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The map label for this gene is mdeA [H]

Identifier: 119947246

GI number: 119947246

Start: 4457939

End: 4459210

Strand: Reverse

Name: mdeA [H]

Synonym: Ping_3644

Alternate gene names: 119947246

Gene position: 4459210-4457939 (Counterclockwise)

Preceding gene: 119947249

Following gene: 119947245

Centisome position: 97.8

GC content: 44.26

Gene sequence:

>1272_bases
ATGAAATTAGAATCTCTCGCGTTACACTGTGGTTATGAGTCTGAAGCCACTACCAAAGCTGCAGCTGTGCCAATTTATCT
AACCACTTCCTATACTTTTGATGATACCCAGCACGGTGCAGATCTGTTTGATTTAAAGGTTCCCGGTAATATTTATACGC
GTATTATGAATCCTACCACGGATGTACTGGAAAAACGGATCGCAGCGATGGAGGGCGGGATCGGCGCACTGGCCGTGGCA
TCAGGGATGTCAGCAATCACTTATGCGATACAATGTATATGCGAAGTTGGTACTAACATTGTGAGCAGCAGTCAACTCTA
TGGCGGCACTTATAACCTCTTCGCCCACTCATTGCCTAAGCAGGGTATCGAAGCCCGAATGGTCAACTTTGATGATTATC
AGGGCTTTGAGGATGCAATAGATGAAAATACGCGGGCGGTTTTTTGTGAATCAATTGGTAACCCTGCCGGTAATATTGTT
GATCTTGAAAAGTTGGCTGAGATTGCCCATCGTCACGGTGTCCCTCTGATTGTGGATAATACCGTTGCTACACCGGTATT
ATGTCGTCCCTTTGAGTTTGGTGCTGATATTGTGGTGCATTCACTGACGAAATATATCGGCGGTCACGGTACCTGTATTG
GCGGTATTATTGTTGATTCAGGTAAGTTTGACTGGGTCGCAAATAAAAAGCGCTTTGCTATTTTAAATGAACCGGATCCT
TCTTATCATGGTGTTGTTTATACCGAAGCATTAGGTGCTGCAGCTTATATTGGACGTTGCCGTGTTGTACCGTTGCGTAA
TACCGGCGCAGCGATTTCACCAATGAATTCGTTTCAAATTATGCAGGGTTTAGAAACCTTGTGTTTACGCATGGAGCGGC
ACTGTGAAAATGCTGAGAAATTAGCAGCTTACCTCAGTACACATGAAAAAGTTGAGTGGGTTAACTACGCCGCACTTAAA
AGCAGCCCTTATTATTCAATCTGTCAAAAAATATGTGGCGGTAAGGCTTCTGGTATTTTAAGCTTTGGTATTAAAGGCGG
CGCAGTATCAGGCGGTCAATTTATTGATGCGCTGGAGATGATTCTACGCTTAGTTAATATAGGTGATGCTAAGTCGTTAG
CCTGTCACCCGGCTTCAACAACGCATCGCCAGCTGAATCCCGATGAACTAAAAGCGACGGGTGTCAGTGAAGATTTGGTC
CGTATTTCTGTGGGTATTGAAAACATCGATGATATTATTGCCGATGTTGAGCAAGCCTTAGCAAAAGTGTAA

Upstream 100 bases:

>100_bases
GTGATAAATGCATAAAAAGGCTGTTTATTGGACATTAACAGTGCTATTTTTGAACGGTTTTATTAGGGTATACATTCATT
ATTTTTTAATAGGCGTATTT

Downstream 100 bases:

>100_bases
AAACAACTAAGCCGGTATAGCAGCCAATAAATGGTGACAAGCATATCGGCTACGATTTTTAAGAGGCAGTATTATTGTTA
GATGATCCCAAAGTGCTCAG

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKLESLALHCGYESEATTKAAAVPIYLTTSYTFDDTQHGADLFDLKVPGNIYTRIMNPTTDVLEKRIAAMEGGIGALAVA
SGMSAITYAIQCICEVGTNIVSSSQLYGGTYNLFAHSLPKQGIEARMVNFDDYQGFEDAIDENTRAVFCESIGNPAGNIV
DLEKLAEIAHRHGVPLIVDNTVATPVLCRPFEFGADIVVHSLTKYIGGHGTCIGGIIVDSGKFDWVANKKRFAILNEPDP
SYHGVVYTEALGAAAYIGRCRVVPLRNTGAAISPMNSFQIMQGLETLCLRMERHCENAEKLAAYLSTHEKVEWVNYAALK
SSPYYSICQKICGGKASGILSFGIKGGAVSGGQFIDALEMILRLVNIGDAKSLACHPASTTHRQLNPDELKATGVSEDLV
RISVGIENIDDIIADVEQALAKV

Sequences:

