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The map label for this gene is glpK [H]

Identifier: 120597210

GI number: 120597210

Start: 409599

End: 411083

Strand: Direct

Name: glpK [H]

Synonym: Sputw3181_0379

Alternate gene names: 120597210

Gene position: 409599-411083 (Clockwise)

Preceding gene: 120597209

Following gene: 120597212

Centisome position: 8.7

GC content: 50.03

Gene sequence:

>1485_bases
GTGCAGAAAAAATACGTGGTGGCCTTAGATCAAGGCACGACAAGCTCAAGGGCGATAGTGTTCGATCACGACGCAAACAT
TGTCAGCGTGTCCCAGCGGGAATTTACCCAGTTGTATCCTAATCCAGGTTGGGTCGAGCATGATCCCATGGAGATTTGGG
CAAGCCAAAGTTCAGTGTTAGTTGAAGCGCTGGCGCGTGCGGGTATTCACAGCGATGAAGTGGCGGCCATTGGCATAACC
AATCAGCGGGAAACCACCGTTATTTGGGAGAAGGCCACAGGTAAACCTATTTATAACGCGATTGTGTGGCAGTGCCGTCG
CAGCGCTGAGATTTGTGAGCAACTCAAAGCTCAAGGGTTAGAAGAATATGTGCGTGAGAATACCGGTTTATTACTCGACC
CTTATTTTTCAGGCACTAAGATCAAATGGATTTTAGATAATGTGCCCAATGCCCGTGAGCAAGCCGATCGTGGTGAGTTG
TTGTTCGGCACTATCGATACTTGGCTCGTGTGGAAGTTAACCGAGGGCAAGGTGCATGTCACCGATCCCACCAATGCTGC
CCGCACGTTGCTATTCAATATCCATAGCCTGACTTGGGATAATAAGTTACTCGAAGCCTTAGATATTCCCTTGTCTATGT
TGCCTGACGTTAAACCCTCCTGCAGCGTGTATGGCACCACACGGATTGCCGGTGAAGGCAGTGAAATCCAAGTTGCAGGA
ATGGCGGGCGATCAACAGGCTGCGCTTTTTGGTCAGTTATGTGTCGAGCCCGGCATGGCGAAGAATACTTACGGCACTGG
TTGTTTTTTGTTGATGAATACTGGGACCAAAGCGGTGCGCTCAAACCATGGCTTATTGACTACAGTTGCCGTGGGGCCTA
AGGGTGAAGTGAACTATGCCCTCGAAGGTTCAGTTTTTATGGGCGGCGCGACCATTCAATGGTTGCGGGATGAACTCGGG
CTTATTCGCGATGCCAGTGATACCGAATACTTTGCCTCTAAGGTCGCCGATACTAATGGCGTATATCTGGTACCCGCTTT
TGTGGGACTCGGTGCGCCCTATTGGGATCCCAATGCCCGTGGTGCGCTATTTGGTCTGACACGCGGGGCGAATCGTAATC
ATATTATTCGGGCGGCGTTAGAATCTATCGCTTACCAGAGTAAAGATTTACTCGATGCCATGATCAAAGACAGTGGCGTC
AGCCTTAAGCGTTTAAAAGTGGATGGTGGCGCGGTCGCTAATGATTTTTTGATGCAATTTCAAGCCGACATTACCGATGT
CGAAGTGTTAAGGCCAAGCGTTTGTGAGACAACCGCTTTGGGCGCGGCATTTTTAGCTGGGTTGGCCGTGGGCTTTTGGG
AGAGTGTGATAGAGCTTGAGCACAAAGCCTGCATCGATAAGCATTTTATCCCTAATATCGATGCCCAGACTCGAGTGCAG
CTATATGCAGGTTGGCAGGATGCGGTGGCCAGAACTCGAACATAA

Upstream 100 bases:

>100_bases
TCACGGCGGAAAATTCCGAATATATTCACTGCATAAGTAGTAAGTCATTTCGATTCTCGCTGATATAAATGTCGACAGAG
GATCCTGATAGGATAGTAGC

Downstream 100 bases:

>100_bases
CTCATTGAATTTTAGCATGCTTAGATTTGGTGTATTGTTTGGTTGTTGCTAAGAAAAAGGCGCTAATCCTGTGGATTGCG
CCTTTTTCTTTTTGTGTAAT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MQKKYVVALDQGTTSSRAIVFDHDANIVSVSQREFTQLYPNPGWVEHDPMEIWASQSSVLVEALARAGIHSDEVAAIGIT
NQRETTVIWEKATGKPIYNAIVWQCRRSAEICEQLKAQGLEEYVRENTGLLLDPYFSGTKIKWILDNVPNAREQADRGEL
LFGTIDTWLVWKLTEGKVHVTDPTNAARTLLFNIHSLTWDNKLLEALDIPLSMLPDVKPSCSVYGTTRIAGEGSEIQVAG
MAGDQQAALFGQLCVEPGMAKNTYGTGCFLLMNTGTKAVRSNHGLLTTVAVGPKGEVNYALEGSVFMGGATIQWLRDELG
LIRDASDTEYFASKVADTNGVYLVPAFVGLGAPYWDPNARGALFGLTRGANRNHIIRAALESIAYQSKDLLDAMIKDSGV
SLKRLKVDGGAVANDFLMQFQADITDVEVLRPSVCETTALGAAFLAGLAVGFWESVIELEHKACIDKHFIPNIDAQTRVQ
LYAGWQDAVARTRT

Sequences:

>Translated_494_residues
MQKKYVVALDQGTTSSRAIVFDHDANIVSVSQREFTQLYPNPGWVEHDPMEIWASQSSVLVEALARAGIHSDEVAAIGIT
NQRETTVIWEKATGKPIYNAIVWQCRRSAEICEQLKAQGLEEYVRENTGLLLDPYFSGTKIKWILDNVPNAREQADRGEL
LFGTIDTWLVWKLTEGKVHVTDPTNAARTLLFNIHSLTWDNKLLEALDIPLSMLPDVKPSCSVYGTTRIAGEGSEIQVAG
MAGDQQAALFGQLCVEPGMAKNTYGTGCFLLMNTGTKAVRSNHGLLTTVAVGPKGEVNYALEGSVFMGGATIQWLRDELG
LIRDASDTEYFASKVADTNGVYLVPAFVGLGAPYWDPNARGALFGLTRGANRNHIIRAALESIAYQSKDLLDAMIKDSGV
SLKRLKVDGGAVANDFLMQFQADITDVEVLRPSVCETTALGAAFLAGLAVGFWESVIELEHKACIDKHFIPNIDAQTRVQ
LYAGWQDAVARTRT
>Mature_494_residues
MQKKYVVALDQGTTSSRAIVFDHDANIVSVSQREFTQLYPNPGWVEHDPMEIWASQSSVLVEALARAGIHSDEVAAIGIT
NQRETTVIWEKATGKPIYNAIVWQCRRSAEICEQLKAQGLEEYVRENTGLLLDPYFSGTKIKWILDNVPNAREQADRGEL
LFGTIDTWLVWKLTEGKVHVTDPTNAARTLLFNIHSLTWDNKLLEALDIPLSMLPDVKPSCSVYGTTRIAGEGSEIQVAG
MAGDQQAALFGQLCVEPGMAKNTYGTGCFLLMNTGTKAVRSNHGLLTTVAVGPKGEVNYALEGSVFMGGATIQWLRDELG
LIRDASDTEYFASKVADTNGVYLVPAFVGLGAPYWDPNARGALFGLTRGANRNHIIRAALESIAYQSKDLLDAMIKDSGV
SLKRLKVDGGAVANDFLMQFQADITDVEVLRPSVCETTALGAAFLAGLAVGFWESVIELEHKACIDKHFIPNIDAQTRVQ
LYAGWQDAVARTRT

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=499, Percent_Identity=47.8957915831663, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI42794763, Length=504, Percent_Identity=47.6190476190476, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI4504007, Length=499, Percent_Identity=47.8957915831663, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI41393575, Length=506, Percent_Identity=46.4426877470356, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI88196792, Length=504, Percent_Identity=33.7301587301587, Blast_Score=300, Evalue=2e-81,
Organism=Escherichia coli, GI1790361, Length=491, Percent_Identity=71.8940936863544, Blast_Score=764, Evalue=0.0,
Organism=Escherichia coli, GI1787789, Length=490, Percent_Identity=27.3469387755102, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789987, Length=493, Percent_Identity=24.7464503042596, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1790005, Length=507, Percent_Identity=23.2741617357002, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789138, Length=485, Percent_Identity=23.0927835051546, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17535599, Length=498, Percent_Identity=41.7670682730924, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71986140, Length=521, Percent_Identity=24.7600767754319, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71986147, Length=264, Percent_Identity=30.3030303030303, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321755, Length=486, Percent_Identity=39.7119341563786, Blast_Score=306, Evalue=5e-84,
Organism=Drosophila melanogaster, GI17864214, Length=506, Percent_Identity=43.4782608695652, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655555, Length=500, Percent_Identity=40.8, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655560, Length=500, Percent_Identity=40.8, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655570, Length=500, Percent_Identity=40.8, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655574, Length=500, Percent_Identity=40.8, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655565, Length=500, Percent_Identity=40.8, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24654573, Length=445, Percent_Identity=44.4943820224719, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI19922058, Length=525, Percent_Identity=32.5714285714286, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24652871, Length=492, Percent_Identity=33.5365853658537, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24656367, Length=510, Percent_Identity=28.4313725490196, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI21357867, Length=525, Percent_Identity=27.8095238095238, Blast_Score=199, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24656372, Length=357, Percent_Identity=30.5322128851541, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45552909, Length=302, Percent_Identity=27.4834437086093, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 54043; Mature: 54043

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKYVVALDQGTTSSRAIVFDHDANIVSVSQREFTQLYPNPGWVEHDPMEIWASQSSVL
CCCCEEEEEECCCCCCEEEEEECCCCEEEECHHHHHHHCCCCCCCCCCHHHHHCCCHHHH
VEALARAGIHSDEVAAIGITNQRETTVIWEKATGKPIYNAIVWQCRRSAEICEQLKAQGL
HHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
EEYVRENTGLLLDPYFSGTKIKWILDNVPNAREQADRGELLFGTIDTWLVWKLTEGKVHV
HHHHHCCCCEEEECCCCCCEEEEEECCCCCHHHHCCCCCEEEEEECEEEEEEEECCEEEE
TDPTNAARTLLFNIHSLTWDNKLLEALDIPLSMLPDVKPSCSVYGTTRIAGEGSEIQVAG
ECCCCHHHEEEEEHHHCCCCHHHHHHHCCCHHHCCCCCCCCCEEEEEEEECCCCEEEEEE
MAGDQQAALFGQLCVEPGMAKNTYGTGCFLLMNTGTKAVRSNHGLLTTVAVGPKGEVNYA
CCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHCCCCEEEEEEECCCCCEEEE
LEGSVFMGGATIQWLRDELGLIRDASDTEYFASKVADTNGVYLVPAFVGLGAPYWDPNAR
EECCEEECCCHHHHHHHHHHHEECCCCHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCC
GALFGLTRGANRNHIIRAALESIAYQSKDLLDAMIKDSGVSLKRLKVDGGAVANDFLMQF
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHH
QADITDVEVLRPSVCETTALGAAFLAGLAVGFWESVIELEHKACIDKHFIPNIDAQTRVQ
HCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
LYAGWQDAVARTRT
EEECHHHHHHHCCC
>Mature Secondary Structure
MQKKYVVALDQGTTSSRAIVFDHDANIVSVSQREFTQLYPNPGWVEHDPMEIWASQSSVL
CCCCEEEEEECCCCCCEEEEEECCCCEEEECHHHHHHHCCCCCCCCCCHHHHHCCCHHHH
VEALARAGIHSDEVAAIGITNQRETTVIWEKATGKPIYNAIVWQCRRSAEICEQLKAQGL
HHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
EEYVRENTGLLLDPYFSGTKIKWILDNVPNAREQADRGELLFGTIDTWLVWKLTEGKVHV
HHHHHCCCCEEEECCCCCCEEEEEECCCCCHHHHCCCCCEEEEEECEEEEEEEECCEEEE
TDPTNAARTLLFNIHSLTWDNKLLEALDIPLSMLPDVKPSCSVYGTTRIAGEGSEIQVAG
ECCCCHHHEEEEEHHHCCCCHHHHHHHCCCHHHCCCCCCCCCEEEEEEEECCCCEEEEEE
MAGDQQAALFGQLCVEPGMAKNTYGTGCFLLMNTGTKAVRSNHGLLTTVAVGPKGEVNYA
CCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHCCCCEEEEEEECCCCCEEEE
LEGSVFMGGATIQWLRDELGLIRDASDTEYFASKVADTNGVYLVPAFVGLGAPYWDPNAR
EECCEEECCCHHHHHHHHHHHEECCCCHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCC
GALFGLTRGANRNHIIRAALESIAYQSKDLLDAMIKDSGVSLKRLKVDGGAVANDFLMQF
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHH
QADITDVEVLRPSVCETTALGAAFLAGLAVGFWESVIELEHKACIDKHFIPNIDAQTRVQ
HCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
LYAGWQDAVARTRT
EEECHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA