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The map label for this gene is acnB [H]

Identifier: 120597365

GI number: 120597365

Start: 581752

End: 584349

Strand: Direct

Name: acnB [H]

Synonym: Sputw3181_0534

Alternate gene names: 120597365

Gene position: 581752-584349 (Clockwise)

Preceding gene: 120597362

Following gene: 120597367

Centisome position: 12.36

GC content: 49.65

Gene sequence:

>2598_bases
GTGCTAGAAGCATATCGTAAACACGTCGCAGAACGTGCTGCAGAGGGCGTAGTCCCTAAGCCATTAGATGCACATCAAGT
GGCTGAACTGGTTAAATTGGTTCAAACCCCACCCGCAGGTGAAGAGGCTTTTATCCTTGACCTGCTCGAAAATCGAATTC
CCCCCGGTGTTGATGAAGCGGCTTATGTGAAAGCGGCCTTCTTAGATGCGGTTGCCAAAGGCTCTGTGACTTCACCCATC
CTATCTGCTGAGCGTGCTACCGAGTTATTAGGCACTATGCAAGGTGGCTATAACATTGAACCTCTGATCGCGCAGTTGGA
TAATCCAGCCCTCGCACCATTGGCCGTTAAAGCCCTAGCTCATACCCTACTGATGTTTGACTCTTTCCACGATGTGGTTG
AGAAAATGGAAGCAGGTAATGAGTTTGCTAAGCAAGTGGTTGAAGCTTGGGCGAATGCAGACTGGTACTTAAGCCGTCCA
AAATTAGCGGACAAAGTGACCCTGACAGTATTCAAAGTCTCTGGTGAAACCAACACCGATGACTTGTCTCCAGCGCCCGA
TGCTTGGTCACGTCCTGATATCCCATTGCACGCGTTAGCGATGCTGAAAAACGCCCGCGACGGTATCGAGCCTGATGTAC
CTGGTAGCGTAGGTCCAATCAAGAAATTAGACGAACTGAAAACCAAAGGTTTCCCATTGGTGTATGTGGGTGACGTTGTG
GGTACGGGTTCATCGCGTAAGTCAGCGACTAACTCAGTGCTGTGGTTTATGGGCGATGATATTCCATTCGTCCCTAACAA
ACGTGCTGGTGGTTTCTGTTTAGGCGGTAAAATTGCGCCGATTTTCTTCAACACGATGGAAGATGCGGGCGCCCTGCCAA
TTGAGCTTGACGTGAGCAAAATGGAAATGGGCGATGTTATCGACATTTACCCCTATGCTGGCATTGTTAAGCGCCACGGC
AGCGATGAAGTCATTTCAGAATTCAGCTTAAAAACAGAAGTCTTGTTAGACGAAGTCCGTGCAGGTGGCCGTATTCCACT
GATCATTGGTCGCGGCTTAACTGACAAGGCTCGTGAAGTCTTAGGCTTACCAGCATCTGATGTATTCGTACGTCCACAGG
ATGTCGCCGATACAGGTAAAGGTTACACGCTGGCGCAGAAGATGGTCGGTAAAGCATGCGGCGTAGCGGGTGTGCGTCCT
GGCCAATACTGCGAACCTAAGATGACGTCTGTGGGCTCACAGGATACTACAGGTCCTATGACCCGCGACGAGCTAAAAGA
TTTAGCTTGTTTAGGTTTCAGTGCCGATTTGACGATGCAGTCATTTTGCCACACAGCGGCCTATCCAAAGCCAGTTGATG
TTAACACACATCATACGCTGCCAGACTTCATTATGAACCGTGGTGGTGTATCACTGCGCCCAGGTGACGGTGTTATCCAC
TCATGGTTAAACCGTATGCTGCTACCAGATACAGTCGGTACTGGTGGTGACTCACATACGCGTTTCCCTATCGGCATTTC
ATTCCCAGCAGGTTCTGGCTTAGTGGCGTTTGCTGCCGCGACTGGCGTTATGCCGCTCGACATGCCTGAATCCGTATTAG
TGCGCTTCAAGGGCCAAATGCAACCGGGTATCACACTGCGTGATCTGGTGCATGCTATCCCGCTTAAAGCCATTGAGCTC
GGGATGCTGACCGTTGAGAAGAAAGGTAAAGTGAACATCTTCTCTGGTCGTGTACTGGAAATCGAAGGCTTAGAATCCCT
GAAAGTTGAACAGGCATTCGAACTGTCTGATGCATCAGCAGAACGCTCTGCAGCGGGTTGTACTATCAAGTTAGACAAAG
AGCCAATCATCGAATACCTGAACTCGAACATCACTATGCTGAAGTGGATGATCGCCGAAGGCTACGGTGATCGTCGTACG
ATAGAGCGTCGTATCAAAGGCATGCAAGAGTGGTTAGCGAATCCTGAACTGATGAGCGCCGATAAAGATGCCGAGTATGC
TGCAGTAATCGAAATCGATCTGAACGAGATCAAAGAGCCAATCCTGTGTGCGCCTAACGATCCAGACGATGCAGTATTAC
TGTCATCTGTTGCGCAAACGAAGATTGACGAAGTGTTTGTCGGTTCTTGTATGACTAACATTGGTCACTTCCGTGCGACT
GGTAAGATGTTAGACAAGTTCGCTAAGACATTGCCAACGCGTCTGTGGATTGCGCCACCGACTAAGATGGACAGAGATCA
ACTGACCGAAGAAGGCTATTACGCGATTTTTGGTCGCGTCGGTGCGCGTATCGAGATCCCTGGCTGTTCACTGTGTATGG
GTAACCAAGCACGTGTGGCCGAAGGTGCAACCGTGGTGTCGACTTCTACCCGTAACTTCCCTAACCGTTTAGGTACAGGC
GCGAATGTTTACTTAGCTTCTGCGGAATTAGCGGCAGTTGCAGCGCTATTGGGTCGTCTGCCAACGGTTGAAGAATACCA
AGAATACGCGAAAGAGTTGGATGCTACGGCGGCTGACACTTACCGTTACTTGAACTTCGACCAAATCGATTCTTACACGA
AAAAAGCAGCGGAAGTGATCTTCCAGACTGCAGTGTAA

Upstream 100 bases:

>100_bases
TGCTGGCCGGAAATACGGGTCGATCATGTCGGACTGTCAGCTTCAATAAAGAAGACGTGCAGTTTCGTTGTTAGAGCGCC
AAACAAAGAGGAATGTTGCC

Downstream 100 bases:

>100_bases
TTAATTGATATAAAATAAAAAAGCCAGCTTACGCTGGCTTTTTTATTGTCTGAAAAAGAGCATTCGTTGTGGGATGAATT
ATACCTGTTGGGTTTGCTCT

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 865; Mature: 865

Protein sequence:

>865_residues
MLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQTPPAGEEAFILDLLENRIPPGVDEAAYVKAAFLDAVAKGSVTSPI
LSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAVKALAHTLLMFDSFHDVVEKMEAGNEFAKQVVEAWANADWYLSRP
KLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVPGSVGPIKKLDELKTKGFPLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSKMEMGDVIDIYPYAGIVKRHG
SDEVISEFSLKTEVLLDEVRAGGRIPLIIGRGLTDKAREVLGLPASDVFVRPQDVADTGKGYTLAQKMVGKACGVAGVRP
GQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGQMQPGITLRDLVHAIPLKAIEL
GMLTVEKKGKVNIFSGRVLEIEGLESLKVEQAFELSDASAERSAAGCTIKLDKEPIIEYLNSNITMLKWMIAEGYGDRRT
IERRIKGMQEWLANPELMSADKDAEYAAVIEIDLNEIKEPILCAPNDPDDAVLLSSVAQTKIDEVFVGSCMTNIGHFRAT
GKMLDKFAKTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQARVAEGATVVSTSTRNFPNRLGTG
ANVYLASAELAAVAALLGRLPTVEEYQEYAKELDATAADTYRYLNFDQIDSYTKKAAEVIFQTAV

Sequences:

>Translated_865_residues
MLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQTPPAGEEAFILDLLENRIPPGVDEAAYVKAAFLDAVAKGSVTSPI
LSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAVKALAHTLLMFDSFHDVVEKMEAGNEFAKQVVEAWANADWYLSRP
KLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVPGSVGPIKKLDELKTKGFPLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSKMEMGDVIDIYPYAGIVKRHG
SDEVISEFSLKTEVLLDEVRAGGRIPLIIGRGLTDKAREVLGLPASDVFVRPQDVADTGKGYTLAQKMVGKACGVAGVRP
GQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGQMQPGITLRDLVHAIPLKAIEL
GMLTVEKKGKVNIFSGRVLEIEGLESLKVEQAFELSDASAERSAAGCTIKLDKEPIIEYLNSNITMLKWMIAEGYGDRRT
IERRIKGMQEWLANPELMSADKDAEYAAVIEIDLNEIKEPILCAPNDPDDAVLLSSVAQTKIDEVFVGSCMTNIGHFRAT
GKMLDKFAKTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQARVAEGATVVSTSTRNFPNRLGTG
ANVYLASAELAAVAALLGRLPTVEEYQEYAKELDATAADTYRYLNFDQIDSYTKKAAEVIFQTAV
>Mature_865_residues
MLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQTPPAGEEAFILDLLENRIPPGVDEAAYVKAAFLDAVAKGSVTSPI
LSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAVKALAHTLLMFDSFHDVVEKMEAGNEFAKQVVEAWANADWYLSRP
KLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVPGSVGPIKKLDELKTKGFPLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSKMEMGDVIDIYPYAGIVKRHG
SDEVISEFSLKTEVLLDEVRAGGRIPLIIGRGLTDKAREVLGLPASDVFVRPQDVADTGKGYTLAQKMVGKACGVAGVRP
GQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGQMQPGITLRDLVHAIPLKAIEL
GMLTVEKKGKVNIFSGRVLEIEGLESLKVEQAFELSDASAERSAAGCTIKLDKEPIIEYLNSNITMLKWMIAEGYGDRRT
IERRIKGMQEWLANPELMSADKDAEYAAVIEIDLNEIKEPILCAPNDPDDAVLLSSVAQTKIDEVFVGSCMTNIGHFRAT
GKMLDKFAKTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQARVAEGATVVSTSTRNFPNRLGTG
ANVYLASAELAAVAALLGRLPTVEEYQEYAKELDATAADTYRYLNFDQIDSYTKKAAEVIFQTAV

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=865, Percent_Identity=80.8092485549133, Blast_Score=1437, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=393, Percent_Identity=24.9363867684478, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323335, Length=381, Percent_Identity=25.1968503937008, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 93562; Mature: 93562

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQTPPAGEEAFILDLLENRIPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHH
AYVKAAFLDAVAKGSVTSPILSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAVKALA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHH
HTLLMFDSFHDVVEKMEAGNEFAKQVVEAWANADWYLSRPKLADKVTLTVFKVSGETNTD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEEEECCCCCCC
DLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVPGSVGPIKKLDELKTKGFPLVYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCEE
GTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEHEEECHHCCCCCEEEEEHHH
MEMGDVIDIYPYAGIVKRHGSDEVISEFSLKTEVLLDEVRAGGRIPLIIGRGLTDKAREV
HCCCCEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LGLPASDVFVRPQDVADTGKGYTLAQKMVGKACGVAGVRPGQYCEPKMTSVGSQDTTGPM
HCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCC
TRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH
CHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCCHHH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGQM
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEHHHHCCCCCCCCCHHHHHHCCCCC
QPGITLRDLVHAIPLKAIELGMLTVEKKGKVNIFSGRVLEIEGLESLKVEQAFELSDASA
CCCCHHHHHHHHCCHHHHHCCEEEEECCCCEEEECCCEEEECCCCHHHHHHHHHCCCCCC
ERSAAGCTIKLDKEPIIEYLNSNITMLKWMIAEGYGDRRTIERRIKGMQEWLANPELMSA
HHCCCCCEEEECHHHHHHHHCCCHHEEHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC
DKDAEYAAVIEIDLNEIKEPILCAPNDPDDAVLLSSVAQTKIDEVFVGSCMTNIGHFRAT
CCCCCEEEEEEEEHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKMLDKFAKTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQARVA
HHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCEEEEECCCCEEECCCCCEECCCCCCCC
EGATVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPTVEEYQEYAKELDATAADT
CCCEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
YRYLNFDQIDSYTKKAAEVIFQTAV
HHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQTPPAGEEAFILDLLENRIPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHH
AYVKAAFLDAVAKGSVTSPILSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAVKALA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHH
HTLLMFDSFHDVVEKMEAGNEFAKQVVEAWANADWYLSRPKLADKVTLTVFKVSGETNTD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEEEECCCCCCC
DLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVPGSVGPIKKLDELKTKGFPLVYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCEE
GTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEHEEECHHCCCCCEEEEEHHH
MEMGDVIDIYPYAGIVKRHGSDEVISEFSLKTEVLLDEVRAGGRIPLIIGRGLTDKAREV
HCCCCEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LGLPASDVFVRPQDVADTGKGYTLAQKMVGKACGVAGVRPGQYCEPKMTSVGSQDTTGPM
HCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCC
TRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH
CHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCCHHH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGQM
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEHHHHCCCCCCCCCHHHHHHCCCCC
QPGITLRDLVHAIPLKAIELGMLTVEKKGKVNIFSGRVLEIEGLESLKVEQAFELSDASA
CCCCHHHHHHHHCCHHHHHCCEEEEECCCCEEEECCCEEEECCCCHHHHHHHHHCCCCCC
ERSAAGCTIKLDKEPIIEYLNSNITMLKWMIAEGYGDRRTIERRIKGMQEWLANPELMSA
HHCCCCCEEEECHHHHHHHHCCCHHEEHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC
DKDAEYAAVIEIDLNEIKEPILCAPNDPDDAVLLSSVAQTKIDEVFVGSCMTNIGHFRAT
CCCCCEEEEEEEEHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKMLDKFAKTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQARVA
HHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCEEEEECCCCEEECCCCCEECCCCCCCC
EGATVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPTVEEYQEYAKELDATAADT
CCCEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
YRYLNFDQIDSYTKKAAEVIFQTAV
HHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]