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The map label for this gene is atoC [H]

Identifier: 120596908

GI number: 120596908

Start: 91602

End: 93032

Strand: Direct

Name: atoC [H]

Synonym: Sputw3181_0075

Alternate gene names: 120596908

Gene position: 91602-93032 (Clockwise)

Preceding gene: 120596899

Following gene: 120596909

Centisome position: 1.95

GC content: 46.96

Gene sequence:

>1431_bases
GTGCTAGAATTGGTCGCAAATGAGTCAAAATGTCCAGAAGCAGACAATATGAGCCAGATAGATAATAACCTCAAGCCTCT
TCCATCGGCAGTATCGGTACTGATTGTTGATGATGAACCCGGAATGCGAAGTTTTTTAAATAAAGCCCTTTCGAAGAAGT
TCGCCTTAGTCGAAACCGCTGGCAGTATTGAGGATGCGGAGCAATTACGTAGCCGCTGCCATTTCGACTTGCTGATTGTC
GATATTCGTTTGCCGGGGCGCTCTGGTATTGAATGGCATGAGGCATTAGATGAGCAGGGGCGTCGCTCGGATATTATCTT
TATGACGGGCTATGCCGATATGGAAGTGGCAATCACTGCGCTGCGGGCGGGTGCTTCCGATTTTATTATGAAGCCTTTTC
ATCTTGATCAGATGATGGCGGCTGTAGATCGTTGCATTGAGCGTCGTTTACTCAAGCGCGAGAATCTGATGCTGCGCCGT
GAGGTATCTATTGGGTATTCATCGACCATTATTGGCAGTAGCGAGGCCATGGAGTCAGTAAAACATGTGATAGAACGTGT
GGCTCCAACTAATGCCGTTGTGCTTATACAAGGTGAATCAGGTACGGGTAAAGAACTCGTCGCAAGACAACTGCATTTAT
TGAGTGGCCGACAAGGGCCATTTGTACCCGTTAACTGCGGCTCTATAGCCCCTGAGTTGCTCGAGAGTGAACTCTTTGGC
CATGTTGCGGGTGCATTTACGGGGGCGAAGGGAAATCGAGAGGGGTTATTTAGTTTCGCCTCTGGTGGGACGATTTTTCT
GGATGAAATAGGTGAAATGCCACTGAAAATGCAGACAGCGTTGCTTAGAGTGTTAGAGCAAAAAGCGATCCGACCTGTCG
GCAGTGAAAAAGAAGTGAATATTGATGTTCGGGTTATTGCTGCAACCAACCGAACCCTCTTGGAAGAGGTTGATGCTGGG
AACTTCCGTCGAGATTTGTATTATCGACTCAATGTGCTCGACATCTTAATTCCGCCGTTAAGGGATCGCCCTGAGGATAT
CGTTGAGCTAACCCATCACTTTACTCGTCAGCTTGCCGCTGAGCTTGGTGTCAGGGAAGTAGTGTGGAGCCATGAGGATA
TGGTTAAGCTACAACAGCATGAATGGCCGGGTAATATCCGTGAGCTGCGTAATATGATCGAGCGTTGTATTTTACTTGGT
AAACCCCCCGCGGAATATTGGAAACAACAGGTAAAGAGTGAATCTTCAGCTTCCCAAGGTTATCCCTTAGATTGGCCATT
AAAGGATGTCGAAAAACATCATGTCACCAGCGTGGTAGATTTACATTCTGGTAATAAATCTGCGGCAGCGAGAGACTTAG
GTGTTTCTCGTAAAACCTTAGATCGTAAATATAAAGAGTGGTTTGGCTTAAGCGACGAACAGGAGTCTTAG

Upstream 100 bases:

>100_bases
TAATTTGTGAGCTTGATCCCAAGCTTAACAAATACTCTACAGACAAATTGTCTCAGGACAAACTTCTGATGGTTCATAGT
GACCTATTCATCTATTTTCA

Downstream 100 bases:

>100_bases
GGTGTCTTTCTTTACTCACTTTTTTGGTGTCAGTTGGCAGCAAATGCAGGCCAAGGTGCGTTATCGCATATTAATCCTTA
CCTTATTGCCTATTTTACTG

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MLELVANESKCPEADNMSQIDNNLKPLPSAVSVLIVDDEPGMRSFLNKALSKKFALVETAGSIEDAEQLRSRCHFDLLIV
DIRLPGRSGIEWHEALDEQGRRSDIIFMTGYADMEVAITALRAGASDFIMKPFHLDQMMAAVDRCIERRLLKRENLMLRR
EVSIGYSSTIIGSSEAMESVKHVIERVAPTNAVVLIQGESGTGKELVARQLHLLSGRQGPFVPVNCGSIAPELLESELFG
HVAGAFTGAKGNREGLFSFASGGTIFLDEIGEMPLKMQTALLRVLEQKAIRPVGSEKEVNIDVRVIAATNRTLLEEVDAG
NFRRDLYYRLNVLDILIPPLRDRPEDIVELTHHFTRQLAAELGVREVVWSHEDMVKLQQHEWPGNIRELRNMIERCILLG
KPPAEYWKQQVKSESSASQGYPLDWPLKDVEKHHVTSVVDLHSGNKSAAARDLGVSRKTLDRKYKEWFGLSDEQES

Sequences:

>Translated_476_residues
MLELVANESKCPEADNMSQIDNNLKPLPSAVSVLIVDDEPGMRSFLNKALSKKFALVETAGSIEDAEQLRSRCHFDLLIV
DIRLPGRSGIEWHEALDEQGRRSDIIFMTGYADMEVAITALRAGASDFIMKPFHLDQMMAAVDRCIERRLLKRENLMLRR
EVSIGYSSTIIGSSEAMESVKHVIERVAPTNAVVLIQGESGTGKELVARQLHLLSGRQGPFVPVNCGSIAPELLESELFG
HVAGAFTGAKGNREGLFSFASGGTIFLDEIGEMPLKMQTALLRVLEQKAIRPVGSEKEVNIDVRVIAATNRTLLEEVDAG
NFRRDLYYRLNVLDILIPPLRDRPEDIVELTHHFTRQLAAELGVREVVWSHEDMVKLQQHEWPGNIRELRNMIERCILLG
KPPAEYWKQQVKSESSASQGYPLDWPLKDVEKHHVTSVVDLHSGNKSAAARDLGVSRKTLDRKYKEWFGLSDEQES
>Mature_476_residues
MLELVANESKCPEADNMSQIDNNLKPLPSAVSVLIVDDEPGMRSFLNKALSKKFALVETAGSIEDAEQLRSRCHFDLLIV
DIRLPGRSGIEWHEALDEQGRRSDIIFMTGYADMEVAITALRAGASDFIMKPFHLDQMMAAVDRCIERRLLKRENLMLRR
EVSIGYSSTIIGSSEAMESVKHVIERVAPTNAVVLIQGESGTGKELVARQLHLLSGRQGPFVPVNCGSIAPELLESELFG
HVAGAFTGAKGNREGLFSFASGGTIFLDEIGEMPLKMQTALLRVLEQKAIRPVGSEKEVNIDVRVIAATNRTLLEEVDAG
NFRRDLYYRLNVLDILIPPLRDRPEDIVELTHHFTRQLAAELGVREVVWSHEDMVKLQQHEWPGNIRELRNMIERCILLG
KPPAEYWKQQVKSESSASQGYPLDWPLKDVEKHHVTSVVDLHSGNKSAAARDLGVSRKTLDRKYKEWFGLSDEQES

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=449, Percent_Identity=39.6436525612472, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1790437, Length=439, Percent_Identity=40.3189066059225, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1790299, Length=467, Percent_Identity=38.3297644539615, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1788905, Length=429, Percent_Identity=36.1305361305361, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI1786524, Length=321, Percent_Identity=44.5482866043614, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1789233, Length=317, Percent_Identity=40.3785488958991, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082117, Length=254, Percent_Identity=47.6377952755905, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1789087, Length=233, Percent_Identity=51.931330472103, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=48.0851063829787, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1787583, Length=317, Percent_Identity=39.4321766561514, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87081872, Length=234, Percent_Identity=41.8803418803419, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789828, Length=223, Percent_Identity=40.8071748878924, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI87081858, Length=294, Percent_Identity=30.952380952381, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=30.7692307692308, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53326; Mature: 53326

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELVANESKCPEADNMSQIDNNLKPLPSAVSVLIVDDEPGMRSFLNKALSKKFALVETA
CCEEECCCCCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
GSIEDAEQLRSRCHFDLLIVDIRLPGRSGIEWHEALDEQGRRSDIIFMTGYADMEVAITA
CCHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHH
LRAGASDFIMKPFHLDQMMAAVDRCIERRLLKRENLMLRREVSIGYSSTIIGSSEAMESV
HHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEECCCHHHHHHH
KHVIERVAPTNAVVLIQGESGTGKELVARQLHLLSGRQGPFVPVNCGSIAPELLESELFG
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHH
HVAGAFTGAKGNREGLFSFASGGTIFLDEIGEMPLKMQTALLRVLEQKAIRPVGSEKEVN
HHHHHHCCCCCCCCCCEEECCCCEEEEEHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEE
IDVRVIAATNRTLLEEVDAGNFRRDLYYRLNVLDILIPPLRDRPEDIVELTHHFTRQLAA
EEEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
ELGVREVVWSHEDMVKLQQHEWPGNIRELRNMIERCILLGKPPAEYWKQQVKSESSASQG
HHCHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
YPLDWPLKDVEKHHVTSVVDLHSGNKSAAARDLGVSRKTLDRKYKEWFGLSDEQES
CCCCCCHHHHHHHHHHHEEEECCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLELVANESKCPEADNMSQIDNNLKPLPSAVSVLIVDDEPGMRSFLNKALSKKFALVETA
CCEEECCCCCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
GSIEDAEQLRSRCHFDLLIVDIRLPGRSGIEWHEALDEQGRRSDIIFMTGYADMEVAITA
CCHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHH
LRAGASDFIMKPFHLDQMMAAVDRCIERRLLKRENLMLRREVSIGYSSTIIGSSEAMESV
HHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEECCCHHHHHHH
KHVIERVAPTNAVVLIQGESGTGKELVARQLHLLSGRQGPFVPVNCGSIAPELLESELFG
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHH
HVAGAFTGAKGNREGLFSFASGGTIFLDEIGEMPLKMQTALLRVLEQKAIRPVGSEKEVN
HHHHHHCCCCCCCCCCEEECCCCEEEEEHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEE
IDVRVIAATNRTLLEEVDAGNFRRDLYYRLNVLDILIPPLRDRPEDIVELTHHFTRQLAA
EEEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
ELGVREVVWSHEDMVKLQQHEWPGNIRELRNMIERCILLGKPPAEYWKQQVKSESSASQG
HHCHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
YPLDWPLKDVEKHHVTSVVDLHSGNKSAAARDLGVSRKTLDRKYKEWFGLSDEQES
CCCCCCHHHHHHHHHHHEEEECCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]