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The map label for this gene is ybfF [H]

Identifier: 120598936

GI number: 120598936

Start: 2452140

End: 2452916

Strand: Direct

Name: ybfF [H]

Synonym: Sputw3181_2128

Alternate gene names: 120598936

Gene position: 2452140-2452916 (Clockwise)

Preceding gene: 120598933

Following gene: 120598937

Centisome position: 52.08

GC content: 45.05

Gene sequence:

>777_bases
ATGAATTATGTTTCCTCAGGTCAAGGACATGCGGTGCTAATAATCCATGGCCTGTTTGGTAATTTAGATAATCTCAAAGG
CTTAGGCCAAGTGTTAGAAAGCCAGCACCAAGTGATCCGCGTTGATGTGCCCAACCATGGGTTAAGTGAGCATTGGGATC
ATATGGATTATCCTCGCCTTGCCCATGCCATGATAGATTTGCTCGATAACCTTGACATCGCTCACGCGCACATCATCGGC
CACTCTATGGGCGGTAAAATCGCCATGGCCACGGCATTAGCCTTTCCAGAGCGCATTATCAGCATGGTCGCTGCCGATAT
TGCACCTGTCGCATATGAGCCTAGACACGATATTGTATTTGCTGCATTAGAAAGCCTGCCCTTAGAAGGCCATACCGACA
GACGCTTTGCCCTAAACCACTTAATCAACCATGGTATTGATGAAGCCACTGCCCAATTTTTGCTGAAAAACTTGCAACGT
ACCGATACTGGATTTCGTTGGAAAATGAACTTAAGTGGTTTAAAAACCTGTTATCCAAACATTATTGGCTGGCATAATCA
GCCACCGAACCCCGTGCTGAGCTATTCTGGCCCAAGTCTATTTATTCGTGGCGGTGATTCAAATTATGTCAATAGCGAAC
ATAGGGACGCGATTATGGCGCAATTCCCTAGTGCACAAGCAAAAACACTTGAAGGTTGTGGACATTGGTTGCATGCCCAA
AAACCTGCGATTTTTAATCGAATTGTGTCTGAATTTATTGACAAACAGGCAATGTAA

Upstream 100 bases:

>100_bases
AAGCAACCAAGTTAGTTCGGCTTAACCACAAGACTTGTCAACCTCATCATATTAGGGGCACAATTCGCGTCTCGAAAGCA
AACCTCCCATAGGAAAACCC

Downstream 100 bases:

>100_bases
ATGACAAGGATTCTCAACTCAAATCATATAGCAGCCTTCACGTTGATGTGATATATTCGCGCCTCTTTTGGCCTTAGAAC
ACTCTAGAGGACTAAGAAAG

Product: alpha/beta hydrolase fold domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNYVSSGQGHAVLIIHGLFGNLDNLKGLGQVLESQHQVIRVDVPNHGLSEHWDHMDYPRLAHAMIDLLDNLDIAHAHIIG
HSMGGKIAMATALAFPERIISMVAADIAPVAYEPRHDIVFAALESLPLEGHTDRRFALNHLINHGIDEATAQFLLKNLQR
TDTGFRWKMNLSGLKTCYPNIIGWHNQPPNPVLSYSGPSLFIRGGDSNYVNSEHRDAIMAQFPSAQAKTLEGCGHWLHAQ
KPAIFNRIVSEFIDKQAM

Sequences:

>Translated_258_residues
MNYVSSGQGHAVLIIHGLFGNLDNLKGLGQVLESQHQVIRVDVPNHGLSEHWDHMDYPRLAHAMIDLLDNLDIAHAHIIG
HSMGGKIAMATALAFPERIISMVAADIAPVAYEPRHDIVFAALESLPLEGHTDRRFALNHLINHGIDEATAQFLLKNLQR
TDTGFRWKMNLSGLKTCYPNIIGWHNQPPNPVLSYSGPSLFIRGGDSNYVNSEHRDAIMAQFPSAQAKTLEGCGHWLHAQ
KPAIFNRIVSEFIDKQAM
>Mature_258_residues
MNYVSSGQGHAVLIIHGLFGNLDNLKGLGQVLESQHQVIRVDVPNHGLSEHWDHMDYPRLAHAMIDLLDNLDIAHAHIIG
HSMGGKIAMATALAFPERIISMVAADIAPVAYEPRHDIVFAALESLPLEGHTDRRFALNHLINHGIDEATAQFLLKNLQR
TDTGFRWKMNLSGLKTCYPNIIGWHNQPPNPVLSYSGPSLFIRGGDSNYVNSEHRDAIMAQFPSAQAKTLEGCGHWLHAQ
KPAIFNRIVSEFIDKQAM

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200008, Length=251, Percent_Identity=35.4581673306773, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI23200012, Length=251, Percent_Identity=35.4581673306773, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI223941839, Length=245, Percent_Identity=31.0204081632653, Blast_Score=111, Evalue=7e-25,
Organism=Escherichia coli, GI1786902, Length=244, Percent_Identity=40.5737704918033, Blast_Score=181, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17508535, Length=254, Percent_Identity=31.8897637795276, Blast_Score=132, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17507063, Length=260, Percent_Identity=24.2307692307692, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321468, Length=266, Percent_Identity=29.3233082706767, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24640348, Length=260, Percent_Identity=30.7692307692308, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24646055, Length=257, Percent_Identity=24.124513618677, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 28654; Mature: 28654

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYVSSGQGHAVLIIHGLFGNLDNLKGLGQVLESQHQVIRVDVPNHGLSEHWDHMDYPRL
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCHHHH
AHAMIDLLDNLDIAHAHIIGHSMGGKIAMATALAFPERIISMVAADIAPVAYEPRHDIVF
HHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
AALESLPLEGHTDRRFALNHLINHGIDEATAQFLLKNLQRTDTGFRWKMNLSGLKTCYPN
HHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHH
IIGWHNQPPNPVLSYSGPSLFIRGGDSNYVNSEHRDAIMAQFPSAQAKTLEGCGHWLHAQ
HCCCCCCCCCCEEECCCCEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCC
KPAIFNRIVSEFIDKQAM
CCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNYVSSGQGHAVLIIHGLFGNLDNLKGLGQVLESQHQVIRVDVPNHGLSEHWDHMDYPRL
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCHHHH
AHAMIDLLDNLDIAHAHIIGHSMGGKIAMATALAFPERIISMVAADIAPVAYEPRHDIVF
HHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
AALESLPLEGHTDRRFALNHLINHGIDEATAQFLLKNLQRTDTGFRWKMNLSGLKTCYPN
HHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHH
IIGWHNQPPNPVLSYSGPSLFIRGGDSNYVNSEHRDAIMAQFPSAQAKTLEGCGHWLHAQ
HCCCCCCCCCCEEECCCCEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCC
KPAIFNRIVSEFIDKQAM
CCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]