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The map label for this gene is aceF [H]

Identifier: 120597358

GI number: 120597358

Start: 567501

End: 569510

Strand: Direct

Name: aceF [H]

Synonym: Sputw3181_0527

Alternate gene names: 120597358

Gene position: 567501-569510 (Clockwise)

Preceding gene: 120597357

Following gene: 120597359

Centisome position: 12.05

GC content: 51.09

Gene sequence:

>2010_bases
ATGGCTGAATTAAAAGAAGTTTTTGTTCCTGATATCGGCGGCGACGAAGTGCAAGTGATCGAAATTTGTGCCGCTGTGGG
TGATACCCTCGCGGCCGATGAATCGATTATTACCGTTGAAAGCGACAAGGCGACTATGGATATTCCTGCGCCTTTCGCCG
GTGTGTTAGCCGAGTTAAAAGTGGCTGTGGGTGATAAAGTCTCTGAAGGTACTTTGATTGCTATGATGCAAGCAGCGGGC
GCGGCAGCTGCCGATCCTGCTCCAGTTGCGGCACCATCATCCGCGCCAGCAGCTGCACCGGTTCAAGCGGCTCCTGCTCC
CGCAGTACCAGCAGCGACATCGACTCAAGCTGTTGAGACAAAAGTCGTTGAAGTGGCTGTGCCAGATATCGGTGGCGACA
CTGATGTCTCGGTTATCGAAGTGCTGGTTGCCGTCGGTGATAAGATTGAGGTTGATAGCGGTTTAATTACTCTCGAAACT
GACAAAGCGACCATGGATGTACCTTCACCTTTTGCTGGTGTGGTAAAAGAAGTCAAAGTAGCCGTTGGCGATAAAGTGTC
GGAAGGCTCTTTAGTCATCATGCTTGAAGTGGGTGGCGCAGCTCCTGCTGTGGCAGCAAGTGCGCCAACCGTTGCCGCTC
AGGCGGCTCCTGCCGCTACGGTAGCGCCAGTGGCTCCGGCTTCTGCAACTCCAACTGCAAGTGTGGTTACGGTAAAAGAA
ATTCAAGTGCCAGATATTGGCGATGCAAGCAATGTCGATGTGATTGAAGTGCTAGTGTCTGTGGGCGATATGATCACGGC
CGACCAAGGTTTAATAACCCTTGAAACTGACAAAGCCACCATGGAAGTGCCAGCGCCATTTGCCGGCAAACTATTGTCGT
TAACCGTTAAAGTGGGTGATAAGGTTTCTCAAGGTAGCGTGATAGCTACCGTTGAAACGACTGCTGTTGGAGCCGCTGCG
CCTGCGCCAGTTGCTCAAGCGCCAGCAGTGCAAGAAGTGGCGCCAGTTGCTGCTCAAGCACCTGCAAGCCGTCCGCCAGT
GCCGCATCATCCAAGTGCAGGTGCGCCTGTGTCGACTGGTGCGGTACATGCATCGCCTGCGGTACGCCGTTTAGCCCGTG
AATTTGGTGTGGATCTCACCCAAGTCACTGGTTCTGGCCGTAAAGGTCGCATTATGAAAGAAGACGTTCAGGCGTATGTG
AAATACGAACTGTCGCGTCCGAAAGCAACTGCGGCAACGTCAGTCGGCGCTGGCAATGGCGGTGGTCTGCAAGTGATTGC
AGCACCTAAAGTGGATTTCAGTAAGTTTGGTGAAGTGGAAGAAATTCCATTAAGCCGTATCCAGAAGATCTCTGGCCCTA
ACTTACACCGCAACTGGGTGACTATTCCGCATGTGACTCAGTTCGATGAAGCTGATATCACTGAAATGGAAGAGTTCCGT
AAGCAGCAAAACGACGCAGCGGCGAAGAAGAAAGCCGATTATAAGATCACGCCTTTAGTCTTTATGATGAAAGCGGTGGC
TAAGACGTTGCAACAGTTCCCAGTGTTCAACTCGAGCTTAAGCTCTGATGGCGAATCACTGATCCAGAAGAAGTATTTCC
ACATCGGTGTGGCGGTGGATACGCCAAACGGTTTGGTTGTGCCAGTCGTGCGTGACGTGGATAAGAAAGGCATTATCGAG
TTATCTCGTGAACTGGCTGATATCTCTATCCGTGCCCGTGATGGCAAGCTGAAATCTGCTGATATGCAGGGCAGCTGTTT
CACCATTTCAAGTTTAGGTGGTATTGGCGGTACGGCGTTTACGCCTATCGTTAACTACCCAGATGTGGCGATTTTAGGTG
TGTCTAAATCTGAAATTAAGCCTAAGTGGAATGGTAAAGAGTTCGAGCCTAAATTGATGTTGCCACTGTCGCTATCATAC
GATCACCGCGTGATCGATGGTGCCATGGCTGCACGCTTTAGCGTGACCCTGTCAGGAATTCTGTCCGATATTCGTACTTT
GATTCTGTAA

