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The map label for this gene is pykA [H]

Identifier: 120598952

GI number: 120598952

Start: 2469067

End: 2470506

Strand: Direct

Name: pykA [H]

Synonym: Sputw3181_2144

Alternate gene names: 120598952

Gene position: 2469067-2470506 (Clockwise)

Preceding gene: 120598951

Following gene: 120598953

Centisome position: 52.44

GC content: 47.43

Gene sequence:

>1440_bases
ATGTTCCGCAGAACCAAAATCGTCACCACACTCGGCCCCGCCACTGATCGTGATGATAATCTACGCCGCATTATCGCGGC
AGGTGCAAATGTTGTACGTCTTAACTTTTCCCACGGCTCCCCCGAAGATCATCTCAAACGAGCAACTCAAGCTCGTGAAA
TAGCAAAAGAATTAGGTGTTCACGTTGCTATTCTTGGTGACTTACAAGGGCCTAAAATCCGCGTTTCTACTTTCAAAGAC
AACAAGAAAGTTCAGCTCAAACTCGGTCAAGCCTATGTTTTAGATGCTGAGCTCGCTAAAGGTGAAGGTGACGAGACCCA
AGTCGGTATCGATTACAAGCAATTGCCAGACGACGTGACGGTCGGCGATATCCTGATGTTAGATGATGGCCGTGTTCAAT
TACGTGTTGAAGGTGTTGAAGGCCGTAAGGTTCACACCACAGTGACGGTTGCGGGTCCTTTATCAAACAATAAGGGGATT
AATAAGCAAGGTGGTGGTTTATCTGCCGCGGCGCTGACCGAAAAAGATAAAGCAGACATTCTGACGGCCGCAATGATCCA
AGTGGATTATTTAGCCGTGTCTTTCCCACGTAGTGGTGCTGATCTCGATTACGCCCGCGATCTTGCCCGTCAAGCAGGTT
GTAATGCACTGATTGTTGCTAAAGTTGAACGTGCAGAAGCCGTTGCCACTGATGAAGCGATGGATGATGTGATCTTAGCG
TCTGATGTTGTTATGGTTGCCCGTGGTGACTTAGGCGTTGAAATTGGTGATGCTGCACTCGTTGCAGTGCAAAAGAAACT
TATCGCCCGTTCACGTCAACTCAACAAAATCGTCATTACAGCGACACAAATGATGGAGTCGATGATTTCAAGCCCAATGC
CAACCCGCGCCGAAGTAATGGACGTGGCAAACGCTGTGCTCGACGGGACTGATGCCGTAATGTTATCAGCCGAAACCGCT
GCGGGAGATTTTCCTGAAGAAACCGTTAAGGCGATGGCAAATGTGTGTGTCGGTGCAGAGTCGCACCCAAGTGTTAAAGT
GTCTAAGCATAGATTAGATGCACGCTTTACTTCTGTGGAAGAGACCATTGCCCTATCGACTATGTATGCGGCAAACCATT
TAGAAGGTGTTAAAGCGATTATCGCCTTAACCGAATCGGGTGCAACACCTCAGATGATGTCGCGCATCAGTTCTTCACTG
CCCATCCTTGGTTTATCACGCCATGCGACAACACTCGCTAAGATGGCATTATATCGCGGTGTATTACCGATTTACTTTGA
TTCAACTATCTATCCAGCCGATCTATTAGCACAAAAAGCCTTAGAGTCACTGACAACCGCCGGTTATTTACAAAGCGGCG
ATTTAGTTTTGATGACCAAGGGCGATGCAATGGAAACTATCGGTGGCACTAACACTTGTAAGGTGTTGATTGTTGCTTAA

Upstream 100 bases:

>100_bases
GAAACATAACTTTAGATCAATTTGTTTGTAACTTTACTACATAATTTGGAATGGCATGGTAATATAGTCTCAAGATTTTT
TAACTTTAACGGAGTATCTT

Downstream 100 bases:

>100_bases
GTTTCTTATCGTCACTAAAACAGTGTTAGAAAATTAGCCAATAAAAAACCACCTAATGAAGGCTGTCTTTTATACAGAAG
TCCCTCATATGATATGAGGG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGSPEDHLKRATQAREIAKELGVHVAILGDLQGPKIRVSTFKD
NKKVQLKLGQAYVLDAELAKGEGDETQVGIDYKQLPDDVTVGDILMLDDGRVQLRVEGVEGRKVHTTVTVAGPLSNNKGI
NKQGGGLSAAALTEKDKADILTAAMIQVDYLAVSFPRSGADLDYARDLARQAGCNALIVAKVERAEAVATDEAMDDVILA
SDVVMVARGDLGVEIGDAALVAVQKKLIARSRQLNKIVITATQMMESMISSPMPTRAEVMDVANAVLDGTDAVMLSAETA
AGDFPEETVKAMANVCVGAESHPSVKVSKHRLDARFTSVEETIALSTMYAANHLEGVKAIIALTESGATPQMMSRISSSL
PILGLSRHATTLAKMALYRGVLPIYFDSTIYPADLLAQKALESLTTAGYLQSGDLVLMTKGDAMETIGGTNTCKVLIVA

Sequences:

>Translated_479_residues
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGSPEDHLKRATQAREIAKELGVHVAILGDLQGPKIRVSTFKD
NKKVQLKLGQAYVLDAELAKGEGDETQVGIDYKQLPDDVTVGDILMLDDGRVQLRVEGVEGRKVHTTVTVAGPLSNNKGI
NKQGGGLSAAALTEKDKADILTAAMIQVDYLAVSFPRSGADLDYARDLARQAGCNALIVAKVERAEAVATDEAMDDVILA
SDVVMVARGDLGVEIGDAALVAVQKKLIARSRQLNKIVITATQMMESMISSPMPTRAEVMDVANAVLDGTDAVMLSAETA
AGDFPEETVKAMANVCVGAESHPSVKVSKHRLDARFTSVEETIALSTMYAANHLEGVKAIIALTESGATPQMMSRISSSL
PILGLSRHATTLAKMALYRGVLPIYFDSTIYPADLLAQKALESLTTAGYLQSGDLVLMTKGDAMETIGGTNTCKVLIVA
>Mature_479_residues
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGSPEDHLKRATQAREIAKELGVHVAILGDLQGPKIRVSTFKD
NKKVQLKLGQAYVLDAELAKGEGDETQVGIDYKQLPDDVTVGDILMLDDGRVQLRVEGVEGRKVHTTVTVAGPLSNNKGI
NKQGGGLSAAALTEKDKADILTAAMIQVDYLAVSFPRSGADLDYARDLARQAGCNALIVAKVERAEAVATDEAMDDVILA
SDVVMVARGDLGVEIGDAALVAVQKKLIARSRQLNKIVITATQMMESMISSPMPTRAEVMDVANAVLDGTDAVMLSAETA
AGDFPEETVKAMANVCVGAESHPSVKVSKHRLDARFTSVEETIALSTMYAANHLEGVKAIIALTESGATPQMMSRISSSL
PILGLSRHATTLAKMALYRGVLPIYFDSTIYPADLLAQKALESLTTAGYLQSGDLVLMTKGDAMETIGGTNTCKVLIVA

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=493, Percent_Identity=34.6855983772819, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI33286420, Length=493, Percent_Identity=34.6855983772819, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI33286418, Length=493, Percent_Identity=34.6855983772819, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI32967597, Length=494, Percent_Identity=34.2105263157895, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI10835121, Length=494, Percent_Identity=34.2105263157895, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=33.9100346020761, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=33.9100346020761, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=31.9148936170213, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=31.9148936170213, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=31.3084112149533, Blast_Score=93, Evalue=7e-19,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=71.6981132075472, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=482, Percent_Identity=36.0995850622407, Blast_Score=274, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=36.3449691991786, Blast_Score=271, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI71984406, Length=493, Percent_Identity=33.4685598377282, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI71984413, Length=493, Percent_Identity=33.4685598377282, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17506831, Length=493, Percent_Identity=33.4685598377282, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17506829, Length=493, Percent_Identity=33.4685598377282, Blast_Score=223, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319279, Length=477, Percent_Identity=35.4297693920335, Blast_Score=246, Evalue=8e-66,
Organism=Saccharomyces cerevisiae, GI6324923, Length=485, Percent_Identity=32.1649484536082, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24648964, Length=498, Percent_Identity=34.5381526104418, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI28571814, Length=498, Percent_Identity=34.5381526104418, Blast_Score=246, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24648966, Length=453, Percent_Identity=34.878587196468, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24581235, Length=497, Percent_Identity=31.7907444668008, Blast_Score=191, Evalue=9e-49,
Organism=Drosophila melanogaster, GI24646914, Length=278, Percent_Identity=35.9712230215827, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50997; Mature: 50997

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGSPEDHLKRATQAREIAKELGV
CCCCEEEEEEECCCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
HVAILGDLQGPKIRVSTFKDNKKVQLKLGQAYVLDAELAKGEGDETQVGIDYKQLPDDVT
EEEEEECCCCCEEEEEEECCCCEEEEEECCEEEEECHHHCCCCCCEEECCCHHHCCCCCC
VGDILMLDDGRVQLRVEGVEGRKVHTTVTVAGPLSNNKGINKQGGGLSAAALTEKDKADI
CCEEEEEECCEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHH
LTAAMIQVDYLAVSFPRSGADLDYARDLARQAGCNALIVAKVERAEAVATDEAMDDVILA
HHHHHHEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHH
SDVVMVARGDLGVEIGDAALVAVQKKLIARSRQLNKIVITATQMMESMISSPMPTRAEVM
CCEEEEEECCCCEEECCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCHHHHH
DVANAVLDGTDAVMLSAETAAGDFPEETVKAMANVCVGAESHPSVKVSKHRLDARFTSVE
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHH
ETIALSTMYAANHLEGVKAIIALTESGATPQMMSRISSSLPILGLSRHATTLAKMALYRG
HHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCCEEEECHHHHHHHHHHHHHC
VLPIYFDSTIYPADLLAQKALESLTTAGYLQSGDLVLMTKGDAMETIGGTNTCKVLIVA
CCEEEECCCCCCHHHHHHHHHHHHHHHCCEECCCEEEEECCCCHHHCCCCCCEEEEEEC
>Mature Secondary Structure
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGSPEDHLKRATQAREIAKELGV
CCCCEEEEEEECCCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
HVAILGDLQGPKIRVSTFKDNKKVQLKLGQAYVLDAELAKGEGDETQVGIDYKQLPDDVT
EEEEEECCCCCEEEEEEECCCCEEEEEECCEEEEECHHHCCCCCCEEECCCHHHCCCCCC
VGDILMLDDGRVQLRVEGVEGRKVHTTVTVAGPLSNNKGINKQGGGLSAAALTEKDKADI
CCEEEEEECCEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHH
LTAAMIQVDYLAVSFPRSGADLDYARDLARQAGCNALIVAKVERAEAVATDEAMDDVILA
HHHHHHEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHH
SDVVMVARGDLGVEIGDAALVAVQKKLIARSRQLNKIVITATQMMESMISSPMPTRAEVM
CCEEEEEECCCCEEECCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCHHHHH
DVANAVLDGTDAVMLSAETAAGDFPEETVKAMANVCVGAESHPSVKVSKHRLDARFTSVE
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHH
ETIALSTMYAANHLEGVKAIIALTESGATPQMMSRISSSLPILGLSRHATTLAKMALYRG
HHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCCEEEECHHHHHHHHHHHHHC
VLPIYFDSTIYPADLLAQKALESLTTAGYLQSGDLVLMTKGDAMETIGGTNTCKVLIVA
CCEEEECCCCCCHHHHHHHHHHHHHHHCCEECCCEEEEECCCCHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]