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The map label for this gene is rlmI [H]

Identifier: 120598440

GI number: 120598440

Start: 1850977

End: 1851891

Strand: Direct

Name: rlmI [H]

Synonym: Sputw3181_1623

Alternate gene names: 120598440

Gene position: 1850977-1851891 (Clockwise)

Preceding gene: 120598439

Following gene: 120598441

Centisome position: 39.31

GC content: 44.48

Gene sequence:

>915_bases
ATGCAACGTTTTTTACAAGCGCTCACCACGTTAGAACTCGGTAATGATGCGGTACGTTTATTTCATGGTCGAGGCGGGCA
TTATGCTGGCTCTGAGCATATTTGTTTAGATTGGTTTGCCCCCGTTTTATTGTTAACCAGTTTTATTCCTCTCGAAGATA
CCGAGCTTGCGATGTGCCAGCAGGCCATTACAGCACGTTGGCAAGCATGGCATCCTGAAAGTCCTCTAAATCTCGTTTAT
CAATTTCGTTGTGCGGGGGAGACGTTTACTCAGTTACTTGAGGGCGAAATTCCAGAACCTCATATCGTTACTGAAAAGGG
AGCGCGATTTCAGGTGCATTTAATGCGCGGCCAAAATCATGGACTTTTTCTCGATATGGCCAACGGTCGAGATTGGGTGA
GGGCAAATGCACGCCATAAAAAGGTGCTTAATTTATTTGCTTATACCTGTGGATTCTCAGTTGCAGCACTGCAGGGCGGG
GCTGATGAAGTTGTGAATATGGATATGAGTAAAGGAGCCCTTGCTATTGGTAAACAAAACCATTTGCTGAATGGCTTTGT
CGCTGGAGCACGTTTTTTAGGCCATGATATTTTCAAATCTTGGGGCAAACTTAAAAAGCTCGGTCCCTATGAATTAATTG
TTGCCGATCCTCCGAGCAATCAGAAGGGGAGTTTTGTTGCTACTAAGGATTATGTGCGTTTAATTAGGCGGTTACCTGAA
TTACTTACCGATAATGGTGAAGTGTTACTGTGTTTAAATGCCCCTGAACTGGATACCGCTTTTCTGCGCAAACAGGTTGC
TGAAGCTGCGCCAGCATTGGAATATGTTGAGCGACTCGCTAATCCGCCAGCCTTTATCGATGTATCGGAAGAAAAGTCAT
TAAAAGTATTACGCTATCGAGTGAAGACTAACTGA

Upstream 100 bases:

>100_bases
TCGGATGATGTCGATGATAGACTCGTCAAAAGTACAGTGATATCTGAGCCTGTTAATCATCAGGCGACTCCTAGTTCAAC
AGAAAAGAAGTAAATTAATA

Downstream 100 bases:

>100_bases
AAGGTTTTCACTTCGCGATTCACTTTTGCCGCGTTAAAAGTGGATTTTGGTATTTTTTCGTTATTAATGGCAAACAAAAC
TGAAAGTTTTCTGTAACTGG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MQRFLQALTTLELGNDAVRLFHGRGGHYAGSEHICLDWFAPVLLLTSFIPLEDTELAMCQQAITARWQAWHPESPLNLVY
QFRCAGETFTQLLEGEIPEPHIVTEKGARFQVHLMRGQNHGLFLDMANGRDWVRANARHKKVLNLFAYTCGFSVAALQGG
ADEVVNMDMSKGALAIGKQNHLLNGFVAGARFLGHDIFKSWGKLKKLGPYELIVADPPSNQKGSFVATKDYVRLIRRLPE
LLTDNGEVLLCLNAPELDTAFLRKQVAEAAPALEYVERLANPPAFIDVSEEKSLKVLRYRVKTN

Sequences:

>Translated_304_residues
MQRFLQALTTLELGNDAVRLFHGRGGHYAGSEHICLDWFAPVLLLTSFIPLEDTELAMCQQAITARWQAWHPESPLNLVY
QFRCAGETFTQLLEGEIPEPHIVTEKGARFQVHLMRGQNHGLFLDMANGRDWVRANARHKKVLNLFAYTCGFSVAALQGG
ADEVVNMDMSKGALAIGKQNHLLNGFVAGARFLGHDIFKSWGKLKKLGPYELIVADPPSNQKGSFVATKDYVRLIRRLPE
LLTDNGEVLLCLNAPELDTAFLRKQVAEAAPALEYVERLANPPAFIDVSEEKSLKVLRYRVKTN
>Mature_304_residues
MQRFLQALTTLELGNDAVRLFHGRGGHYAGSEHICLDWFAPVLLLTSFIPLEDTELAMCQQAITARWQAWHPESPLNLVY
QFRCAGETFTQLLEGEIPEPHIVTEKGARFQVHLMRGQNHGLFLDMANGRDWVRANARHKKVLNLFAYTCGFSVAALQGG
ADEVVNMDMSKGALAIGKQNHLLNGFVAGARFLGHDIFKSWGKLKKLGPYELIVADPPSNQKGSFVATKDYVRLIRRLPE
LLTDNGEVLLCLNAPELDTAFLRKQVAEAAPALEYVERLANPPAFIDVSEEKSLKVLRYRVKTN

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=268, Percent_Identity=30.9701492537313, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787181, Length=164, Percent_Identity=32.9268292682927, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 34011; Mature: 34011

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRFLQALTTLELGNDAVRLFHGRGGHYAGSEHICLDWFAPVLLLTSFIPLEDTELAMCQ
CHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHH
QAITARWQAWHPESPLNLVYQFRCAGETFTQLLEGEIPEPHIVTEKGARFQVHLMRGQNH
HHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHCCCCCCCEEEECCCCEEEEEEEECCCC
GLFLDMANGRDWVRANARHKKVLNLFAYTCGFSVAALQGGADEVVNMDMSKGALAIGKQN
CEEEEECCCCCHHHCCHHHHHHHHHHHHHHCCCCEECCCCHHHHHCCCCCCCEEEECCCC
HLLNGFVAGARFLGHDIFKSWGKLKKLGPYELIVADPPSNQKGSFVATKDYVRLIRRLPE
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHH
LLTDNGEVLLCLNAPELDTAFLRKQVAEAAPALEYVERLANPPAFIDVSEEKSLKVLRYR
HHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEE
VKTN
ECCC
>Mature Secondary Structure
MQRFLQALTTLELGNDAVRLFHGRGGHYAGSEHICLDWFAPVLLLTSFIPLEDTELAMCQ
CHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHH
QAITARWQAWHPESPLNLVYQFRCAGETFTQLLEGEIPEPHIVTEKGARFQVHLMRGQNH
HHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHCCCCCCCEEEECCCCEEEEEEEECCCC
GLFLDMANGRDWVRANARHKKVLNLFAYTCGFSVAALQGGADEVVNMDMSKGALAIGKQN
CEEEEECCCCCHHHCCHHHHHHHHHHHHHHCCCCEECCCCHHHHHCCCCCCCEEEECCCC
HLLNGFVAGARFLGHDIFKSWGKLKKLGPYELIVADPPSNQKGSFVATKDYVRLIRRLPE
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHH
LLTDNGEVLLCLNAPELDTAFLRKQVAEAAPALEYVERLANPPAFIDVSEEKSLKVLRYR
HHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEE
VKTN
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA