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The map label for this gene is ispG

Identifier: 120598178

GI number: 120598178

Start: 1534477

End: 1535592

Strand: Direct

Name: ispG

Synonym: Sputw3181_1355

Alternate gene names: 120598178

Gene position: 1534477-1535592 (Clockwise)

Preceding gene: 120598177

Following gene: 120598179

Centisome position: 32.59

GC content: 46.06

Gene sequence:

>1116_bases
ATGTATAATGAAACTCCCATAAAAAGACGTCCTTCCACCCGCATTTATGTGGGTAATGTACCGATTGGTGATGGGGCGCC
GATTGCCGTTCAGTCAATGACCAATACTAAGACTACCGATGTTGAGGCTACTATTGCTCAAATTCGCGCCTTAGAAAAAG
TCGGTGCCGACATAGTGCGCGTTTCCGTACCGACTATGGATGCCGCTGAAGCCTTTAAAATCATAAAACAATCTGTAAAT
GTTCCCTTAGTGGCAGATATTCACTTTGATTATCGTATTGCCCTCAAAGTTGCTGAATACGGTGTCGATTGTTTACGCAT
CAACCCAGGTAACATAGGCAATGAAGAACGTATTCGCAGTGTGGTTGAGTGCGCCCGGGATAAAAATATCCCAATCCGTA
TTGGCGTCAATGGCGGTTCATTAGAAAAAGATTTGATGGACAAATACAAGGAGCCGACCCCCGAGGCATTGCTCGAATCG
GCAATGCGCCATGTGGATATTTTAGATCGCATGAACTTCGACCAATTTAAGGTCAGTGTTAAGGCGTCGGATGTGTTCTT
GGCGGTTGAGTCCTATCGTTTATTGGCAAAACAAATCCGCCAACCGCTGCATTTAGGCATTACCGAAGCGGGTGGCGCGC
GCGCAGGCTCAGTAAAATCAGCCGTTGGCTTAGGCATGTTATTGGCCGAAGGTATTGGGGATACATTACGTATTTCCCTC
GCTGCCGATCCCGTTGAAGAAATCAAAGTCGGTTTTGATATCTTAAAGTCGCTACGTATTCGTAGTCGCGGCATTAATTT
TATTGCCTGTCCATCTTGCTCGCGCCAAGAGTTTGATGTGATCAGCACGGTGAATGAATTAGAGCGCCGCTTAGAAGATG
TCACTACAGCGATGGATGTATCTATTATTGGCTGCGTAGTGAATGGCCCCGGTGAGGCTTTAGTGTCGCATATTGGTTTA
ACCGGTGGACACAATAAGAGTGGCTACTACGATGAGGGCGAACGTCAAAAAGAACGTTTTGATAATGACAATTTAGTCGA
TTCCCTCGAAGCCAAAATTCGTGCTAAGGCGAGCCAACTGGCAAACCGTATCCAAGTAAAAGATACAACCGAGTAA

Upstream 100 bases:

>100_bases
GCTGATTTTCCTAAAGGCCGAGTTGCTCGTTTAACGCTTCCTAAAGCGTAAGATTGACTAACAGGCGCAGCAGAACGTAC
CGAAATAGAGCGTATTAAGT

Downstream 100 bases:

>100_bases
AATGTATGCATTGATGCCAGAGACTTTGAGGTGTGCCCCGAAACCGATGGGGCCATTTTACGTTTCGTCTTCAGGCGTCT
ATAATGCGGAACATTTTTAT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MYNETPIKRRPSTRIYVGNVPIGDGAPIAVQSMTNTKTTDVEATIAQIRALEKVGADIVRVSVPTMDAAEAFKIIKQSVN
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRSVVECARDKNIPIRIGVNGGSLEKDLMDKYKEPTPEALLES
AMRHVDILDRMNFDQFKVSVKASDVFLAVESYRLLAKQIRQPLHLGITEAGGARAGSVKSAVGLGMLLAEGIGDTLRISL
AADPVEEIKVGFDILKSLRIRSRGINFIACPSCSRQEFDVISTVNELERRLEDVTTAMDVSIIGCVVNGPGEALVSHIGL
TGGHNKSGYYDEGERQKERFDNDNLVDSLEAKIRAKASQLANRIQVKDTTE

Sequences:

>Translated_371_residues
MYNETPIKRRPSTRIYVGNVPIGDGAPIAVQSMTNTKTTDVEATIAQIRALEKVGADIVRVSVPTMDAAEAFKIIKQSVN
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRSVVECARDKNIPIRIGVNGGSLEKDLMDKYKEPTPEALLES
AMRHVDILDRMNFDQFKVSVKASDVFLAVESYRLLAKQIRQPLHLGITEAGGARAGSVKSAVGLGMLLAEGIGDTLRISL
AADPVEEIKVGFDILKSLRIRSRGINFIACPSCSRQEFDVISTVNELERRLEDVTTAMDVSIIGCVVNGPGEALVSHIGL
TGGHNKSGYYDEGERQKERFDNDNLVDSLEAKIRAKASQLANRIQVKDTTE
>Mature_371_residues
MYNETPIKRRPSTRIYVGNVPIGDGAPIAVQSMTNTKTTDVEATIAQIRALEKVGADIVRVSVPTMDAAEAFKIIKQSVN
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRSVVECARDKNIPIRIGVNGGSLEKDLMDKYKEPTPEALLES
AMRHVDILDRMNFDQFKVSVKASDVFLAVESYRLLAKQIRQPLHLGITEAGGARAGSVKSAVGLGMLLAEGIGDTLRISL
AADPVEEIKVGFDILKSLRIRSRGINFIACPSCSRQEFDVISTVNELERRLEDVTTAMDVSIIGCVVNGPGEALVSHIGL
TGGHNKSGYYDEGERQKERFDNDNLVDSLEAKIRAKASQLANRIQVKDTTE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=369, Percent_Identity=84.2818428184282, Blast_Score=637, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 40619; Mature: 40619

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNETPIKRRPSTRIYVGNVPIGDGAPIAVQSMTNTKTTDVEATIAQIRALEKVGADIVR
CCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEE
VSVPTMDAAEAFKIIKQSVNVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRS
EECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHHH
VVECARDKNIPIRIGVNGGSLEKDLMDKYKEPTPEALLESAMRHVDILDRMNFDQFKVSV
HHHHHHCCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFLAVESYRLLAKQIRQPLHLGITEAGGARAGSVKSAVGLGMLLAEGIGDTLRISL
EECEEEEEHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
AADPVEEIKVGFDILKSLRIRSRGINFIACPSCSRQEFDVISTVNELERRLEDVTTAMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCE
SIIGCVVNGPGEALVSHIGLTGGHNKSGYYDEGERQKERFDNDNLVDSLEAKIRAKASQL
EEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
ANRIQVKDTTE
HHHCCCCCCCC
>Mature Secondary Structure
MYNETPIKRRPSTRIYVGNVPIGDGAPIAVQSMTNTKTTDVEATIAQIRALEKVGADIVR
CCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEE
VSVPTMDAAEAFKIIKQSVNVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRS
EECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHHH
VVECARDKNIPIRIGVNGGSLEKDLMDKYKEPTPEALLESAMRHVDILDRMNFDQFKVSV
HHHHHHCCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFLAVESYRLLAKQIRQPLHLGITEAGGARAGSVKSAVGLGMLLAEGIGDTLRISL
EECEEEEEHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
AADPVEEIKVGFDILKSLRIRSRGINFIACPSCSRQEFDVISTVNELERRLEDVTTAMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCE
SIIGCVVNGPGEALVSHIGLTGGHNKSGYYDEGERQKERFDNDNLVDSLEAKIRAKASQL
EEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
ANRIQVKDTTE
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA