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The map label for this gene is lpdA [H]

Identifier: 120597359

GI number: 120597359

Start: 569658

End: 571085

Strand: Direct

Name: lpdA [H]

Synonym: Sputw3181_0528

Alternate gene names: 120597359

Gene position: 569658-571085 (Clockwise)

Preceding gene: 120597358

Following gene: 120597360

Centisome position: 12.1

GC content: 47.06

Gene sequence:

>1428_bases
ATGAGTAACGAAATCAAAACTCAGGTAGTGGTATTAGGTGCAGGTCCTGCAGGTTATTCTGCGGCTTTCCGTGCTGCTGA
CTTAGGTCTGGAAACCATCATAGTTGAACGTTTTAGCACTTTAGGTGGCGTGTGTCTTAACGTGGGTTGTATCCCATCTA
AAGCCTTATTGCACGTTGCTAAAGTTATCGAAGAAGCCAAAGCGGTTGCCGCTCATGGTGTGGTCTTCGGCGAGCCAACT
ATCGATTTAGATAAGTTACGTAGCTTTAAAGAAAAAGTCATTGGCCAATTAACTGGCGGCTTGGGCGGCATGTCCAAAAT
GCGTAAAGTTAACGTGGTTAACGGTTTTGGTAAATTCACTGGCCCTAACACGCTAGAAGTGACTGCTGAAGATGGCACTG
TGACTAAAGTGACTTTCGAGCAAGCGATCATTGCTGCAGGTTCTCGCCCAATCAAACTGCCATTTATTCCCCACGAAGAC
CCACGTATTTGGGATTCGACTGACGCATTAGAACTGAAAGCAGTTCCAGGCAAGTTGTTAGTGATGGGCGGCGGTATTAT
CGGTTTAGAAATGGGTACTGTGTATTCATCTTTAGGCAGTGAAATCGACGTGGTTGAAATGTTCGACCAAGTGATCCCTG
CGGCTGATAAAGATGTCGTTCGCGTATTCACTAAGCAAATCAAGAAGAAATTCAACCTGATCCTCGAAACTAAAGTCACC
GCGGTAGAAGCCCGTGAAGACGGTATTTACGTTTCCATGGAAGGTAAGAGCGCGCCAGCTGAACCTGTACGTTACGATGC
GGTATTAGTCGCTATCGGCCGTACACCTAATGGCAAGCTGATAGACGCTGACAAAGCGGGTGTTAAGATCGACGAGCGTG
GCTTTATCAACGTAGACAAGCAATTACGTACTAACGTACCGCACATCTACGCTATCGGTGATATCGTTGGTCAACCAATG
TTGGCTCACAAAGGCGTGCACGAAGGCCACGTAGCGGCTGAAGTGATCGCGGGTATGAAGCATTACTTTGATCCAAAAGT
GATCCCATCAATTGCTTACACAGACCCTGAAGTGGCCTGGGTTGGTTTGACTGAGAAAGAAGCGAAAGAGCAAGGTATTG
CTTACGAAACTGCGACTTTCCCATGGGCAGCAAGTGGTCGTGCGATTGCGTCAGATTGCAGCGAAGGTATGACTAAGCTG
ATTTTCGATAAAGATACTCACCGCGTTATCGGTGGTGCAATTGTCGGTGTGAACGGTGGCGAACTGTTAGGCGAAATCGG
TCTAGCGATTGAAATGGGTTGTGATGCTGAAGATTTAGCATTAACGATTCACGCTCACCCAACGCTGCACGAATCAGTAG
GCCTAGCGGCTGAAATGTACGAAGGTTCGATTACTGACTTGCCAAATCCAAAGGCAAAGAAAAAGTAA

Upstream 100 bases:

>100_bases
GTATCAAACACAGGTTAAAATGCGCCCACCTTACGCGCTGGCGCATTTTCGGTTGGCAGGATAACTCCTCCAACGGATTG
AATGAGAATTAGAGGAAAAC

Downstream 100 bases:

>100_bases
ATTGACCTTCATTTTTGAAAAAGCGCCCTTCAAGGCGCTTTTTTATTGCGTCGCGTAAAATGTAACTTTAATGTAAACGA
GCGGGTGAATTGATAGAGTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETIIVERFSTLGGVCLNVGCIPSKALLHVAKVIEEAKAVAAHGVVFGEPT
IDLDKLRSFKEKVIGQLTGGLGGMSKMRKVNVVNGFGKFTGPNTLEVTAEDGTVTKVTFEQAIIAAGSRPIKLPFIPHED
PRIWDSTDALELKAVPGKLLVMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
AVEAREDGIYVSMEGKSAPAEPVRYDAVLVAIGRTPNGKLIDADKAGVKIDERGFINVDKQLRTNVPHIYAIGDIVGQPM
LAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAWVGLTEKEAKEQGIAYETATFPWAASGRAIASDCSEGMTKL
IFDKDTHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEMYEGSITDLPNPKAKKK

Sequences:

>Translated_475_residues
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETIIVERFSTLGGVCLNVGCIPSKALLHVAKVIEEAKAVAAHGVVFGEPT
IDLDKLRSFKEKVIGQLTGGLGGMSKMRKVNVVNGFGKFTGPNTLEVTAEDGTVTKVTFEQAIIAAGSRPIKLPFIPHED
PRIWDSTDALELKAVPGKLLVMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
AVEAREDGIYVSMEGKSAPAEPVRYDAVLVAIGRTPNGKLIDADKAGVKIDERGFINVDKQLRTNVPHIYAIGDIVGQPM
LAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAWVGLTEKEAKEQGIAYETATFPWAASGRAIASDCSEGMTKL
IFDKDTHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEMYEGSITDLPNPKAKKK
>Mature_474_residues
SNEIKTQVVVLGAGPAGYSAAFRAADLGLETIIVERFSTLGGVCLNVGCIPSKALLHVAKVIEEAKAVAAHGVVFGEPTI
DLDKLRSFKEKVIGQLTGGLGGMSKMRKVNVVNGFGKFTGPNTLEVTAEDGTVTKVTFEQAIIAAGSRPIKLPFIPHEDP
RIWDSTDALELKAVPGKLLVMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVTA
VEAREDGIYVSMEGKSAPAEPVRYDAVLVAIGRTPNGKLIDADKAGVKIDERGFINVDKQLRTNVPHIYAIGDIVGQPML
AHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAWVGLTEKEAKEQGIAYETATFPWAASGRAIASDCSEGMTKLI
FDKDTHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEMYEGSITDLPNPKAKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=42.2907488986784, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI50301238, Length=454, Percent_Identity=27.9735682819383, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI33519430, Length=429, Percent_Identity=30.0699300699301, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519428, Length=429, Percent_Identity=30.0699300699301, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519426, Length=429, Percent_Identity=30.0699300699301, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI148277071, Length=429, Percent_Identity=30.0699300699301, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI148277065, Length=429, Percent_Identity=30.0699300699301, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI291045266, Length=431, Percent_Identity=29.4663573085847, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI22035672, Length=458, Percent_Identity=27.5109170305677, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI291045268, Length=425, Percent_Identity=28, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI1786307, Length=475, Percent_Identity=85.6842105263158, Blast_Score=836, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=468, Percent_Identity=27.1367521367521, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87081717, Length=456, Percent_Identity=27.4122807017544, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789915, Length=443, Percent_Identity=29.3453724604966, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI32565766, Length=454, Percent_Identity=40.0881057268723, Blast_Score=335, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=29.1139240506329, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71982272, Length=450, Percent_Identity=26.8888888888889, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=444, Percent_Identity=26.8018018018018, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=444, Percent_Identity=26.8018018018018, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=40.7894736842105, Blast_Score=307, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6325240, Length=468, Percent_Identity=27.3504273504274, Blast_Score=176, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6325166, Length=454, Percent_Identity=26.6519823788546, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=40.7002188183807, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24640553, Length=467, Percent_Identity=30.8351177730193, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640549, Length=467, Percent_Identity=30.8351177730193, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640551, Length=467, Percent_Identity=30.8351177730193, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17737741, Length=477, Percent_Identity=27.0440251572327, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50512; Mature: 50381

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETIIVERFSTLGGVCLNVGCIPSKALLHVA
CCCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAVAAHGVVFGEPTIDLDKLRSFKEKVIGQLTGGLGGMSKMRKVNVVNGFGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHEEEEEECCCCCC
GPNTLEVTAEDGTVTKVTFEQAIIAAGSRPIKLPFIPHEDPRIWDSTDALELKAVPGKLL
CCCEEEEEECCCEEEEEEHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEEEECCCEEE
VMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
EEECCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEE
AVEAREDGIYVSMEGKSAPAEPVRYDAVLVAIGRTPNGKLIDADKAGVKIDERGFINVDK
EEEECCCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCCEEECCCCCCEECCCCCEEECH
QLRTNVPHIYAIGDIVGQPMLAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAW
HHHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEE
VGLTEKEAKEQGIAYETATFPWAASGRAIASDCSEGMTKLIFDKDTHRVIGGAIVGVNGG
EECCHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCEEEECCCCCEEECEEEEECCCH
ELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEMYEGSITDLPNPKAKKK
HHHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNEIKTQVVVLGAGPAGYSAAFRAADLGLETIIVERFSTLGGVCLNVGCIPSKALLHVA
CCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAVAAHGVVFGEPTIDLDKLRSFKEKVIGQLTGGLGGMSKMRKVNVVNGFGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHEEEEEECCCCCC
GPNTLEVTAEDGTVTKVTFEQAIIAAGSRPIKLPFIPHEDPRIWDSTDALELKAVPGKLL
CCCEEEEEECCCEEEEEEHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEEEECCCEEE
VMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
EEECCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEE
AVEAREDGIYVSMEGKSAPAEPVRYDAVLVAIGRTPNGKLIDADKAGVKIDERGFINVDK
EEEECCCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCCEEECCCCCCEECCCCCEEECH
QLRTNVPHIYAIGDIVGQPMLAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAW
HHHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEE
VGLTEKEAKEQGIAYETATFPWAASGRAIASDCSEGMTKLIFDKDTHRVIGGAIVGVNGG
EECCHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCEEEECCCCCEEECEEEEECCCH
ELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEMYEGSITDLPNPKAKKK
HHHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]