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The map label for this gene is pldA [H]

Identifier: 119947095

GI number: 119947095

Start: 4323494

End: 4324351

Strand: Direct

Name: pldA [H]

Synonym: Ping_3493

Alternate gene names: 119947095

Gene position: 4323494-4324351 (Clockwise)

Preceding gene: 119947090

Following gene: 119947099

Centisome position: 94.82

GC content: 39.51

Gene sequence:

>858_bases
ATGTATCTATTATTTTTAGTTTTTGTTTTGTTATTTAGCGCGGCCACACGGGCAGAAGAATCTGCCGTTGATCAGCGTAT
CGAGCAGGAGAACAAACTCCAGAAGGACCGCTTTTCTATTATCCCCTATAAACCCAATTACCTTTTGCCTTTTACTTTTA
ATAATAATATTCAATCTTACGATGCTGATAAGGGTGTTGCCGCATCGGACCGATTACAACCCGTTGAGATAAAATTTCAA
CTTAGTTTTAAAACACCTGTCTTAGTGGATATTGCCGACTTACCCCTCACGCTTTATTTTGGTTATACCCAAGTCTCTTT
CTGGCAGGCTTATAATAGCGGTAATTCATCCCCGTTTCGCGAAACTAATTATGAACCGGAGATGTTTCTACGGTGGGTAC
ATGACACAAAACTAGGAGGAGATTGGCATTTTAAATTAGCAACACTGAACTTAGCCCATCAATCCAATGGGAAAACAGAG
CCGGGATCCCGCAGCTGGAATCGTATCGAAAGCAATATTGTCTTGGAAAATGGGAATATCACTCTGGCGTTTAATCCCTG
GTTTCGTTTACCAGAAGATGCAAAAAATGATAATAATCCAGATTTATTGGATTATTATGGCCACGGAAAAATTTATGCTC
TTTATCAAATAAATAAGCATAACTTTTCAATTACCAGCCGCAATAATATTGAAAGTGGCTTTAGCAAAGGGTCACTGGAG
CTCAATTGGAGTGTCCCTCTTCACGGTAGTATTCGCGGTTATTTTCAGGTTTTCTCAGGCTATGGCAACAGCCTGATAGA
GTATAATCAATATACCAATACAGTAGGGTTAGGTATCTCATTAACCGACTGGTTATAG

Upstream 100 bases:

>100_bases
ACCCCAAACAGTTAAACTCGACAATAATTAGAACTTGCATCTATTATGCTTTAAGTTGCACTAATTAGTTCTTTTAGCAT
AATTTATCAAGGAAATGGCT

Downstream 100 bases:

>100_bases
GCCACTGAGCGTATTACACTAACTTCCTGAACAAACAGCAGACGTTTCTATATCCTAGATATACGACGTTACAATAGGGG
TAGGCATCTCATTGACTGAC

Product: phospholipase A(1)

Products: NA

Alternate protein names: Detergent-resistant phospholipase A; DR-phospholipase A; Outer membrane phospholipase A; OM PLA; Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MYLLFLVFVLLFSAATRAEESAVDQRIEQENKLQKDRFSIIPYKPNYLLPFTFNNNIQSYDADKGVAASDRLQPVEIKFQ
LSFKTPVLVDIADLPLTLYFGYTQVSFWQAYNSGNSSPFRETNYEPEMFLRWVHDTKLGGDWHFKLATLNLAHQSNGKTE
PGSRSWNRIESNIVLENGNITLAFNPWFRLPEDAKNDNNPDLLDYYGHGKIYALYQINKHNFSITSRNNIESGFSKGSLE
LNWSVPLHGSIRGYFQVFSGYGNSLIEYNQYTNTVGLGISLTDWL

Sequences:

>Translated_285_residues
MYLLFLVFVLLFSAATRAEESAVDQRIEQENKLQKDRFSIIPYKPNYLLPFTFNNNIQSYDADKGVAASDRLQPVEIKFQ
LSFKTPVLVDIADLPLTLYFGYTQVSFWQAYNSGNSSPFRETNYEPEMFLRWVHDTKLGGDWHFKLATLNLAHQSNGKTE
PGSRSWNRIESNIVLENGNITLAFNPWFRLPEDAKNDNNPDLLDYYGHGKIYALYQINKHNFSITSRNNIESGFSKGSLE
LNWSVPLHGSIRGYFQVFSGYGNSLIEYNQYTNTVGLGISLTDWL
>Mature_285_residues
MYLLFLVFVLLFSAATRAEESAVDQRIEQENKLQKDRFSIIPYKPNYLLPFTFNNNIQSYDADKGVAASDRLQPVEIKFQ
LSFKTPVLVDIADLPLTLYFGYTQVSFWQAYNSGNSSPFRETNYEPEMFLRWVHDTKLGGDWHFKLATLNLAHQSNGKTE
PGSRSWNRIESNIVLENGNITLAFNPWFRLPEDAKNDNNPDLLDYYGHGKIYALYQINKHNFSITSRNNIESGFSKGSLE
LNWSVPLHGSIRGYFQVFSGYGNSLIEYNQYTNTVGLGISLTDWL

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=293, Percent_Identity=35.1535836177474, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 32761; Mature: 32761

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLLFLVFVLLFSAATRAEESAVDQRIEQENKLQKDRFSIIPYKPNYLLPFTFNNNIQSY
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCC
DADKGVAASDRLQPVEIKFQLSFKTPVLVDIADLPLTLYFGYTQVSFWQAYNSGNSSPFR
CCCCCCCCCCCCCEEEEEEEEEECCCEEEEECCCCEEEEECCEEEEEEEHCCCCCCCCCC
ETNYEPEMFLRWVHDTKLGGDWHFKLATLNLAHQSNGKTEPGSRSWNRIESNIVLENGNI
CCCCCHHHEEEEEHHCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCEECCEEEEECCCE
TLAFNPWFRLPEDAKNDNNPDLLDYYGHGKIYALYQINKHNFSITSRNNIESGFSKGSLE
EEEECCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEEECCEEEEEECCCCCCCCCCCCEE
LNWSVPLHGSIRGYFQVFSGYGNSLIEYNQYTNTVGLGISLTDWL
EEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCEEECCEEEECCC
>Mature Secondary Structure
MYLLFLVFVLLFSAATRAEESAVDQRIEQENKLQKDRFSIIPYKPNYLLPFTFNNNIQSY
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCC
DADKGVAASDRLQPVEIKFQLSFKTPVLVDIADLPLTLYFGYTQVSFWQAYNSGNSSPFR
CCCCCCCCCCCCCEEEEEEEEEECCCEEEEECCCCEEEEECCEEEEEEEHCCCCCCCCCC
ETNYEPEMFLRWVHDTKLGGDWHFKLATLNLAHQSNGKTEPGSRSWNRIESNIVLENGNI
CCCCCHHHEEEEEHHCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCEECCEEEEECCCE
TLAFNPWFRLPEDAKNDNNPDLLDYYGHGKIYALYQINKHNFSITSRNNIESGFSKGSLE
EEEECCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEEECCEEEEEECCCCCCCCCCCCEE
LNWSVPLHGSIRGYFQVFSGYGNSLIEYNQYTNTVGLGISLTDWL
EEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCEEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8300539 [H]