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The map label for this gene is btuB [H]

Identifier: 119946946

GI number: 119946946

Start: 4128734

End: 4130575

Strand: Direct

Name: btuB [H]

Synonym: Ping_3340

Alternate gene names: 119946946

Gene position: 4128734-4130575 (Clockwise)

Preceding gene: 119946945

Following gene: 119946947

Centisome position: 90.55

GC content: 41.42

Gene sequence:

>1842_bases
ATGCCACGCTTATATTACACCCCGATTGCCTTTGCCTTAACGACACTGTTCAGCGCAACAAGTATTGCAGCAGCCCCCCC
CATTTCAGAACAAGATACACGGGATGATATTGTCATTTCTGCCAGTCGCGTAGAAACTAAACGTATTGAAAGCGGCAGCT
CAGTAACAGTGCTTGATGAACAATATATAAAAGAAAATCAAGCGCGCACTGTGGCTGAACTTTTACAGGATGTGCCTGGC
GTGAGTGTTGCCAGTAACGGTGGTTTAGGGCAAGCGACCTCGGTATTTATCCGCGGAGCTAATTCCAATCAAACCTTAGT
GATTATTGACGGTATTGAAGTCAATAACTTAGGGAACTTTGAAGGGGGTTATGATTTTGCATACCTGATGGCAGATAATA
TTGAACGTATAGAAGTCTTAAAAGGCTCGCAAAGCGCACTTTGGGGCAGCGATGCTATGGGTGGCGTGATTAATATCATC
ACTAAAAAAGGCAAAGCAGGATTCCATCCAACGGCTAGTATCGAGGTTGGGGGAAATAACTATCATAAAGAAAATGTCAC
TCTTAGCGCCGCACAGGGTAACAGCCATTATTCGCTATCAGCCAGTAACATGAAAACCGACGGTATTAGTGCAACTGATA
CCGATCCAGATGACGATGGTTACAAAAATCAGAGTGTCAGCCTAAAAGCCGGCCATCAATTTACCGATATTTTCTCAATG
GATACAGTGCTACGTTACAACGATGCAGAAACAGAATATGATTCAGGCTATACCACAAATAGTCAACGTCAGGCAAAATT
AAGCAGCCATCTTAACCTGTTAAATAATCAATGGAAAAATCGTCTTTCAGTCGCTTTTTCTGATTCAAACACCGAAGATT
TTTCCAGCTGGGGCGACAGTAAATATGAAGGGAAAAAAATAAAAACTGATCTGCAGAGTGATTATTATATTAGTGCTATT
AATGGATACACACAGCGCATCTCATTCCTTGCCGAGCACGAAAGTGATAAATATCAAAGTTTGTCAATGACTCAAGACGA
GCGTATCGAAGCATCGGGAGTTGTTTTAGGCTATGGCGTTGACTGGGCAAAAACAATTTTTGTTAACGTAGCTGTTCGTA
GTGACTTTAATAATAAATTTGATGATACCACCACCTACCATATTGATACCTCAGTCTGGGTAAATGATGGTACACGTTTA
CATGCCAGCCATGGTACCGGTCTCAAAAACCCCAATTTAGGACAGTTATATGGTGAGAATGCTAGCTGGGGTTATGTTGG
AAATGCTGATCTAAAACCAGAGAAAAGCCGCAGTTGGGATGCGGGTGTAGAGTATAACTTTGTGGGTACAGATGCTTATA
TCGATCTTACCTATTTTGACTCACTTTATACCGATATGCATACATGGTCTGGTAGTTTCCCTAATTCCACGTATATCAAT
CTTAATAATAAAGCCACTGCACGCGGTATTGAATTTACGGGTAAAGTAAAAGTGAGCAATAAATTACGTGTCAATACAGG
ATATACCTATATGGAGACGAATGACGGTAACGGCAATGAACTGGCTCGTCGACCAAAACATGCAGCCAGTATCAATGCCA
ATTACAAATACACGCCTGAATTAAGTGCCAATATTGGTGCTCGTTATGTTGGTAAACGCCTGGACAGTGATGATTCAACA
CTCTCTAGCTATACGGTTGTCAATATCAGCACTGCATACCAGATTCAGGAACATATCACCCTTTCTGCCCGTATTGAAAA
TGCGCTTGATAAAGACTATCAAGAAGTTTCAGGTTTTAACACTGATCCGCTTACAGCTTATATCGGCTTTAGCTTTAAGT
AA

Upstream 100 bases:

>100_bases
TTTGATGCTCAGCGTATTATATAAAAGGCCACCACCTTACTGTAAAACCCCGAACAACAGACTTTCTTCTTATTTAATAT
AAAAATAACAAGGAAGAAAC

Downstream 100 bases:

