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The map label for this gene is acrA [H]

Identifier: 120597956

GI number: 120597956

Start: 1278712

End: 1280022

Strand: Direct

Name: acrA [H]

Synonym: Sputw3181_1129

Alternate gene names: 120597956

Gene position: 1278712-1280022 (Clockwise)

Preceding gene: 120597953

Following gene: 120597957

Centisome position: 27.16

GC content: 62.4

Gene sequence:

>1311_bases
ATGCATAAAAAAATCAGGATCATTTTGGTAGCTAGCGTCATCGCGGCCGTCGGGGGGATAGCGGTGGCTGCCCGAGATGG
CAAGGATGAAAGTAAACCAGCAGCACCACCAGCTGGCGCACCGATGGCACCGCAGGTGCCTGTGGCTGAGGCCATCACGC
GCACAATCACGCCGTCAACTGAGTTCACCGGCTTTTTGGCCGCACCGAAGGTGGTCGAGTTGCGCTCGCGTGTGGGGGGG
GCCGTCGACGCGGTGAGCGTGCCCGAGGGAAGTCTGGTACGCAAGGGGCAATTGTTGTTCCAGATTGACCCACGGCCTTT
CCAAGTCGCGCTCGACACCGCCACGGCGCAGTTGCGGCAGGCTGAGGTGCTGTCCAGTCAGGCTCAGGCCGACTTTGATC
GTGCCGAGCGGCTTGTTGCCACTGGCGCCCTGTCTCGGAAGACCTATGACGATGCAGTCTCCGCACGCAATGCGCGTCAG
GCGCAGGTACAGGCGGCAAAGGCCACAGTCGCAGCGGCGCAGCTCGACCTGTCCTACGCGCGGGTATCGGCACCAATTGC
CGGGCGCGTGGACCGAGTGCTGGTGACCGAGGGCAACCTGGTCAGCGGCGGCGGTGCAGGAGCAGCCACGCTACTTACCA
CGATCGTGTCCATCGATCCTGTGCACGTCTATTTCGACATCGATGAGGCAACCTACGTCAATGTGGTGAGCCGCTCGAGA
CCTGGTTCAGGGACTCATGCTTCGTTGCCCGTTCAAGTAGGCTTGAGCACTGACAGAAACTTTCCGTACAAGGGCGTGCT
CGACTTCGTCGGCAATGCGATTGATAGAAGTACCGGCACGATCCGCGCTCGCGCGGTCGTCCCCAACCCTGATGGACGGC
TGACGCCTGGGCTGTTTGCGAGGGCCAAGCTCTCCACCGCAAGCTCTCGGCAGGCCGTCCTCATCGACGACCAAGCGGTT
GGCACCGATCAAGGCCGAAACTATGTTCTGGTCGTGGGTGAAAACAACCAAGCTCAGTATCGTCCGATTGAGCTGGGTCC
CGTAGTCGACGGTCTGCGCGTTATAAACGGTGGCCTGCAGGGTGGCGAAAAAATCATAATCAAAGGCCTCGTACGACCTG
GTATGACTGTCACGCCGCGCATGGTGCCCATGCAGGGGCCGGCAGCACCGGCCGCCGGCGCTCCCGACGCGACTAAGACT
GCGGCTAGTGCCCCGGCAGTCCCTGCGGAGTCTGCCAAGGCCGCTGGGGCGGCTGGGGCCGATCAGGTCAAGGCTGACAG
CGCAGCTGGCTCAGCGGAGGCTCGCAAATGA

Upstream 100 bases:

>100_bases
AAAAGGCTTGACCTTGACGTGGTTGTAAGGGCTAGCCTTTAGAAACATATGCGAACCGCTTCGGATGGACCGAAGCCCCG
TAATAAAAAAGGTAACTTCC

Downstream 100 bases:

>100_bases
ATTTCCCCCGTTTCTTCATCAACCGCCCTATCTTCGCAATCGTCCTTTCGGTGCTGATGCTGATCGCGGGCGCGATCAGC
TACTTCCAGTTGCCACTGAG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MHKKIRIILVASVIAAVGGIAVAARDGKDESKPAAPPAGAPMAPQVPVAEAITRTITPSTEFTGFLAAPKVVELRSRVGG
AVDAVSVPEGSLVRKGQLLFQIDPRPFQVALDTATAQLRQAEVLSSQAQADFDRAERLVATGALSRKTYDDAVSARNARQ
AQVQAAKATVAAAQLDLSYARVSAPIAGRVDRVLVTEGNLVSGGGAGAATLLTTIVSIDPVHVYFDIDEATYVNVVSRSR
PGSGTHASLPVQVGLSTDRNFPYKGVLDFVGNAIDRSTGTIRARAVVPNPDGRLTPGLFARAKLSTASSRQAVLIDDQAV
GTDQGRNYVLVVGENNQAQYRPIELGPVVDGLRVINGGLQGGEKIIIKGLVRPGMTVTPRMVPMQGPAAPAAGAPDATKT
AASAPAVPAESAKAAGAAGADQVKADSAAGSAEARK

Sequences:

