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The map label for this gene is hemF [H]

Identifier: 120596863

GI number: 120596863

Start: 34828

End: 35736

Strand: Direct

Name: hemF [H]

Synonym: Sputw3181_0030

Alternate gene names: 120596863

Gene position: 34828-35736 (Clockwise)

Preceding gene: 120596862

Following gene: 120596865

Centisome position: 0.74

GC content: 50.61

Gene sequence:

>909_bases
ATGAGTGTGCCTGATGTTGCAGTGGTAAAAGCGTTTTTAATCGATCTACAAAACCGAATTTGTGCCGGTTTACAGGCGCT
GGATGGCCAAGCCACGTTCGCCGCCGATTCATGGACTCGCGCCGAAGGCGGTGGTGGTACGAGCCGAGTATTAACTCAAG
GTGCAGTGTTCGAGCAAGCTGGGGTTAACTTTTCCCATGTGACTGGCGCTGCTATGCCTGCGTCGGCTACGGCGCATCGC
CCAGAATTGGCGGGGCGTAGTTTTGAAGCTATGGGCGTTTCTTTAGTTATTCATCCTAATAATCCTTATATCCCAACGAC
TCATGCCAATGTGCGTTTCTTTATTGCTCAAAAAGAGGGCGCCGATCCCGTGTGGTGGTTTGGCGGTGGCTTTGATTTAA
CGCCTTATTATCCTTTCGAGGAAGATGTGCGCGAGTGGCACCAGACTTCTAAGGATATTTGCGCGCCTTTCGGGGATGAG
GTTTATCCCAAGTATAAGAAATGGTGCGATGAGTATTTCTTCCTGCCGCACCGTAATGAAACTCGCGGCGTGGGTGGATT
GTTTTTCGATGACTTGAATCAAGACGGATTCGAGCAAAGCTTTAGCTTTATGCAAGCTGTAGGTAATGGCTTCTTAACGG
CCTATGCGCCGATTGTCGAGCGTCGTAAAGAGACTGAATTCGGTGAGCGTGAGCGCCAGTTCCAACTCTACCGTCGTGGT
CGTTATGTTGAGTTCAACTTAGTCTACGACAGGGGCACTTTGTTTGGCCTGCAGACAGGCGGCCGTACCGAGTCTATCTT
AATGTCGATGCCGCCATTAGTGCGTTGGCAATATGCTTATACACCAGAGGCGGGAAGCCCAGAGGCAGATTTGTACGATA
ACTACCTCAAACCCCGCGACTGGGTTTAG

Upstream 100 bases:

>100_bases
GAACTTGGTGAGCAACGTCAGCCCTCGACCATTATCGATGCGCGAAGCGGTAAGATTTTACGAAATGGACAATGAGCAAA
CCAATAATAAGGATAACAAG

Downstream 100 bases:

>100_bases
CCAGTAATCTTATGAAGTGGATACAACATCCACTTTATTGATTACGCATATGATCAGCCAGAGTTGAAATCGCCTCAAAG
ATGGCGGCTCGATGCTCTGG

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSVPDVAVVKAFLIDLQNRICAGLQALDGQATFAADSWTRAEGGGGTSRVLTQGAVFEQAGVNFSHVTGAAMPASATAHR
PELAGRSFEAMGVSLVIHPNNPYIPTTHANVRFFIAQKEGADPVWWFGGGFDLTPYYPFEEDVREWHQTSKDICAPFGDE
VYPKYKKWCDEYFFLPHRNETRGVGGLFFDDLNQDGFEQSFSFMQAVGNGFLTAYAPIVERRKETEFGERERQFQLYRRG
RYVEFNLVYDRGTLFGLQTGGRTESILMSMPPLVRWQYAYTPEAGSPEADLYDNYLKPRDWV

Sequences:

>Translated_302_residues
MSVPDVAVVKAFLIDLQNRICAGLQALDGQATFAADSWTRAEGGGGTSRVLTQGAVFEQAGVNFSHVTGAAMPASATAHR
PELAGRSFEAMGVSLVIHPNNPYIPTTHANVRFFIAQKEGADPVWWFGGGFDLTPYYPFEEDVREWHQTSKDICAPFGDE
VYPKYKKWCDEYFFLPHRNETRGVGGLFFDDLNQDGFEQSFSFMQAVGNGFLTAYAPIVERRKETEFGERERQFQLYRRG
RYVEFNLVYDRGTLFGLQTGGRTESILMSMPPLVRWQYAYTPEAGSPEADLYDNYLKPRDWV
>Mature_301_residues
SVPDVAVVKAFLIDLQNRICAGLQALDGQATFAADSWTRAEGGGGTSRVLTQGAVFEQAGVNFSHVTGAAMPASATAHRP
ELAGRSFEAMGVSLVIHPNNPYIPTTHANVRFFIAQKEGADPVWWFGGGFDLTPYYPFEEDVREWHQTSKDICAPFGDEV
YPKYKKWCDEYFFLPHRNETRGVGGLFFDDLNQDGFEQSFSFMQAVGNGFLTAYAPIVERRKETEFGERERQFQLYRRGR
YVEFNLVYDRGTLFGLQTGGRTESILMSMPPLVRWQYAYTPEAGSPEADLYDNYLKPRDWV

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III [H]

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI41393599, Length=302, Percent_Identity=45.6953642384106, Blast_Score=285, Evalue=5e-77,
Organism=Escherichia coli, GI1788777, Length=299, Percent_Identity=73.9130434782609, Blast_Score=466, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6320249, Length=320, Percent_Identity=45, Blast_Score=274, Evalue=2e-74,
Organism=Drosophila melanogaster, GI17864388, Length=303, Percent_Identity=47.5247524752475, Blast_Score=290, Evalue=7e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001260
- InterPro:   IPR018375 [H]

Pfam domain/function: PF01218 Coprogen_oxidas [H]

EC number: =1.3.3.3 [H]

Molecular weight: Translated: 34085; Mature: 33954

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPDVAVVKAFLIDLQNRICAGLQALDGQATFAADSWTRAEGGGGTSRVLTQGAVFEQA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHCCCHHHHH
GVNFSHVTGAAMPASATAHRPELAGRSFEAMGVSLVIHPNNPYIPTTHANVRFFIAQKEG
CCCHHHHCCCCCCCCCCCCCCHHCCCCEECCCEEEEEECCCCCCCCCCCCEEEEEEECCC
ADPVWWFGGGFDLTPYYPFEEDVREWHQTSKDICAPFGDEVYPKYKKWCDEYFFLPHRNE
CCEEEEECCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHEECCCCCC
TRGVGGLFFDDLNQDGFEQSFSFMQAVGNGFLTAYAPIVERRKETEFGERERQFQLYRRG
CCCCCCHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
RYVEFNLVYDRGTLFGLQTGGRTESILMSMPPLVRWQYAYTPEAGSPEADLYDNYLKPRD
CEEEEEEEEECCEEEEECCCCCCHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCC
WV
CC
>Mature Secondary Structure 
SVPDVAVVKAFLIDLQNRICAGLQALDGQATFAADSWTRAEGGGGTSRVLTQGAVFEQA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHCCCHHHHH
GVNFSHVTGAAMPASATAHRPELAGRSFEAMGVSLVIHPNNPYIPTTHANVRFFIAQKEG
CCCHHHHCCCCCCCCCCCCCCHHCCCCEECCCEEEEEECCCCCCCCCCCCEEEEEEECCC
ADPVWWFGGGFDLTPYYPFEEDVREWHQTSKDICAPFGDEVYPKYKKWCDEYFFLPHRNE
CCEEEEECCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHEECCCCCC
TRGVGGLFFDDLNQDGFEQSFSFMQAVGNGFLTAYAPIVERRKETEFGERERQFQLYRRG
CCCCCCHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
RYVEFNLVYDRGTLFGLQTGGRTESILMSMPPLVRWQYAYTPEAGSPEADLYDNYLKPRD
CEEEEEEEEECCEEEEECCCCCCHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCC
WV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA