Click here to switch to the map view.

The map label for this gene is hemC

Identifier: 119947244

GI number: 119947244

Start: 4456392

End: 4457324

Strand: Reverse

Name: hemC

Synonym: Ping_3642

Alternate gene names: 119947244

Gene position: 4457324-4456392 (Counterclockwise)

Preceding gene: 119947245

Following gene: 119947243

Centisome position: 97.76

GC content: 42.23

Gene sequence:

>933_bases
ATGACTGCTCAAAAAATTCGTATTGCGACTCGCCATAGCCCTCTGGCTATGTGGCAAGCTAACTTTATTAAATCAGAACT
TTTAAAATATCACCCGAATCTAATCGTTGAACTTTTACCGATGAAAACTAAAGGCGATATAATATTAGATACGCCTTTAG
CTAAAGTGGGCGGAAAAGGTCTTTTTGTTAAAGAGTTAGAAGTTGCCATATTAGAAGGGCGAGCAGATATCGCGGTACAT
TCGATAAAGGATGTGCCTGTTGATTTCCCCGAGGGATTAGGGTTAACAACTATTTGTAAACGAGAAGATCCCCATGATGC
TTTTGTGTCCAATCATTATAAACAGTTAGATGAGCTACCAGAAGGTGCAATAGTGGGGACCTGCAGCCTGCGCCGTCAAT
GTCAGATTCTAGCTTTACGCCCGGACATTATTATTAAAAATCTGCGTGGTAATGTGAATACACGTTTACGTAAGTTAGAT
GAGGGTGAATACGATGCTATTATTTTAGCCGCAGCGGGTTTAATGCGTTTAGAAATGCAACATAGAATCACCTCTTTGAT
CGCACCTGAGGTTAGCCTTCCCGCTGTAGGGCAAGGCGCAGTCGGAATTGAATGCCGTTTAGATGACGAGCAGACTATCA
CCTTATTGAAACCACTTGAAGATCCAGAAACGCGCATAAGAATAACGGCTGAACGAGCAATGAATCTCAAATTGCAGGGC
GGTTGTCAGGTGCCTATTGGGAGCTTTGCCATACTTGAAAATGAACAACTATTTTTGCGCGGTTTAGTTGGCAGTGTAGA
TGGGAAACAGATTATCCGCAAAGAAATAACTGGCCATCAGAATGATGCGCAGCAATTAGGCATTACCCTTGCTGAGCAAT
TATTAGCCTGCGGCGCAGAAGAAATTCTTGCTGAAGTTTATAAAAACCAATGA

Upstream 100 bases:

>100_bases
ATTTTTCCCCCAGATGGGCATTTTTGTGTAAATCCGCATGCTTATTAAGCTTATCTTTGATAGCATCAAAGGATTATTTT
ATTCACGAGAAAATGCATAA

Downstream 100 bases:

>100_bases
AAGAAGCGCGCTTATTAGTGACACGTTTTGCACCTCATGCACAACGCTTAGTGAATTTACTCAATGCGCAGGGGACGGTT
GCCTTTGCGCAACCCTTATT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTAQKIRIATRHSPLAMWQANFIKSELLKYHPNLIVELLPMKTKGDIILDTPLAKVGGKGLFVKELEVAILEGRADIAVH
SIKDVPVDFPEGLGLTTICKREDPHDAFVSNHYKQLDELPEGAIVGTCSLRRQCQILALRPDIIIKNLRGNVNTRLRKLD
EGEYDAIILAAAGLMRLEMQHRITSLIAPEVSLPAVGQGAVGIECRLDDEQTITLLKPLEDPETRIRITAERAMNLKLQG
GCQVPIGSFAILENEQLFLRGLVGSVDGKQIIRKEITGHQNDAQQLGITLAEQLLACGAEEILAEVYKNQ

Sequences:

>Translated_310_residues
MTAQKIRIATRHSPLAMWQANFIKSELLKYHPNLIVELLPMKTKGDIILDTPLAKVGGKGLFVKELEVAILEGRADIAVH
SIKDVPVDFPEGLGLTTICKREDPHDAFVSNHYKQLDELPEGAIVGTCSLRRQCQILALRPDIIIKNLRGNVNTRLRKLD
EGEYDAIILAAAGLMRLEMQHRITSLIAPEVSLPAVGQGAVGIECRLDDEQTITLLKPLEDPETRIRITAERAMNLKLQG
GCQVPIGSFAILENEQLFLRGLVGSVDGKQIIRKEITGHQNDAQQLGITLAEQLLACGAEEILAEVYKNQ
>Mature_309_residues
TAQKIRIATRHSPLAMWQANFIKSELLKYHPNLIVELLPMKTKGDIILDTPLAKVGGKGLFVKELEVAILEGRADIAVHS
IKDVPVDFPEGLGLTTICKREDPHDAFVSNHYKQLDELPEGAIVGTCSLRRQCQILALRPDIIIKNLRGNVNTRLRKLDE
GEYDAIILAAAGLMRLEMQHRITSLIAPEVSLPAVGQGAVGIECRLDDEQTITLLKPLEDPETRIRITAERAMNLKLQGG
CQVPIGSFAILENEQLFLRGLVGSVDGKQIIRKEITGHQNDAQQLGITLAEQLLACGAEEILAEVYKNQ

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=331, Percent_Identity=42.2960725075529, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI66933009, Length=331, Percent_Identity=42.2960725075529, Blast_Score=239, Evalue=2e-63,
Organism=Escherichia coli, GI48994974, Length=308, Percent_Identity=67.8571428571429, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6319996, Length=321, Percent_Identity=38.6292834890966, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI20130425, Length=292, Percent_Identity=39.041095890411, Blast_Score=195, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_PSYIN (A1T0R0)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944924.1
- ProteinModelPortal:   A1T0R0
- SMR:   A1T0R0
- STRING:   A1T0R0
- GeneID:   4624098
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3642
- eggNOG:   COG0181
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- PhylomeDB:   A1T0R0
- BioCyc:   PING357804:PING_3642-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34210; Mature: 34079

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQKIRIATRHSPLAMWQANFIKSELLKYHPNLIVELLPMKTKGDIILDTPLAKVGGKG
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCHHHHCCCC
LFVKELEVAILEGRADIAVHSIKDVPVDFPEGLGLTTICKREDPHDAFVSNHYKQLDELP
EEEHHEEEEEECCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCC
EGAIVGTCSLRRQCQILALRPDIIIKNLRGNVNTRLRKLDEGEYDAIILAAAGLMRLEMQ
CCCEEEEECCCCCEEEEEECCCEEEECCCCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHH
HRITSLIAPEVSLPAVGQGAVGIECRLDDEQTITLLKPLEDPETRIRITAERAMNLKLQG
HHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEEEEHHCEEEEECC
GCQVPIGSFAILENEQLFLRGLVGSVDGKQIIRKEITGHQNDAQQLGITLAEQLLACGAE
CCCCCCCCEEEECCCCEEEHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHH
EILAEVYKNQ
HHHHHHHCCC
>Mature Secondary Structure 
TAQKIRIATRHSPLAMWQANFIKSELLKYHPNLIVELLPMKTKGDIILDTPLAKVGGKG
CCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCHHHHCCCC
LFVKELEVAILEGRADIAVHSIKDVPVDFPEGLGLTTICKREDPHDAFVSNHYKQLDELP
EEEHHEEEEEECCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCC
EGAIVGTCSLRRQCQILALRPDIIIKNLRGNVNTRLRKLDEGEYDAIILAAAGLMRLEMQ
CCCEEEEECCCCCEEEEEECCCEEEECCCCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHH
HRITSLIAPEVSLPAVGQGAVGIECRLDDEQTITLLKPLEDPETRIRITAERAMNLKLQG
HHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEEEEHHCEEEEECC
GCQVPIGSFAILENEQLFLRGLVGSVDGKQIIRKEITGHQNDAQQLGITLAEQLLACGAE
CCCCCCCCEEEECCCCEEEHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHH
EILAEVYKNQ
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA