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The map label for this gene is dsbA [H]

Identifier: 120598466

GI number: 120598466

Start: 1890284

End: 1890907

Strand: Direct

Name: dsbA [H]

Synonym: Sputw3181_1649

Alternate gene names: 120598466

Gene position: 1890284-1890907 (Clockwise)

Preceding gene: 120598465

Following gene: 120598467

Centisome position: 40.15

GC content: 43.27

Gene sequence:

>624_bases
ATGAGCACGTTAAATATTATTGTTAGACCACTGATGTCCCTTGCCTTCGGGTTCATACTCAGTGCCAGTTTTACCTTACA
GGCGGCTAATTTTGAGGAAGGTAAAGATTATGTAACAGTGACGGGGATTTCTGAGGCACAACAGCCAGTGTTAAGAGAGT
TTTTTTCTTATAACTGTCCGCATTGCTATAACCAAGAGCCCTTAATTGAATCGACCGTTACTTTACTCGGTAAAGAGATC
CATTTTGAGCGTACTCCCGTTGGTGCCGGTCGTCCAAGTTGGCAATTGAGCCAGTTAGCCTATTATGTGGCACAAAAACT
GAACATGACAAAGCAAACCCATGGTGCCATTTTTAAACAAATCCAAGTGAATGCAGAGCAATTTACTCGCCCAGAACAAG
TGAAGGCTTTTTTTGTCTCCCAAGGTGCTAAAGCTGAGGATGTAGAGGCTACAATCAATTCAGTTGATGCCCAATTCACG
CTAATGAACTACGACTCTCAAGCTGAGCTTGCAGGTATTAAAGGCGTCCCTTCATTATTGGTTAATGGCCGTTACATGTT
GACGTCAACCGCGCATACACCTGAAGAATTGGCGGCGTTAGTTAAGTTTTTAGCGGCGAAATAA

Upstream 100 bases:

>100_bases
TTTTAAGAGATAAGCGGGCACTTAGGTGGTTTTTATGGCCTAGGATTGGCTCGGTAAATGGTTTTTTTTAATCAAATGAC
AAAATAAAAGAGATAGTATA

Downstream 100 bases:

>100_bases
CGACATTTCATAGATGGATGGTATGCGATGAAAGCGGCTTTTTGCGAGTATCGTATAGTCACGTTTCCTCAAGGTAGATT
GAATAACCCAGCATAGACTG

Product: DSBA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSTLNIIVRPLMSLAFGFILSASFTLQAANFEEGKDYVTVTGISEAQQPVLREFFSYNCPHCYNQEPLIESTVTLLGKEI
HFERTPVGAGRPSWQLSQLAYYVAQKLNMTKQTHGAIFKQIQVNAEQFTRPEQVKAFFVSQGAKAEDVEATINSVDAQFT
LMNYDSQAELAGIKGVPSLLVNGRYMLTSTAHTPEELAALVKFLAAK

Sequences:

>Translated_207_residues
MSTLNIIVRPLMSLAFGFILSASFTLQAANFEEGKDYVTVTGISEAQQPVLREFFSYNCPHCYNQEPLIESTVTLLGKEI
HFERTPVGAGRPSWQLSQLAYYVAQKLNMTKQTHGAIFKQIQVNAEQFTRPEQVKAFFVSQGAKAEDVEATINSVDAQFT
LMNYDSQAELAGIKGVPSLLVNGRYMLTSTAHTPEELAALVKFLAAK
>Mature_206_residues
STLNIIVRPLMSLAFGFILSASFTLQAANFEEGKDYVTVTGISEAQQPVLREFFSYNCPHCYNQEPLIESTVTLLGKEIH
FERTPVGAGRPSWQLSQLAYYVAQKLNMTKQTHGAIFKQIQVNAEQFTRPEQVKAFFVSQGAKAEDVEATINSVDAQFTL
MNYDSQAELAGIKGVPSLLVNGRYMLTSTAHTPEELAALVKFLAAK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=203, Percent_Identity=26.6009852216749, Blast_Score=67, Evalue=6e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 22918; Mature: 22787

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLNIIVRPLMSLAFGFILSASFTLQAANFEEGKDYVTVTGISEAQQPVLREFFSYNCP
CCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCCCC
HCYNQEPLIESTVTLLGKEIHFERTPVGAGRPSWQLSQLAYYVAQKLNMTKQTHGAIFKQ
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
IQVNAEQFTRPEQVKAFFVSQGAKAEDVEATINSVDAQFTLMNYDSQAELAGIKGVPSLL
HCCCHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHCCCCCCHHHH
VNGRYMLTSTAHTPEELAALVKFLAAK
HCCEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
STLNIIVRPLMSLAFGFILSASFTLQAANFEEGKDYVTVTGISEAQQPVLREFFSYNCP
CHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCCCC
HCYNQEPLIESTVTLLGKEIHFERTPVGAGRPSWQLSQLAYYVAQKLNMTKQTHGAIFKQ
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
IQVNAEQFTRPEQVKAFFVSQGAKAEDVEATINSVDAQFTLMNYDSQAELAGIKGVPSLL
HCCCHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHCCCCCCHHHH
VNGRYMLTSTAHTPEELAALVKFLAAK
HCCEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA