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The map label for this gene is cobQ [H]

Identifier: 120597819

GI number: 120597819

Start: 1127793

End: 1129382

Strand: Direct

Name: cobQ [H]

Synonym: Sputw3181_0989

Alternate gene names: 120597819

Gene position: 1127793-1129382 (Clockwise)

Preceding gene: 120597818

Following gene: 120597820

Centisome position: 23.95

GC content: 49.43

Gene sequence:

>1590_bases
ATGCCTTCTGTAAATACAATGTCTTCTTTCACTATAGACAATACCGCCAATATTAATTCACCTCGGCGCGCGGCAGTCTT
GATGGTGCAGGGCACAACATCGGATGCTGGCAAGAGCACCTTAGTCGCAGGCATTTGTCGTTTGCTCGCTCGTCAGGGGA
TTAAGGTTGCGCCCTTTAAGCCGCAAAATATGGCACTCAATAGCGCTGTTACTATTGATGGCGGCGAGATTGGCCGCGCA
CAGGCCTTACAAGCCGTGGCCTGTTATCTTGAGCCCCATACCGATTTTAATCCCATACTGCTTAAACCAAGCTCGGACAC
GGGCGCGCAAATTATCGTTCATGGCAAAGCCCTGACGACACTCGAAGCCTCGGCCTTTTTTGGGCCTAAATCTAAGGATT
ACAAGGCTATGGCGCTTGGTGCTGTGCTGGAGTCCTTCGAGCGATTGACGGCCCAATATCAAACCATAGTGGTTGAAGGC
GCGGGCAGTCCGGCGGAGATCAATCTGCGTGAAGGTGATATTGCCAACATGGGATTTGCCGAAGTTGTGGATTGTCCTGT
GATTATCATTGCCGATATCGACAAGGGCGGCGTATTTGCCCATTTGGTCGGGACCTTGGCCTTGTTATCAGAATCTGAAC
AAGCACGGGTAAAGGGCTTTGTGATCAACCGTTTCCGTGGCGATATTAGCCTGTTGCAATCTGGCCTCGATTGGCTTGAA
ACTTATACAAAAAAGCCTGTTTTAGGTGTGTTGCCTTATCTGCATGATTTACATCTCGATGCCGAAGATGCGCTGATAAG
CTCGCCGACCAAAGGCGCTAACAGCCGCTTAAAAGTGCGGGTATTAGTGTACCCAAGGATCAGTAATCACACGGATTTTG
ATCCGCTGCGGCTGCATCCCGATATCGATTTTGACTATGTGTCATTACAAACCCAAGCCGCGAGTCACTCCACTCATCTG
CCCGCCGCTGATGTATTGATCTTGCCCGGCAGTAAGAATGTGCGCGCGGATTTGGCCTTTTTACGCCAGCAAGGCTGGGA
TAAAGATATCGCCACACACCTGCGTTACGGCGGAAAAGTGCTAGGCGTTTGTGGCGGCTATCAAATGCTAGGCCAAAGCA
TTGCCGATCCCTTAGGCATTGAAGACAGTGCGGGTGATACCGTTGGACTTGGCTACCTACCGCTGAGCACAGAACTCAAA
GCCGAAAAACAGTTACGGCGAGTGAGCGGTGAGCTAACGTTACTTGGGCAAAAGGTTGCTGTTAAGGGCTATGAAATCCA
CTGCGGTGAATCAAGCTATCTTATCCCTAGGCTTGAACAAACGAATGTACCCTTGCAGTTAATCCCTGATTCCACATCTA
TGAGCTTTGCCGATGGCCTGCAAAGTGATGACGGGCAAATCCTTGGCACTTATTTACATGGGCTGTTTGATAGCCCGGAT
GCGTGTCAGTTGATTTTGCGCTGGGCGGGATTAAACGATGCTAAGGCGATAGATATCAATAAAATACGTGAGCAGCAACT
CGATCGTTTAGCCGATGTGTTAGCCGAGCATTTAGACATGCAAAAATTACAGCCAATTTTAGAAGCATAA

Upstream 100 bases:

>100_bases
AACCACTTTAGCTTTAATCGATGCGTTTATAACGGCTTTGCATGAGTATTGGGGCTTAGCTTGGATGTTAATACCGCAAA
CTTAAGTAAGTCATTAATCT

Downstream 100 bases:

>100_bases
GTTTTAGAAGCATAAGTTTTAAAAGCAGACATTTTTAGCAGCAGAGTGAAGAGAAGACCATGACAGCACAGAACGACAAC
GAACAAGCGCAAATCAAGGC

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MPSVNTMSSFTIDNTANINSPRRAAVLMVQGTTSDAGKSTLVAGICRLLARQGIKVAPFKPQNMALNSAVTIDGGEIGRA
QALQAVACYLEPHTDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGPKSKDYKAMALGAVLESFERLTAQYQTIVVEG
AGSPAEINLREGDIANMGFAEVVDCPVIIIADIDKGGVFAHLVGTLALLSESEQARVKGFVINRFRGDISLLQSGLDWLE
TYTKKPVLGVLPYLHDLHLDAEDALISSPTKGANSRLKVRVLVYPRISNHTDFDPLRLHPDIDFDYVSLQTQAASHSTHL
PAADVLILPGSKNVRADLAFLRQQGWDKDIATHLRYGGKVLGVCGGYQMLGQSIADPLGIEDSAGDTVGLGYLPLSTELK
AEKQLRRVSGELTLLGQKVAVKGYEIHCGESSYLIPRLEQTNVPLQLIPDSTSMSFADGLQSDDGQILGTYLHGLFDSPD
ACQLILRWAGLNDAKAIDINKIREQQLDRLADVLAEHLDMQKLQPILEA

Sequences:

>Translated_529_residues
MPSVNTMSSFTIDNTANINSPRRAAVLMVQGTTSDAGKSTLVAGICRLLARQGIKVAPFKPQNMALNSAVTIDGGEIGRA
QALQAVACYLEPHTDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGPKSKDYKAMALGAVLESFERLTAQYQTIVVEG
AGSPAEINLREGDIANMGFAEVVDCPVIIIADIDKGGVFAHLVGTLALLSESEQARVKGFVINRFRGDISLLQSGLDWLE
TYTKKPVLGVLPYLHDLHLDAEDALISSPTKGANSRLKVRVLVYPRISNHTDFDPLRLHPDIDFDYVSLQTQAASHSTHL
PAADVLILPGSKNVRADLAFLRQQGWDKDIATHLRYGGKVLGVCGGYQMLGQSIADPLGIEDSAGDTVGLGYLPLSTELK
AEKQLRRVSGELTLLGQKVAVKGYEIHCGESSYLIPRLEQTNVPLQLIPDSTSMSFADGLQSDDGQILGTYLHGLFDSPD
ACQLILRWAGLNDAKAIDINKIREQQLDRLADVLAEHLDMQKLQPILEA
>Mature_528_residues
PSVNTMSSFTIDNTANINSPRRAAVLMVQGTTSDAGKSTLVAGICRLLARQGIKVAPFKPQNMALNSAVTIDGGEIGRAQ
ALQAVACYLEPHTDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGPKSKDYKAMALGAVLESFERLTAQYQTIVVEGA
GSPAEINLREGDIANMGFAEVVDCPVIIIADIDKGGVFAHLVGTLALLSESEQARVKGFVINRFRGDISLLQSGLDWLET
YTKKPVLGVLPYLHDLHLDAEDALISSPTKGANSRLKVRVLVYPRISNHTDFDPLRLHPDIDFDYVSLQTQAASHSTHLP
AADVLILPGSKNVRADLAFLRQQGWDKDIATHLRYGGKVLGVCGGYQMLGQSIADPLGIEDSAGDTVGLGYLPLSTELKA
EKQLRRVSGELTLLGQKVAVKGYEIHCGESSYLIPRLEQTNVPLQLIPDSTSMSFADGLQSDDGQILGTYLHGLFDSPDA
CQLILRWAGLNDAKAIDINKIREQQLDRLADVLAEHLDMQKLQPILEA

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 57126; Mature: 56995

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSVNTMSSFTIDNTANINSPRRAAVLMVQGTTSDAGKSTLVAGICRLLARQGIKVAPFK
CCCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEECCCC
PQNMALNSAVTIDGGEIGRAQALQAVACYLEPHTDFNPILLKPSSDTGAQIIVHGKALTT
CCCCEECCEEEECCCCCCHHHHHHHHHEEECCCCCCCCEEEECCCCCCCEEEEECCEEEE
LEASAFFGPKSKDYKAMALGAVLESFERLTAQYQTIVVEGAGSPAEINLREGDIANMGFA
EEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCEEEECCCCCCCCCHH
EVVDCPVIIIADIDKGGVFAHLVGTLALLSESEQARVKGFVINRFRGDISLLQSGLDWLE
HHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHEEEEEHHHCHHHHHHHHHHHHH
TYTKKPVLGVLPYLHDLHLDAEDALISSPTKGANSRLKVRVLVYPRISNHTDFDPLRLHP
HHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCEEECC
DIDFDYVSLQTQAASHSTHLPAADVLILPGSKNVRADLAFLRQQGWDKDIATHLRYGGKV
CCCCEEEEEEEHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHCCCEE
LGVCGGYQMLGQSIADPLGIEDSAGDTVGLGYLPLSTELKAEKQLRRVSGELTLLGQKVA
EEECCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEECEEE
VKGYEIHCGESSYLIPRLEQTNVPLQLIPDSTSMSFADGLQSDDGQILGTYLHGLFDSPD
EEEEEEEECCCCEEECCCCCCCCCEEEECCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCH
ACQLILRWAGLNDAKAIDINKIREQQLDRLADVLAEHLDMQKLQPILEA
HHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC
>Mature Secondary Structure 
PSVNTMSSFTIDNTANINSPRRAAVLMVQGTTSDAGKSTLVAGICRLLARQGIKVAPFK
CCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEECCCC
PQNMALNSAVTIDGGEIGRAQALQAVACYLEPHTDFNPILLKPSSDTGAQIIVHGKALTT
CCCCEECCEEEECCCCCCHHHHHHHHHEEECCCCCCCCEEEECCCCCCCEEEEECCEEEE
LEASAFFGPKSKDYKAMALGAVLESFERLTAQYQTIVVEGAGSPAEINLREGDIANMGFA
EEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCEEEECCCCCCCCCHH
EVVDCPVIIIADIDKGGVFAHLVGTLALLSESEQARVKGFVINRFRGDISLLQSGLDWLE
HHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHEEEEEHHHCHHHHHHHHHHHHH
TYTKKPVLGVLPYLHDLHLDAEDALISSPTKGANSRLKVRVLVYPRISNHTDFDPLRLHP
HHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCEEECC
DIDFDYVSLQTQAASHSTHLPAADVLILPGSKNVRADLAFLRQQGWDKDIATHLRYGGKV
CCCCEEEEEEEHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHCCCEE
LGVCGGYQMLGQSIADPLGIEDSAGDTVGLGYLPLSTELKAEKQLRRVSGELTLLGQKVA
EEECCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEECEEE
VKGYEIHCGESSYLIPRLEQTNVPLQLIPDSTSMSFADGLQSDDGQILGTYLHGLFDSPD
EEEEEEEECCCCEEECCCCCCCCCEEEECCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCH
ACQLILRWAGLNDAKAIDINKIREQQLDRLADVLAEHLDMQKLQPILEA
HHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]