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The map label for this gene is rluB [H]

Identifier: 120598427

GI number: 120598427

Start: 1834786

End: 1835661

Strand: Direct

Name: rluB [H]

Synonym: Sputw3181_1610

Alternate gene names: 120598427

Gene position: 1834786-1835661 (Clockwise)

Preceding gene: 120598426

Following gene: 120598428

Centisome position: 38.97

GC content: 50.68

Gene sequence:

>876_bases
ATGAGTGAAAAATTGCAGAAAGTCTTGGCCCGTGCAGGCCATGGCTCCCGTCGTGAGATGGAGGCATGGATTGCTGCAGG
CCGAGTTAGTATTGATGGTGAAATTGCTAGCCTTGGGGATCGTATTGAAGCCGATGCTAAAGTGCGTATCGATGGCAGAG
CCATTTCGTTAAAGTCTGCCGAAGACGTGATTTGCCGTGTACTGGCATATCATAAACCCGAAGGCGAAATTTGCAGTCGT
AAAGATCCTGAAGGCCGTCCAACGGTATTCGATCGCTTACCTAAAGTGCGTGATTCACGCTGGGTAGCGGTAGGTCGTTT
AGACATCAATACCTCAGGTTTATTGTTGTTTACCTCAGATGGTGAGTTAGCAAACCGCTTAATGCATCCATCGAACGAAG
TTGACCGCGAATACGCAGTGCGTACCTTCGGTGAAATCCCTGAGGCGACAGTGCAACGTTTGCGTACAGGTGTGATGTTA
GAGGACGGCCCAGCACAGTTTGATAGCATCAAGCCAGCGGGCGGCGAAGGCATGAACCAGTGGTGGCATGTGTGTTTACG
CGAAGGTCGTAACCGTGAAGTACGCCGTCTGTGGGAATCCCAAGAAGTGCAGGTGAGCCGTTTGATCCGTATTCGTTATG
GTATGGTCGAATTGCCAAAATCACTGCCCCGCGGTGGTTGGGTTGAATTGCCCGTTGAACAAGTTAATTATTTGCGTCAA
TTAGCGGGTCTTGAGCCTGAAACGCGCACTATGCTTGCTGCTGATAAACACAGTGTGGCCCGTGCTCAGGTTAAAAGTGC
CAAAATTCGCCGCGCGGTGCGTAAGCATAAAGTCACAGGGGCTGGAAAAGCTAAACCAACGCGCCAGCGCAGTTAA

Upstream 100 bases:

>100_bases
ACCTTAGCGGATTTACCGCCGCTGTCGGATACTGAGTCATTGCAGGCGGTTTTTTCAGGTTTGAAATCAGCGCCAGAACA
GTCAGAAGAGCAAAATACTA

Downstream 100 bases:

>100_bases
GTTGTTCTATCCCAATCAAAAGGCCGCTTCGCGGCCTTTTTGCTTTCTGTTATTTGCAGCTATGTTATAACAAGTCTCAG
TTGGATTGAGGAGTGAATGG

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSEKLQKVLARAGHGSRREMEAWIAAGRVSIDGEIASLGDRIEADAKVRIDGRAISLKSAEDVICRVLAYHKPEGEICSR
KDPEGRPTVFDRLPKVRDSRWVAVGRLDINTSGLLLFTSDGELANRLMHPSNEVDREYAVRTFGEIPEATVQRLRTGVML
EDGPAQFDSIKPAGGEGMNQWWHVCLREGRNREVRRLWESQEVQVSRLIRIRYGMVELPKSLPRGGWVELPVEQVNYLRQ
LAGLEPETRTMLAADKHSVARAQVKSAKIRRAVRKHKVTGAGKAKPTRQRS

Sequences:

>Translated_291_residues
MSEKLQKVLARAGHGSRREMEAWIAAGRVSIDGEIASLGDRIEADAKVRIDGRAISLKSAEDVICRVLAYHKPEGEICSR
KDPEGRPTVFDRLPKVRDSRWVAVGRLDINTSGLLLFTSDGELANRLMHPSNEVDREYAVRTFGEIPEATVQRLRTGVML
EDGPAQFDSIKPAGGEGMNQWWHVCLREGRNREVRRLWESQEVQVSRLIRIRYGMVELPKSLPRGGWVELPVEQVNYLRQ
LAGLEPETRTMLAADKHSVARAQVKSAKIRRAVRKHKVTGAGKAKPTRQRS
>Mature_290_residues
SEKLQKVLARAGHGSRREMEAWIAAGRVSIDGEIASLGDRIEADAKVRIDGRAISLKSAEDVICRVLAYHKPEGEICSRK
DPEGRPTVFDRLPKVRDSRWVAVGRLDINTSGLLLFTSDGELANRLMHPSNEVDREYAVRTFGEIPEATVQRLRTGVMLE
DGPAQFDSIKPAGGEGMNQWWHVCLREGRNREVRRLWESQEVQVSRLIRIRYGMVELPKSLPRGGWVELPVEQVNYLRQL
AGLEPETRTMLAADKHSVARAQVKSAKIRRAVRKHKVTGAGKAKPTRQRS

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=285, Percent_Identity=65.2631578947368, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1790453, Length=241, Percent_Identity=29.045643153527, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=29.0598290598291, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081838, Length=202, Percent_Identity=29.7029702970297, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 32743; Mature: 32612

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLQKVLARAGHGSRREMEAWIAAGRVSIDGEIASLGDRIEADAKVRIDGRAISLKSA
CCHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCHHHHHCCCCCCCCEEEECCEEEECCCH
EDVICRVLAYHKPEGEICSRKDPEGRPTVFDRLPKVRDSRWVAVGRLDINTSGLLLFTSD
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEEECCCEEEEEECC
GELANRLMHPSNEVDREYAVRTFGEIPEATVQRLRTGVMLEDGPAQFDSIKPAGGEGMNQ
CHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCEECCCCCCCCCCCCCCCCCHHH
WWHVCLREGRNREVRRLWESQEVQVSRLIRIRYGMVELPKSLPRGGWVELPVEQVNYLRQ
HHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHH
LAGLEPETRTMLAADKHSVARAQVKSAKIRRAVRKHKVTGAGKAKPTRQRS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEKLQKVLARAGHGSRREMEAWIAAGRVSIDGEIASLGDRIEADAKVRIDGRAISLKSA
CHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCHHHHHCCCCCCCCEEEECCEEEECCCH
EDVICRVLAYHKPEGEICSRKDPEGRPTVFDRLPKVRDSRWVAVGRLDINTSGLLLFTSD
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEEECCCEEEEEECC
GELANRLMHPSNEVDREYAVRTFGEIPEATVQRLRTGVMLEDGPAQFDSIKPAGGEGMNQ
CHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCEECCCCCCCCCCCCCCCCCHHH
WWHVCLREGRNREVRRLWESQEVQVSRLIRIRYGMVELPKSLPRGGWVELPVEQVNYLRQ
HHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHH
LAGLEPETRTMLAADKHSVARAQVKSAKIRRAVRKHKVTGAGKAKPTRQRS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]