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The map label for this gene is ilvD [H]

Identifier: 120598765

GI number: 120598765

Start: 2255638

End: 2257371

Strand: Direct

Name: ilvD [H]

Synonym: Sputw3181_1950

Alternate gene names: 120598765

Gene position: 2255638-2257371 (Clockwise)

Preceding gene: 120598763

Following gene: 120598766

Centisome position: 47.91

GC content: 44.87

Gene sequence:

>1734_bases
ATGCAGAATAAAAAAACAAAAGCACTTCGTTCCGCAAGCTGGTTTGGTAGCGATGATAAAAATGGTTTTATGTATCGTAG
TTGGATGAAAAACCAAGGCATTCCAGATCATCATTTTCAAAACAGACCTGTAATTGGTATTTGCAATACGTGGTCAGAGT
TAACGCCTTGTAATGGTCATCTACGTGAGTTGGCTGAAAGAGTTAAAAATGGGATCCGTGAAGCAGGGGGGATCCCAGTG
GAATTTCCAGTATTTTCCAATGGTGAATCTAATTTACGCCCCAGTGCCATGTTAACGCGTAATTTGGCCGCGATGGATAC
CGAAGAAGCGATTCGAGGCAATCCGATTGATGGTGTGGTGCTCTTAGTGGGTTGTGATAAAACAACGCCGGCTTTACTTA
TGGGGGCGGCCAGTTGCAACTTACCCACCATAGTCGTGACGGGTGGGCCTATGCTAAATGGTAAACATAAAGGGAAAGAT
GTAGGGTCTGGCACTTTAGTCTGGGAACTGCATCAAGAATATAAAGCAGGAAATATTAGCTTGGCTGAGTTTATGAATGC
CGAAGCTGATATGTCACGTTCAACGGGTACTTGTAATACCATGGGAACGGCATCGACTATGGCCTGCATGGCAGAAAGTT
TAGGCACCAGTTTGCCACAAAATGCGGCTATTCCTGCTGTGGATTCAAGACGTTATGTCTTAGCCCATATGTCTGGAATG
CGTATTGTCGATATGGTCCATGAGGATTTAACGCTTTCAAAAGTGCTCACGCGTGAAGCATTTATCAATGCGATAAAAAC
CAATGCGGCGATTGGCGGCTCGACCAATGCGGTTATCCACTTAAAAGCCATAGCGGGGCGAATAGGGGTTGATTTGTCAC
TGGATGATTGGTCGTATGGCTATGATGTACCGACGATAGTCAATCTTAAGCCCTCGGGTCAGTATTTGATGGAGGATTTT
TACTATGCAGGAGGTTTACCCGCAGTGCTAAAAGAACTCTTTAACCATCATTTATTGAGTAAAAATACCTTAACAGTGAA
TGGGAAAACGCTCTGGGAAAATGTGGCAAATGCACCTTGTTACAACCGAGACGTGATCATGAGTATTGATACACCCTTAG
TTGAAAATGGTGGGATCAGAGTATTAAAGGGAAACTTGGCCCCTCGAGGTGCAGTGATTAAACCTTCTGCAGCCAGTCCG
CATTTAATGAAACATCGTGGTAAAGCGGTCGTGTTTGAGAGCTTTGATGATTACAACGCCCGCATAAATGATCCCGAACT
TGCGATTGATGAAACGAGCATCATGGTGCTCAAAAATTGCGGCCCGAAGGGATATCCTGGAATGGCTGAGGTCGGCAATA
TGGGATTGCCACCTAAGCTGTTAAAAAAAGGGATTAAAGATATGGTTAGGATTTCGGATGCGCGAATGAGTGGTACTGCA
TTTGGAACAGTCGTATTGCATGTTGTCCCCGAGTCACAGGACTTAGGCCCACTAGCTGCGGTACAAAATGGTGATGTGAT
CGCACTCGATACTTTTGCTGGCGTACTGCAACTAGAGATTAGTGACGAAGAATTAGCGGATCGGTTAGCAAAAATCGCCT
TGGTTAAGCAACTTCCTGTCAGCAGTGGTTATTTATCCCTTTTTAGGGAAAGAGTGTTGCAGGCCGATGAAGGGTGTGAT
TTTGATTTTCTAGTGGGATGTCGGGGTGCTGATATTCCGGCACATTCCCATTAA

Upstream 100 bases:

>100_bases
CCATTATTTGCCTTAGCTTCGCGGTAAATACCGCACGTACTGAGAATAGGTGTGAATAGGCGAGTCGGGCACTAAGCAGA
GGTAAAACGAAGGTGTAACA

Downstream 100 bases:

>100_bases
ACATGGACACTTTTTCTTTTATAATAAAACCAAATAATTATTTTAATAAAGGTTGAGTTATGAAGTTAAGTAATCAATAC
CCGAGCTTGAAAGGAAAAAC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MQNKKTKALRSASWFGSDDKNGFMYRSWMKNQGIPDHHFQNRPVIGICNTWSELTPCNGHLRELAERVKNGIREAGGIPV
EFPVFSNGESNLRPSAMLTRNLAAMDTEEAIRGNPIDGVVLLVGCDKTTPALLMGAASCNLPTIVVTGGPMLNGKHKGKD
VGSGTLVWELHQEYKAGNISLAEFMNAEADMSRSTGTCNTMGTASTMACMAESLGTSLPQNAAIPAVDSRRYVLAHMSGM
RIVDMVHEDLTLSKVLTREAFINAIKTNAAIGGSTNAVIHLKAIAGRIGVDLSLDDWSYGYDVPTIVNLKPSGQYLMEDF
YYAGGLPAVLKELFNHHLLSKNTLTVNGKTLWENVANAPCYNRDVIMSIDTPLVENGGIRVLKGNLAPRGAVIKPSAASP
HLMKHRGKAVVFESFDDYNARINDPELAIDETSIMVLKNCGPKGYPGMAEVGNMGLPPKLLKKGIKDMVRISDARMSGTA
FGTVVLHVVPESQDLGPLAAVQNGDVIALDTFAGVLQLEISDEELADRLAKIALVKQLPVSSGYLSLFRERVLQADEGCD
FDFLVGCRGADIPAHSH

Sequences:

>Translated_577_residues
MQNKKTKALRSASWFGSDDKNGFMYRSWMKNQGIPDHHFQNRPVIGICNTWSELTPCNGHLRELAERVKNGIREAGGIPV
EFPVFSNGESNLRPSAMLTRNLAAMDTEEAIRGNPIDGVVLLVGCDKTTPALLMGAASCNLPTIVVTGGPMLNGKHKGKD
VGSGTLVWELHQEYKAGNISLAEFMNAEADMSRSTGTCNTMGTASTMACMAESLGTSLPQNAAIPAVDSRRYVLAHMSGM
RIVDMVHEDLTLSKVLTREAFINAIKTNAAIGGSTNAVIHLKAIAGRIGVDLSLDDWSYGYDVPTIVNLKPSGQYLMEDF
YYAGGLPAVLKELFNHHLLSKNTLTVNGKTLWENVANAPCYNRDVIMSIDTPLVENGGIRVLKGNLAPRGAVIKPSAASP
HLMKHRGKAVVFESFDDYNARINDPELAIDETSIMVLKNCGPKGYPGMAEVGNMGLPPKLLKKGIKDMVRISDARMSGTA
FGTVVLHVVPESQDLGPLAAVQNGDVIALDTFAGVLQLEISDEELADRLAKIALVKQLPVSSGYLSLFRERVLQADEGCD
FDFLVGCRGADIPAHSH
>Mature_577_residues
MQNKKTKALRSASWFGSDDKNGFMYRSWMKNQGIPDHHFQNRPVIGICNTWSELTPCNGHLRELAERVKNGIREAGGIPV
EFPVFSNGESNLRPSAMLTRNLAAMDTEEAIRGNPIDGVVLLVGCDKTTPALLMGAASCNLPTIVVTGGPMLNGKHKGKD
VGSGTLVWELHQEYKAGNISLAEFMNAEADMSRSTGTCNTMGTASTMACMAESLGTSLPQNAAIPAVDSRRYVLAHMSGM
RIVDMVHEDLTLSKVLTREAFINAIKTNAAIGGSTNAVIHLKAIAGRIGVDLSLDDWSYGYDVPTIVNLKPSGQYLMEDF
YYAGGLPAVLKELFNHHLLSKNTLTVNGKTLWENVANAPCYNRDVIMSIDTPLVENGGIRVLKGNLAPRGAVIKPSAASP
HLMKHRGKAVVFESFDDYNARINDPELAIDETSIMVLKNCGPKGYPGMAEVGNMGLPPKLLKKGIKDMVRISDARMSGTA
FGTVVLHVVPESQDLGPLAAVQNGDVIALDTFAGVLQLEISDEELADRLAKIALVKQLPVSSGYLSLFRERVLQADEGCD
FDFLVGCRGADIPAHSH