>Translated_423_residues
MKLESLALHCGYESEATTKAAAVPIYLTTSYTFDDTQHGADLFDLKVPGNIYTRIMNPTTDVLEKRIAAMEGGIGALAVA
SGMSAITYAIQCICEVGTNIVSSSQLYGGTYNLFAHSLPKQGIEARMVNFDDYQGFEDAIDENTRAVFCESIGNPAGNIV
DLEKLAEIAHRHGVPLIVDNTVATPVLCRPFEFGADIVVHSLTKYIGGHGTCIGGIIVDSGKFDWVANKKRFAILNEPDP
SYHGVVYTEALGAAAYIGRCRVVPLRNTGAAISPMNSFQIMQGLETLCLRMERHCENAEKLAAYLSTHEKVEWVNYAALK
SSPYYSICQKICGGKASGILSFGIKGGAVSGGQFIDALEMILRLVNIGDAKSLACHPASTTHRQLNPDELKATGVSEDLV
RISVGIENIDDIIADVEQALAKV
>Mature_423_residues
MKLESLALHCGYESEATTKAAAVPIYLTTSYTFDDTQHGADLFDLKVPGNIYTRIMNPTTDVLEKRIAAMEGGIGALAVA
SGMSAITYAIQCICEVGTNIVSSSQLYGGTYNLFAHSLPKQGIEARMVNFDDYQGFEDAIDENTRAVFCESIGNPAGNIV
DLEKLAEIAHRHGVPLIVDNTVATPVLCRPFEFGADIVVHSLTKYIGGHGTCIGGIIVDSGKFDWVANKKRFAILNEPDP
SYHGVVYTEALGAAAYIGRCRVVPLRNTGAAISPMNSFQIMQGLETLCLRMERHCENAEKLAAYLSTHEKVEWVNYAALK
SSPYYSICQKICGGKASGILSFGIKGGAVSGGQFIDALEMILRLVNIGDAKSLACHPASTTHRQLNPDELKATGVSEDLV
RISVGIENIDDIIADVEQALAKV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=416, Percent_Identity=31.9711538461538, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI299473759, Length=416, Percent_Identity=29.3269230769231, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI25453487, Length=415, Percent_Identity=28.1927710843373, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=31.1904761904762, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1789383, Length=431, Percent_Identity=24.1299303944316, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17538055, Length=419, Percent_Identity=33.6515513126492, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI71980708, Length=437, Percent_Identity=30.2059496567506, Blast_Score=201, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI17560056, Length=418, Percent_Identity=35.1674641148325, Blast_Score=192, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=49.5391705069124, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319307, Length=431, Percent_Identity=32.0185614849188, Blast_Score=196, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6321254, Length=446, Percent_Identity=26.457399103139, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=304, Percent_Identity=26.9736842105263, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321904, Length=430, Percent_Identity=25.5813953488372, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19922554, Length=421, Percent_Identity=34.2042755344418, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45518; Mature: 45518

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLESLALHCGYESEATTKAAAVPIYLTTSYTFDDTQHGADLFDLKVPGNIYTRIMNPTT
CCCHHEEEECCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCEEEEECCCHHEEEECCCHH
DVLEKRIAAMEGGIGALAVASGMSAITYAIQCICEVGTNIVSSSQLYGGTYNLFAHSLPK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHCCCHHHHHHHHCCC
QGIEARMVNFDDYQGFEDAIDENTRAVFCESIGNPAGNIVDLEKLAEIAHRHGVPLIVDN
CCCCEEEECCCCCCCHHHHHCCCCCEEEEHHCCCCCCCEECHHHHHHHHHHCCCCEEECC
TVATPVLCRPFEFGADIVVHSLTKYIGGHGTCIGGIIVDSGKFDWVANKKRFAILNEPDP
CCCCCEEECCHHCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEECCCEEEEECCCCC
SYHGVVYTEALGAAAYIGRCRVVPLRNTGAAISPMNSFQIMQGLETLCLRMERHCENAEK
CCCCEEEHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHH
LAAYLSTHEKVEWVNYAALKSSPYYSICQKICGGKASGILSFGIKGGAVSGGQFIDALEM
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHH
ILRLVNIGDAKSLACHPASTTHRQLNPDELKATGVSEDLVRISVGIENIDDIIADVEQAL
HHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHH
AKV
HCC
>Mature Secondary Structure
MKLESLALHCGYESEATTKAAAVPIYLTTSYTFDDTQHGADLFDLKVPGNIYTRIMNPTT
CCCHHEEEECCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCEEEEECCCHHEEEECCCHH
DVLEKRIAAMEGGIGALAVASGMSAITYAIQCICEVGTNIVSSSQLYGGTYNLFAHSLPK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHCCCHHHHHHHHCCC
QGIEARMVNFDDYQGFEDAIDENTRAVFCESIGNPAGNIVDLEKLAEIAHRHGVPLIVDN
CCCCEEEECCCCCCCHHHHHCCCCCEEEEHHCCCCCCCEECHHHHHHHHHHCCCCEEECC
TVATPVLCRPFEFGADIVVHSLTKYIGGHGTCIGGIIVDSGKFDWVANKKRFAILNEPDP
CCCCCEEECCHHCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEECCCEEEEECCCCC
SYHGVVYTEALGAAAYIGRCRVVPLRNTGAAISPMNSFQIMQGLETLCLRMERHCENAEK
CCCCEEEHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHH
LAAYLSTHEKVEWVNYAALKSSPYYSICQKICGGKASGILSFGIKGGAVSGGQFIDALEM
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHH
ILRLVNIGDAKSLACHPASTTHRQLNPDELKATGVSEDLVRISVGIENIDDIIADVEQAL
HHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHH
AKV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]