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The map label for this gene is bioB [H]

Identifier: 120598480

GI number: 120598480

Start: 1916810

End: 1917862

Strand: Direct

Name: bioB [H]

Synonym: Sputw3181_1663

Alternate gene names: 120598480

Gene position: 1916810-1917862 (Clockwise)

Preceding gene: 120598477

Following gene: 120598481

Centisome position: 40.71

GC content: 48.43

Gene sequence:

>1053_bases
ATGTCGCAGTTGCAAGTTCGCCATGATTGGAAGCGGGAAGAGATCGAAGCCTTATTTGCGCTACCGATGAATGACTTATT
ATTTAAAGCCCACAGTATTCACCGTGAGGTGTACGATCCTAATGAAGTGCAGATCAGCCGTTTGCTATCAATTAAAACGG
GGGCTTGTCCTGAGGATTGTAAATATTGCCCGCAGAGTGCTCGCTATGATACAGGTCTAGAGAAAGAGCGTTTGCTGGCG
ATGGAAACCGTGTTGACCGAGGCACGCAGTGCGAAAGCCGCGGGAGCTTCCCGTTTCTGCATGGGTGCAGCTTGGCGTAA
CCCCAAAGAAAAAGACATGCCATACCTTAAGCAAATGGTACAAGAGGTCAAAGCCTTAGGTATGGAAACCTGCATGACGC
TTGGTATGTTATCCGCTGAACAAGCGAATGAGTTAGCAGATGCTGGACTAGATTATTACAACCACAATTTAGACACTTCG
CCTGAATATTACGGTGATGTGATCACGACGCGTACCTATCAAAATCGTTTAGATACATTAACTCATGTACGCGCATCTGG
GATGAAAGTGTGCTCAGGTGGAATTGTCGGTATGGGTGAAAAGGCAACTGATCGCGCAGGCTTACTGCAACAACTGGCTA
ATTTACCGCAACATCCTGATTCAGTGCCTATCAATATGTTAGTCAAAGTGGCGGGGACGCCGTTTGAGAAGCTTGACGAT
CTCGACCCACTCGAATTCGTGCGTACTATCGCCGTGGCGCGTATCTTAATGCCACTTTCTCGCGTGCGTTTATCGGCGGG
TCGCGAAAATATGAGTGATGAACTGCAGGCGATGTGTTTCTTTGCTGGTGCCAATTCGATTTTCTATGGTTGCAAATTGC
TAACCACACCTAACCCAGAAGAAAGCGATGATATGGGGTTATTCCGTCGTTTAGGTTTGCGTCCTGAGCAGGGCGCCGTG
GCAACCCTTGATGATGAACAGGCGGTGCTGGCTAAAGCGGCTGCCCATCAAGATAAATCAACAGCGCAGTTCTATGATGC
TGCGGCGCTGTAA

Upstream 100 bases:

>100_bases
AAGTAATCAGTAGTAAACTTGCCTTACTGGTTGGCGTTGACAGTTTAAGGGCTGAGGTTATCCTAGCCAAGATAAAAGAC
AGAATAATAAAAGGTGTGTA

Downstream 100 bases:

>100_bases
TTAAATGAGTTTTCAGCTCGATGCGAAAATAGCTGCTCGTCAACAGGCTTTGTTTGAGCAAGGACTGCTGAGACAACGTA
ACGCCTTATGCACAGACAGT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSQLQVRHDWKREEIEALFALPMNDLLFKAHSIHREVYDPNEVQISRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLA
METVLTEARSAKAAGASRFCMGAAWRNPKEKDMPYLKQMVQEVKALGMETCMTLGMLSAEQANELADAGLDYYNHNLDTS
PEYYGDVITTRTYQNRLDTLTHVRASGMKVCSGGIVGMGEKATDRAGLLQQLANLPQHPDSVPINMLVKVAGTPFEKLDD
LDPLEFVRTIAVARILMPLSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPEESDDMGLFRRLGLRPEQGAV
ATLDDEQAVLAKAAAHQDKSTAQFYDAAAL

Sequences:

>Translated_350_residues
MSQLQVRHDWKREEIEALFALPMNDLLFKAHSIHREVYDPNEVQISRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLA
METVLTEARSAKAAGASRFCMGAAWRNPKEKDMPYLKQMVQEVKALGMETCMTLGMLSAEQANELADAGLDYYNHNLDTS
PEYYGDVITTRTYQNRLDTLTHVRASGMKVCSGGIVGMGEKATDRAGLLQQLANLPQHPDSVPINMLVKVAGTPFEKLDD
LDPLEFVRTIAVARILMPLSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPEESDDMGLFRRLGLRPEQGAV
ATLDDEQAVLAKAAAHQDKSTAQFYDAAAL
>Mature_349_residues
SQLQVRHDWKREEIEALFALPMNDLLFKAHSIHREVYDPNEVQISRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLAM
ETVLTEARSAKAAGASRFCMGAAWRNPKEKDMPYLKQMVQEVKALGMETCMTLGMLSAEQANELADAGLDYYNHNLDTSP
EYYGDVITTRTYQNRLDTLTHVRASGMKVCSGGIVGMGEKATDRAGLLQQLANLPQHPDSVPINMLVKVAGTPFEKLDDL
DPLEFVRTIAVARILMPLSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPEESDDMGLFRRLGLRPEQGAVA
TLDDEQAVLAKAAAHQDKSTAQFYDAAAL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=345, Percent_Identity=67.2463768115942, Blast_Score=487, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6321725, Length=316, Percent_Identity=46.5189873417722, Blast_Score=310, Evalue=3e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 38821; Mature: 38690

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLQVRHDWKREEIEALFALPMNDLLFKAHSIHREVYDPNEVQISRLLSIKTGACPEDC
CCCCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
KYCPQSARYDTGLEKERLLAMETVLTEARSAKAAGASRFCMGAAWRNPKEKDMPYLKQMV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHH
QEVKALGMETCMTLGMLSAEQANELADAGLDYYNHNLDTSPEYYGDVITTRTYQNRLDTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHH
THVRASGMKVCSGGIVGMGEKATDRAGLLQQLANLPQHPDSVPINMLVKVAGTPFEKLDD
HHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHCC
LDPLEFVRTIAVARILMPLSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCC
ESDDMGLFRRLGLRPEQGAVATLDDEQAVLAKAAAHQDKSTAQFYDAAAL
CCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
SQLQVRHDWKREEIEALFALPMNDLLFKAHSIHREVYDPNEVQISRLLSIKTGACPEDC
CCCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
KYCPQSARYDTGLEKERLLAMETVLTEARSAKAAGASRFCMGAAWRNPKEKDMPYLKQMV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHH
QEVKALGMETCMTLGMLSAEQANELADAGLDYYNHNLDTSPEYYGDVITTRTYQNRLDTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHH
THVRASGMKVCSGGIVGMGEKATDRAGLLQQLANLPQHPDSVPINMLVKVAGTPFEKLDD
HHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHCC
LDPLEFVRTIAVARILMPLSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCC
ESDDMGLFRRLGLRPEQGAVATLDDEQAVLAKAAAHQDKSTAQFYDAAAL
CCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA