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The map label for this gene is sspA

Identifier: 120597499

GI number: 120597499

Start: 735801

End: 736430

Strand: Direct

Name: sspA

Synonym: Sputw3181_0668

Alternate gene names: 120597499

Gene position: 735801-736430 (Clockwise)

Preceding gene: 120597498

Following gene: 120597500

Centisome position: 15.63

GC content: 45.56

Gene sequence:

>630_bases
ATGGCTGTTGCTGCCAACAAACGCTCTATCATGACACTGTTTTCAGGTGCTGATGATCTGTATAGCCATCAAGTACGTAT
CGTGTTAGCTGAGAAAGGGGTTACTGTTGATGTTTTACAGGTCGACCCTAATGAAATGCCTGAGGATTTGCTTGAAGTCA
ATCCTTATAATTCAGTGCCCACTTTGGTAGACCGTGAACTCGTTTTATACGAATCACGCATTATCATGGAATATTTGGAT
GAGCGTTTCCCTCATCCCCCTTTGATGCCAGTCTATCCGGTATCACGCGGCCAAAGCCGCTTGATGATGCATCGCATAGA
CACTGACTGGTATGCGCTGGTTGATCGCATTCGCAAAGGTGATCGTGCTGAAGCCGCTCGTAAAGAGTTGACTGAAAGTT
TGGTTGCTATTGCTCCTGTGTTTGGCGAAGTGCCCTATTTCATGAGTGAAGAATTTGGTTTAGCGGATTGCTATCTCGGC
CCACTATTATGGCGTTTGCCAGTATTAGGCATCGAGTTAGACAGCCGCGTAGCGAAAGACATCAAAGCCTATATGACACG
TATTTTTGAACGTGAGTCGTTTAAAGCTTCTTTGACTGAAGCTGAACGCGAAATGCGTATGGGCATGTAA

Upstream 100 bases:

>100_bases
CTAGGCACTAATAACGGGTAAAATGTAATATCCGCATAGTGTAAACGGGGGGCTTTGCCCCTCGTTTACTTTTGTTTTAT
TTCGATTCTGGAGGGTATCA

Downstream 100 bases:

>100_bases
TGAAATCTTTGACTCCAAATCGTCCTTATTTACTTAGGGCTTACTACGATTGGTTGATGGATAACCAATTAACGCCTCAC
GTTGTGGTTGATGCCTTTGT

Product: stringent starvation protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MAVAANKRSIMTLFSGADDLYSHQVRIVLAEKGVTVDVLQVDPNEMPEDLLEVNPYNSVPTLVDRELVLYESRIIMEYLD
ERFPHPPLMPVYPVSRGQSRLMMHRIDTDWYALVDRIRKGDRAEAARKELTESLVAIAPVFGEVPYFMSEEFGLADCYLG
PLLWRLPVLGIELDSRVAKDIKAYMTRIFERESFKASLTEAEREMRMGM

Sequences:

>Translated_209_residues
MAVAANKRSIMTLFSGADDLYSHQVRIVLAEKGVTVDVLQVDPNEMPEDLLEVNPYNSVPTLVDRELVLYESRIIMEYLD
ERFPHPPLMPVYPVSRGQSRLMMHRIDTDWYALVDRIRKGDRAEAARKELTESLVAIAPVFGEVPYFMSEEFGLADCYLG
PLLWRLPVLGIELDSRVAKDIKAYMTRIFERESFKASLTEAEREMRMGM
>Mature_208_residues
AVAANKRSIMTLFSGADDLYSHQVRIVLAEKGVTVDVLQVDPNEMPEDLLEVNPYNSVPTLVDRELVLYESRIIMEYLDE
RFPHPPLMPVYPVSRGQSRLMMHRIDTDWYALVDRIRKGDRAEAARKELTESLVAIAPVFGEVPYFMSEEFGLADCYLGP
LLWRLPVLGIELDSRVAKDIKAYMTRIFERESFKASLTEAEREMRMGM

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1789624, Length=210, Percent_Identity=70, Blast_Score=308, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI25144449, Length=177, Percent_Identity=26.5536723163842, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI21355779, Length=172, Percent_Identity=29.6511627906977, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24660979, Length=179, Percent_Identity=27.9329608938547, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 24005; Mature: 23874

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAANKRSIMTLFSGADDLYSHQVRIVLAEKGVTVDVLQVDPNEMPEDLLEVNPYNSVP
CCCCCCCHHHHHHHCCCHHHHHCEEEEEEECCCCEEEEEECCCCCCCHHHHHCCCCCCCC
TLVDRELVLYESRIIMEYLDERFPHPPLMPVYPVSRGQSRLMMHRIDTDWYALVDRIRKG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHCCCHHHHHHHHHHCC
DRAEAARKELTESLVAIAPVFGEVPYFMSEEFGLADCYLGPLLWRLPVLGIELDSRVAKD
CHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
IKAYMTRIFERESFKASLTEAEREMRMGM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVAANKRSIMTLFSGADDLYSHQVRIVLAEKGVTVDVLQVDPNEMPEDLLEVNPYNSVP
CCCCCCHHHHHHHCCCHHHHHCEEEEEEECCCCEEEEEECCCCCCCHHHHHCCCCCCCC
TLVDRELVLYESRIIMEYLDERFPHPPLMPVYPVSRGQSRLMMHRIDTDWYALVDRIRKG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHCCCHHHHHHHHHHCC
DRAEAARKELTESLVAIAPVFGEVPYFMSEEFGLADCYLGPLLWRLPVLGIELDSRVAKD
CHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
IKAYMTRIFERESFKASLTEAEREMRMGM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]