| Definition | Psychromonas ingrahamii 37, complete genome. |
|---|---|
| Accession | NC_008709 |
| Length | 4,559,598 |
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The map label for this gene is dnaN [H]
Identifier: 119947309
GI number: 119947309
Start: 4525767
End: 4526870
Strand: Direct
Name: dnaN [H]
Synonym: Ping_3715
Alternate gene names: 119947309
Gene position: 4525767-4526870 (Clockwise)
Preceding gene: 119947308
Following gene: 119947310
Centisome position: 99.26
GC content: 40.49
Gene sequence:
>1104_bases ATGCATTTTTCAATTTCTAGAGAAGCATTTCTTCGTCCGCTGCAACTCGTTGTTAGCTCCGTCGCCGGCGGGCGTCCCGC GTTACCTATAATGGCTAATATTTTAATTGAAGTTAAAGATAACCAATTAAGCCTAACGGGAACAGATCTGGAAGTTGAAC TGATTGCCGTTATCGCACTGGAAAATCCCAGTGAGAATGGTGTTATTACTGTTCCTGCTAGAAAATTACTGGATATTTGT CGGGGTTTATCTGAGCCCTGTGAAATCAAGTTTCAGGTAAAAGAAGAGAAAGTAACCATACGCTCAGGCCGTAGTCGTTA CTTACTATCTTCATTGCCAGCTGTTGATTTTCCTAATATAGAATCCTGGCAAAGCCTGGTTGAGTTAACGGTTCCTCAAG CTGAGTTTAAAAAACTGATCGAATTCACCCAGTTTGCGATGGCCAGCCAAGATGTGCGCTATTATCTTAACGGCATGTAT TTTGAAGCTTCTGAACAGACCTTACGCAGCGTAGCAACCGATGGGCATCGACTTGCCTCCTGCTCAATAAAGCTTAATCA AGTTATTGAGCAACAGTCTGTGATTGTGCCAAGAAAAGGGGTGATAGAGTTAGTTAAGCTGCTCGATAATGATCAGGCAA ATGTCACCATTCAAATCGGGCAAAATAATTTGCGCGCCGTAATAGATGGCTTTATTTTTACCACTAAATTGGTCGATGGC CGCTTTCCGGATTATCGCCGCGTTATCCCTAAAAATGGTGATAAAGAGTTAATTTCTAATCGGGAATCATTAAAGCAGGC TTTTTCTCGTGCGGCCATTTTATCCAACGAAAAATTCAGAGGGGTACGTTTAATTCTTTCTGAAAATCTTCTGCAAATTA CCGCAAACAATCCGGAAAGAGAGGAAGCTGAAGAATATATAGATGTTGATTATCACTCCGCCAACTTAGAAATCGGCTTT AATGTAAGCTACGTTTTAGATGTCTTAAACACCCTAAAAAGTGAACAGGTTAAAATAACATTTAGTAATTCAGATCACAG TGCACTAATAGAATCAATTGATAGTGATGACTCACTTTATGTGCTTATGCCAATGCGCTTATAG
Upstream 100 bases:
>100_bases ATTTAAAGGATAAATGGCTTAATGAGTAACTCCTGTCAATTTCCTCCGCGTCTAACATTGACCACTTATTTAATGTAATT GCTATAAGGTAAGTTTGATT
Downstream 100 bases:
>100_bases CAGTTACCTCAGGTGACACAAATCCTCAAAAAAATATTTTCCATTAAAGTGTGCTTATATGTCACTCAGTAGATTGATTA TCCATCAGTTTCGCAATATA
Product: DNA polymerase III, beta subunit
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 367; Mature: 367
Protein sequence:
>367_residues MHFSISREAFLRPLQLVVSSVAGGRPALPIMANILIEVKDNQLSLTGTDLEVELIAVIALENPSENGVITVPARKLLDIC RGLSEPCEIKFQVKEEKVTIRSGRSRYLLSSLPAVDFPNIESWQSLVELTVPQAEFKKLIEFTQFAMASQDVRYYLNGMY FEASEQTLRSVATDGHRLASCSIKLNQVIEQQSVIVPRKGVIELVKLLDNDQANVTIQIGQNNLRAVIDGFIFTTKLVDG RFPDYRRVIPKNGDKELISNRESLKQAFSRAAILSNEKFRGVRLILSENLLQITANNPEREEAEEYIDVDYHSANLEIGF NVSYVLDVLNTLKSEQVKITFSNSDHSALIESIDSDDSLYVLMPMRL
Sequences:
>Translated_367_residues MHFSISREAFLRPLQLVVSSVAGGRPALPIMANILIEVKDNQLSLTGTDLEVELIAVIALENPSENGVITVPARKLLDIC RGLSEPCEIKFQVKEEKVTIRSGRSRYLLSSLPAVDFPNIESWQSLVELTVPQAEFKKLIEFTQFAMASQDVRYYLNGMY FEASEQTLRSVATDGHRLASCSIKLNQVIEQQSVIVPRKGVIELVKLLDNDQANVTIQIGQNNLRAVIDGFIFTTKLVDG RFPDYRRVIPKNGDKELISNRESLKQAFSRAAILSNEKFRGVRLILSENLLQITANNPEREEAEEYIDVDYHSANLEIGF NVSYVLDVLNTLKSEQVKITFSNSDHSALIESIDSDDSLYVLMPMRL >Mature_367_residues MHFSISREAFLRPLQLVVSSVAGGRPALPIMANILIEVKDNQLSLTGTDLEVELIAVIALENPSENGVITVPARKLLDIC RGLSEPCEIKFQVKEEKVTIRSGRSRYLLSSLPAVDFPNIESWQSLVELTVPQAEFKKLIEFTQFAMASQDVRYYLNGMY FEASEQTLRSVATDGHRLASCSIKLNQVIEQQSVIVPRKGVIELVKLLDNDQANVTIQIGQNNLRAVIDGFIFTTKLVDG RFPDYRRVIPKNGDKELISNRESLKQAFSRAAILSNEKFRGVRLILSENLLQITANNPEREEAEEYIDVDYHSANLEIGF NVSYVLDVLNTLKSEQVKITFSNSDHSALIESIDSDDSLYVLMPMRL
Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped
COG id: COG0592
COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: NA
Homologues:
Organism=Escherichia coli, GI1790136, Length=367, Percent_Identity=61.8528610354223, Blast_Score=469, Evalue=1e-133,
Paralogues:
None
Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001001 - InterPro: IPR022635 - InterPro: IPR022637 - InterPro: IPR022634 [H]
Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 41249; Mature: 41249
Theoretical pI: Translated: 4.88; Mature: 4.88
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 1.6 %Met (Translated Protein) 2.5 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 1.6 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MHFSISREAFLRPLQLVVSSVAGGRPALPIMANILIEVKDNQLSLTGTDLEVELIAVIAL CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEEECCEEEEECCCEEEEEEEEEEE ENPSENGVITVPARKLLDICRGLSEPCEIKFQVKEEKVTIRSGRSRYLLSSLPAVDFPNI CCCCCCCEEEECHHHHHHHHHCCCCCCEEEEEEEEEEEEEECCCCHHHHHHCCCCCCCCH ESWQSLVELTVPQAEFKKLIEFTQFAMASQDVRYYLNGMYFEASEQTLRSVATDGHRLAS HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHCCCCEEEE CSIKLNQVIEQQSVIVPRKGVIELVKLLDNDQANVTIQIGQNNLRAVIDGFIFTTKLVDG EEEHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHEEHHHCC RFPDYRRVIPKNGDKELISNRESLKQAFSRAAILSNEKFRGVRLILSENLLQITANNPER CCCCHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCH EEAEEYIDVDYHSANLEIGFNVSYVLDVLNTLKSEQVKITFSNSDHSALIESIDSDDSLY HHHHHHCCCEEECCCEEECCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCEE VLMPMRL EEEECCC >Mature Secondary Structure MHFSISREAFLRPLQLVVSSVAGGRPALPIMANILIEVKDNQLSLTGTDLEVELIAVIAL CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEEECCEEEEECCCEEEEEEEEEEE ENPSENGVITVPARKLLDICRGLSEPCEIKFQVKEEKVTIRSGRSRYLLSSLPAVDFPNI CCCCCCCEEEECHHHHHHHHHCCCCCCEEEEEEEEEEEEEECCCCHHHHHHCCCCCCCCH ESWQSLVELTVPQAEFKKLIEFTQFAMASQDVRYYLNGMYFEASEQTLRSVATDGHRLAS HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHCCCCEEEE CSIKLNQVIEQQSVIVPRKGVIELVKLLDNDQANVTIQIGQNNLRAVIDGFIFTTKLVDG EEEHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHEEHHHCC RFPDYRRVIPKNGDKELISNRESLKQAFSRAAILSNEKFRGVRLILSENLLQITANNPER CCCCHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCH EEAEEYIDVDYHSANLEIGFNVSYVLDVLNTLKSEQVKITFSNSDHSALIESIDSDDSLY HHHHHHCCCEEECCCEEECCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCEE VLMPMRL EEEECCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]