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The map label for this gene is cobB [H]

Identifier: 120598563

GI number: 120598563

Start: 2010310

End: 2011044

Strand: Direct

Name: cobB [H]

Synonym: Sputw3181_1746

Alternate gene names: 120598563

Gene position: 2010310-2011044 (Clockwise)

Preceding gene: 120598561

Following gene: 120598564

Centisome position: 42.7

GC content: 47.07

Gene sequence:

>735_bases
ATGTACCAACATATAGTGGTATTAACTGGCGCGGGGATCTCTGCTGAATCGGGATTGCGTACCTTTCGCGATCAAGATGG
TTTATGGGAAGAGCATCATATTGAAGACGTTGCTACACCAGAAGGCTATGAGCGTGATGCTGAGTTAGTGGAACGATTTT
ATAATAGCCGTTGGCAACAATTGCATAGTGGGGCAGTGGCTCCAAACCCCGCACATCAGGCTTTAGCAAAACTCGAAGCG
CAGTTTGTAGGGCAATTATTGATAGTAACGCAGAATATTGATGATTTGCATGAGCGAGCAGGCTCCCGCCGGTTGTTGCA
TATGCATGGCGAATTGTCTAAAGGTCGGTGCCCGCGTTCGCGTCAAACCTTTCTTTTGCGTGAGCCATTTGGTCCTGATA
ATTGCTGTACCTGCTGTATTCCGGCTCAGCGTTTACGTCCCCACGTGGTCTGGTTTGGTGAAATGCCATTAGGAATGGAT
CGAATTCATGATGCGCTTGATAGTTGCGATCTGTTTATTGCAATAGGAACCTCTGGCACAGTCTATCCCGCAGCAGGGTT
TGTGGATAGCGCGAATCATCATGGTGCTCAGACGGTTGAAGTGAATTTAATGGCTCCCGATAGACACAGTCAATTTCAAT
ATCACCTTACAGGCAAAGCGGGTGATATAGTGCCTAGGCTTGTTGATACCATTTTGGCAGGTAAAGTGATTGGTAGCGAC
TTGGTGACAATATGA

Upstream 100 bases:

>100_bases
AATTCAAGCATAGTTTTTGTAAGGTTATACCAAGGAATAATGTTCACGACCATAAAAGTCCGTGACAATAAGAATAATAA
AGAGAATAAAAGGAATCGGC

Downstream 100 bases:

>100_bases
GCCAAAAACTCGCGATTATTATGCGTGGTTTGCCTGGAAGCGGTAAATCTTATTGGGTTGAGCAGTATATTGCCAACTTA
CCTTTGGATATGGCTTTTCG

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MYQHIVVLTGAGISAESGLRTFRDQDGLWEEHHIEDVATPEGYERDAELVERFYNSRWQQLHSGAVAPNPAHQALAKLEA
QFVGQLLIVTQNIDDLHERAGSRRLLHMHGELSKGRCPRSRQTFLLREPFGPDNCCTCCIPAQRLRPHVVWFGEMPLGMD
RIHDALDSCDLFIAIGTSGTVYPAAGFVDSANHHGAQTVEVNLMAPDRHSQFQYHLTGKAGDIVPRLVDTILAGKVIGSD
LVTI

Sequences:

>Translated_244_residues
MYQHIVVLTGAGISAESGLRTFRDQDGLWEEHHIEDVATPEGYERDAELVERFYNSRWQQLHSGAVAPNPAHQALAKLEA
QFVGQLLIVTQNIDDLHERAGSRRLLHMHGELSKGRCPRSRQTFLLREPFGPDNCCTCCIPAQRLRPHVVWFGEMPLGMD
RIHDALDSCDLFIAIGTSGTVYPAAGFVDSANHHGAQTVEVNLMAPDRHSQFQYHLTGKAGDIVPRLVDTILAGKVIGSD
LVTI
>Mature_244_residues
MYQHIVVLTGAGISAESGLRTFRDQDGLWEEHHIEDVATPEGYERDAELVERFYNSRWQQLHSGAVAPNPAHQALAKLEA
QFVGQLLIVTQNIDDLHERAGSRRLLHMHGELSKGRCPRSRQTFLLREPFGPDNCCTCCIPAQRLRPHVVWFGEMPLGMD
RIHDALDSCDLFIAIGTSGTVYPAAGFVDSANHHGAQTVEVNLMAPDRHSQFQYHLTGKAGDIVPRLVDTILAGKVIGSD
LVTI

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=232, Percent_Identity=36.6379310344828, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI6912664, Length=253, Percent_Identity=33.596837944664, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI13787215, Length=242, Percent_Identity=33.4710743801653, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI6912662, Length=267, Percent_Identity=28.8389513108614, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI7657575, Length=249, Percent_Identity=28.1124497991968, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI308199517, Length=235, Percent_Identity=57.0212765957447, Blast_Score=266, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI17541892, Length=197, Percent_Identity=32.48730964467, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71990482, Length=247, Percent_Identity=28.3400809716599, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17137536, Length=229, Percent_Identity=29.2576419213974, Blast_Score=81, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27066; Mature: 27066

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQHIVVLTGAGISAESGLRTFRDQDGLWEEHHIEDVATPEGYERDAELVERFYNSRWQQ
CCCEEEEEECCCCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LHSGAVAPNPAHQALAKLEAQFVGQLLIVTQNIDDLHERAGSRRLLHMHGELSKGRCPRS
HHCCCCCCCHHHHHHHHHHHHHHCEEEEEECCHHHHHHCCCCCEEEEECCCCCCCCCCCC
RQTFLLREPFGPDNCCTCCIPAQRLRPHVVWFGEMPLGMDRIHDALDSCDLFIAIGTSGT
CCEEEEECCCCCCCCEEECCCHHHCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCC
VYPAAGFVDSANHHGAQTVEVNLMAPDRHSQFQYHLTGKAGDIVPRLVDTILAGKVIGSD
EEECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCC
LVTI
CCCC
>Mature Secondary Structure
MYQHIVVLTGAGISAESGLRTFRDQDGLWEEHHIEDVATPEGYERDAELVERFYNSRWQQ
CCCEEEEEECCCCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LHSGAVAPNPAHQALAKLEAQFVGQLLIVTQNIDDLHERAGSRRLLHMHGELSKGRCPRS
HHCCCCCCCHHHHHHHHHHHHHHCEEEEEECCHHHHHHCCCCCEEEEECCCCCCCCCCCC
RQTFLLREPFGPDNCCTCCIPAQRLRPHVVWFGEMPLGMDRIHDALDSCDLFIAIGTSGT
CCEEEEECCCCCCCCEEECCCHHHCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCC
VYPAAGFVDSANHHGAQTVEVNLMAPDRHSQFQYHLTGKAGDIVPRLVDTILAGKVIGSD
EEECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCC
LVTI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]