Upstream 100 bases:

>100_bases
TCGTAAAGAGCTACCTGTCGATGTGTTAGCAAACGCCATTAAAGAATATGGTATCGACGCTGACAAGATCAATCCACAGT
ACGCGTAAGAGGCAATGAAA

Downstream 100 bases:

>100_bases
ACATATAAGGCTGCTCAACTTGAGCAGCCTTTTGTTTATTGTGATCAGTATCAAACACAGGTTAAAATGCGCCCACCTTA
CGCGCTGGCGCATTTTCGGT

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 669; Mature: 668

Protein sequence:

>669_residues
MAELKEVFVPDIGGDEVQVIEICAAVGDTLAADESIITVESDKATMDIPAPFAGVLAELKVAVGDKVSEGTLIAMMQAAG
AAAADPAPVAAPSSAPAAAPVQAAPAPAVPAATSTQAVETKVVEVAVPDIGGDTDVSVIEVLVAVGDKIEVDSGLITLET
DKATMDVPSPFAGVVKEVKVAVGDKVSEGSLVIMLEVGGAAPAVAASAPTVAAQAAPAATVAPVAPASATPTASVVTVKE
IQVPDIGDASNVDVIEVLVSVGDMITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSVIATVETTAVGAAA
PAPVAQAPAVQEVAPVAAQAPASRPPVPHHPSAGAPVSTGAVHASPAVRRLAREFGVDLTQVTGSGRKGRIMKEDVQAYV
KYELSRPKATAATSVGAGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFR
KQQNDAAAKKKADYKITPLVFMMKAVAKTLQQFPVFNSSLSSDGESLIQKKYFHIGVAVDTPNGLVVPVVRDVDKKGIIE
LSRELADISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSY
DHRVIDGAMAARFSVTLSGILSDIRTLIL

Sequences:

>Translated_669_residues
MAELKEVFVPDIGGDEVQVIEICAAVGDTLAADESIITVESDKATMDIPAPFAGVLAELKVAVGDKVSEGTLIAMMQAAG
AAAADPAPVAAPSSAPAAAPVQAAPAPAVPAATSTQAVETKVVEVAVPDIGGDTDVSVIEVLVAVGDKIEVDSGLITLET
DKATMDVPSPFAGVVKEVKVAVGDKVSEGSLVIMLEVGGAAPAVAASAPTVAAQAAPAATVAPVAPASATPTASVVTVKE
IQVPDIGDASNVDVIEVLVSVGDMITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSVIATVETTAVGAAA
PAPVAQAPAVQEVAPVAAQAPASRPPVPHHPSAGAPVSTGAVHASPAVRRLAREFGVDLTQVTGSGRKGRIMKEDVQAYV
KYELSRPKATAATSVGAGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFR
KQQNDAAAKKKADYKITPLVFMMKAVAKTLQQFPVFNSSLSSDGESLIQKKYFHIGVAVDTPNGLVVPVVRDVDKKGIIE
LSRELADISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSY
DHRVIDGAMAARFSVTLSGILSDIRTLIL
>Mature_668_residues
AELKEVFVPDIGGDEVQVIEICAAVGDTLAADESIITVESDKATMDIPAPFAGVLAELKVAVGDKVSEGTLIAMMQAAGA
AAADPAPVAAPSSAPAAAPVQAAPAPAVPAATSTQAVETKVVEVAVPDIGGDTDVSVIEVLVAVGDKIEVDSGLITLETD
KATMDVPSPFAGVVKEVKVAVGDKVSEGSLVIMLEVGGAAPAVAASAPTVAAQAAPAATVAPVAPASATPTASVVTVKEI
QVPDIGDASNVDVIEVLVSVGDMITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSVIATVETTAVGAAAP
APVAQAPAVQEVAPVAAQAPASRPPVPHHPSAGAPVSTGAVHASPAVRRLAREFGVDLTQVTGSGRKGRIMKEDVQAYVK
YELSRPKATAATSVGAGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRK
QQNDAAAKKKADYKITPLVFMMKAVAKTLQQFPVFNSSLSSDGESLIQKKYFHIGVAVDTPNGLVVPVVRDVDKKGIIEL
SRELADISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYD
HRVIDGAMAARFSVTLSGILSDIRTLIL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=439, Percent_Identity=28.0182232346241, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI31711992, Length=328, Percent_Identity=32.6219512195122, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=34.3612334801762, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI203098816, Length=442, Percent_Identity=26.9230769230769, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI203098753, Length=442, Percent_Identity=26.6968325791855, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI260898739, Length=147, Percent_Identity=36.0544217687075, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1786305, Length=665, Percent_Identity=54.8872180451128, Blast_Score=626, Evalue=1e-180,
Organism=Escherichia coli, GI1786946, Length=412, Percent_Identity=30.3398058252427, Blast_Score=175, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=430, Percent_Identity=27.4418604651163, Blast_Score=156, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17560088, Length=438, Percent_Identity=30.365296803653, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25146366, Length=215, Percent_Identity=39.0697674418605, Blast_Score=129, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=30.4487179487179, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=432, Percent_Identity=28.9351851851852, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=431, Percent_Identity=26.4501160092807, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI18859875, Length=445, Percent_Identity=29.438202247191, Blast_Score=163, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24645909, Length=214, Percent_Identity=35.5140186915888, Blast_Score=119, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24582497, Length=316, Percent_Identity=28.4810126582279, Blast_Score=109, Evalue=8e-24,
Organism=Drosophila melanogaster, GI20129315, Length=316, Percent_Identity=28.4810126582279, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 68780; Mature: 68649

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELKEVFVPDIGGDEVQVIEICAAVGDTLAADESIITVESDKATMDIPAPFAGVLAELK
CCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCEEECCCCHHHHHHHHH
VAVGDKVSEGTLIAMMQAAGAAAADPAPVAAPSSAPAAAPVQAAPAPAVPAATSTQAVET
HHHCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHE
KVVEVAVPDIGGDTDVSVIEVLVAVGDKIEVDSGLITLETDKATMDVPSPFAGVVKEVKV
EEEEEEECCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHH
AVGDKVSEGSLVIMLEVGGAAPAVAASAPTVAAQAAPAATVAPVAPASATPTASVVTVKE
HHCCCCCCCCEEEEEEECCCCCCHHCCCCCHHHHCCCCCEECCCCCCCCCCCEEEEEEEE
IQVPDIGDASNVDVIEVLVSVGDMITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGD
EECCCCCCCCCHHHHHHHHHHCCCEECCCCEEEEECCCCEEECCCCCCCCEEEEEEEECC
KVSQGSVIATVETTAVGAAAPAPVAQAPAVQEVAPVAAQAPASRPPVPHHPSAGAPVSTG
CCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
AVHASPAVRRLAREFGVDLTQVTGSGRKGRIMKEDVQAYVKYELSRPKATAATSVGAGNG
CEECCHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHEECCCCCC
GGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFR
CCEEEEEECCCCHHHCCCCHHCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHH
KQQNDAAAKKKADYKITPLVFMMKAVAKTLQQFPVFNSSLSSDGESLIQKKYFHIGVAVD
HHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEEEEEEE
TPNGLVVPVVRDVDKKGIIELSRELADISIRARDGKLKSADMQGSCFTISSLGGIGGTAF
CCCCEEEEHECCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCC
TPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDGAMAARFSVTLSGI
CCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LSDIRTLIL
HHHHHHHHC
>Mature Secondary Structure 
AELKEVFVPDIGGDEVQVIEICAAVGDTLAADESIITVESDKATMDIPAPFAGVLAELK
CCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCEEECCCCHHHHHHHHH
VAVGDKVSEGTLIAMMQAAGAAAADPAPVAAPSSAPAAAPVQAAPAPAVPAATSTQAVET
HHHCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHE
KVVEVAVPDIGGDTDVSVIEVLVAVGDKIEVDSGLITLETDKATMDVPSPFAGVVKEVKV
EEEEEEECCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHH
AVGDKVSEGSLVIMLEVGGAAPAVAASAPTVAAQAAPAATVAPVAPASATPTASVVTVKE
HHCCCCCCCCEEEEEEECCCCCCHHCCCCCHHHHCCCCCEECCCCCCCCCCCEEEEEEEE
IQVPDIGDASNVDVIEVLVSVGDMITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGD
EECCCCCCCCCHHHHHHHHHHCCCEECCCCEEEEECCCCEEECCCCCCCCEEEEEEEECC
KVSQGSVIATVETTAVGAAAPAPVAQAPAVQEVAPVAAQAPASRPPVPHHPSAGAPVSTG
CCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
AVHASPAVRRLAREFGVDLTQVTGSGRKGRIMKEDVQAYVKYELSRPKATAATSVGAGNG
CEECCHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHEECCCCCC
GGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFR
CCEEEEEECCCCHHHCCCCHHCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHH
KQQNDAAAKKKADYKITPLVFMMKAVAKTLQQFPVFNSSLSSDGESLIQKKYFHIGVAVD
HHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEEEEEEE
TPNGLVVPVVRDVDKKGIIELSRELADISIRARDGKLKSADMQGSCFTISSLGGIGGTAF
CCCCEEEEHECCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCC
TPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDGAMAARFSVTLSGI
CCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LSDIRTLIL
HHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]