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The map label for this gene is ppsA [H]

Identifier: 120598582

GI number: 120598582

Start: 2032326

End: 2034695

Strand: Direct

Name: ppsA [H]

Synonym: Sputw3181_1765

Alternate gene names: 120598582

Gene position: 2032326-2034695 (Clockwise)

Preceding gene: 120598577

Following gene: 120598583

Centisome position: 43.16

GC content: 46.75

Gene sequence:

>2370_bases
GTGCAGCAATACGTACTCTGGTATCAGGAATTAGGCATGGGTGACGTCAACTTAGTTGGCGGTAAAAATGCTTCTCTTGG
CGAGATGATCAGCAATCTAGCTAATGCCGGTGTTCAAGTACCTGGCGGATTTGCGACTACTTCTTATGCGTTCAATGAGT
TCCTTGAACAAAGTGGAGTAAACCAGAAGATTTATGACATCCTAGCCACATTGGATGTAGATGATGTCAATGCACTGGCA
AAAGTAGGTGCACAGATCAGACAATGGGTGATTGACACCCCATTCCAACCAGCTTTAGAGCAAGCCATTCGTGAAGCTTA
CGATAAACTTGCCGCTGAAACTAGCGATGCGTCATTTGCAGTACGTTCTTCTGCCACCGCAGAAGACATGCCAGATGCCT
CTTTTGCTGGTCAGCAAGAAACCTTCTTAAACGTGAAAGGATTTGACTCCGTTCTCGTGGCTATCAAGCACGTGTTCGCG
TCACTCTTTAACGACCGTGCGATTTCTTACCGTGTTCACCAAGGTTACGAACACCACGGTGTTGCTCTGTCTGCCGGCGT
ACAACGCATGGTTCGTTCAGACAAAGCGGCGTCAGGCGTGATGTTCACTATGGACACTGAATCTGGCAACAACGATGTCG
TCTTTATCACTTCCTCTTTCGGTCTGGGTGAAATGGTCGTTCAAGGTGCGGTGAACCCTGACGAATTCTATGTTCACAAG
CCGATTTTATCCCAAGGCCATAAAGCCGTTGTTCGCCGTAATATCGGTAGCAAGCTCATCCAGATGGTGTATTCCGATGA
TGCTGCTCACGGCAAACAAGTCAAAATTGAGGACGTTGCTGCCGATAAACGTCGTCAATTCTCCATCAATGATGCTGAAG
TGATGGAACTGGCTAAACAAGCCATGGTTATCGAAAAACACTACGGTCGTCCAATGGACATCGAGTGGGCGAAAGACGGT
AACGATGGCAAACTGTATATCGTGCAAGCGCGTCCTGAAACGGTTCGTAGCCGTGAAGATGTGCAACTGATTGAGCGTTA
TCACTTAAAGAGCCGTGGCGCGGTGATCTCTGAAGGTCGTGCTATTGGTCATAAAGTGGGTTCAGGTGTGGCTAAGGTAT
TAACCTCTATCGCCGATATGGATCAAATCCAACCTGGCGATGTGTTAGTGACTGACATGACCGACCCTGATTGGGAACCT
ATCATGAAGCGCGCCAGCGCCATCGTCACTAACCGTGGCGGTCGTACCTGTCATGCGGCGATTATTGCCCGTGAACTAGG
TGTACCAGCGGTAGTAGGTTGTGGTGACGTGACTGACCGTATCAAAAATGGCCAGTTAGTGACAGTGTCTTGCGCAGAAG
GTGATACTGGCTTTATTTACGAAGGTAAACAAGAGTTTGAAGTGATTTCAAACCGCGTTGACTCACTGCCAGCCTTACCA
ATGAAGATTATGATGAACGTGGGTAATCCTGATCGTGCATTTGATTTTGCCCGTTTGCCAAACGAAGGTGTGGGTCTAGC
GCGTTTAGAGTTCATTATCAACCGTATGATTGGTATTCACCCCAAAGCACTGCTTGAGTTTAACCAACAAGATGCAGCGC
TTCAGGAAGAAATCAACGAGATGATCGCAGGTTATGAATCACCTGTTGAATTCTACATTGCTCGTTTGGTTGAAGGTATC
GCCTCTATCGGTTCAGCTTTCTATCCGAAGAAGGTGATCGTGCGTATGTCAGACTTTAAGTCTAACGAGTACGCAAACTT
AGTTGGCGGTGACAGATACGAGCCAGAAGAAGAAAACCCAATGTTGGGCTTCCGCGGTGCAAGCCGTTATATCTCTGAAT
CTTTCCGTGATTGTTTTGCCCTAGAGTGTGAAGCGATCAAACGTGTCCGTAATGACATGGGACTGAAAAACGTTGAAGTT
ATGATCCCATTCGTTCGCACTGTGAAAGAAGCTGAGCAAGTTGTAGGTCTATTGGCTGAGCAAGGTTTAGAGCGTGGTAA
AGACGGTTTACGCGTCATTATGATGTGTGAAGTACCTTCAAACGCCTTGTTAGCAGATCAATTCCTTGAACACTTCGATG
GCTTCTCTATCGGCTCAAACGATTTAACTCAGCTAACGCTCGGTTTAGATCGTGACTCAGGCATTATTAGCCACCTGTTC
GATGAGCGTGATCAAGCTGTTAAGATATTATTATCGATGGCGATTAAAGCAGCTAAAGCGAAAGGTGCTTATATTGGTAT
ATGCGGTCAAGGCCCATCAGATCATGCTGATTTTGCTGGTTGGTTAGTGGAGCAGGGCATTGATACTGTGTCACTTAATC
CTGACACCGTAATTGATACATGGCTATATTTAGCTGAAGCTCACGGGTGA

Upstream 100 bases:

>100_bases
TACAAATAAAATTATAGATTTACGCTTCTTCGGCAGGAGTGTAAAATGCCGTTGCCCTCGTGTGAAGGGGCCACTGAAAT
AAACTTGCGGAGATAGAACT

Downstream 100 bases:

>100_bases
TCTTCGTTATAATTTTGTAAACATATAAAAAAAGCCGCTTTTTAGCGGCTTTTTTTTGTTTATAATGACGGCATAACAAT
AATTGACTGAGCCGCTCATG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 789; Mature: 789

Protein sequence:

>789_residues
MQQYVLWYQELGMGDVNLVGGKNASLGEMISNLANAGVQVPGGFATTSYAFNEFLEQSGVNQKIYDILATLDVDDVNALA
KVGAQIRQWVIDTPFQPALEQAIREAYDKLAAETSDASFAVRSSATAEDMPDASFAGQQETFLNVKGFDSVLVAIKHVFA
SLFNDRAISYRVHQGYEHHGVALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
PILSQGHKAVVRRNIGSKLIQMVYSDDAAHGKQVKIEDVAADKRRQFSINDAEVMELAKQAMVIEKHYGRPMDIEWAKDG
NDGKLYIVQARPETVRSREDVQLIERYHLKSRGAVISEGRAIGHKVGSGVAKVLTSIADMDQIQPGDVLVTDMTDPDWEP
IMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTDRIKNGQLVTVSCAEGDTGFIYEGKQEFEVISNRVDSLPALP
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQEEINEMIAGYESPVEFYIARLVEGI
ASIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENPMLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEV
MIPFVRTVKEAEQVVGLLAEQGLERGKDGLRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
DERDQAVKILLSMAIKAAKAKGAYIGICGQGPSDHADFAGWLVEQGIDTVSLNPDTVIDTWLYLAEAHG

Sequences:

>Translated_789_residues
MQQYVLWYQELGMGDVNLVGGKNASLGEMISNLANAGVQVPGGFATTSYAFNEFLEQSGVNQKIYDILATLDVDDVNALA
KVGAQIRQWVIDTPFQPALEQAIREAYDKLAAETSDASFAVRSSATAEDMPDASFAGQQETFLNVKGFDSVLVAIKHVFA
SLFNDRAISYRVHQGYEHHGVALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
PILSQGHKAVVRRNIGSKLIQMVYSDDAAHGKQVKIEDVAADKRRQFSINDAEVMELAKQAMVIEKHYGRPMDIEWAKDG
NDGKLYIVQARPETVRSREDVQLIERYHLKSRGAVISEGRAIGHKVGSGVAKVLTSIADMDQIQPGDVLVTDMTDPDWEP
IMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTDRIKNGQLVTVSCAEGDTGFIYEGKQEFEVISNRVDSLPALP
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQEEINEMIAGYESPVEFYIARLVEGI
ASIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENPMLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEV
MIPFVRTVKEAEQVVGLLAEQGLERGKDGLRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
DERDQAVKILLSMAIKAAKAKGAYIGICGQGPSDHADFAGWLVEQGIDTVSLNPDTVIDTWLYLAEAHG
>Mature_789_residues
MQQYVLWYQELGMGDVNLVGGKNASLGEMISNLANAGVQVPGGFATTSYAFNEFLEQSGVNQKIYDILATLDVDDVNALA
KVGAQIRQWVIDTPFQPALEQAIREAYDKLAAETSDASFAVRSSATAEDMPDASFAGQQETFLNVKGFDSVLVAIKHVFA
SLFNDRAISYRVHQGYEHHGVALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
PILSQGHKAVVRRNIGSKLIQMVYSDDAAHGKQVKIEDVAADKRRQFSINDAEVMELAKQAMVIEKHYGRPMDIEWAKDG
NDGKLYIVQARPETVRSREDVQLIERYHLKSRGAVISEGRAIGHKVGSGVAKVLTSIADMDQIQPGDVLVTDMTDPDWEP
IMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTDRIKNGQLVTVSCAEGDTGFIYEGKQEFEVISNRVDSLPALP
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQEEINEMIAGYESPVEFYIARLVEGI
ASIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENPMLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEV
MIPFVRTVKEAEQVVGLLAEQGLERGKDGLRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
DERDQAVKILLSMAIKAAKAKGAYIGICGQGPSDHADFAGWLVEQGIDTVSLNPDTVIDTWLYLAEAHG

