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The map label for this gene is cysN

Identifier: 120597733

GI number: 120597733

Start: 1030312

End: 1031754

Strand: Direct

Name: cysN

Synonym: Sputw3181_0903

Alternate gene names: 120597733

Gene position: 1030312-1031754 (Clockwise)

Preceding gene: 120597732

Following gene: 120597734

Centisome position: 21.88

GC content: 49.06

Gene sequence:

>1443_bases
ATGGACAAAGCGGTCAATAAAACCAATCTCATGGCGGCAGAAATTACCCTCCATGGTGTGAAAGAATATCTAGCCCTGCA
GCAACACAAAGGCTTACTGCGCTTTTTAACCTGTGGCAGTGTGGATGACGGCAAAAGTACCTTAATCGGCCGCCTGCTAC
ACGATAGCGCGCAGATCTATGAAGACCAATTGGCGAGCTTAAAAAGCGACAGCGCTAAGATGGGCACCACAGGTGAAGCG
ATTGACTTAGCTTTGCTGGTGGATGGTCTGCAAGCAGAGCGCGAGCAGGGCATTACAATCGATGTGGCTTACCGTTATTT
TTCAAGTGATAAGCGCAAGTTCATTATCGCCGATACGCCAGGGCATGAGCAGTACACTCGCAATATGGCGACAGGTGCAT
CGACCTGTGATTTAGCGGTGATTTTAGTCGATGCCCGCTACGGCGTGCAGACTCAGACTAAGCGCCATGCGTTTATCGCG
TCATTACTCGGTATTCGTCACTTTGTGGTTGCCGTGAATAAGATGGATTTGCTGGGGTTTGATGAGCAAGTATTCAATCG
TATTCGTGCCGATTTTACGGATTTCGTTAAAGATTTCGGTAATCTCGATATCCACTTCGTGCCCTTATCCGCGCTTAATG
GTGACAACGTGGTTGAGCGCAGTACCCAAAGTGACTGGTATCAAGGCGGAACACTGCTTGAACTGCTAGAAACCATTGAT
ACTCAACGCGAACTCAGTGCATTGCCCGTGCGTTTCCCTGTGCAATATGTGTCGCGTCCTCACTTAGATTTTCGCGGTTT
TGCGGGCACGTTAGCCTCTGGCATCATCAAGGTCGGCGACGAGTTAGTCGCGTTGCCATCGGGTAAATGCAGCAAGGTTG
AACGTATTGTCACCTTCGATGGTGATCTAGAGGAAGCGGTTGCAGGCCAGGCTGTGACTATCACGCTGGAAGATGAAATT
GATATTTCCCGTGGCGATTTACTGGCAAAACCCGACTCAGCTCCCGCCGTCGCTAACCTGATCAGCGCTGATATAGTGTG
GATGGATGAAAAGCCATTACAACTCGGTCAGTTATACGACATCAAAGTGGCCGGTAAGAAGACGCAAGCCTCTGTAACAG
CTATCGATTATGTGGTTGATGTTAACACCTTAGCGCGCAGTGCGACGGACACGCTTGGGTTAAACGCGATTGCCCGTGTG
ACCTTAGAACTGACCGAAGACATAGTATTTGATGCCTATTCGTTAGTGCGTGATACGGGCGGAATGATCTTAATCGACCG
TCTTACTAATGCGACGGTTGCCGCGGTGATGGTGGTGTCGGGTCAACATATTAACAAGCAAACAGCGTCACAATTTAGTG
CCTTTGAAGTCGAGTTTAATGCCTTAGTGCGTAAACACTTCCCCCATTGGCAAGCGATAGATATCACTCAGCTTGGAGCT
TAA

Upstream 100 bases:

>100_bases
CGTCAAGGCTACTTCTAATATCTTCAGGGTTAACCCTATTTCGCGAAGGTGGCGCTAGTCAGTGCGACCTTCAGCCAAGC
AGATCAAGAGTGAGAATAAG

Downstream 100 bases:

>100_bases
TTTGTGAGTGACTTGTGGCAATTAGCGATTATTTTGATAGGGCTTGTGGCTGGCTTAATTAGCGGCCGTATCAACCCAGC
GGTATTGTTTTTATTTGCAT

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MDKAVNKTNLMAAEITLHGVKEYLALQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKSDSAKMGTTGEA
IDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIA
SLLGIRHFVVAVNKMDLLGFDEQVFNRIRADFTDFVKDFGNLDIHFVPLSALNGDNVVERSTQSDWYQGGTLLELLETID
TQRELSALPVRFPVQYVSRPHLDFRGFAGTLASGIIKVGDELVALPSGKCSKVERIVTFDGDLEEAVAGQAVTITLEDEI
DISRGDLLAKPDSAPAVANLISADIVWMDEKPLQLGQLYDIKVAGKKTQASVTAIDYVVDVNTLARSATDTLGLNAIARV
TLELTEDIVFDAYSLVRDTGGMILIDRLTNATVAAVMVVSGQHINKQTASQFSAFEVEFNALVRKHFPHWQAIDITQLGA

Sequences:

>Translated_480_residues
MDKAVNKTNLMAAEITLHGVKEYLALQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKSDSAKMGTTGEA
IDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIA
SLLGIRHFVVAVNKMDLLGFDEQVFNRIRADFTDFVKDFGNLDIHFVPLSALNGDNVVERSTQSDWYQGGTLLELLETID
TQRELSALPVRFPVQYVSRPHLDFRGFAGTLASGIIKVGDELVALPSGKCSKVERIVTFDGDLEEAVAGQAVTITLEDEI
DISRGDLLAKPDSAPAVANLISADIVWMDEKPLQLGQLYDIKVAGKKTQASVTAIDYVVDVNTLARSATDTLGLNAIARV
TLELTEDIVFDAYSLVRDTGGMILIDRLTNATVAAVMVVSGQHINKQTASQFSAFEVEFNALVRKHFPHWQAIDITQLGA
>Mature_480_residues
MDKAVNKTNLMAAEITLHGVKEYLALQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKSDSAKMGTTGEA
IDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIA
SLLGIRHFVVAVNKMDLLGFDEQVFNRIRADFTDFVKDFGNLDIHFVPLSALNGDNVVERSTQSDWYQGGTLLELLETID
TQRELSALPVRFPVQYVSRPHLDFRGFAGTLASGIIKVGDELVALPSGKCSKVERIVTFDGDLEEAVAGQAVTITLEDEI
DISRGDLLAKPDSAPAVANLISADIVWMDEKPLQLGQLYDIKVAGKKTQASVTAIDYVVDVNTLARSATDTLGLNAIARV
TLELTEDIVFDAYSLVRDTGGMILIDRLTNATVAAVMVVSGQHINKQTASQFSAFEVEFNALVRKHFPHWQAIDITQLGA

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=431, Percent_Identity=29.4663573085847, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI5729864, Length=431, Percent_Identity=29.4663573085847, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI4503475, Length=336, Percent_Identity=29.7619047619048, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI4503471, Length=360, Percent_Identity=29.1666666666667, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI194018522, Length=418, Percent_Identity=27.7511961722488, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI194018520, Length=418, Percent_Identity=27.7511961722488, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI194097354, Length=418, Percent_Identity=27.7511961722488, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI46094014, Length=418, Percent_Identity=27.2727272727273, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI34147630, Length=315, Percent_Identity=30.4761904761905, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI157426893, Length=269, Percent_Identity=26.3940520446097, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1789108, Length=460, Percent_Identity=63.695652173913, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1790412, Length=314, Percent_Identity=28.6624203821656, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789737, Length=314, Percent_Identity=28.6624203821656, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI2367247, Length=256, Percent_Identity=28.125, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17552884, Length=360, Percent_Identity=31.1111111111111, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=360, Percent_Identity=31.1111111111111, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI32566629, Length=429, Percent_Identity=27.039627039627, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI115532067, Length=296, Percent_Identity=31.0810810810811, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI115532065, Length=296, Percent_Identity=31.0810810810811, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI32566303, Length=353, Percent_Identity=29.4617563739377, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.231884057971, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25141371, Length=205, Percent_Identity=29.2682926829268, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=352, Percent_Identity=25, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325337, Length=346, Percent_Identity=30.9248554913295, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6319594, Length=346, Percent_Identity=30.9248554913295, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322937, Length=408, Percent_Identity=29.9019607843137, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=315, Percent_Identity=29.2063492063492, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324761, Length=339, Percent_Identity=27.1386430678466, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323320, Length=178, Percent_Identity=32.0224719101124, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45550900, Length=433, Percent_Identity=26.0969976905312, Blast_Score=152, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24652838, Length=309, Percent_Identity=30.0970873786408, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17137572, Length=309, Percent_Identity=30.0970873786408, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45553807, Length=309, Percent_Identity=29.126213592233, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI45553816, Length=309, Percent_Identity=29.126213592233, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24651721, Length=309, Percent_Identity=29.126213592233, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI17864154, Length=309, Percent_Identity=29.126213592233, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI17137380, Length=419, Percent_Identity=26.0143198090692, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI281363316, Length=318, Percent_Identity=29.874213836478, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17864358, Length=318, Percent_Identity=29.874213836478, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19921738, Length=114, Percent_Identity=34.2105263157895, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 52523; Mature: 52523

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKAVNKTNLMAAEITLHGVKEYLALQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIY
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EDQLASLKSDSAKMGTTGEAIDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTP
HHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECC
GHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIASLLGIRHFVVAVNKMDLLGF
CCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCC
DEQVFNRIRADFTDFVKDFGNLDIHFVPLSALNGDNVVERSTQSDWYQGGTLLELLETID
CHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHCCCCCHHHCCCHHHHHHHHHC
TQRELSALPVRFPVQYVSRPHLDFRGFAGTLASGIIKVGDELVALPSGKCSKVERIVTFD
CHHHHHCCCEECCHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEEC
GDLEEAVAGQAVTITLEDEIDISRGDLLAKPDSAPAVANLISADIVWMDEKPLQLGQLYD
CCHHHHHCCCEEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHCEEEECCCCCCCCCEEE
IKVAGKKTQASVTAIDYVVDVNTLARSATDTLGLNAIARVTLELTEDIVFDAYSLVRDTG
EEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GMILIDRLTNATVAAVMVVSGQHINKQTASQFSAFEVEFNALVRKHFPHWQAIDITQLGA
CEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHEEEEHHHHHHHHHCCCCCEECCCCCCC
>Mature Secondary Structure
MDKAVNKTNLMAAEITLHGVKEYLALQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIY
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EDQLASLKSDSAKMGTTGEAIDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTP
HHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECC
GHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIASLLGIRHFVVAVNKMDLLGF
CCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCC
DEQVFNRIRADFTDFVKDFGNLDIHFVPLSALNGDNVVERSTQSDWYQGGTLLELLETID
CHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHCCCCCHHHCCCHHHHHHHHHC
TQRELSALPVRFPVQYVSRPHLDFRGFAGTLASGIIKVGDELVALPSGKCSKVERIVTFD
CHHHHHCCCEECCHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEEC
GDLEEAVAGQAVTITLEDEIDISRGDLLAKPDSAPAVANLISADIVWMDEKPLQLGQLYD
CCHHHHHCCCEEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHCEEEECCCCCCCCCEEE
IKVAGKKTQASVTAIDYVVDVNTLARSATDTLGLNAIARVTLELTEDIVFDAYSLVRDTG
EEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GMILIDRLTNATVAAVMVVSGQHINKQTASQFSAFEVEFNALVRKHFPHWQAIDITQLGA
CEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHEEEEHHHHHHHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]