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The map label for this gene is ntrB [H]

Identifier: 119947089

GI number: 119947089

Start: 4315368

End: 4316438

Strand: Direct

Name: ntrB [H]

Synonym: Ping_3487

Alternate gene names: 119947089

Gene position: 4315368-4316438 (Clockwise)

Preceding gene: 119947088

Following gene: 119947090

Centisome position: 94.64

GC content: 38.94

Gene sequence:

>1071_bases
ATGTTAAATAATCATTCAAACTCACTTGCAGTTGCCATACTTGAAGAAGCGAATACTGCCTTTGTTATTATTAACGCAAA
ATTAAAACTGATCTATGCAAATCCAGCGAGTGAGGTTTTATTGTCACAAAGCAAACAACGTCTTTACCAGCAAAGTTTGC
AGCAACTGGCAAATCATTATCACTTTGATCCCACCTATATTGAAGCTGTTTTTACCAAACAAAATGCGTTCACTGACAGT
GAAACGACCTTAATGGTTGATGGTCATCCTCTTTTAATGGCATTAAGTGCCACCTATTTAGAGTTTGAAAATCAGAATTA
TGTGATCATTGAGATGCACTGTATTGGACAGCAGCGTAAAATAAGCCAATTTCATTATCAAGATCATCAACAAAAAGTCG
CAAAAGAACTTGTTCGCGGACTTGCTCATGAAATAAAAAATCCACTCGGAGGCCTACGCGGTGCGGCACAATTATTAGAA
AGTGAACTGGCAGATCCTGAACTTAAAGAGTTTACCCAAATAATTATTGAACAAGCGGATCGTCTAAGCGCATTAGTTAA
TCGTTTATTAGGCCCGCAAACCTTGGGAGAGCGCTCCTATCATAATTTACATTCTGTGCTTGAAAAAGTGCGGAAATTAG
TCTGTTTGGGGCTTCCTGAGCATATCCACATCAAAATTGATTATGATCCGAGCATTCCAGATTTTGAAATGCAGCCGGAT
CAACTGCAGCAAGCATTCTTAAATATTCTTCAAAATGCAGTGCAATCACTGAGTACTCAGCCGGAGAAAAACTTAGAAAT
AATTATCAAAACGCGTACTGCCCACCAAGTTATTATTCATGGTGTATTACAACGCTTAGCGGCTGAAATTTCAATTATTG
ATAATGGCCCAGGTGTCCCTGAGCATTTAAAAGATACCTTATTTTACCCTATGGTAACGGGTCGCTGTGATGGTACGGGA
TTGGGGCTATCTATTGCTCAGGAGCTTATTAACCAAGAGAGAGGACGTATCGAATGCGACAGTATGGAAGGTCATACTGA
GTTTCTTATCTATCTGCCTTTAACAATTTAA

Upstream 100 bases:

>100_bases
CAATTATCAGATGAAAGTGGCAAGGTTCTTGCGAGAACACAGGTAGTTACCGTGCATTTACAACGGGTATCCAGCATATC
CGCTCCATAATAAGGCTTAA

Downstream 100 bases:

>100_bases
TAAAACATAAAATCAATACTCCCCTATTTAACACTTATGAAAAGGAAAAAACAGATGCCTTCAGCTTGGATTGTGGATGA
TGACCACGCTATTCGTTGGG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MLNNHSNSLAVAILEEANTAFVIINAKLKLIYANPASEVLLSQSKQRLYQQSLQQLANHYHFDPTYIEAVFTKQNAFTDS
ETTLMVDGHPLLMALSATYLEFENQNYVIIEMHCIGQQRKISQFHYQDHQQKVAKELVRGLAHEIKNPLGGLRGAAQLLE
SELADPELKEFTQIIIEQADRLSALVNRLLGPQTLGERSYHNLHSVLEKVRKLVCLGLPEHIHIKIDYDPSIPDFEMQPD
QLQQAFLNILQNAVQSLSTQPEKNLEIIIKTRTAHQVIIHGVLQRLAAEISIIDNGPGVPEHLKDTLFYPMVTGRCDGTG
LGLSIAQELINQERGRIECDSMEGHTEFLIYLPLTI

Sequences:

>Translated_356_residues
MLNNHSNSLAVAILEEANTAFVIINAKLKLIYANPASEVLLSQSKQRLYQQSLQQLANHYHFDPTYIEAVFTKQNAFTDS
ETTLMVDGHPLLMALSATYLEFENQNYVIIEMHCIGQQRKISQFHYQDHQQKVAKELVRGLAHEIKNPLGGLRGAAQLLE
SELADPELKEFTQIIIEQADRLSALVNRLLGPQTLGERSYHNLHSVLEKVRKLVCLGLPEHIHIKIDYDPSIPDFEMQPD
QLQQAFLNILQNAVQSLSTQPEKNLEIIIKTRTAHQVIIHGVLQRLAAEISIIDNGPGVPEHLKDTLFYPMVTGRCDGTG
LGLSIAQELINQERGRIECDSMEGHTEFLIYLPLTI
>Mature_356_residues
MLNNHSNSLAVAILEEANTAFVIINAKLKLIYANPASEVLLSQSKQRLYQQSLQQLANHYHFDPTYIEAVFTKQNAFTDS
ETTLMVDGHPLLMALSATYLEFENQNYVIIEMHCIGQQRKISQFHYQDHQQKVAKELVRGLAHEIKNPLGGLRGAAQLLE
SELADPELKEFTQIIIEQADRLSALVNRLLGPQTLGERSYHNLHSVLEKVRKLVCLGLPEHIHIKIDYDPSIPDFEMQPD
QLQQAFLNILQNAVQSLSTQPEKNLEIIIKTRTAHQVIIHGVLQRLAAEISIIDNGPGVPEHLKDTLFYPMVTGRCDGTG
LGLSIAQELINQERGRIECDSMEGHTEFLIYLPLTI

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=345, Percent_Identity=48.4057971014493, Blast_Score=327, Evalue=9e-91,
Organism=Escherichia coli, GI1788549, Length=350, Percent_Identity=25.4285714285714, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=29.3103448275862, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789149, Length=229, Percent_Identity=27.9475982532751, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40166; Mature: 40166

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNNHSNSLAVAILEEANTAFVIINAKLKLIYANPASEVLLSQSKQRLYQQSLQQLANHY
CCCCCCCCEEEEEEECCCEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHC
HFDPTYIEAVFTKQNAFTDSETTLMVDGHPLLMALSATYLEFENQNYVIIEMHCIGQQRK
CCCHHHHHHEEECCCCCCCCCCEEEECCCHHHHHHHHHHEEECCCCEEEEEEEECCCHHH
ISQFHYQDHQQKVAKELVRGLAHEIKNPLGGLRGAAQLLESELADPELKEFTQIIIEQAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RLSALVNRLLGPQTLGERSYHNLHSVLEKVRKLVCLGLPEHIHIKIDYDPSIPDFEMQPD
HHHHHHHHHCCCHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHH
QLQQAFLNILQNAVQSLSTQPEKNLEIIIKTRTAHQVIIHGVLQRLAAEISIIDNGPGVP
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHEEEECCCCCCH
EHLKDTLFYPMVTGRCDGTGLGLSIAQELINQERGRIECDSMEGHTEFLIYLPLTI
HHHHHHHHHHCEECCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCEEEEEEEECCC
>Mature Secondary Structure
MLNNHSNSLAVAILEEANTAFVIINAKLKLIYANPASEVLLSQSKQRLYQQSLQQLANHY
CCCCCCCCEEEEEEECCCEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHC
HFDPTYIEAVFTKQNAFTDSETTLMVDGHPLLMALSATYLEFENQNYVIIEMHCIGQQRK
CCCHHHHHHEEECCCCCCCCCCEEEECCCHHHHHHHHHHEEECCCCEEEEEEEECCCHHH
ISQFHYQDHQQKVAKELVRGLAHEIKNPLGGLRGAAQLLESELADPELKEFTQIIIEQAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RLSALVNRLLGPQTLGERSYHNLHSVLEKVRKLVCLGLPEHIHIKIDYDPSIPDFEMQPD
HHHHHHHHHCCCHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHH
QLQQAFLNILQNAVQSLSTQPEKNLEIIIKTRTAHQVIIHGVLQRLAAEISIIDNGPGVP
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHEEEECCCCCCH
EHLKDTLFYPMVTGRCDGTGLGLSIAQELINQERGRIECDSMEGHTEFLIYLPLTI
HHHHHHHHHHCEECCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]