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The map label for this gene is cysB

Identifier: 120598577

GI number: 120598577

Start: 2027603

End: 2028580

Strand: Direct

Name: cysB

Synonym: Sputw3181_1760

Alternate gene names: 120598577

Gene position: 2027603-2028580 (Clockwise)

Preceding gene: 120598575

Following gene: 120598582

Centisome position: 43.06

GC content: 42.94

Gene sequence:

>978_bases
ATGAAACTGCAACAGCTCAGGTATATTGCTGAGGTCGTAAAACACAATCTCAACGTGTCGGCAACCGCTGAAAATTTGTA
CACATCTCAACCTGGGATCAGTAAACAAGTTCGGATGCTGGAGGATGAGTTAGGGATCCAAATTTTTGGTCGTAGTGGTA
AGCATTTAACCCATGTTACCCCCGCAGGGCAGCAGGTAATTAATATCGCTAACGACATTTTAGGCAAAGTAGAAAGCATT
AAAAAAGTGTCTGAAGAATATACTAAGCCGGATCAAGGTGAGTTAAATATTGCGACGACAGATACTCAGGCCCGTTATGC
ATTGCCAACGATTATTCGCCAGTTTATTGATCGTTACCCCAAAGTGAACTTGCATATGCATCAAGGGACACCATCACAAA
TAAGTGAGCAAGCTGCACGAGGCGATGCGGATTTTGCGATAGCAACAGAAGCGATGCACTTGTATTCCGATCTGATTATG
TTGCCTTGTTACCATTGGAATCGTTCAGTAGTCGTGCCAAAAGAGCATCCTTTGGCTACGCGTACTCAAAAAATGACGAT
TGAAGACCTAGCACGGTATCCTTTAGTCACCTATGTATTTGGTTTTGACCGTGCTTCAGAAATAGAAAAGGCATTTAACC
GTGCCAATTTAGAGCCGCGAGTGGTTTTTAGCGCCACCAGTGCTGATGTGCTGAAAACTTATGTGCGATTAGGGTTAGGC
GTTGGAGTGATAGCGTCTATGGCTATTGATCCTAATATTGATAAAGACTTAGTTGCCATTGATGCGGGCCATTTATTTGC
TCATAGTACAACGAAAATAGGTTTTAGAAAGGGGAGCTTCTTACGTAGTTATATGTATGACTTTATTCAGCACTTTGCGC
CACATTTGACTCGCGATGTGGTTGAGAAAGCGGTTGCTCTGCGCGATCAGCAGCTTATCGATGAAATGTTTGCTGATAAA
AATCTACCAATGCGTTGA

Upstream 100 bases:

>100_bases
TTTAATTTTTACTATATACTATAACAATTCATTATAGCTGAGTGTGATTTAGGTGCTCAGTGTTCAACAATATTCAGCGT
ATTTGAGACAAGGATATAAA

Downstream 100 bases:

>100_bases
TATTTTCTCTTTTGGAGATGTAAAAAATGCCGCTGATATCAGCGGCATTTTTATGGATACGATTCTTAAATGGATTTAAA
CATTAAGATAAATCGACTTT

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKLQQLRYIAEVVKHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVTPAGQQVINIANDILGKVESI
KKVSEEYTKPDQGELNIATTDTQARYALPTIIRQFIDRYPKVNLHMHQGTPSQISEQAARGDADFAIATEAMHLYSDLIM
LPCYHWNRSVVVPKEHPLATRTQKMTIEDLARYPLVTYVFGFDRASEIEKAFNRANLEPRVVFSATSADVLKTYVRLGLG
VGVIASMAIDPNIDKDLVAIDAGHLFAHSTTKIGFRKGSFLRSYMYDFIQHFAPHLTRDVVEKAVALRDQQLIDEMFADK
NLPMR

Sequences:

>Translated_325_residues
MKLQQLRYIAEVVKHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVTPAGQQVINIANDILGKVESI
KKVSEEYTKPDQGELNIATTDTQARYALPTIIRQFIDRYPKVNLHMHQGTPSQISEQAARGDADFAIATEAMHLYSDLIM
LPCYHWNRSVVVPKEHPLATRTQKMTIEDLARYPLVTYVFGFDRASEIEKAFNRANLEPRVVFSATSADVLKTYVRLGLG
VGVIASMAIDPNIDKDLVAIDAGHLFAHSTTKIGFRKGSFLRSYMYDFIQHFAPHLTRDVVEKAVALRDQQLIDEMFADK
NLPMR
>Mature_325_residues
MKLQQLRYIAEVVKHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVTPAGQQVINIANDILGKVESI
KKVSEEYTKPDQGELNIATTDTQARYALPTIIRQFIDRYPKVNLHMHQGTPSQISEQAARGDADFAIATEAMHLYSDLIM
LPCYHWNRSVVVPKEHPLATRTQKMTIEDLARYPLVTYVFGFDRASEIEKAFNRANLEPRVVFSATSADVLKTYVRLGLG
VGVIASMAIDPNIDKDLVAIDAGHLFAHSTTKIGFRKGSFLRSYMYDFIQHFAPHLTRDVVEKAVALRDQQLIDEMFADK
NLPMR

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=323, Percent_Identity=70.8978328173375, Blast_Score=490, Evalue=1e-140,
Organism=Escherichia coli, GI1788296, Length=314, Percent_Identity=37.5796178343949, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1790399, Length=287, Percent_Identity=25.7839721254355, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI157672245, Length=249, Percent_Identity=28.5140562248996, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788887, Length=291, Percent_Identity=26.4604810996564, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789204, Length=227, Percent_Identity=23.3480176211454, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787879, Length=249, Percent_Identity=24.8995983935743, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790262, Length=188, Percent_Identity=25.531914893617, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=26.8456375838926, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36627; Mature: 36627

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLRYIAEVVKHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVT
CCHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEC
PAGQQVINIANDILGKVESIKKVSEEYTKPDQGELNIATTDTQARYALPTIIRQFIDRYP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
KVNLHMHQGTPSQISEQAARGDADFAIATEAMHLYSDLIMLPCYHWNRSVVVPKEHPLAT
CEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEECCCEEECCCCCCCCH
RTQKMTIEDLARYPLVTYVFGFDRASEIEKAFNRANLEPRVVFSATSADVLKTYVRLGLG
HHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCC
VGVIASMAIDPNIDKDLVAIDAGHLFAHSTTKIGFRKGSFLRSYMYDFIQHFAPHLTRDV
HHHHHHHHCCCCCCCCEEEEECCHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VEKAVALRDQQLIDEMFADKNLPMR
HHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLQQLRYIAEVVKHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVT
CCHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEC
PAGQQVINIANDILGKVESIKKVSEEYTKPDQGELNIATTDTQARYALPTIIRQFIDRYP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
KVNLHMHQGTPSQISEQAARGDADFAIATEAMHLYSDLIMLPCYHWNRSVVVPKEHPLAT
CEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEECCCEEECCCCCCCCH
RTQKMTIEDLARYPLVTYVFGFDRASEIEKAFNRANLEPRVVFSATSADVLKTYVRLGLG
HHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCC
VGVIASMAIDPNIDKDLVAIDAGHLFAHSTTKIGFRKGSFLRSYMYDFIQHFAPHLTRDV
HHHHHHHHCCCCCCCCEEEEECCHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VEKAVALRDQQLIDEMFADKNLPMR
HHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]