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=782, Percent_Identity=74.8081841432225, Blast_Score=1253, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=456, Percent_Identity=27.8508771929825, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788726, Length=190, Percent_Identity=32.6315789473684, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI48994992, Length=190, Percent_Identity=33.1578947368421, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1789193, Length=403, Percent_Identity=25.3101736972705, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17564524, Length=329, Percent_Identity=26.4437689969605, Blast_Score=93, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 86549; Mature: 86549

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYVLWYQELGMGDVNLVGGKNASLGEMISNLANAGVQVPGGFATTSYAFNEFLEQSGV
CCCCEEEHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
NQKIYDILATLDVDDVNALAKVGAQIRQWVIDTPFQPALEQAIREAYDKLAAETSDASFA
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
VRSSATAEDMPDASFAGQQETFLNVKGFDSVLVAIKHVFASLFNDRAISYRVHQGYEHHG
EECCCCCCCCCCCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
VALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCCCEEEEC
PILSQGHKAVVRRNIGSKLIQMVYSDDAAHGKQVKIEDVAADKRRQFSINDAEVMELAKQ
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCHHCCCCCHHHHHHHHHH
AMVIEKHYGRPMDIEWAKDGNDGKLYIVQARPETVRSREDVQLIERYHLKSRGAVISEGR
HHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHCCHHHHHHHHHHHHHHCCCEECCCC
AIGHKVGSGVAKVLTSIADMDQIQPGDVLVTDMTDPDWEPIMKRASAIVTNRGGRTCHAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHH
IIARELGVPAVVGCGDVTDRIKNGQLVTVSCAEGDTGFIYEGKQEFEVISNRVDSLPALP
HHHHHCCCCEEEECCHHHHHHCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHHCCCCCC
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQEEINE
HHHEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHH
MIAGYESPVEFYIARLVEGIASIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENP
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCCCCHHCCCCCCCCCCCCCC
MLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEVMIPFVRTVKEAEQVVGLLAE
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QGLERGKDGLRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
HHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHH
DERDQAVKILLSMAIKAAKAKGAYIGICGQGPSDHADFAGWLVEQGIDTVSLNPDTVIDT
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCHHHHH
WLYLAEAHG
HHHHHHCCC
>Mature Secondary Structure
MQQYVLWYQELGMGDVNLVGGKNASLGEMISNLANAGVQVPGGFATTSYAFNEFLEQSGV
CCCCEEEHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
NQKIYDILATLDVDDVNALAKVGAQIRQWVIDTPFQPALEQAIREAYDKLAAETSDASFA
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
VRSSATAEDMPDASFAGQQETFLNVKGFDSVLVAIKHVFASLFNDRAISYRVHQGYEHHG
EECCCCCCCCCCCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
VALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCCCEEEEC
PILSQGHKAVVRRNIGSKLIQMVYSDDAAHGKQVKIEDVAADKRRQFSINDAEVMELAKQ
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCHHCCCCCHHHHHHHHHH
AMVIEKHYGRPMDIEWAKDGNDGKLYIVQARPETVRSREDVQLIERYHLKSRGAVISEGR
HHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHCCHHHHHHHHHHHHHHCCCEECCCC
AIGHKVGSGVAKVLTSIADMDQIQPGDVLVTDMTDPDWEPIMKRASAIVTNRGGRTCHAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHH
IIARELGVPAVVGCGDVTDRIKNGQLVTVSCAEGDTGFIYEGKQEFEVISNRVDSLPALP
HHHHHCCCCEEEECCHHHHHHCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHHCCCCCC
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQEEINE
HHHEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHH
MIAGYESPVEFYIARLVEGIASIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENP
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCCCCHHCCCCCCCCCCCCCC
MLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEVMIPFVRTVKEAEQVVGLLAE
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QGLERGKDGLRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
HHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHH
DERDQAVKILLSMAIKAAKAKGAYIGICGQGPSDHADFAGWLVEQGIDTVSLNPDTVIDT
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCHHHHH
WLYLAEAHG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]