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The map label for this gene is aroA [H]

Identifier: 120598783

GI number: 120598783

Start: 2283324

End: 2284604

Strand: Direct

Name: aroA [H]

Synonym: Sputw3181_1969

Alternate gene names: 120598783

Gene position: 2283324-2284604 (Clockwise)

Preceding gene: 120598781

Following gene: 120598784

Centisome position: 48.49

GC content: 48.56

Gene sequence:

>1281_bases
ATGAAGCAATTACGTCTTGAACCTGTTGTGCAAGTCCGCGGTGAAATCAATATTCCGGGCTCAAAAAGTATTTCAAATCG
AGCCTTATTATTGGCCACCTTAGCCCAAGGTACGACCACGCTCACCAATCTGCTCGACTCAGATGATATCCGCCACATGT
TGGCCTCCCTCAAGCAGTTAGGGGTGAACTACCGTTTATCGCAAAACAATACTGTGTGTGAACTCGATGGTTTAGGTGGT
GTGATTTCCTCTGCATCCGCACAGGAATTGTTTTTAGGCAATGCGGGTACCGCAATGCGCCCCTTATGCGCCGCCTTAAC
CTTAGGCCAAGGTGAATTTACCCTCACGGGCGAACCACGTATGGAAGAGCGTCCGATTGGCGATTTAGTCGATGCCTTAA
GGAAACTCGGTGCCAATGTCGTTTACCTTAAAAATGACGGTTTCCCGCCACTGACGATTAATGCCACTGGCCTTAATGGC
GGCGATGTCGAAATCGCAGGCGATTTATCGAGCCAGTTTTTAACGGCCTTATTGATGGTGGCACCGCTTGCTAAGGGCAG
CGTCAATATCCATGTCAAAGGCGAGTTAGTCTCTAAGCCTTATATCGATATCACTATTGCCTTGATGGCGCAGTTTGGCG
TTAACGTCATTAACCATGATTATGCCCGTTTTGAGATTGTTGCAGGCCAGCGTTATATATCACCGTGTAAAGTACTGGTA
GAAGGTGATGCGTCATCGGCATCGTATTTTCTCGCGGCAGGGGCGATTAAAGGCGGCGAAGTCAAAGTCACTGGTGTGGG
CCGTTTGAGTATCCAAGGCGATGTGAAATTTGCCGATGTGCTCGAAAAAATGGGCGCAGATATCGAGTGGGGCGATGACT
ATATTATCGCCCGCGGCTCACCTTTAACGGCCGTCGACTTAGACATGAACCATATTCCCGATGCGGCCATGACCATTGCA
ACTGCTGCGTTATTTGCCAAAGGCACAACGGTTATTCGCAATATCTACAACTGGCGCATTAAAGAAACCGACCGGTTAGC
GGCCATGGCGACCGAATTACGCAAAGTGGGCGCCGAAGTTGAAGAGGGCAATGATTACATTAAAATCACTCCACCGGCGG
TGATTAACACCGCTGAAATCGATACCTACAACGATCATCGCATGGCCATGTGCTTTTCTATGTTGGCATTTGCCGATTGC
GGTATCACCATCAATGATCCTGACTGTACTTCCAAAACATTCCCTGACTACTTTAAGCAGTTTGCGAGCTTGCAAGGTTA
A

Upstream 100 bases:

>100_bases
GTAATCTTAACTTGACGCGCTGTAGCGTGAAGTTGCCATTTTGCGTATTCAGTCCGCGTGATCGTGATGATGACGCCCTA
TAGAGAAAGAAGATTTATTC

Downstream 100 bases:

>100_bases
GTTTTTGGGTTTCGCCATGGTTACAATAATCGATGTCAAGAATAGCAAGCCGTGTTTGCGCGGCTTCTAATCTTCACAAA
AAATCAACGGATTTTGTTTG

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKQLRLEPVVQVRGEINIPGSKSISNRALLLATLAQGTTTLTNLLDSDDIRHMLASLKQLGVNYRLSQNNTVCELDGLGG
VISSASAQELFLGNAGTAMRPLCAALTLGQGEFTLTGEPRMEERPIGDLVDALRKLGANVVYLKNDGFPPLTINATGLNG
GDVEIAGDLSSQFLTALLMVAPLAKGSVNIHVKGELVSKPYIDITIALMAQFGVNVINHDYARFEIVAGQRYISPCKVLV
EGDASSASYFLAAGAIKGGEVKVTGVGRLSIQGDVKFADVLEKMGADIEWGDDYIIARGSPLTAVDLDMNHIPDAAMTIA
TAALFAKGTTVIRNIYNWRIKETDRLAAMATELRKVGAEVEEGNDYIKITPPAVINTAEIDTYNDHRMAMCFSMLAFADC
GITINDPDCTSKTFPDYFKQFASLQG

Sequences:

>Translated_426_residues
MKQLRLEPVVQVRGEINIPGSKSISNRALLLATLAQGTTTLTNLLDSDDIRHMLASLKQLGVNYRLSQNNTVCELDGLGG
VISSASAQELFLGNAGTAMRPLCAALTLGQGEFTLTGEPRMEERPIGDLVDALRKLGANVVYLKNDGFPPLTINATGLNG
GDVEIAGDLSSQFLTALLMVAPLAKGSVNIHVKGELVSKPYIDITIALMAQFGVNVINHDYARFEIVAGQRYISPCKVLV
EGDASSASYFLAAGAIKGGEVKVTGVGRLSIQGDVKFADVLEKMGADIEWGDDYIIARGSPLTAVDLDMNHIPDAAMTIA
TAALFAKGTTVIRNIYNWRIKETDRLAAMATELRKVGAEVEEGNDYIKITPPAVINTAEIDTYNDHRMAMCFSMLAFADC
GITINDPDCTSKTFPDYFKQFASLQG
>Mature_426_residues
MKQLRLEPVVQVRGEINIPGSKSISNRALLLATLAQGTTTLTNLLDSDDIRHMLASLKQLGVNYRLSQNNTVCELDGLGG
VISSASAQELFLGNAGTAMRPLCAALTLGQGEFTLTGEPRMEERPIGDLVDALRKLGANVVYLKNDGFPPLTINATGLNG
GDVEIAGDLSSQFLTALLMVAPLAKGSVNIHVKGELVSKPYIDITIALMAQFGVNVINHDYARFEIVAGQRYISPCKVLV
EGDASSASYFLAAGAIKGGEVKVTGVGRLSIQGDVKFADVLEKMGADIEWGDDYIIARGSPLTAVDLDMNHIPDAAMTIA
TAALFAKGTTVIRNIYNWRIKETDRLAAMATELRKVGAEVEEGNDYIKITPPAVINTAEIDTYNDHRMAMCFSMLAFADC
GITINDPDCTSKTFPDYFKQFASLQG

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1787137, Length=424, Percent_Identity=66.9811320754717, Blast_Score=568, Evalue=1e-163,
Organism=Saccharomyces cerevisiae, GI6320332, Length=451, Percent_Identity=37.9157427937916, Blast_Score=251, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792 [H]

Pfam domain/function: PF00275 EPSP_synthase [H]

EC number: =2.5.1.19 [H]

Molecular weight: Translated: 45712; Mature: 45712

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00104 EPSP_SYNTHASE_1 ; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLRLEPVVQVRGEINIPGSKSISNRALLLATLAQGTTTLTNLLDSDDIRHMLASLKQL
CCCCCCCCCEEECCEEECCCCCCCCCCEEEEEEHHCCHHHHHHHHCCHHHHHHHHHHHHC
GVNYRLSQNNTVCELDGLGGVISSASAQELFLGNAGTAMRPLCAALTLGQGEFTLTGEPR
CCEEEECCCCCEEEECCCCCHHCCCCCCCEEECCCCCHHHHHHHHHEECCCCEEECCCCC
MEERPIGDLVDALRKLGANVVYLKNDGFPPLTINATGLNGGDVEIAGDLSSQFLTALLMV
CCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHH
APLAKGSVNIHVKGELVSKPYIDITIALMAQFGVNVINHDYARFEIVAGQRYISPCKVLV
HHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCHHHCCCCEEEEEEECCCCCCCEEEEE
EGDASSASYFLAAGAIKGGEVKVTGVGRLSIQGDVKFADVLEKMGADIEWGDDYIIARGS
ECCCCCCEEEEEECCCCCCEEEEEEEEEEEEECCCHHHHHHHHHCCCCCCCCCEEEECCC
PLTAVDLDMNHIPDAAMTIATAALFAKGTTVIRNIYNWRIKETDRLAAMATELRKVGAEV
CCEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCEECCHHHHHHHHHHHHHHCCCH
EEGNDYIKITPPAVINTAEIDTYNDHRMAMCFSMLAFADCGITINDPDCTSKTFPDYFKQ
HCCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHH
FASLQG
HHCCCC
>Mature Secondary Structure
MKQLRLEPVVQVRGEINIPGSKSISNRALLLATLAQGTTTLTNLLDSDDIRHMLASLKQL
CCCCCCCCCEEECCEEECCCCCCCCCCEEEEEEHHCCHHHHHHHHCCHHHHHHHHHHHHC
GVNYRLSQNNTVCELDGLGGVISSASAQELFLGNAGTAMRPLCAALTLGQGEFTLTGEPR
CCEEEECCCCCEEEECCCCCHHCCCCCCCEEECCCCCHHHHHHHHHEECCCCEEECCCCC
MEERPIGDLVDALRKLGANVVYLKNDGFPPLTINATGLNGGDVEIAGDLSSQFLTALLMV
CCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHH
APLAKGSVNIHVKGELVSKPYIDITIALMAQFGVNVINHDYARFEIVAGQRYISPCKVLV
HHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCHHHCCCCEEEEEEECCCCCCCEEEEE
EGDASSASYFLAAGAIKGGEVKVTGVGRLSIQGDVKFADVLEKMGADIEWGDDYIIARGS
ECCCCCCEEEEEECCCCCCEEEEEEEEEEEEECCCHHHHHHHHHCCCCCCCCCEEEECCC
PLTAVDLDMNHIPDAAMTIATAALFAKGTTVIRNIYNWRIKETDRLAAMATELRKVGAEV
CCEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCEECCHHHHHHHHHHHHHHCCCH
EEGNDYIKITPPAVINTAEIDTYNDHRMAMCFSMLAFADCGITINDPDCTSKTFPDYFKQ
HCCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHH
FASLQG
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA