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The map label for this gene is yflS [H]

Identifier: 120597029

GI number: 120597029

Start: 221141

End: 222544

Strand: Direct

Name: yflS [H]

Synonym: Sputw3181_0197

Alternate gene names: 120597029

Gene position: 221141-222544 (Clockwise)

Preceding gene: 120597028

Following gene: 120597030

Centisome position: 4.7

GC content: 45.8

Gene sequence:

>1404_bases
ATGAATAAAAAAATCATCTCATGGCTGATTATATTGGCAGTAGGTGGTGGAATTTGGTTGACTCCCGTACCAGCGGGCGT
TGATCCAACTGCCTGGCACTTGTTAGCCATTTTCGCTGCAACTGTGTTAGGTCTTATCCTAGAGCCTATTCCAATGGGTG
CCGTTGCTATCGGGGGAATAACAATAACGGCATTGACTGGAACATTAACAATTAAAGACTCGCTGACCGGGTTCTCTAAT
GATGTTATTTGGTTAATTGTGACAGCTTTTTTTATCTCAAGAGCGTTTATCAAGAGCGGGCTGGGAGCGCGCATTGGTTT
TGTATTTATGAGATTACTTGGCAAAAAAACACTGAGTCTATCCTATGGCTTGCTGTTAACAGATTTGATCTTAGCGCCAG
CGATTCCATCTAATACTGCTCGGACAGGTGGGATTGTGTTCCCTCTGGCAAACTCAGTCGCAGATGCTTATGGTTCAAAA
GCGGAAGAGGGGACGTCAGGTAAGCTAGGCAGCTTCTTGATGACAACAGTTTTTCATGGCACCACTATCACTGGCGCTAT
GTTTTTAACTGGTATGGCTGCGAATCCACTCGCGGCTAAATTAGCTGCGGATATGGGAGTGGAGATCACTTGGGGTCAAT
GGGCGTTAGCTGCATTAGTGCCAGGAATTATCGCACTGATATTAATGCCATTATTTGCTTACATGCTAAATAAACCGAGC
ATTACATCTACTCCACAAGCCCCTCAGATTGCTGCAAAAGCGCTTGCTGATATGGGCAAAATGAAGCTAACTGAGTGGGT
TACACTGGGGACCTTTATTGCCTTGATTGCTCTGTGGATTGGTGGTCCTGCACTTGGTGTCAGTGGTACAACGGCTGCTA
TGGTTGGTTTAATTATACTTGTCACTGCTAATGTGTTGAGTTGGGATGATGTGCTGAAGGAAAAAGGGGCTTGGAATACG
CTGGTTTGGTTTTCTGCGTTGGTGATGATGGCAACCTTCCTAAACAAACTAGGTTTAATCACATGGTTCGGCACCAATAT
CGCGACTAACCTGAGCGGGTTAGATTGGCCAGTTGCACTGTTTTCGTTGGTATTAATCTACTTTTATATCCATTACGCCT
TTGCATCTGCAACCGCTCATGTTGCTGCACTTTACTCTGTGTTTCTTGCTGTGGCGATACAGCTGGGGGCCCCTGGTCTT
TTAGCTGCGCTTATCTTTGGTTTTACTTCTAATTTATATGCGTGTGGTACTCACTACGGAACAGGCCCTGCACCTATCCT
TTTTGGCGCGGGTCATGTGTCGCTGCCTAAATGGTGGAGTGTGGGTTTTGCCATGGGGCTCTTACAAATTGCCATTTGGA
CCCTTGTGGGCGGAATTTGGTGGAAAGTGATCGGGCTATATTAA

Upstream 100 bases:

>100_bases
GGTTACAGCGTAACTCAAACCTATGTTGTAGCAGTGGAAAACGATGATGTTTCACCATTGTGCAGCAATAACTTCAAATA
AATTTAAATTGGTGCTTACC

Downstream 100 bases:

>100_bases
TCATGTGTCGGGCGGGGTAACTCTTTCCCCCGCTCTAGCTTGCTGTAAATAAATACAAGGGAACGTTATGACAACTATAA
AGAAAGTCGATTTTATTGAC

Product: anion transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MNKKIISWLIILAVGGGIWLTPVPAGVDPTAWHLLAIFAATVLGLILEPIPMGAVAIGGITITALTGTLTIKDSLTGFSN
DVIWLIVTAFFISRAFIKSGLGARIGFVFMRLLGKKTLSLSYGLLLTDLILAPAIPSNTARTGGIVFPLANSVADAYGSK
AEEGTSGKLGSFLMTTVFHGTTITGAMFLTGMAANPLAAKLAADMGVEITWGQWALAALVPGIIALILMPLFAYMLNKPS
ITSTPQAPQIAAKALADMGKMKLTEWVTLGTFIALIALWIGGPALGVSGTTAAMVGLIILVTANVLSWDDVLKEKGAWNT
LVWFSALVMMATFLNKLGLITWFGTNIATNLSGLDWPVALFSLVLIYFYIHYAFASATAHVAALYSVFLAVAIQLGAPGL
LAALIFGFTSNLYACGTHYGTGPAPILFGAGHVSLPKWWSVGFAMGLLQIAIWTLVGGIWWKVIGLY

Sequences:

>Translated_467_residues
MNKKIISWLIILAVGGGIWLTPVPAGVDPTAWHLLAIFAATVLGLILEPIPMGAVAIGGITITALTGTLTIKDSLTGFSN
DVIWLIVTAFFISRAFIKSGLGARIGFVFMRLLGKKTLSLSYGLLLTDLILAPAIPSNTARTGGIVFPLANSVADAYGSK
AEEGTSGKLGSFLMTTVFHGTTITGAMFLTGMAANPLAAKLAADMGVEITWGQWALAALVPGIIALILMPLFAYMLNKPS
ITSTPQAPQIAAKALADMGKMKLTEWVTLGTFIALIALWIGGPALGVSGTTAAMVGLIILVTANVLSWDDVLKEKGAWNT
LVWFSALVMMATFLNKLGLITWFGTNIATNLSGLDWPVALFSLVLIYFYIHYAFASATAHVAALYSVFLAVAIQLGAPGL
LAALIFGFTSNLYACGTHYGTGPAPILFGAGHVSLPKWWSVGFAMGLLQIAIWTLVGGIWWKVIGLY
>Mature_467_residues
MNKKIISWLIILAVGGGIWLTPVPAGVDPTAWHLLAIFAATVLGLILEPIPMGAVAIGGITITALTGTLTIKDSLTGFSN
DVIWLIVTAFFISRAFIKSGLGARIGFVFMRLLGKKTLSLSYGLLLTDLILAPAIPSNTARTGGIVFPLANSVADAYGSK
AEEGTSGKLGSFLMTTVFHGTTITGAMFLTGMAANPLAAKLAADMGVEITWGQWALAALVPGIIALILMPLFAYMLNKPS
ITSTPQAPQIAAKALADMGKMKLTEWVTLGTFIALIALWIGGPALGVSGTTAAMVGLIILVTANVLSWDDVLKEKGAWNT
LVWFSALVMMATFLNKLGLITWFGTNIATNLSGLDWPVALFSLVLIYFYIHYAFASATAHVAALYSVFLAVAIQLGAPGL
LAALIFGFTSNLYACGTHYGTGPAPILFGAGHVSLPKWWSVGFAMGLLQIAIWTLVGGIWWKVIGLY

Specific function: Might be a malate transporter [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family [H]

Homologues:

Organism=Escherichia coli, GI1786986, Length=476, Percent_Identity=36.9747899159664, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1786829, Length=476, Percent_Identity=33.4033613445378, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1789444, Length=479, Percent_Identity=34.446764091858, Blast_Score=255, Evalue=5e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 49181; Mature: 49181

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKIISWLIILAVGGGIWLTPVPAGVDPTAWHLLAIFAATVLGLILEPIPMGAVAIGGI
CCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCE
TITALTGTLTIKDSLTGFSNDVIWLIVTAFFISRAFIKSGLGARIGFVFMRLLGKKTLSL
EEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHH
SYGLLLTDLILAPAIPSNTARTGGIVFPLANSVADAYGSKAEEGTSGKLGSFLMTTVFHG
HHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC
TTITGAMFLTGMAANPLAAKLAADMGVEITWGQWALAALVPGIIALILMPLFAYMLNKPS
CHHHHHHHHHCCCCCHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ITSTPQAPQIAAKALADMGKMKLTEWVTLGTFIALIALWIGGPALGVSGTTAAMVGLIIL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
VTANVLSWDDVLKEKGAWNTLVWFSALVMMATFLNKLGLITWFGTNIATNLSGLDWPVAL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCCCHHHHH
FSLVLIYFYIHYAFASATAHVAALYSVFLAVAIQLGAPGLLAALIFGFTSNLYACGTHYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEECCCCCC
TGPAPILFGAGHVSLPKWWSVGFAMGLLQIAIWTLVGGIWWKVIGLY
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKKIISWLIILAVGGGIWLTPVPAGVDPTAWHLLAIFAATVLGLILEPIPMGAVAIGGI
CCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCE
TITALTGTLTIKDSLTGFSNDVIWLIVTAFFISRAFIKSGLGARIGFVFMRLLGKKTLSL
EEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHH
SYGLLLTDLILAPAIPSNTARTGGIVFPLANSVADAYGSKAEEGTSGKLGSFLMTTVFHG
HHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC
TTITGAMFLTGMAANPLAAKLAADMGVEITWGQWALAALVPGIIALILMPLFAYMLNKPS
CHHHHHHHHHCCCCCHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ITSTPQAPQIAAKALADMGKMKLTEWVTLGTFIALIALWIGGPALGVSGTTAAMVGLIIL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
VTANVLSWDDVLKEKGAWNTLVWFSALVMMATFLNKLGLITWFGTNIATNLSGLDWPVAL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCCCHHHHH
FSLVLIYFYIHYAFASATAHVAALYSVFLAVAIQLGAPGLLAALIFGFTSNLYACGTHYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEECCCCCC
TGPAPILFGAGHVSLPKWWSVGFAMGLLQIAIWTLVGGIWWKVIGLY
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 12949159 [H]