHWFILHGRYTCLARKPRCGSCIIEDLCEFKEKVYPEE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=30.5084745762712, Blast_Score=91, Evalue=7e-19,
Organism=Escherichia coli, GI1787920, Length=209, Percent_Identity=80.8612440191388, Blast_Score=360, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=31.638418079096, Blast_Score=97, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=26.2032085561497, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45550361, Length=191, Percent_Identity=34.0314136125654, Blast_Score=101, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24012; Mature: 24012

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQAKRIQILTRLRDNNPKPETELNFTSPFELLVAVTLSAQATDVSVNKATDKLFPVANT
CCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCC
AHSIYALGVEGLKEYIKTIGLYNNKAVNVIKACEILIEKYNGEVPEDREALEALPGVGRK
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIFRLANRTKFAPGKNVVEVEERMLKVVPDEFKVDVHHWFI
HHHEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHEEEEEEEEE
LHGRYTCLARKPRCGSCIIEDLCEFKEKVYPEE
EECCEEEEECCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNQAKRIQILTRLRDNNPKPETELNFTSPFELLVAVTLSAQATDVSVNKATDKLFPVANT
CCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCC
AHSIYALGVEGLKEYIKTIGLYNNKAVNVIKACEILIEKYNGEVPEDREALEALPGVGRK
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIFRLANRTKFAPGKNVVEVEERMLKVVPDEFKVDVHHWFI
HHHEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHEEEEEEEEE
LHGRYTCLARKPRCGSCIIEDLCEFKEKVYPEE
EECCEEEEECCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]