>100_bases
AAAAAAGCACTAACGGGAGGGTAAATGAATAATCTAAAACTGATCGTTTTCACCCTTCTTTTAGTGTTTTTTATTACTCT
GATCCACTTTCAAAAAGGGC

Product: TonB-dependent receptor, plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MPRLYYTPIAFALTTLFSATSIAAAPPISEQDTRDDIVISASRVETKRIESGSSVTVLDEQYIKENQARTVAELLQDVPG
VSVASNGGLGQATSVFIRGANSNQTLVIIDGIEVNNLGNFEGGYDFAYLMADNIERIEVLKGSQSALWGSDAMGGVINII
TKKGKAGFHPTASIEVGGNNYHKENVTLSAAQGNSHYSLSASNMKTDGISATDTDPDDDGYKNQSVSLKAGHQFTDIFSM
DTVLRYNDAETEYDSGYTTNSQRQAKLSSHLNLLNNQWKNRLSVAFSDSNTEDFSSWGDSKYEGKKIKTDLQSDYYISAI
NGYTQRISFLAEHESDKYQSLSMTQDERIEASGVVLGYGVDWAKTIFVNVAVRSDFNNKFDDTTTYHIDTSVWVNDGTRL
HASHGTGLKNPNLGQLYGENASWGYVGNADLKPEKSRSWDAGVEYNFVGTDAYIDLTYFDSLYTDMHTWSGSFPNSTYIN
LNNKATARGIEFTGKVKVSNKLRVNTGYTYMETNDGNGNELARRPKHAASINANYKYTPELSANIGARYVGKRLDSDDST
LSSYTVVNISTAYQIQEHITLSARIENALDKDYQEVSGFNTDPLTAYIGFSFK

Sequences:

>Translated_613_residues
MPRLYYTPIAFALTTLFSATSIAAAPPISEQDTRDDIVISASRVETKRIESGSSVTVLDEQYIKENQARTVAELLQDVPG
VSVASNGGLGQATSVFIRGANSNQTLVIIDGIEVNNLGNFEGGYDFAYLMADNIERIEVLKGSQSALWGSDAMGGVINII
TKKGKAGFHPTASIEVGGNNYHKENVTLSAAQGNSHYSLSASNMKTDGISATDTDPDDDGYKNQSVSLKAGHQFTDIFSM
DTVLRYNDAETEYDSGYTTNSQRQAKLSSHLNLLNNQWKNRLSVAFSDSNTEDFSSWGDSKYEGKKIKTDLQSDYYISAI
NGYTQRISFLAEHESDKYQSLSMTQDERIEASGVVLGYGVDWAKTIFVNVAVRSDFNNKFDDTTTYHIDTSVWVNDGTRL
HASHGTGLKNPNLGQLYGENASWGYVGNADLKPEKSRSWDAGVEYNFVGTDAYIDLTYFDSLYTDMHTWSGSFPNSTYIN
LNNKATARGIEFTGKVKVSNKLRVNTGYTYMETNDGNGNELARRPKHAASINANYKYTPELSANIGARYVGKRLDSDDST
LSSYTVVNISTAYQIQEHITLSARIENALDKDYQEVSGFNTDPLTAYIGFSFK
>Mature_612_residues
PRLYYTPIAFALTTLFSATSIAAAPPISEQDTRDDIVISASRVETKRIESGSSVTVLDEQYIKENQARTVAELLQDVPGV
SVASNGGLGQATSVFIRGANSNQTLVIIDGIEVNNLGNFEGGYDFAYLMADNIERIEVLKGSQSALWGSDAMGGVINIIT
KKGKAGFHPTASIEVGGNNYHKENVTLSAAQGNSHYSLSASNMKTDGISATDTDPDDDGYKNQSVSLKAGHQFTDIFSMD
TVLRYNDAETEYDSGYTTNSQRQAKLSSHLNLLNNQWKNRLSVAFSDSNTEDFSSWGDSKYEGKKIKTDLQSDYYISAIN
GYTQRISFLAEHESDKYQSLSMTQDERIEASGVVLGYGVDWAKTIFVNVAVRSDFNNKFDDTTTYHIDTSVWVNDGTRLH
ASHGTGLKNPNLGQLYGENASWGYVGNADLKPEKSRSWDAGVEYNFVGTDAYIDLTYFDSLYTDMHTWSGSFPNSTYINL
NNKATARGIEFTGKVKVSNKLRVNTGYTYMETNDGNGNELARRPKHAASINANYKYTPELSANIGARYVGKRLDSDDSTL
SSYTVVNISTAYQIQEHITLSARIENALDKDYQEVSGFNTDPLTAYIGFSFK

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=648, Percent_Identity=27.9320987654321, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788478, Length=659, Percent_Identity=25.948406676783, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1786798, Length=154, Percent_Identity=33.1168831168831, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 67493; Mature: 67361

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLYYTPIAFALTTLFSATSIAAAPPISEQDTRDDIVISASRVETKRIESGSSVTVLDE
CCCEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCEEEEECH
QYIKENQARTVAELLQDVPGVSVASNGGLGQATSVFIRGANSNQTLVIIDGIEVNNLGNF
HHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCCCEEEEEECEEECCCCCC
EGGYDFAYLMADNIERIEVLKGSQSALWGSDAMGGVINIITKKGKAGFHPTASIEVGGNN
CCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCC
YHKENVTLSAAQGNSHYSLSASNMKTDGISATDTDPDDDGYKNQSVSLKAGHQFTDIFSM
EECCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEHHHHH
DTVLRYNDAETEYDSGYTTNSQRQAKLSSHLNLLNNQWKNRLSVAFSDSNTEDFSSWGDS
CEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCC
KYEGKKIKTDLQSDYYISAINGYTQRISFLAEHESDKYQSLSMTQDERIEASGVVLGYGV
CCCCCCEEECCCCCEEEEEECCHHHHHHHHHHCCCCCCEECCCCCCCCEEECCEEEEECC
DWAKTIFVNVAVRSDFNNKFDDTTTYHIDTSVWVNDGTRLHASHGTGLKNPNLGQLYGEN
CCEEEEEEEEEEECCCCCCCCCCEEEEEEEEEEECCCCEEEECCCCCCCCCCCCCEECCC
ASWGYVGNADLKPEKSRSWDAGVEYNFVGTDAYIDLTYFDSLYTDMHTWSGSFPNSTYIN
CCCCEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEHHHHHHHHHHCCCCCCCCEEEE
LNNKATARGIEFTGKVKVSNKLRVNTGYTYMETNDGNGNELARRPKHAASINANYKYTPE
ECCCCEECCEEEEEEEEECCEEEEECCEEEEECCCCCCHHHHHCCCCCEEECCCEEECCC
LSANIGARYVGKRLDSDDSTLSSYTVVNISTAYQIQEHITLSARIENALDKDYQEVSGFN
CCCCCCHHHHHHHCCCCCCCCCEEEEEEEEEHEEEEHHEEEEEHHHHHHHHHHHHHCCCC
TDPLTAYIGFSFK
CCCEEEEEEEECC
>Mature Secondary Structure 
PRLYYTPIAFALTTLFSATSIAAAPPISEQDTRDDIVISASRVETKRIESGSSVTVLDE
CCEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCEEEEECH
QYIKENQARTVAELLQDVPGVSVASNGGLGQATSVFIRGANSNQTLVIIDGIEVNNLGNF
HHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCCCEEEEEECEEECCCCCC
EGGYDFAYLMADNIERIEVLKGSQSALWGSDAMGGVINIITKKGKAGFHPTASIEVGGNN
CCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCC
YHKENVTLSAAQGNSHYSLSASNMKTDGISATDTDPDDDGYKNQSVSLKAGHQFTDIFSM
EECCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEHHHHH
DTVLRYNDAETEYDSGYTTNSQRQAKLSSHLNLLNNQWKNRLSVAFSDSNTEDFSSWGDS
CEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCC
KYEGKKIKTDLQSDYYISAINGYTQRISFLAEHESDKYQSLSMTQDERIEASGVVLGYGV
CCCCCCEEECCCCCEEEEEECCHHHHHHHHHHCCCCCCEECCCCCCCCEEECCEEEEECC
DWAKTIFVNVAVRSDFNNKFDDTTTYHIDTSVWVNDGTRLHASHGTGLKNPNLGQLYGEN
CCEEEEEEEEEEECCCCCCCCCCEEEEEEEEEEECCCCEEEECCCCCCCCCCCCCEECCC
ASWGYVGNADLKPEKSRSWDAGVEYNFVGTDAYIDLTYFDSLYTDMHTWSGSFPNSTYIN
CCCCEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEHHHHHHHHHHCCCCCCCCEEEE
LNNKATARGIEFTGKVKVSNKLRVNTGYTYMETNDGNGNELARRPKHAASINANYKYTPE
ECCCCEECCEEEEEEEEECCEEEEECCEEEEECCCCCCHHHHHCCCCCEEECCCEEECCC
LSANIGARYVGKRLDSDDSTLSSYTVVNISTAYQIQEHITLSARIENALDKDYQEVSGFN
CCCCCCHHHHHHHCCCCCCCCCEEEEEEEEEHEEEEHHEEEEEHHHHHHHHHHHHHCCCC
TDPLTAYIGFSFK
CCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA