Click here to switch to the map view.

The map label for this gene is tnpA [H]

Identifier: 120598584

GI number: 120598584

Start: 2038033

End: 2039406

Strand: Direct

Name: tnpA [H]

Synonym: Sputw3181_1767

Alternate gene names: 120598584

Gene position: 2038033-2039406 (Clockwise)

Preceding gene: 120598583

Following gene: 120598585

Centisome position: 43.29

GC content: 44.25

Gene sequence:

>1374_bases
ATGATAAAAGGCAATAATGATTGGGCTGAAGAACAATTTGGTCATGCTAAACTTGGTGACCCAAGAAGAACGGCTAGGCT
CGTAAAAATGGCGTCAGACTTAGCTCAGCATCCCGGTAAGTCGGTAGTAAAATCGTCACCTTCACCAGCAAGTATGGAGG
GCGCTTACCGGTTTATTCGAAACGATAATGTATCAGCAGACGATATTGCTGAAGCCGGTTTTCGTGCGACAGTCAATCAA
GCCCACCGTTACCCCCTCCTTCTCGCTATTGAAGATACAACCACCTTAAGCTACAAGCATCGCTCTATTCGAGCGGACTT
AGGACATGTCAACCAAGGTAATCGTTATCGAGGTCTGCTTGCCCATAGTATTTTGTTGTTTGCTCCTGAAACTCTTGACG
TTGTTGGATTGATTGAACAACACAGATGGACCAGAGACATCAAGACTCGAGGTATCCGCCGTGAGAATTTAAAACGACCT
TACGAAGAAAAAGAAGGTTATAAATGGGAAAGTGCATCACGTAATATGGCTGCACGTCTAGGAACCGCGATGGCTAACGT
GATATCGGTTTGTGATCGTGAAGCCGACATCTACGATTATCTCCTTTACAAGATAGCGAACCAACAACGTTTTGTTGTGC
GGTCAATGATGAGTCGTCATATAGAAGAAGGTTCAGATAAGCTTTATCATTTTGCATCAGAGCTTAACAGTGTGAAACAA
CGCCAAATCCAGATAGCTCAAAGGGGCGGTCGTAAAGCTCGAGAAGTCACGCTGGATGTTAAATATGCGGCAGTAACGTT
AAAAACCCCGGCTAACAAAAAAGGCTCCCCCATTTCTCTGAACTATGTGGGTTGCTCCGAAGTCGGTGATGAAGAGAAGA
CGCTCAATTGGCATATTCTGACCAATGAGCCAGTAAACAGTGCCGAAGATGCTTTAAAAATTATCGGTTATTACGAGAAG
CGCTGGCTGATTGAGGAGTATCATAAGGTCTGGAAAAGTGAAGGGACCGGAGTTGAAGACCTTCGAGTACAGAGCAAAGA
TAACTTAGATAGATTAGCGACTATTTATGCCTTTTTAGCGGTAAGGATCTTCCAATTGAAATTTGCCAATGAGCAAATCA
AAGACATTAGCAGTGAGAAAATCTTGTCACCAAGAGCGTGGAAGTTACTTTGGCTTAAAAGAATAAAGACGCCACCTCCA
GAGGAAGTACCCACTGCAAAATGGGCATATGAGCACCTGGCAAGGCTTGGTGGTTGGAAAGACAGTAAAAGAAATGGAAG
AGCATCAGTAAAGACGCTCTGGGAAGGATGGCTCAAATTGCAAGCAATCCTTGAGGGTTACGAACTTGCTCTGTCTCTTG
AACAGGACTTGTGA

Upstream 100 bases:

>100_bases
TTTTATACAGAAGTCCCTCATATGATATGAGGGATTTTTTTTGAACTAAATTCTTGGCTCACGATCAGATCTTTAACGGC
TTTAATTGAGGCTCCCTGAG

Downstream 100 bases:

>100_bases
TCAAGAGACAGGCCGCAAGGCTGGTTTTTTATGGATGACGGAGGCTTAGATGGACAAGATGACTGACAGTATTCAACACA
CGCTCAAACAATTCGCCGCC

Product: transposase Tn5 dimerisation subunit

Products: NA

Alternate protein names: Tnp [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MIKGNNDWAEEQFGHAKLGDPRRTARLVKMASDLAQHPGKSVVKSSPSPASMEGAYRFIRNDNVSADDIAEAGFRATVNQ
AHRYPLLLAIEDTTTLSYKHRSIRADLGHVNQGNRYRGLLAHSILLFAPETLDVVGLIEQHRWTRDIKTRGIRRENLKRP
YEEKEGYKWESASRNMAARLGTAMANVISVCDREADIYDYLLYKIANQQRFVVRSMMSRHIEEGSDKLYHFASELNSVKQ
RQIQIAQRGGRKAREVTLDVKYAAVTLKTPANKKGSPISLNYVGCSEVGDEEKTLNWHILTNEPVNSAEDALKIIGYYEK
RWLIEEYHKVWKSEGTGVEDLRVQSKDNLDRLATIYAFLAVRIFQLKFANEQIKDISSEKILSPRAWKLLWLKRIKTPPP
EEVPTAKWAYEHLARLGGWKDSKRNGRASVKTLWEGWLKLQAILEGYELALSLEQDL

Sequences:

>Translated_457_residues
MIKGNNDWAEEQFGHAKLGDPRRTARLVKMASDLAQHPGKSVVKSSPSPASMEGAYRFIRNDNVSADDIAEAGFRATVNQ
AHRYPLLLAIEDTTTLSYKHRSIRADLGHVNQGNRYRGLLAHSILLFAPETLDVVGLIEQHRWTRDIKTRGIRRENLKRP
YEEKEGYKWESASRNMAARLGTAMANVISVCDREADIYDYLLYKIANQQRFVVRSMMSRHIEEGSDKLYHFASELNSVKQ
RQIQIAQRGGRKAREVTLDVKYAAVTLKTPANKKGSPISLNYVGCSEVGDEEKTLNWHILTNEPVNSAEDALKIIGYYEK
RWLIEEYHKVWKSEGTGVEDLRVQSKDNLDRLATIYAFLAVRIFQLKFANEQIKDISSEKILSPRAWKLLWLKRIKTPPP
EEVPTAKWAYEHLARLGGWKDSKRNGRASVKTLWEGWLKLQAILEGYELALSLEQDL
>Mature_457_residues
MIKGNNDWAEEQFGHAKLGDPRRTARLVKMASDLAQHPGKSVVKSSPSPASMEGAYRFIRNDNVSADDIAEAGFRATVNQ
AHRYPLLLAIEDTTTLSYKHRSIRADLGHVNQGNRYRGLLAHSILLFAPETLDVVGLIEQHRWTRDIKTRGIRRENLKRP
YEEKEGYKWESASRNMAARLGTAMANVISVCDREADIYDYLLYKIANQQRFVVRSMMSRHIEEGSDKLYHFASELNSVKQ
RQIQIAQRGGRKAREVTLDVKYAAVTLKTPANKKGSPISLNYVGCSEVGDEEKTLNWHILTNEPVNSAEDALKIIGYYEK
RWLIEEYHKVWKSEGTGVEDLRVQSKDNLDRLATIYAFLAVRIFQLKFANEQIKDISSEKILSPRAWKLLWLKRIKTPPP
EEVPTAKWAYEHLARLGGWKDSKRNGRASVKTLWEGWLKLQAILEGYELALSLEQDL

Specific function: Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After n

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559
- InterPro:   IPR003201
- InterPro:   IPR014737
- InterPro:   IPR014736
- InterPro:   IPR014735 [H]

Pfam domain/function: PF01609 Transposase_11; PF02281 Transposase_Tn5 [H]

EC number: NA

Molecular weight: Translated: 52276; Mature: 52276

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKGNNDWAEEQFGHAKLGDPRRTARLVKMASDLAQHPGKSVVKSSPSPASMEGAYRFIR
CCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHH
NDNVSADDIAEAGFRATVNQAHRYPLLLAIEDTTTLSYKHRSIRADLGHVNQGNRYRGLL
CCCCCHHHHHHHCHHHHHHHCCCCEEEEEEECCCEEHHHHHHHHHHCCCCCCCCCHHHHH
AHSILLFAPETLDVVGLIEQHRWTRDIKTRGIRRENLKRPYEEKEGYKWESASRNMAARL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHCCCCCCHHCHHHHHHH
GTAMANVISVCDREADIYDYLLYKIANQQRFVVRSMMSRHIEEGSDKLYHFASELNSVKQ
HHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RQIQIAQRGGRKAREVTLDVKYAAVTLKTPANKKGSPISLNYVGCSEVGDEEKTLNWHIL
HHHHHHHHCCCCCCEEEEEEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCEEEEEEE
TNEPVNSAEDALKIIGYYEKRWLIEEYHKVWKSEGTGVEDLRVQSKDNLDRLATIYAFLA
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCHHHHHHHHHHHH
VRIFQLKFANEQIKDISSEKILSPRAWKLLWLKRIKTPPPEEVPTAKWAYEHLARLGGWK
HHHHHHHHCCHHHHHCCHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
DSKRNGRASVKTLWEGWLKLQAILEGYELALSLEQDL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
>Mature Secondary Structure
MIKGNNDWAEEQFGHAKLGDPRRTARLVKMASDLAQHPGKSVVKSSPSPASMEGAYRFIR
CCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHH
NDNVSADDIAEAGFRATVNQAHRYPLLLAIEDTTTLSYKHRSIRADLGHVNQGNRYRGLL
CCCCCHHHHHHHCHHHHHHHCCCCEEEEEEECCCEEHHHHHHHHHHCCCCCCCCCHHHHH
AHSILLFAPETLDVVGLIEQHRWTRDIKTRGIRRENLKRPYEEKEGYKWESASRNMAARL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHCCCCCCHHCHHHHHHH
GTAMANVISVCDREADIYDYLLYKIANQQRFVVRSMMSRHIEEGSDKLYHFASELNSVKQ
HHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RQIQIAQRGGRKAREVTLDVKYAAVTLKTPANKKGSPISLNYVGCSEVGDEEKTLNWHIL
HHHHHHHHCCCCCCEEEEEEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCEEEEEEE
TNEPVNSAEDALKIIGYYEKRWLIEEYHKVWKSEGTGVEDLRVQSKDNLDRLATIYAFLA
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCHHHHHHHHHHHH
VRIFQLKFANEQIKDISSEKILSPRAWKLLWLKRIKTPPPEEVPTAKWAYEHLARLGGWK
HHHHHHHHCCHHHHHCCHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
DSKRNGRASVKTLWEGWLKLQAILEGYELALSLEQDL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6260374; 6271452; 7867940; 6291786; 10884228; 12367522; 11896402 [H]