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=557, Percent_Identity=30.3411131059246, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1788157, Length=546, Percent_Identity=29.3040293040293, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI2367371, Length=476, Percent_Identity=30.2521008403361, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1786464, Length=509, Percent_Identity=29.4695481335953, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6322476, Length=577, Percent_Identity=32.0623916811092, Blast_Score=256, Evalue=5e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 62274; Mature: 62274

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00886 ILVD_EDD_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKKTKALRSASWFGSDDKNGFMYRSWMKNQGIPDHHFQNRPVIGICNTWSELTPCNGH
CCCCHHHHHHHHCCCCCCCCCCCEEEHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCHH
LRELAERVKNGIREAGGIPVEFPVFSNGESNLRPSAMLTRNLAAMDTEEAIRGNPIDGVV
HHHHHHHHHHHHHHHCCCCEEECEECCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCEE
LLVGCDKTTPALLMGAASCNLPTIVVTGGPMLNGKHKGKDVGSGTLVWELHQEYKAGNIS
EEEECCCCCHHHEEECCCCCCCEEEEECCCEECCCCCCCCCCCCCEEEEEHHHHCCCCEE
LAEFMNAEADMSRSTGTCNTMGTASTMACMAESLGTSLPQNAAIPAVDSRRYVLAHMSGM
HHHHHCCHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCC
RIVDMVHEDLTLSKVLTREAFINAIKTNAAIGGSTNAVIHLKAIAGRIGVDLSLDDWSYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCC
YDVPTIVNLKPSGQYLMEDFYYAGGLPAVLKELFNHHLLSKNTLTVNGKTLWENVANAPC
CCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCC
YNRDVIMSIDTPLVENGGIRVLKGNLAPRGAVIKPSAASPHLMKHRGKAVVFESFDDYNA
CCCCEEEEECCCEECCCCEEEEECCCCCCCCEECCCCCCCHHHHHCCCEEEEECCCCCCC
RINDPELAIDETSIMVLKNCGPKGYPGMAEVGNMGLPPKLLKKGIKDMVRISDARMSGTA
EECCCCEEECCCEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCE
FGTVVLHVVPESQDLGPLAAVQNGDVIALDTFAGVLQLEISDEELADRLAKIALVKQLPV
EEEEEEEEECCCCCCCCEEEECCCCEEEEECCCEEEEEEECHHHHHHHHHHHHHHHHCCC
SSGYLSLFRERVLQADEGCDFDFLVGCRGADIPAHSH
CCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MQNKKTKALRSASWFGSDDKNGFMYRSWMKNQGIPDHHFQNRPVIGICNTWSELTPCNGH
CCCCHHHHHHHHCCCCCCCCCCCEEEHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCHH
LRELAERVKNGIREAGGIPVEFPVFSNGESNLRPSAMLTRNLAAMDTEEAIRGNPIDGVV
HHHHHHHHHHHHHHHCCCCEEECEECCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCEE
LLVGCDKTTPALLMGAASCNLPTIVVTGGPMLNGKHKGKDVGSGTLVWELHQEYKAGNIS
EEEECCCCCHHHEEECCCCCCCEEEEECCCEECCCCCCCCCCCCCEEEEEHHHHCCCCEE
LAEFMNAEADMSRSTGTCNTMGTASTMACMAESLGTSLPQNAAIPAVDSRRYVLAHMSGM
HHHHHCCHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCC
RIVDMVHEDLTLSKVLTREAFINAIKTNAAIGGSTNAVIHLKAIAGRIGVDLSLDDWSYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCC
YDVPTIVNLKPSGQYLMEDFYYAGGLPAVLKELFNHHLLSKNTLTVNGKTLWENVANAPC
CCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCC
YNRDVIMSIDTPLVENGGIRVLKGNLAPRGAVIKPSAASPHLMKHRGKAVVFESFDDYNA
CCCCEEEEECCCEECCCCEEEEECCCCCCCCEECCCCCCCHHHHHCCCEEEEECCCCCCC
RINDPELAIDETSIMVLKNCGPKGYPGMAEVGNMGLPPKLLKKGIKDMVRISDARMSGTA
EECCCCEEECCCEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCE
FGTVVLHVVPESQDLGPLAAVQNGDVIALDTFAGVLQLEISDEELADRLAKIALVKQLPV
EEEEEEEEECCCCCCCCEEEECCCCEEEEECCCEEEEEEECHHHHHHHHHHHHHHHHCCC
SSGYLSLFRERVLQADEGCDFDFLVGCRGADIPAHSH
CCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA