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The map label for this gene is glnG [H]

Identifier: 119947090

GI number: 119947090

Start: 4316493

End: 4317893

Strand: Direct

Name: glnG [H]

Synonym: Ping_3488

Alternate gene names: 119947090

Gene position: 4316493-4317893 (Clockwise)

Preceding gene: 119947089

Following gene: 119947095

Centisome position: 94.67

GC content: 42.97

Gene sequence:

>1401_bases
ATGCCTTCAGCTTGGATTGTGGATGATGACCACGCTATTCGTTGGGTTTTAGAACGTACTCTAAAAGCTGAAAAGATTGA
CTATGCCAGTTTCCCCGATGGGGAATGTTTATGGCAACAGTTACAAATAGAGCAGCCTGAGGTCATTATTTCCGACATAC
GGATGCCGGGTATAGATGGACTTGCTTTAATGGAACGTATTCATGGACGCTTTCCATTAATTCCAATTATTATAATGACC
GCACACAGTGATTTAGACAGTGCAGTTAATGCCTACCAAAGTGGCGCCTTTGATTACCTACCAAAACCCTTTGATATTCA
TGAAGCCACGGCTTTAATTCAACGCGCAATTAACCATAGCATTGAATTAAAAAACAAAAAAAAGGTCCCGGAATTACTTA
TTGCTACACCGGAAATTATTGGTGAAGCACCGGCAATGCAGGAGGTGTTCCGCATTATTGGGCGTTTATCTCATTCAAGT
ATGAGTGTATTAATCAATGGTCAATCCGGTACCGGTAAAGAGTTGGTTGCGAAGGCTTTACATAAACACAGCCCACGCAA
AGATAAGACCTTTATCGCCCTGAATATGGCCGCTATTCCCAAAGAGTTGATCGAAGCTGAACTCTTTGGTCATGAAAAAG
GGGCGTTTACCGGTGCGGCTGCAAATCGTCAAGGCCGGTTTGAGCAAGCTAATGGCGGGACTCTTTTTTTAGATGAAATT
GGTGATATGCCATTAGATGTGCAAACCCGTTTATTACGAGTGCTCGCGGATGGTAAATTTTACCGGGTGGGCGGCCATCA
AGGGATCGAAGTCGATGTGCGCATTATTGCAGCTACTCACCAAAATTTAGAAAAAAGTGTGCAATCAGGTAGTTTCCGGG
AAGATCTTTTCCACCGTTTAAATGTCATTCGTATCCAACTCCCCGCTTTAAAAGAGCGTTGTGAAGATATTAGTTTATTG
GCACAACATTTTTTAAATAATATTGGCAAAGAGTTAAATGTCAAAAGCAAGCAATTTACAAAGGAGACAAAAGAGTATTT
AACGCAACTGCCTTGGCCGGGAAACGTACGTCAACTGGAAAACGTTTGCCGCTGGGTGACGGTAATGACCAGCGGGCAGG
AAGTCCATATTTACGATTTACCACCGGAATTAACCGCCCAAGAAACACAAGCTACTGATGATGAAAATCCGCATTGGCAG
AAACAGTTACGCGAATGGACCTTAGCTGAATTAAAACAGGGCAAAGAGAATATACTTGCCAATGCCCTGCCGGAATTCGA
AAAAATAATGTTGCAGGCTGCATTGCAGTACACTCAAGGCAAACGACAGGAAGCCGCAACACTGCTTGGTTGGGGTAGAA
ATACGCTGACCCGTAAATTAAAAGAGTTTGATATCTCCTAA

Upstream 100 bases:

>100_bases
GGAAGGTCATACTGAGTTTCTTATCTATCTGCCTTTAACAATTTAATAAAACATAAAATCAATACTCCCCTATTTAACAC
TTATGAAAAGGAAAAAACAG

Downstream 100 bases:

>100_bases
AGCTGTCGGTTATCAGCTGTCGGCTAACGGCTGCGAGCCTTCGATCTGAAAGCTGAAAGCTGAAAGCTGAAAGCTGAAAG
CTGAAAGCTGAAAGCTGAAA

Product: nitrogen metabolism transcriptional regulator, NtrC, Fis family

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MPSAWIVDDDHAIRWVLERTLKAEKIDYASFPDGECLWQQLQIEQPEVIISDIRMPGIDGLALMERIHGRFPLIPIIIMT
AHSDLDSAVNAYQSGAFDYLPKPFDIHEATALIQRAINHSIELKNKKKVPELLIATPEIIGEAPAMQEVFRIIGRLSHSS
MSVLINGQSGTGKELVAKALHKHSPRKDKTFIALNMAAIPKELIEAELFGHEKGAFTGAAANRQGRFEQANGGTLFLDEI
GDMPLDVQTRLLRVLADGKFYRVGGHQGIEVDVRIIAATHQNLEKSVQSGSFREDLFHRLNVIRIQLPALKERCEDISLL
AQHFLNNIGKELNVKSKQFTKETKEYLTQLPWPGNVRQLENVCRWVTVMTSGQEVHIYDLPPELTAQETQATDDENPHWQ
KQLREWTLAELKQGKENILANALPEFEKIMLQAALQYTQGKRQEAATLLGWGRNTLTRKLKEFDIS

Sequences:

>Translated_466_residues
MPSAWIVDDDHAIRWVLERTLKAEKIDYASFPDGECLWQQLQIEQPEVIISDIRMPGIDGLALMERIHGRFPLIPIIIMT
AHSDLDSAVNAYQSGAFDYLPKPFDIHEATALIQRAINHSIELKNKKKVPELLIATPEIIGEAPAMQEVFRIIGRLSHSS
MSVLINGQSGTGKELVAKALHKHSPRKDKTFIALNMAAIPKELIEAELFGHEKGAFTGAAANRQGRFEQANGGTLFLDEI
GDMPLDVQTRLLRVLADGKFYRVGGHQGIEVDVRIIAATHQNLEKSVQSGSFREDLFHRLNVIRIQLPALKERCEDISLL
AQHFLNNIGKELNVKSKQFTKETKEYLTQLPWPGNVRQLENVCRWVTVMTSGQEVHIYDLPPELTAQETQATDDENPHWQ
KQLREWTLAELKQGKENILANALPEFEKIMLQAALQYTQGKRQEAATLLGWGRNTLTRKLKEFDIS
>Mature_465_residues
PSAWIVDDDHAIRWVLERTLKAEKIDYASFPDGECLWQQLQIEQPEVIISDIRMPGIDGLALMERIHGRFPLIPIIIMTA
HSDLDSAVNAYQSGAFDYLPKPFDIHEATALIQRAINHSIELKNKKKVPELLIATPEIIGEAPAMQEVFRIIGRLSHSSM
SVLINGQSGTGKELVAKALHKHSPRKDKTFIALNMAAIPKELIEAELFGHEKGAFTGAAANRQGRFEQANGGTLFLDEIG
DMPLDVQTRLLRVLADGKFYRVGGHQGIEVDVRIIAATHQNLEKSVQSGSFREDLFHRLNVIRIQLPALKERCEDISLLA
QHFLNNIGKELNVKSKQFTKETKEYLTQLPWPGNVRQLENVCRWVTVMTSGQEVHIYDLPPELTAQETQATDDENPHWQK
QLREWTLAELKQGKENILANALPEFEKIMLQAALQYTQGKRQEAATLLGWGRNTLTRKLKEFDIS

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=463, Percent_Identity=64.5788336933045, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI1788550, Length=464, Percent_Identity=40.301724137931, Blast_Score=340, Evalue=9e-95,
Organism=Escherichia coli, GI1790437, Length=456, Percent_Identity=39.6929824561403, Blast_Score=325, Evalue=5e-90,
Organism=Escherichia coli, GI1788905, Length=465, Percent_Identity=39.1397849462366, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1789233, Length=330, Percent_Identity=44.2424242424242, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI87082117, Length=352, Percent_Identity=39.4886363636364, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1789087, Length=240, Percent_Identity=47.9166666666667, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1786524, Length=337, Percent_Identity=40.9495548961424, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI87082152, Length=242, Percent_Identity=45.0413223140496, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI87081872, Length=336, Percent_Identity=37.797619047619, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1787583, Length=332, Percent_Identity=36.7469879518072, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1789828, Length=229, Percent_Identity=36.2445414847162, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081858, Length=316, Percent_Identity=30.6962025316456, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789809, Length=109, Percent_Identity=33.0275229357798, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52662; Mature: 52531

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSAWIVDDDHAIRWVLERTLKAEKIDYASFPDGECLWQQLQIEQPEVIISDIRMPGIDG
CCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCCCH
LALMERIHGRFPLIPIIIMTAHSDLDSAVNAYQSGAFDYLPKPFDIHEATALIQRAINHS
HHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCC
IELKNKKKVPELLIATPEIIGEAPAMQEVFRIIGRLSHSSMSVLINGQSGTGKELVAKAL
EECCCCCCCCHHHCCCHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
HKHSPRKDKTFIALNMAAIPKELIEAELFGHEKGAFTGAAANRQGRFEQANGGTLFLDEI
HHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCEEEEHHH
GDMPLDVQTRLLRVLADGKFYRVGGHQGIEVDVRIIAATHQNLEKSVQSGSFREDLFHRL
CCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEEHHHHHHHHHHHCCCHHHHHHHHH
NVIRIQLPALKERCEDISLLAQHFLNNIGKELNVKSKQFTKETKEYLTQLPWPGNVRQLE
HEEEEECHHHHHHHHHHHHHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
NVCRWVTVMTSGQEVHIYDLPPELTAQETQATDDENPHWQKQLREWTLAELKQGKENILA
HHHHHHHHEECCCEEEEEECCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHH
NALPEFEKIMLQAALQYTQGKRQEAATLLGWGRNTLTRKLKEFDIS
HCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSAWIVDDDHAIRWVLERTLKAEKIDYASFPDGECLWQQLQIEQPEVIISDIRMPGIDG
CCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCCCH
LALMERIHGRFPLIPIIIMTAHSDLDSAVNAYQSGAFDYLPKPFDIHEATALIQRAINHS
HHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCC
IELKNKKKVPELLIATPEIIGEAPAMQEVFRIIGRLSHSSMSVLINGQSGTGKELVAKAL
EECCCCCCCCHHHCCCHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
HKHSPRKDKTFIALNMAAIPKELIEAELFGHEKGAFTGAAANRQGRFEQANGGTLFLDEI
HHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCEEEEHHH
GDMPLDVQTRLLRVLADGKFYRVGGHQGIEVDVRIIAATHQNLEKSVQSGSFREDLFHRL
CCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEEHHHHHHHHHHHCCCHHHHHHHHH
NVIRIQLPALKERCEDISLLAQHFLNNIGKELNVKSKQFTKETKEYLTQLPWPGNVRQLE
HEEEEECHHHHHHHHHHHHHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
NVCRWVTVMTSGQEVHIYDLPPELTAQETQATDDENPHWQKQLREWTLAELKQGKENILA
HHHHHHHHEECCCEEEEEECCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHH
NALPEFEKIMLQAALQYTQGKRQEAATLLGWGRNTLTRKLKEFDIS
HCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]