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The map label for this gene is hcaT [H]

Identifier: 120598376

GI number: 120598376

Start: 1774306

End: 1775481

Strand: Direct

Name: hcaT [H]

Synonym: Sputw3181_1558

Alternate gene names: 120598376

Gene position: 1774306-1775481 (Clockwise)

Preceding gene: 120598375

Following gene: 120598377

Centisome position: 37.68

GC content: 51.11

Gene sequence:

>1176_bases
ATGTCAACTTCTTCTAGCACAAGCCGGCAATTGCGCTGGCTTTGTGCCTGCTATTTTTTCTTCTTTTCTATTCTCGGCAC
TATGGTCCCTTATCTTGGGGTCTTCTTCGAAAATCGCGGTTTTAATCCCCAAGAAATTGGCTTTTTACTCGCCATCCTGA
TGGCGACGCGCATCGTTGCACCTAATGTGTGGGCTAAAGTGGCTGACCGAACGGGGATGCGCGCCGAGCTGATCAAAATG
GGGGCGGGAGCCGCCGCGCTCGCGTATTTGAGTTTCTTCTACCATGGTGGTTTTGTGTATATGGCGCTCAGTTTGGCCCT
CTATACTTTCTTTTGGAATGCGATCCTCGCCCAGCTTGAAGTGATCACCTTAGAAACCTTAGGCGAGAATGCCAGCCGCT
ACGGTCAAATACGCAGTTTTGGCAGCATAGGTTTTATCTGCCTTGTGGTGGGTGCGGGCTTTGCTATCGGCCAGTGGGGC
ACCGAAGTGTTGCCTTATATCGGCTTGATGTTATTTACTGGGATGCTGCTTAGCGCGTTGCCGCTACCCGCCAATCGAGC
GGTGCGCCCCGCGGGGCAGGAGCGGCACCGCCTTAAATGGACTAAGCCAATTGTGTGGTTCATGATTTCCGCTATGTTGT
TGCAAATGAGCGCAGGGCCTTTCTACGGCTTCTTCGTCTTATATTTAAAGCAAGCGGGTTACTCGGAATCCTCGGCGGGC
ATATTTGTCGCCCTTGGCGCTATGGCTGAAATTGTGATGTTTATGTTCGCGCCGCGTTTACTCGGCCGCTATGGCGTGAA
TACCTTATTGATTGTCAGCATTGGCATGACTTGTCTACGTTGGTTATTGGTTGCCTTTGGGGTCGATAATGTGTTGTGGC
TAGGTTTTAGCCAATTATTACATGCCTTTACCTTTGGCTTAACCCACGCCGCATCGATTCAATTTGTCCATAAACATTTC
GATGCGAGCCACAGAAGCCAAGGTCAAGCCCTATACGCCAGTTTAAGTTTTGGGGTTGGCGGCGCGCTCGGTACTTGGAT
TTGTGGCTTTATTTGGGGCGATGGTAGCGGGGCGGTTTGGTCTTGGGTATTTGCCGCAGTATGCGCCTTTGCCGCTATGC
TGGCGGTATTCTTTATCCCTAAGGATAAACCGAGTGTTGCAGCCCATAAGGCCTAG

Upstream 100 bases:

>100_bases
CGTTCTCGGAATGAGATAGGGGTATGCGATATAGGTGGCATGAAATAGCAGGTATGCGATAACGATAAAATGAGTGGCAT
GAGATAAATGGAATGCGTTA

Downstream 100 bases:

>100_bases
TGATATCCATTAAGATAAAAATCAAAAGATCGTGTTAAGTGAGGTAAAGGGTGATTCGGTTTCGTTTAGGGTTAGTTATC
AATCCATTAGCTGGCTTAGG

Product: major facilitator superfamily transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSTSSSTSRQLRWLCACYFFFFSILGTMVPYLGVFFENRGFNPQEIGFLLAILMATRIVAPNVWAKVADRTGMRAELIKM
GAGAAALAYLSFFYHGGFVYMALSLALYTFFWNAILAQLEVITLETLGENASRYGQIRSFGSIGFICLVVGAGFAIGQWG
TEVLPYIGLMLFTGMLLSALPLPANRAVRPAGQERHRLKWTKPIVWFMISAMLLQMSAGPFYGFFVLYLKQAGYSESSAG
IFVALGAMAEIVMFMFAPRLLGRYGVNTLLIVSIGMTCLRWLLVAFGVDNVLWLGFSQLLHAFTFGLTHAASIQFVHKHF
DASHRSQGQALYASLSFGVGGALGTWICGFIWGDGSGAVWSWVFAAVCAFAAMLAVFFIPKDKPSVAAHKA

Sequences:

>Translated_391_residues
MSTSSSTSRQLRWLCACYFFFFSILGTMVPYLGVFFENRGFNPQEIGFLLAILMATRIVAPNVWAKVADRTGMRAELIKM
GAGAAALAYLSFFYHGGFVYMALSLALYTFFWNAILAQLEVITLETLGENASRYGQIRSFGSIGFICLVVGAGFAIGQWG
TEVLPYIGLMLFTGMLLSALPLPANRAVRPAGQERHRLKWTKPIVWFMISAMLLQMSAGPFYGFFVLYLKQAGYSESSAG
IFVALGAMAEIVMFMFAPRLLGRYGVNTLLIVSIGMTCLRWLLVAFGVDNVLWLGFSQLLHAFTFGLTHAASIQFVHKHF
DASHRSQGQALYASLSFGVGGALGTWICGFIWGDGSGAVWSWVFAAVCAFAAMLAVFFIPKDKPSVAAHKA
>Mature_390_residues
STSSSTSRQLRWLCACYFFFFSILGTMVPYLGVFFENRGFNPQEIGFLLAILMATRIVAPNVWAKVADRTGMRAELIKMG
AGAAALAYLSFFYHGGFVYMALSLALYTFFWNAILAQLEVITLETLGENASRYGQIRSFGSIGFICLVVGAGFAIGQWGT
EVLPYIGLMLFTGMLLSALPLPANRAVRPAGQERHRLKWTKPIVWFMISAMLLQMSAGPFYGFFVLYLKQAGYSESSAGI
FVALGAMAEIVMFMFAPRLLGRYGVNTLLIVSIGMTCLRWLLVAFGVDNVLWLGFSQLLHAFTFGLTHAASIQFVHKHFD
ASHRSQGQALYASLSFGVGGALGTWICGFIWGDGSGAVWSWVFAAVCAFAAMLAVFFIPKDKPSVAAHKA

Specific function: Probable permease involved in the uptake of 3- phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family. HcaT subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788886, Length=391, Percent_Identity=24.8081841432225, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR016196
- InterPro:   IPR005276 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 42841; Mature: 42710

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSSSTSRQLRWLCACYFFFFSILGTMVPYLGVFFENRGFNPQEIGFLLAILMATRIVA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
PNVWAKVADRTGMRAELIKMGAGAAALAYLSFFYHGGFVYMALSLALYTFFWNAILAQLE
CHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VITLETLGENASRYGQIRSFGSIGFICLVVGAGFAIGQWGTEVLPYIGLMLFTGMLLSAL
HHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PLPANRAVRPAGQERHRLKWTKPIVWFMISAMLLQMSAGPFYGFFVLYLKQAGYSESSAG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
IFVALGAMAEIVMFMFAPRLLGRYGVNTLLIVSIGMTCLRWLLVAFGVDNVLWLGFSQLL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
HAFTFGLTHAASIQFVHKHFDASHRSQGQALYASLSFGVGGALGTWICGFIWGDGSGAVW
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHEECCCCHHH
SWVFAAVCAFAAMLAVFFIPKDKPSVAAHKA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
STSSSTSRQLRWLCACYFFFFSILGTMVPYLGVFFENRGFNPQEIGFLLAILMATRIVA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
PNVWAKVADRTGMRAELIKMGAGAAALAYLSFFYHGGFVYMALSLALYTFFWNAILAQLE
CHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VITLETLGENASRYGQIRSFGSIGFICLVVGAGFAIGQWGTEVLPYIGLMLFTGMLLSAL
HHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PLPANRAVRPAGQERHRLKWTKPIVWFMISAMLLQMSAGPFYGFFVLYLKQAGYSESSAG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
IFVALGAMAEIVMFMFAPRLLGRYGVNTLLIVSIGMTCLRWLLVAFGVDNVLWLGFSQLL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
HAFTFGLTHAASIQFVHKHFDASHRSQGQALYASLSFGVGGALGTWICGFIWGDGSGAVW
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHEECCCCHHH
SWVFAAVCAFAAMLAVFFIPKDKPSVAAHKA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9603882; 9205837; 9278503 [H]