>Translated_436_residues
MHKKIRIILVASVIAAVGGIAVAARDGKDESKPAAPPAGAPMAPQVPVAEAITRTITPSTEFTGFLAAPKVVELRSRVGG
AVDAVSVPEGSLVRKGQLLFQIDPRPFQVALDTATAQLRQAEVLSSQAQADFDRAERLVATGALSRKTYDDAVSARNARQ
AQVQAAKATVAAAQLDLSYARVSAPIAGRVDRVLVTEGNLVSGGGAGAATLLTTIVSIDPVHVYFDIDEATYVNVVSRSR
PGSGTHASLPVQVGLSTDRNFPYKGVLDFVGNAIDRSTGTIRARAVVPNPDGRLTPGLFARAKLSTASSRQAVLIDDQAV
GTDQGRNYVLVVGENNQAQYRPIELGPVVDGLRVINGGLQGGEKIIIKGLVRPGMTVTPRMVPMQGPAAPAAGAPDATKT
AASAPAVPAESAKAAGAAGADQVKADSAAGSAEARK
>Mature_436_residues
MHKKIRIILVASVIAAVGGIAVAARDGKDESKPAAPPAGAPMAPQVPVAEAITRTITPSTEFTGFLAAPKVVELRSRVGG
AVDAVSVPEGSLVRKGQLLFQIDPRPFQVALDTATAQLRQAEVLSSQAQADFDRAERLVATGALSRKTYDDAVSARNARQ
AQVQAAKATVAAAQLDLSYARVSAPIAGRVDRVLVTEGNLVSGGGAGAATLLTTIVSIDPVHVYFDIDEATYVNVVSRSR
PGSGTHASLPVQVGLSTDRNFPYKGVLDFVGNAIDRSTGTIRARAVVPNPDGRLTPGLFARAKLSTASSRQAVLIDDQAV
GTDQGRNYVLVVGENNQAQYRPIELGPVVDGLRVINGGLQGGEKIIIKGLVRPGMTVTPRMVPMQGPAAPAAGAPDATKT
AASAPAVPAESAKAAGAAGADQVKADSAAGSAEARK

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=406, Percent_Identity=32.0197044334975, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1789665, Length=382, Percent_Identity=32.9842931937173, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789929, Length=343, Percent_Identity=32.3615160349854, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI87082036, Length=384, Percent_Identity=28.90625, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87081796, Length=329, Percent_Identity=27.0516717325228, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789637, Length=132, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44773; Mature: 44773

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKKIRIILVASVIAAVGGIAVAARDGKDESKPAAPPAGAPMAPQVPVAEAITRTITPST
CCCEEEEEHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
EFTGFLAAPKVVELRSRVGGAVDAVSVPEGSLVRKGQLLFQIDPRPFQVALDTATAQLRQ
CCCEEECCCHHHHHHHHCCCCEEEEECCCCCEEECCCEEEEECCCCEEEEEEHHHHHHHH
AEVLSSQAQADFDRAERLVATGALSRKTYDDAVSARNARQAQVQAAKATVAAAQLDLSYA
HHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RVSAPIAGRVDRVLVTEGNLVSGGGAGAATLLTTIVSIDPVHVYFDIDEATYVNVVSRSR
HHCCCCCCCCCEEEEECCCEEECCCCHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCC
PGSGTHASLPVQVGLSTDRNFPYKGVLDFVGNAIDRSTGTIRARAVVPNPDGRLTPGLFA
CCCCCCEEEEEEEECCCCCCCCHHHHHHHHCHHHCCCCCCEEEEEEECCCCCCCCCCHHH
RAKLSTASSRQAVLIDDQAVGTDQGRNYVLVVGENNQAQYRPIELGPVVDGLRVINGGLQ
EEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHCCCC
GGEKIIIKGLVRPGMTVTPRMVPMQGPAAPAAGAPDATKTAASAPAVPAESAKAAGAAGA
CCCEEEEEECCCCCCEECCEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCC
DQVKADSAAGSAEARK
CHHCCCCCCCCCCCCC
>Mature Secondary Structure
MHKKIRIILVASVIAAVGGIAVAARDGKDESKPAAPPAGAPMAPQVPVAEAITRTITPST
CCCEEEEEHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
EFTGFLAAPKVVELRSRVGGAVDAVSVPEGSLVRKGQLLFQIDPRPFQVALDTATAQLRQ
CCCEEECCCHHHHHHHHCCCCEEEEECCCCCEEECCCEEEEECCCCEEEEEEHHHHHHHH
AEVLSSQAQADFDRAERLVATGALSRKTYDDAVSARNARQAQVQAAKATVAAAQLDLSYA
HHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RVSAPIAGRVDRVLVTEGNLVSGGGAGAATLLTTIVSIDPVHVYFDIDEATYVNVVSRSR
HHCCCCCCCCCEEEEECCCEEECCCCHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCC
PGSGTHASLPVQVGLSTDRNFPYKGVLDFVGNAIDRSTGTIRARAVVPNPDGRLTPGLFA
CCCCCCEEEEEEEECCCCCCCCHHHHHHHHCHHHCCCCCCEEEEEEECCCCCCCCCCHHH
RAKLSTASSRQAVLIDDQAVGTDQGRNYVLVVGENNQAQYRPIELGPVVDGLRVINGGLQ
EEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHCCCC
GGEKIIIKGLVRPGMTVTPRMVPMQGPAAPAAGAPDATKTAASAPAVPAESAKAAGAAGA
CCCEEEEEECCCCCCEECCEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCC
DQVKADSAAGSAEARK
CHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]