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The map label for this gene is sucB [H]

Identifier: 120598557

GI number: 120598557

Start: 2004837

End: 2006039

Strand: Direct

Name: sucB [H]

Synonym: Sputw3181_1740

Alternate gene names: 120598557

Gene position: 2004837-2006039 (Clockwise)

Preceding gene: 120598556

Following gene: 120598558

Centisome position: 42.58

GC content: 46.38

Gene sequence:

>1203_bases
ATGAGTATCGAAATCAAGGTACCCGTACTACCAGAATCTGTTGCCGATGCTACGATTGCCACTTGGCACGTTAAAGTGGG
TGAGCATGTTTCTCGTGATCAAAATCTTGTTGATATTGAAACAGATAAAGTTGTACTCGAAGTGGTTGCGCCTGAAGATG
GCCACATTGGTGAGTTTCTGTTCCAAGAGGGCGATACCGTACTTGGTGAACAAGTGATTGCTAAGTTCATCGCGGGGGCT
GTTGCTGGTCAAGAAGTCACTAAAGCCCAAGCTGAAGCGGCAGCACCGGTTGCTGAAACAAGTGATGAAAGTAATGATGC
GTTAAGTCCATCTGTACGCCGTTTACTGGCAGAGCACAATGTTGATGCTAGCAAAGTGAAAGGTACAGGTGTAGGCGGTC
GTATCACTAAAGAAGACGTTGAAGCTTTTGTTAGCTCTGCATCAAAAGGGGCGCCCAAAGCAGCGGCTCCTGCAGCACCT
GCCGTCGCGCCATTAGCAGAAGGCCGTAGTGAGAAACGTGTACCTATGACACGTTTACGTAAAACTATCGCTAACCGTCT
ATTAGAAGCGAAAAACTCTACCGCTATGCTCACGACGTTCAACGAAGTGAACATGAAGCCCATCATGGATATCCGTAAGC
AGTACCAAGATATCTTTGAAAAGCGCCACGGCATCCGTTTAGGTTTTATGTCTTTCTATGTGAAAGCAGTGACAGAAGCC
CTGAAACGCTTCCCAGAAGTGAACGCATCAATCGATGGTGATGATATTGTTTATCACAATTATTTCGATATTAGCATTGC
TGTATCAACACCACGTGGTCTGGTAACACCTGTCTTACGTGACACAGATACTATGAGTCTTGCTGAAATTGAAAAAGCAG
TACGCGATCTCGCTATCAAAGGCCGTGATGGCAAACTGACGGTTGCCGATATGACTGGTGGTAACTTCACCGTGACTAAC
GGCGGCGTTTTCGGTTCACTGATGTCTACACCGATCCTTAACCTGCCACAAAGTGCGATTTTAGGCATGCATGCCATTAA
AGATCGTCCAATGGCAGTCAATGGTCAGGTTGAAATCCTGCCCATGATGTACCTAGCACTCTCATATGACCATCGTATTG
TTGATGGCCGTGAGTCTGTTGGTTTCTTGGTTGCGATTAAAGACTTCCTAGAAGACCCAACACGTCTGCTGCTCGATCTA
TAA

Upstream 100 bases:

>100_bases
GCATGTGGTTACCCTGAGTTGCACGCACATCAGCAAGAATCTTTAGTGAACAGTGCATTGAAACTGTAGTAATAGACATT
TTATAAAAGGATCGTTTTTC

Downstream 100 bases:

>100_bases
GAAAAAGCCAGACATACCCTCGTGACTGGCCCTAAGGGCCAGTTGGATTTGATTAGAAGTATACGGATAGATCATCATGA
ATTTGCATGAGTATCAGGCA

Product: 2-oxoglutarate dehydrogenase, E2 subunit, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSIEIKVPVLPESVADATIATWHVKVGEHVSRDQNLVDIETDKVVLEVVAPEDGHIGEFLFQEGDTVLGEQVIAKFIAGA
VAGQEVTKAQAEAAAPVAETSDESNDALSPSVRRLLAEHNVDASKVKGTGVGGRITKEDVEAFVSSASKGAPKAAAPAAP
AVAPLAEGRSEKRVPMTRLRKTIANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKRHGIRLGFMSFYVKAVTEA
LKRFPEVNASIDGDDIVYHNYFDISIAVSTPRGLVTPVLRDTDTMSLAEIEKAVRDLAIKGRDGKLTVADMTGGNFTVTN
GGVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRIVDGRESVGFLVAIKDFLEDPTRLLLDL

Sequences:

>Translated_400_residues
MSIEIKVPVLPESVADATIATWHVKVGEHVSRDQNLVDIETDKVVLEVVAPEDGHIGEFLFQEGDTVLGEQVIAKFIAGA
VAGQEVTKAQAEAAAPVAETSDESNDALSPSVRRLLAEHNVDASKVKGTGVGGRITKEDVEAFVSSASKGAPKAAAPAAP
AVAPLAEGRSEKRVPMTRLRKTIANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKRHGIRLGFMSFYVKAVTEA
LKRFPEVNASIDGDDIVYHNYFDISIAVSTPRGLVTPVLRDTDTMSLAEIEKAVRDLAIKGRDGKLTVADMTGGNFTVTN
GGVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRIVDGRESVGFLVAIKDFLEDPTRLLLDL
>Mature_399_residues
SIEIKVPVLPESVADATIATWHVKVGEHVSRDQNLVDIETDKVVLEVVAPEDGHIGEFLFQEGDTVLGEQVIAKFIAGAV
AGQEVTKAQAEAAAPVAETSDESNDALSPSVRRLLAEHNVDASKVKGTGVGGRITKEDVEAFVSSASKGAPKAAAPAAPA
VAPLAEGRSEKRVPMTRLRKTIANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKRHGIRLGFMSFYVKAVTEAL
KRFPEVNASIDGDDIVYHNYFDISIAVSTPRGLVTPVLRDTDTMSLAEIEKAVRDLAIKGRDGKLTVADMTGGNFTVTNG
GVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRIVDGRESVGFLVAIKDFLEDPTRLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=250, Percent_Identity=55.2, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=31.0344827586207, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI110671329, Length=420, Percent_Identity=26.9047619047619, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI203098753, Length=452, Percent_Identity=28.0973451327434, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI203098816, Length=452, Percent_Identity=28.0973451327434, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=34.7826086956522, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1786946, Length=404, Percent_Identity=70.5445544554455, Blast_Score=569, Evalue=1e-164,
Organism=Escherichia coli, GI1786305, Length=407, Percent_Identity=33.1695331695332, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25146366, Length=409, Percent_Identity=43.0317848410758, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI17560088, Length=439, Percent_Identity=30.751708428246, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=26.1792452830189, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=316, Percent_Identity=29.746835443038, Blast_Score=131, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6320352, Length=404, Percent_Identity=43.0693069306931, Blast_Score=315, Evalue=9e-87,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=24.7216035634744, Blast_Score=132, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24645909, Length=225, Percent_Identity=60.8888888888889, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI18859875, Length=421, Percent_Identity=29.6912114014252, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=33.6244541484716, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=33.6244541484716, Blast_Score=131, Evalue=8e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43261; Mature: 43130

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEIKVPVLPESVADATIATWHVKVGEHVSRDQNLVDIETDKVVLEVVAPEDGHIGEFL
CEEEEEECCCCCHHCCCEEEEEEEECCCCCCCCCCEEEEECCEEEEEEECCCCCCHHHHH
FQEGDTVLGEQVIAKFIAGAVAGQEVTKAQAEAAAPVAETSDESNDALSPSVRRLLAEHN
HHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCC
VDASKVKGTGVGGRITKEDVEAFVSSASKGAPKAAAPAAPAVAPLAEGRSEKRVPMTRLR
CCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHH
KTIANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKRHGIRLGFMSFYVKAVTEA
HHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHH
LKRFPEVNASIDGDDIVYHNYFDISIAVSTPRGLVTPVLRDTDTMSLAEIEKAVRDLAIK
HHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHEEE
GRDGKLTVADMTGGNFTVTNGGVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEIL
CCCCEEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCEEHH
PMMYLALSYDHRIVDGRESVGFLVAIKDFLEDPTRLLLDL
HHHHHHHCCCCEEECCCCCCCHHEEHHHHHCCHHHHHHCC
>Mature Secondary Structure 
SIEIKVPVLPESVADATIATWHVKVGEHVSRDQNLVDIETDKVVLEVVAPEDGHIGEFL
EEEEEECCCCCHHCCCEEEEEEEECCCCCCCCCCEEEEECCEEEEEEECCCCCCHHHHH
FQEGDTVLGEQVIAKFIAGAVAGQEVTKAQAEAAAPVAETSDESNDALSPSVRRLLAEHN
HHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCC
VDASKVKGTGVGGRITKEDVEAFVSSASKGAPKAAAPAAPAVAPLAEGRSEKRVPMTRLR
CCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHH
KTIANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKRHGIRLGFMSFYVKAVTEA
HHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHH
LKRFPEVNASIDGDDIVYHNYFDISIAVSTPRGLVTPVLRDTDTMSLAEIEKAVRDLAIK
HHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHEEE
GRDGKLTVADMTGGNFTVTNGGVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEIL
CCCCEEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCEEHH
PMMYLALSYDHRIVDGRESVGFLVAIKDFLEDPTRLLLDL
HHHHHHHCCCCEEECCCCCCCHHEEHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]