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The map label for this gene is hisS

Identifier: 120598179

GI number: 120598179

Start: 1535723

End: 1537000

Strand: Direct

Name: hisS

Synonym: Sputw3181_1356

Alternate gene names: 120598179

Gene position: 1535723-1537000 (Clockwise)

Preceding gene: 120598178

Following gene: 120598180

Centisome position: 32.62

GC content: 47.1

Gene sequence:

>1278_bases
GTGGCAAAACAGATCCAAGCGATTCGCGGAATGAATGACATTCTGCCAACCCAAAGTCCTCTATGGCAAAAAGTAGAAGC
GGTACTTCGTTCAAGCGTCAGTGCTTATGGTTACAGCGAAATCCGCACTCCAATAGTGGAAAATACCGATCTTTTTAAAC
GCTCTATCGGTGAAGTGACTGATATTGTTGAAAAAGAAATGTACACTTTCGAAGATCGTAATGGCGATAGCCTGACCTTA
CGTCCCGAAGGTACCGCCTCTACTGTGCGTGCGGGTAATGAGCATGGTTTGCTCTATAACCAAGAACAGCGCTTGTGGTA
TATGGGGCCAATGTTCCGCCATGAACGTCCACAAAAGGGGCGTTACCGTCAATTCCACCAATTTGGTGTTGAAGTTTATG
GTATTGGCAGCGCAGATATTGACGCCGAAGTGCTTATGTTATCGGCGCGTCTATGGGAAAAGCTGGGAATTAGCGAACAT
GTGACGCTTGAGCTTAATACCTTAGGTGATCCCGCTGAGCGTGCGGCTTACCGCGATGCCTTGATTGCTTTTTTAGAGCA
GCATAAAGATAAGTTAGATGAAGACAGTCAACGCCGTATGTACAGCAATCCACTGCGCGTACTGGATTCTAAAGATCCTC
AAGTTCAGGGGATTTTAACCGATGCGCCAGCGCTGATGGATTACTTAGGTGAAGAATCTTCACAACATTTTGCACAATTA
CGTGAACTCCTAGACGCCGTTGGCATCCAATACCGAGTTAATCCTCGCTTAGTCCGTGGTCTCGATTATTATAATCGCAC
TGTATTTGAATGGGTTACCAACAGCTTAGGCTCACAAGGCACGGTACTTGCGGGCGGACGCTATGATGGTTTAGTGGCGC
AATTAGGCGGTAAAGATACCCCAGCGGTCGGTTTTGCGATGGGGCTTGAGCGTATTGTATTGCTGCTTGAAACCTTGGGC
CTTACACAGGACATTCCCGCGGCAGTTGATGTGTATGTCACCGCCATGGGGGAGAATTGTCTTGTCGAAGCCATTAAAGT
TGCACAGGAATTACGTAGTGCGCTGCCACACCTTAAAGTGATGAGTCACTGTGGTGGCGGTAACGTGAAGAAACAAATGA
AGCGCGCCGACAAAAGCGGTGCGAGTGTGGCATTACTGATAGGTGAAGAAGAACTTGCCGAAGGTATGGTCACAGTGAAA
CATTTACGCAATGACAACGAACAACAACAGGTTGCTCGAAATGCCTTAAGTGCATTTTTAGCTGAACTTACCAAATAA

Upstream 100 bases:

>100_bases
GTGTGCCCCGAAACCGATGGGGCCATTTTACGTTTCGTCTTCAGGCGTCTATAATGCGGAACATTTTTATTAATCTAGCG
AAATCGGACGAGTCGATACA

Downstream 100 bases:

>100_bases
AGGGGATGTGCGCGTGGAAATTTATAGCACAGAAGAACAACAAGTTGATGCTATCAAACAGTTCTGGAAAGATTATGGAA
CTTCCATTTTAATAGGTGCT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MAKQIQAIRGMNDILPTQSPLWQKVEAVLRSSVSAYGYSEIRTPIVENTDLFKRSIGEVTDIVEKEMYTFEDRNGDSLTL
RPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQFGVEVYGIGSADIDAEVLMLSARLWEKLGISEH
VTLELNTLGDPAERAAYRDALIAFLEQHKDKLDEDSQRRMYSNPLRVLDSKDPQVQGILTDAPALMDYLGEESSQHFAQL
RELLDAVGIQYRVNPRLVRGLDYYNRTVFEWVTNSLGSQGTVLAGGRYDGLVAQLGGKDTPAVGFAMGLERIVLLLETLG
LTQDIPAAVDVYVTAMGENCLVEAIKVAQELRSALPHLKVMSHCGGGNVKKQMKRADKSGASVALLIGEEELAEGMVTVK
HLRNDNEQQQVARNALSAFLAELTK

Sequences:

>Translated_425_residues
MAKQIQAIRGMNDILPTQSPLWQKVEAVLRSSVSAYGYSEIRTPIVENTDLFKRSIGEVTDIVEKEMYTFEDRNGDSLTL
RPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQFGVEVYGIGSADIDAEVLMLSARLWEKLGISEH
VTLELNTLGDPAERAAYRDALIAFLEQHKDKLDEDSQRRMYSNPLRVLDSKDPQVQGILTDAPALMDYLGEESSQHFAQL
RELLDAVGIQYRVNPRLVRGLDYYNRTVFEWVTNSLGSQGTVLAGGRYDGLVAQLGGKDTPAVGFAMGLERIVLLLETLG
LTQDIPAAVDVYVTAMGENCLVEAIKVAQELRSALPHLKVMSHCGGGNVKKQMKRADKSGASVALLIGEEELAEGMVTVK
HLRNDNEQQQVARNALSAFLAELTK
>Mature_424_residues
AKQIQAIRGMNDILPTQSPLWQKVEAVLRSSVSAYGYSEIRTPIVENTDLFKRSIGEVTDIVEKEMYTFEDRNGDSLTLR
PEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQFGVEVYGIGSADIDAEVLMLSARLWEKLGISEHV
TLELNTLGDPAERAAYRDALIAFLEQHKDKLDEDSQRRMYSNPLRVLDSKDPQVQGILTDAPALMDYLGEESSQHFAQLR
ELLDAVGIQYRVNPRLVRGLDYYNRTVFEWVTNSLGSQGTVLAGGRYDGLVAQLGGKDTPAVGFAMGLERIVLLLETLGL
TQDIPAAVDVYVTAMGENCLVEAIKVAQELRSALPHLKVMSHCGGGNVKKQMKRADKSGASVALLIGEEELAEGMVTVKH
LRNDNEQQQVARNALSAFLAELTK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=448, Percent_Identity=24.5535714285714, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI15029520, Length=476, Percent_Identity=23.9495798319328, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788861, Length=423, Percent_Identity=67.3758865248227, Blast_Score=591, Evalue=1e-170,
Organism=Caenorhabditis elegans, GI71993693, Length=459, Percent_Identity=26.5795206971678, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71993686, Length=459, Percent_Identity=26.5795206971678, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325290, Length=455, Percent_Identity=24.8351648351648, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47398; Mature: 47266

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQIQAIRGMNDILPTQSPLWQKVEAVLRSSVSAYGYSEIRTPIVENTDLFKRSIGEVT
CCCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHH
DIVEKEMYTFEDRNGDSLTLRPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQFGVEVYGIGSADIDAEVLMLSARLWEKLGISEHVTLELNTLGDPAERAAYRDA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
LIAFLEQHKDKLDEDSQRRMYSNPLRVLDSKDPQVQGILTDAPALMDYLGEESSQHFAQL
HHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHH
RELLDAVGIQYRVNPRLVRGLDYYNRTVFEWVTNSLGSQGTVLAGGRYDGLVAQLGGKDT
HHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHCCCCCC
PAVGFAMGLERIVLLLETLGLTQDIPAAVDVYVTAMGENCLVEAIKVAQELRSALPHLKV
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHH
MSHCGGGNVKKQMKRADKSGASVALLIGEEELAEGMVTVKHLRNDNEQQQVARNALSAFL
HHHCCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AELTK
HHHCC
>Mature Secondary Structure 
AKQIQAIRGMNDILPTQSPLWQKVEAVLRSSVSAYGYSEIRTPIVENTDLFKRSIGEVT
CCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHH
DIVEKEMYTFEDRNGDSLTLRPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQFGVEVYGIGSADIDAEVLMLSARLWEKLGISEHVTLELNTLGDPAERAAYRDA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
LIAFLEQHKDKLDEDSQRRMYSNPLRVLDSKDPQVQGILTDAPALMDYLGEESSQHFAQL
HHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHH
RELLDAVGIQYRVNPRLVRGLDYYNRTVFEWVTNSLGSQGTVLAGGRYDGLVAQLGGKDT
HHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHCCCCCC
PAVGFAMGLERIVLLLETLGLTQDIPAAVDVYVTAMGENCLVEAIKVAQELRSALPHLKV
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHH
MSHCGGGNVKKQMKRADKSGASVALLIGEEELAEGMVTVKHLRNDNEQQQVARNALSAFL
HHHCCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AELTK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]