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The map label for this gene is ymfK [C]

Identifier: 120598006

GI number: 120598006

Start: 1333192

End: 1333839

Strand: Direct

Name: ymfK [C]

Synonym: Sputw3181_1183

Alternate gene names: 120598006

Gene position: 1333192-1333839 (Clockwise)

Preceding gene: 120597998

Following gene: 120598007

Centisome position: 28.32

GC content: 48.15

Gene sequence:

>648_bases
ATGAAAACTTTATCCGAACGACTAAACCATGCCTTGCAGCTTACTGGGGTGACTCAGTCTGAGTTGGCTCGTCGCATTGG
TATCAAACAGCAGTCGATCAGCCAGATTTGCTCTGGTAAATCGGCTAGGTCTCGTTACACCATGCAGATCGCGGAGGCGC
TTCGCGTGAATGCTCATTGGCTCGCCACAGGTGATGGCGAGATTGGCTTGGGGGTCGGTAATGTAGAAGTCGGGCCTGAT
ATTAAGGGAAGAATTCCTCTCATTAACTGGGTTCAGGCCGGTGATTGGACTGAAATAGCGGAGGGATTTGCCCATGAAGA
TGCTGAGGAGTGGCGTGAAGTCACTGGGAAAGCACATGAGGGTTGTTTCGCACTTCGCGTAAAAGGCGACAGTATGGAAA
ATCCAAGCGGAAAAAAATCCATACCTGAGGGAGCAGTGATCGTTGTTGATCCTGAGTTACCGTACTCTTCAGGTTCATTG
GTTGTTGCGCGTTTGGATGATTCGAAAGAAGCAACCTTTAAGCAGTTGGTTATTGATGGTGAACAGAAGTACCTAAAACC
TTTGAACCCACAATACCCTGCAATACCGATCAACGGCAACTGCACCATCATTGGTGTAGTACGACAAGCTATCATCGATT
TTTGGTAG

Upstream 100 bases:

>100_bases
TAAAAACTGTTACAAGATTGAATGTTACAGTTTAAACTGTAGATAAGTCAACAGTTAAAATTGTTGAAAGGCTACAGTTT
TATTTGTAGAATACGGGCTT

Downstream 100 bases:

>100_bases
CGAAGGAATTTGTGGTTTAGCCACAGTTGTTCATGAGCTGAAGAAGCAACGATTGCAAACAGCTACAACTGAGCATTGGC
GCACGCTGAGAGTAAATGGT

Product: putative prophage repressor

Products: NA

Alternate protein names: ORF-96 protein

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKTLSERLNHALQLTGVTQSELARRIGIKQQSISQICSGKSARSRYTMQIAEALRVNAHWLATGDGEIGLGVGNVEVGPD
IKGRIPLINWVQAGDWTEIAEGFAHEDAEEWREVTGKAHEGCFALRVKGDSMENPSGKKSIPEGAVIVVDPELPYSSGSL
VVARLDDSKEATFKQLVIDGEQKYLKPLNPQYPAIPINGNCTIIGVVRQAIIDFW

Sequences:

>Translated_215_residues
MKTLSERLNHALQLTGVTQSELARRIGIKQQSISQICSGKSARSRYTMQIAEALRVNAHWLATGDGEIGLGVGNVEVGPD
IKGRIPLINWVQAGDWTEIAEGFAHEDAEEWREVTGKAHEGCFALRVKGDSMENPSGKKSIPEGAVIVVDPELPYSSGSL
VVARLDDSKEATFKQLVIDGEQKYLKPLNPQYPAIPINGNCTIIGVVRQAIIDFW
>Mature_215_residues
MKTLSERLNHALQLTGVTQSELARRIGIKQQSISQICSGKSARSRYTMQIAEALRVNAHWLATGDGEIGLGVGNVEVGPD
IKGRIPLINWVQAGDWTEIAEGFAHEDAEEWREVTGKAHEGCFALRVKGDSMENPSGKKSIPEGAVIVVDPELPYSSGSL
VVARLDDSKEATFKQLVIDGEQKYLKPLNPQYPAIPINGNCTIIGVVRQAIIDFW

Specific function: May control the expression of the integrase and inhibit excision of the mobile element R391, and regulate the expression of other genes as well

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787391, Length=125, Percent_Identity=43.2, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TR96_PRORE (Q79RI9)

Other databases:

- EMBL:   U13633
- EMBL:   AY090559
- ProteinModelPortal:   Q79RI9
- SMR:   Q79RI9
- MEROPS:   S24.002
- GO:   GO:0006350
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- Gene3D:   G3DSA:2.10.109.10
- SMART:   SM00530

Pfam domain/function: PF01381 HTH_3; PF00717 Peptidase_S24; SSF47413 Lambda_like_DNA; SSF51306 Pept_S24_S26_C

EC number: NA

Molecular weight: Translated: 23491; Mature: 23491

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLSERLNHALQLTGVTQSELARRIGIKQQSISQICSGKSARSRYTMQIAEALRVNAHW
CCCHHHHHHHEEEECCCCHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEE
LATGDGEIGLGVGNVEVGPDIKGRIPLINWVQAGDWTEIAEGFAHEDAEEWREVTGKAHE
EEECCCCEEEEECCEEECCCCCCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHCCCCCC
GCFALRVKGDSMENPSGKKSIPEGAVIVVDPELPYSSGSLVVARLDDSKEATFKQLVIDG
CEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHCCC
EQKYLKPLNPQYPAIPINGNCTIIGVVRQAIIDFW
CHHHHCCCCCCCCEEEECCCEEEEHHHHHHHHHCC
>Mature Secondary Structure
MKTLSERLNHALQLTGVTQSELARRIGIKQQSISQICSGKSARSRYTMQIAEALRVNAHW
CCCHHHHHHHEEEECCCCHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEE
LATGDGEIGLGVGNVEVGPDIKGRIPLINWVQAGDWTEIAEGFAHEDAEEWREVTGKAHE
EEECCCCEEEEECCEEECCCCCCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHCCCCCC
GCFALRVKGDSMENPSGKKSIPEGAVIVVDPELPYSSGSLVVARLDDSKEATFKQLVIDG
CEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHCCC
EQKYLKPLNPQYPAIPINGNCTIIGVVRQAIIDFW
CHHHHCCCCCCCCEEEECCCEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11157923; 12193633