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The map label for this gene is gltX

Identifier: 120598327

GI number: 120598327

Start: 1711295

End: 1712704

Strand: Direct

Name: gltX

Synonym: Sputw3181_1509

Alternate gene names: 120598327

Gene position: 1711295-1712704 (Clockwise)

Preceding gene: 120598321

Following gene: 120598329

Centisome position: 36.35

GC content: 43.33

Gene sequence:

>1410_bases
ATGACAACTAAGACGCGTTTTGCCCCTAGTCCTACCGGTTTCTTGCATGTTGGTGGTGCCCGTACTGCACTTTATTCTTG
GTTACAAGCCCGTGCCAATAACGGTGAGTTTGTATTACGTATTGAAGATACGGATATTGAGCGTTCAACTCAAGCTGCTT
GCGATGCGATTTTAGAAGGCATGAACTGGCTAGGTTTGACATGGGACGAAGGCCCATATTACCAAACTAAGCGTTTTGAC
CGTTATAACGAAATCATTGCTCAAATGTTAGAAAAGGGCACAGCGTATAAATGTTACTGTTCACGTGAGCGAATCGATGC
CTTAAGAGAATCACAGGCCGCTAACGGTGAAGCGCAAAAATACGATGGTTGCTGCCGTGATTTGCCTGCCCGTGACACTG
ATGAGCCTTTTGTGGTGCGTTTTAAAAATCCAATCGGTGGTTCAGTGGTATTTGATGACCATGTTCGTGGTCGTATTGAA
TTTTCTAATGACGCATTAGATGATTTGATCATTGCTCGTACCGACGGCGTGCCAACATATAACTTCTGCGTAGTCGTTGA
TGATTGGGATATGGGGATTACCTGTGTGGTGCGTGGTGAAGATCATATCAATAATACGCCTCGTCAAATTAACATCCTCA
AAGCGTTAGGTGCACCAATTCCTGAATATGCGCATGTATCGATGATTTTGGGTGATGACGGCGCTAAGTTATCTAAGCGC
CATGGCGCTGTTAGCGTAATGCAGTACCGTGATGATGGCTATTTACCTGAAGCGTTGCTCAACTATTTAGTGCGTTTAGG
TTGGTCACACGGTGATCAAGAAGTTTTCTCTTTAGAGGAAATGAAGCAATACTTTAAGCTAGACGATATTAATAAGGCGC
CTTCAGCCTTTAATACCGATAAGCTGGTTTGGTTGAACCAACACTATATTAAGACGCTTGACCCTGAATATGTGGCAACT
CACTTACAATGGCACATGGACGACCAAAAGATTGATACCTCAAATGGTCCAGCATTAGCCGAAGTGGTCACAGCATTAGC
AGAACGCGCTAAGACGTTAAAGGAATTAGCCGCTTCTAGTCGTTACTTCTATGAAGACTTTGCAGACTTTGATGCTGAGC
AGGCGAAAAAACATTTACGTGGTGTTGCGTTAGAACCGTTGCAATTGGTACAACAAAAATTAGCCGCATTAACTGAATGG
ACGGTTGAAGCAATACATCAGGCAATTGAACAAACGGCAACTGAATTAGATGTAGGTATGGGGAAAGTTGGCATGCCTCT
GCGTGTTTCTGTAACGGGAGCAGGGCAGTCTCCAGGTTTAGATATCACATTATTTTTAATAGGAAGAAGTCGCTCTGAGC
AAAGAATATCCAAAGCGATTGAATTTGTAGCAGATAGAATAAATTCCTAA

Upstream 100 bases:

>100_bases
TTGCCCCATTTGCAGGGCCTGCTTTGACTGTTAGAATAAGCCCACTTTTTATCGTACTTGTCTTTATTTAGACGACTTTT
ATATAGGTTAGTGTCCCATT

Downstream 100 bases:

>100_bases
AAAACGAGTTGACACTTTTAGGTGTGCTGCATAGAATGCGCCACGCAGTTGAGACACAGGCTGGCTAAAGCTTGCAGTGC
ACTTAATTGAGTTAAATAAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTTKTRFAPSPTGFLHVGGARTALYSWLQARANNGEFVLRIEDTDIERSTQAACDAILEGMNWLGLTWDEGPYYQTKRFD
RYNEIIAQMLEKGTAYKCYCSRERIDALRESQAANGEAQKYDGCCRDLPARDTDEPFVVRFKNPIGGSVVFDDHVRGRIE
FSNDALDDLIIARTDGVPTYNFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEVFSLEEMKQYFKLDDINKAPSAFNTDKLVWLNQHYIKTLDPEYVAT
HLQWHMDDQKIDTSNGPALAEVVTALAERAKTLKELAASSRYFYEDFADFDAEQAKKHLRGVALEPLQLVQQKLAALTEW
TVEAIHQAIEQTATELDVGMGKVGMPLRVSVTGAGQSPGLDITLFLIGRSRSEQRISKAIEFVADRINS

Sequences:

>Translated_469_residues
MTTKTRFAPSPTGFLHVGGARTALYSWLQARANNGEFVLRIEDTDIERSTQAACDAILEGMNWLGLTWDEGPYYQTKRFD
RYNEIIAQMLEKGTAYKCYCSRERIDALRESQAANGEAQKYDGCCRDLPARDTDEPFVVRFKNPIGGSVVFDDHVRGRIE
FSNDALDDLIIARTDGVPTYNFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEVFSLEEMKQYFKLDDINKAPSAFNTDKLVWLNQHYIKTLDPEYVAT
HLQWHMDDQKIDTSNGPALAEVVTALAERAKTLKELAASSRYFYEDFADFDAEQAKKHLRGVALEPLQLVQQKLAALTEW
TVEAIHQAIEQTATELDVGMGKVGMPLRVSVTGAGQSPGLDITLFLIGRSRSEQRISKAIEFVADRINS
>Mature_468_residues
TTKTRFAPSPTGFLHVGGARTALYSWLQARANNGEFVLRIEDTDIERSTQAACDAILEGMNWLGLTWDEGPYYQTKRFDR
YNEIIAQMLEKGTAYKCYCSRERIDALRESQAANGEAQKYDGCCRDLPARDTDEPFVVRFKNPIGGSVVFDDHVRGRIEF
SNDALDDLIIARTDGVPTYNFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKRH
GAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEVFSLEEMKQYFKLDDINKAPSAFNTDKLVWLNQHYIKTLDPEYVATH
LQWHMDDQKIDTSNGPALAEVVTALAERAKTLKELAASSRYFYEDFADFDAEQAKKHLRGVALEPLQLVQQKLAALTEWT
VEAIHQAIEQTATELDVGMGKVGMPLRVSVTGAGQSPGLDITLFLIGRSRSEQRISKAIEFVADRINS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=332, Percent_Identity=33.7349397590361, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI4826960, Length=100, Percent_Identity=35, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788743, Length=469, Percent_Identity=73.134328358209, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=39.6694214876033, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1786895, Length=218, Percent_Identity=24.7706422018349, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=475, Percent_Identity=30.7368421052632, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6324540, Length=494, Percent_Identity=30.7692307692308, Blast_Score=249, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6321192, Length=229, Percent_Identity=27.9475982532751, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=118, Percent_Identity=29.6610169491525, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24665321, Length=488, Percent_Identity=29.9180327868852, Blast_Score=240, Evalue=1e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 52672; Mature: 52541

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKTRFAPSPTGFLHVGGARTALYSWLQARANNGEFVLRIEDTDIERSTQAACDAILEG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHC
MNWLGLTWDEGPYYQTKRFDRYNEIIAQMLEKGTAYKCYCSRERIDALRESQAANGEAQK
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCCHH
YDGCCRDLPARDTDEPFVVRFKNPIGGSVVFDDHVRGRIEFSNDALDDLIIARTDGVPTY
HCCHHHCCCCCCCCCCEEEEECCCCCCCEEEECCCCCEEEECCCCHHHEEEEECCCCCCE
NFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
EEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEVFSLEEMKQYFKLDDINKAPSAFNTD
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
KLVWLNQHYIKTLDPEYVATHLQWHMDDQKIDTSNGPALAEVVTALAERAKTLKELAASS
EEEEECCHHHHHCCHHHHHHHHHEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RYFYEDFADFDAEQAKKHLRGVALEPLQLVQQKLAALTEWTVEAIHQAIEQTATELDVGM
CHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKVGMPLRVSVTGAGQSPGLDITLFLIGRSRSEQRISKAIEFVADRINS
CCCCCCEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTKTRFAPSPTGFLHVGGARTALYSWLQARANNGEFVLRIEDTDIERSTQAACDAILEG
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHC
MNWLGLTWDEGPYYQTKRFDRYNEIIAQMLEKGTAYKCYCSRERIDALRESQAANGEAQK
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCCHH
YDGCCRDLPARDTDEPFVVRFKNPIGGSVVFDDHVRGRIEFSNDALDDLIIARTDGVPTY
HCCHHHCCCCCCCCCCEEEEECCCCCCCEEEECCCCCEEEECCCCHHHEEEEECCCCCCE
NFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
EEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEVFSLEEMKQYFKLDDINKAPSAFNTD
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
KLVWLNQHYIKTLDPEYVATHLQWHMDDQKIDTSNGPALAEVVTALAERAKTLKELAASS
EEEEECCHHHHHCCHHHHHHHHHEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RYFYEDFADFDAEQAKKHLRGVALEPLQLVQQKLAALTEWTVEAIHQAIEQTATELDVGM
CHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKVGMPLRVSVTGAGQSPGLDITLFLIGRSRSEQRISKAIEFVADRINS
CCCCCCEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA