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The map label for this gene is ydaM [H]

Identifier: 120597069

GI number: 120597069

Start: 261536

End: 262996

Strand: Direct

Name: ydaM [H]

Synonym: Sputw3181_0238

Alternate gene names: 120597069

Gene position: 261536-262996 (Clockwise)

Preceding gene: 120597068

Following gene: 120597070

Centisome position: 5.55

GC content: 40.52

Gene sequence:

>1461_bases
ATGGGCAATAATGTTTGGAATGAGCGAAGGAAGTTTATTTGGCTTTTATCTATATTGTTATTATTTGCTTTTTTAGTGAC
CAGTAGCATCAGTTATAAAGTGGCTCACGATTCGCTCAGCCAACAAATTGAGAGCAATACTTTACCTCTGACGAGTGACA
ATATTTACTCAGAAATTCAGCAGGACTTGTTACGACCGATCTTTATTTCATCTTTGATGGCGCAGGATACCTTTGTTCGT
GATTGGACCTTAAACCATGAACAAGAGCCTGAAAAACTCATTCGTTACCTTAAGGAAATTCAAGAAAAATACGGCACAGT
TACGAGTTTTTTTGTGTCAGAGAAGAGTCGAAATTATTACCATTCCACGGGTATTTTAAAACAAATTCAAGATGTTGAAC
CGGATGATGCTTGGTATTTTCGGGTGCGATCTTTACCTGAATCCGAATATTATGAGATTAATATCGATGCCGATACGGCA
GACCGTAGTAAGACAACTGTATTTGTAAACTATAAAGTTTTTGATTTTGAAGGTAAATTTATTGGGGTGACCGGTGTTGG
TTTAGCAGTTGAGAAAGTCAAATTACTGGTAGAACTTTATCAAAAACGTTATAACCGCCGCGTTTTTTTCACGGATAGAT
TAGGTAATGTCACATTACATGGTGATGCCTATGATGGTTCGGATTCACTCCAGACCAGTACTGGGCTAGAACAATTGGCG
ACACGTATTTTAACGAGTCCGAGTGCGGCATTTAGTTATAAGAAAAATGGAAAAACAGTTTATCTCAATAGTCGTTTAGT
ATCTGAATTTAAATGGTATTTGATTGTTGAGCAGGAAGATGCTCCCCAAGAGCAAGATTTACTTAACACTTTTTGGGGAA
ATCTGGCGCTGAGTTTGATTGTGACTATGGGTATTTTATTTATCTCAAACATGACCTTAGGTCGTTATCAGCGCAAGCTA
GAAATGATGGCGTCTACCGATAAGCTCACTGGCGCCGCTAACCGTCAGGTGTTTGAGGAGTACTTTAGGCAGGCGTTAGA
TAAAGCGAAGCTGACACAATCACCCATTTCAATACTCTTGCTGGATATCGACCACTTTAAAAAAGTGAATGACAGTTATG
GGCATGGAATGGGGGACTTAGTGTTAAAAACCATGGCTAACATGCTACGTGGTTTACTACAGCCGCAGGATATCCTATGT
CGATGGGGTGGTGAGGAGTTTTTAATTTTGCTTCCAGAAATGGACTTAAGTCGGGCTGCAGAATTTGCTGAACAGATCCG
TGACCTTATCTCTCTACGGGAAATCAAAGTGAATGGATTGCACATCTCTATTACTGCCAGTATTGGCGTCGCAGAGCACC
AAGCACAAGAGCCAGCGGAAGATCTTATCAAACGTGCCGATCTGGCCTTGTATCAGGCAAAAGCAGCGGGTCGAAATCAA
GTGGTATTGAATCATCCATGA

Upstream 100 bases:

>100_bases
TGTTGTGCGGTACTTGCACAATTGAGATACATTTATGCAACTGAAAAAGCGTTAAAAAGAATAAGCTACTCAAAGTGTCG
CGCTGCGGTTGGAGTTGAGA

Downstream 100 bases:

>100_bases
TGCTGTTCGGCGCTGCTTTGAGTCATATTGAGTCGTTTAGGGTTATTGCGTTGCTTGATTGTGCTACTGTTATGTCGCGG
TTTACACTCTGACTTTATGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MGNNVWNERRKFIWLLSILLLFAFLVTSSISYKVAHDSLSQQIESNTLPLTSDNIYSEIQQDLLRPIFISSLMAQDTFVR
DWTLNHEQEPEKLIRYLKEIQEKYGTVTSFFVSEKSRNYYHSTGILKQIQDVEPDDAWYFRVRSLPESEYYEINIDADTA
DRSKTTVFVNYKVFDFEGKFIGVTGVGLAVEKVKLLVELYQKRYNRRVFFTDRLGNVTLHGDAYDGSDSLQTSTGLEQLA
TRILTSPSAAFSYKKNGKTVYLNSRLVSEFKWYLIVEQEDAPQEQDLLNTFWGNLALSLIVTMGILFISNMTLGRYQRKL
EMMASTDKLTGAANRQVFEEYFRQALDKAKLTQSPISILLLDIDHFKKVNDSYGHGMGDLVLKTMANMLRGLLQPQDILC
RWGGEEFLILLPEMDLSRAAEFAEQIRDLISLREIKVNGLHISITASIGVAEHQAQEPAEDLIKRADLALYQAKAAGRNQ
VVLNHP

Sequences:

>Translated_486_residues
MGNNVWNERRKFIWLLSILLLFAFLVTSSISYKVAHDSLSQQIESNTLPLTSDNIYSEIQQDLLRPIFISSLMAQDTFVR
DWTLNHEQEPEKLIRYLKEIQEKYGTVTSFFVSEKSRNYYHSTGILKQIQDVEPDDAWYFRVRSLPESEYYEINIDADTA
DRSKTTVFVNYKVFDFEGKFIGVTGVGLAVEKVKLLVELYQKRYNRRVFFTDRLGNVTLHGDAYDGSDSLQTSTGLEQLA
TRILTSPSAAFSYKKNGKTVYLNSRLVSEFKWYLIVEQEDAPQEQDLLNTFWGNLALSLIVTMGILFISNMTLGRYQRKL
EMMASTDKLTGAANRQVFEEYFRQALDKAKLTQSPISILLLDIDHFKKVNDSYGHGMGDLVLKTMANMLRGLLQPQDILC
RWGGEEFLILLPEMDLSRAAEFAEQIRDLISLREIKVNGLHISITASIGVAEHQAQEPAEDLIKRADLALYQAKAAGRNQ
VVLNHP
>Mature_485_residues
GNNVWNERRKFIWLLSILLLFAFLVTSSISYKVAHDSLSQQIESNTLPLTSDNIYSEIQQDLLRPIFISSLMAQDTFVRD
WTLNHEQEPEKLIRYLKEIQEKYGTVTSFFVSEKSRNYYHSTGILKQIQDVEPDDAWYFRVRSLPESEYYEINIDADTAD
RSKTTVFVNYKVFDFEGKFIGVTGVGLAVEKVKLLVELYQKRYNRRVFFTDRLGNVTLHGDAYDGSDSLQTSTGLEQLAT
RILTSPSAAFSYKKNGKTVYLNSRLVSEFKWYLIVEQEDAPQEQDLLNTFWGNLALSLIVTMGILFISNMTLGRYQRKLE
MMASTDKLTGAANRQVFEEYFRQALDKAKLTQSPISILLLDIDHFKKVNDSYGHGMGDLVLKTMANMLRGLLQPQDILCR
WGGEEFLILLPEMDLSRAAEFAEQIRDLISLREIKVNGLHISITASIGVAEHQAQEPAEDLIKRADLALYQAKAAGRNQV
VLNHP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=41.0714285714286, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787262, Length=174, Percent_Identity=38.5057471264368, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI87082007, Length=199, Percent_Identity=36.6834170854271, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=36.7469879518072, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=36.7088607594937, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786584, Length=192, Percent_Identity=31.7708333333333, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1788381, Length=188, Percent_Identity=34.0425531914894, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1787541, Length=176, Percent_Identity=33.5227272727273, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787802, Length=191, Percent_Identity=31.413612565445, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=30.7228915662651, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081977, Length=166, Percent_Identity=31.3253012048193, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87081921, Length=137, Percent_Identity=29.9270072992701, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787056, Length=160, Percent_Identity=30.625, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788956, Length=149, Percent_Identity=32.2147651006711, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87081974, Length=192, Percent_Identity=25, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656 [H]

Pfam domain/function: PF00990 GGDEF; PF08448 PAS_4 [H]

EC number: NA

Molecular weight: Translated: 55640; Mature: 55509

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNNVWNERRKFIWLLSILLLFAFLVTSSISYKVAHDSLSQQIESNTLPLTSDNIYSEIQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHH
QDLLRPIFISSLMAQDTFVRDWTLNHEQEPEKLIRYLKEIQEKYGTVTSFFVSEKSRNYY
HHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEE
HSTGILKQIQDVEPDDAWYFRVRSLPESEYYEINIDADTADRSKTTVFVNYKVFDFEGKF
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCEEEEEEEEEEEECCCE
IGVTGVGLAVEKVKLLVELYQKRYNRRVFFTDRLGNVTLHGDAYDGSDSLQTSTGLEQLA
EEEECCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCCCCCCHHHCCHHHHH
TRILTSPSAAFSYKKNGKTVYLNSRLVSEFKWYLIVEQEDAPQEQDLLNTFWGNLALSLI
HHHHCCCCHHEEECCCCCEEEEEHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
VTMGILFISNMTLGRYQRKLEMMASTDKLTGAANRQVFEEYFRQALDKAKLTQSPISILL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LDIDHFKKVNDSYGHGMGDLVLKTMANMLRGLLQPQDILCRWGGEEFLILLPEMDLSRAA
EEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHEECCCEEEEEECCCCHHHHH
EFAEQIRDLISLREIKVNGLHISITASIGVAEHQAQEPAEDLIKRADLALYQAKAAGRNQ
HHHHHHHHHHHHHHEEECCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
VVLNHP
EEECCC
>Mature Secondary Structure 
GNNVWNERRKFIWLLSILLLFAFLVTSSISYKVAHDSLSQQIESNTLPLTSDNIYSEIQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHH
QDLLRPIFISSLMAQDTFVRDWTLNHEQEPEKLIRYLKEIQEKYGTVTSFFVSEKSRNYY
HHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEE
HSTGILKQIQDVEPDDAWYFRVRSLPESEYYEINIDADTADRSKTTVFVNYKVFDFEGKF
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCEEEEEEEEEEEECCCE
IGVTGVGLAVEKVKLLVELYQKRYNRRVFFTDRLGNVTLHGDAYDGSDSLQTSTGLEQLA
EEEECCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCCCCCCHHHCCHHHHH
TRILTSPSAAFSYKKNGKTVYLNSRLVSEFKWYLIVEQEDAPQEQDLLNTFWGNLALSLI
HHHHCCCCHHEEECCCCCEEEEEHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
VTMGILFISNMTLGRYQRKLEMMASTDKLTGAANRQVFEEYFRQALDKAKLTQSPISILL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LDIDHFKKVNDSYGHGMGDLVLKTMANMLRGLLQPQDILCRWGGEEFLILLPEMDLSRAA
EEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHEECCCEEEEEECCCCHHHHH
EFAEQIRDLISLREIKVNGLHISITASIGVAEHQAQEPAEDLIKRADLALYQAKAAGRNQ
HHHHHHHHHHHHHHEEECCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
VVLNHP
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]