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The map label for this gene is rpoA [H]

Identifier: 120597014

GI number: 120597014

Start: 202545

End: 203534

Strand: Direct

Name: rpoA [H]

Synonym: Sputw3181_0181

Alternate gene names: 120597014

Gene position: 202545-203534 (Clockwise)

Preceding gene: 120597013

Following gene: 120597015

Centisome position: 4.3

GC content: 48.18

Gene sequence:

>990_bases
ATGCAGGGTTCTGTTACAGAATTTCTTAAACCGCGTCTCGTTGATATCGAGCAGGTTAACTCAACACGTGCCAAGGTTAC
ATTGGAACCACTTGAGCGTGGTTTCGGCCACACTTTAGGTAACGCGTTGCGTCGCATCCTATTGTCGTCTATGCCCGGCT
GCGCGGTTACCGAAGTCGAGATTGACGGTGTACTGCACGAATACAGCAGTAAGGAAGGCGTACAAGAAGATATCCTTGAG
ATCTTGTTAAACCTGAAAGGGTTAGCAGTGACTATCGAGGGTAAAGACGAGGCTATGCTTACGTTGAGCAAGTCCGGCGC
AGGCCCTGTCATCGCAGCAGATATCACGCATGATGGTGATGTCACTATCGTGAATCCTGATCATGTTATCTGTCATTTGA
CGGGTAACAATGATATCAGCATGCGTATTCGCGTTGAGCGTGGTCGTGGTTATGTGCCAGCATCTGCTCGTGCACAGACC
GAAGACGATGATCGCCCAATCGGCCGTTTGCTGGTTGATGCTTCTTTCTCGCCAGTTGCACGTATTGCCTACAATGTAGA
AGCAGCTCGTGTTGAACAGCGTACTGACTTGGATAAACTTGTTATCGATATGACCACTAATGGTACTATCGATCCTGAGG
AAGCTATCCGTCGTTCCGCAACTATTTTAGCTGAACAGCTAGATGCGTTTGTTGAGCTGCGTGACGTGACTGAGCCAGAA
TTGAAAGAAGAGAAGCCGGAGTTCGATCCGATTCTGTTGCGTCCTGTCGACGATTTAGAGCTAACTGTACGTTCGGCTAA
CTGCTTGAAAGCCGAAGCGATTCATTACATCGGAGATCTGGTACAGCGTACTGAAGTTGAGCTGCTGAAGACCCCTAACT
TAGGTAAGAAATCTCTTACAGAAATTAAGGACGTTTTGGCTTCTCGCGGACTGTCGTTAGGTATGCGTTTGGAAAACTGG
CCTCCAGCCAGTTTAGCAGACGACCTATAA

Upstream 100 bases:

>100_bases
CAAGCGCGTTCCAGAACGTAGCGATTTGTCTGCGGAAATTAACGAACAGCTGATCGTCGAGCTTTACTCTAAGTAAAGCT
AACAAACAAGAGAGGACACA

Downstream 100 bases:

>100_bases
GTCTCAGGTTTGTACAGATTTAGGTTATAAGGATTAGGTCATGCGCCATCGTAAGAGTGGTCGTCAACTTAACCGCAACA
GCAGCCACCGCCAAGCTATG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MQGSVTEFLKPRLVDIEQVNSTRAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILE
ILLNLKGLAVTIEGKDEAMLTLSKSGAGPVIAADITHDGDVTIVNPDHVICHLTGNNDISMRIRVERGRGYVPASARAQT
EDDDRPIGRLLVDASFSPVARIAYNVEAARVEQRTDLDKLVIDMTTNGTIDPEEAIRRSATILAEQLDAFVELRDVTEPE
LKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENW
PPASLADDL

Sequences:

>Translated_329_residues
MQGSVTEFLKPRLVDIEQVNSTRAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILE
ILLNLKGLAVTIEGKDEAMLTLSKSGAGPVIAADITHDGDVTIVNPDHVICHLTGNNDISMRIRVERGRGYVPASARAQT
EDDDRPIGRLLVDASFSPVARIAYNVEAARVEQRTDLDKLVIDMTTNGTIDPEEAIRRSATILAEQLDAFVELRDVTEPE
LKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENW
PPASLADDL
>Mature_329_residues
MQGSVTEFLKPRLVDIEQVNSTRAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILE
ILLNLKGLAVTIEGKDEAMLTLSKSGAGPVIAADITHDGDVTIVNPDHVICHLTGNNDISMRIRVERGRGYVPASARAQT
EDDDRPIGRLLVDASFSPVARIAYNVEAARVEQRTDLDKLVIDMTTNGTIDPEEAIRRSATILAEQLDAFVELRDVTEPE
LKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENW
PPASLADDL

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=329, Percent_Identity=88.1458966565349, Blast_Score=589, Evalue=1e-169,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 36143; Mature: 36143

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGSVTEFLKPRLVDIEQVNSTRAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE
CCCCHHHHHCCCCCCHHHCCCCCEEEEHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEE
IDGVLHEYSSKEGVQEDILEILLNLKGLAVTIEGKDEAMLTLSKSGAGPVIAADITHDGD
HHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCEEEEEECCCCC
VTIVNPDHVICHLTGNNDISMRIRVERGRGYVPASARAQTEDDDRPIGRLLVDASFSPVA
EEEECCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHEEEECCCCCHH
RIAYNVEAARVEQRTDLDKLVIDMTTNGTIDPEEAIRRSATILAEQLDAFVELRDVTEPE
HHHCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLT
HHHCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
EIKDVLASRGLSLGMRLENWPPASLADDL
HHHHHHHHCCCEECCEECCCCCCCCCCCC
>Mature Secondary Structure
MQGSVTEFLKPRLVDIEQVNSTRAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE
CCCCHHHHHCCCCCCHHHCCCCCEEEEHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEE
IDGVLHEYSSKEGVQEDILEILLNLKGLAVTIEGKDEAMLTLSKSGAGPVIAADITHDGD
HHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCEEEEEECCCCC
VTIVNPDHVICHLTGNNDISMRIRVERGRGYVPASARAQTEDDDRPIGRLLVDASFSPVA
EEEECCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHEEEECCCCCHH
RIAYNVEAARVEQRTDLDKLVIDMTTNGTIDPEEAIRRSATILAEQLDAFVELRDVTEPE
HHHCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLT
HHHCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
EIKDVLASRGLSLGMRLENWPPASLADDL
HHHHHHHHCCCEECCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA