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The map label for this gene is nadE

Identifier: 119946811

GI number: 119946811

Start: 3960442

End: 3961272

Strand: Direct

Name: nadE

Synonym: Ping_3205

Alternate gene names: 119946811

Gene position: 3960442-3961272 (Clockwise)

Preceding gene: 119946809

Following gene: 119946812

Centisome position: 86.86

GC content: 38.75

Gene sequence:

>831_bases
ATGAAACAACAAATATTGCAAGAGATGCGTGTTTTAGTCACTATCAATGAAGAAATAGAAATTAAACGTCGAATCAACTT
TATCAAAAAAAAATTACAGCAAGCTGAATGTTATACTCTGGTTTTGGGTATCAGTGGCGGTGTTGACTCAAGTACCGCCG
GTCGTTTATGTCAACTCGCAGTTGAACAATTAAATCAATCGACAGATACGGACAAATATCAATTTATTGCCGTTCGACTT
CCCTATGCGATTCAGAAAGATGAAGATGAGGCACAATTAGCATTACAATTTATACGACCTTCACATGTTATTACCATTAA
TATAAAAAATGGCGCTGATGGCATACATGAAAGCACCTTAGCGGCACTTCAGACAAGTAGCGTCAATCTTTCTGCCGATA
CCAATATTGACTTTATTAAGGGTAATGTTAAAGCGCGAATGCGAATGATCGCACAATATGAAATTGCAGGGTTAACCGGA
GGGTTAGTTGTCGGAACAGATCACAGTGCAGAAAATATTACCGGCTTTTATACCAAACATGGCGATGGGGCTTGTGATTT
AGCACCCTTATTCGGTTTAAATAAACGTCAGATTCGCGCATTAGCTAAACAGCTTGGCGCACCTGCAATATTAATTGAAA
AAGCACCCACAGCTGACTTGGAAGAAGATAAACCTCAGCTGCAGGATGAACATGCATTAGGGATTACTTATGATCAAATT
GATGATTTCTTAGAAGGTAAAGCTGTCACACAGGAAATTGAAGATAAGTTAATCGCAATTTATCTGCGTACCCAACACAA
GCGTCAAGCTGTTCCAACCATTTATGATTGA

Upstream 100 bases:

>100_bases
ATTAAATGAGCGTCAGCACTATAAAAATCAATTGCTTTTCATCATAATCGAAAACAAAGATTACCAGCTTTTAACTTTTC
CCTAAAATATAGGCTATTTT

Downstream 100 bases:

>100_bases
TCAAATTAATGTTCAACCCTATCTCCAAACTACTTGAAAATGCAGATTTAAGTCGTTTGGGTATAAATGATTAGATTGAA
CATTAACTTTACCTTTTTAT

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKQQILQEMRVLVTINEEIEIKRRINFIKKKLQQAECYTLVLGISGGVDSSTAGRLCQLAVEQLNQSTDTDKYQFIAVRL
PYAIQKDEDEAQLALQFIRPSHVITINIKNGADGIHESTLAALQTSSVNLSADTNIDFIKGNVKARMRMIAQYEIAGLTG
GLVVGTDHSAENITGFYTKHGDGACDLAPLFGLNKRQIRALAKQLGAPAILIEKAPTADLEEDKPQLQDEHALGITYDQI
DDFLEGKAVTQEIEDKLIAIYLRTQHKRQAVPTIYD

Sequences:

>Translated_276_residues
MKQQILQEMRVLVTINEEIEIKRRINFIKKKLQQAECYTLVLGISGGVDSSTAGRLCQLAVEQLNQSTDTDKYQFIAVRL
PYAIQKDEDEAQLALQFIRPSHVITINIKNGADGIHESTLAALQTSSVNLSADTNIDFIKGNVKARMRMIAQYEIAGLTG
GLVVGTDHSAENITGFYTKHGDGACDLAPLFGLNKRQIRALAKQLGAPAILIEKAPTADLEEDKPQLQDEHALGITYDQI
DDFLEGKAVTQEIEDKLIAIYLRTQHKRQAVPTIYD
>Mature_276_residues
MKQQILQEMRVLVTINEEIEIKRRINFIKKKLQQAECYTLVLGISGGVDSSTAGRLCQLAVEQLNQSTDTDKYQFIAVRL
PYAIQKDEDEAQLALQFIRPSHVITINIKNGADGIHESTLAALQTSSVNLSADTNIDFIKGNVKARMRMIAQYEIAGLTG
GLVVGTDHSAENITGFYTKHGDGACDLAPLFGLNKRQIRALAKQLGAPAILIEKAPTADLEEDKPQLQDEHALGITYDQI
DDFLEGKAVTQEIEDKLIAIYLRTQHKRQAVPTIYD

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=277, Percent_Identity=50.5415162454874, Blast_Score=272, Evalue=2e-74,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_PSYIN (A1SZH7)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944491.1
- ProteinModelPortal:   A1SZH7
- SMR:   A1SZH7
- STRING:   A1SZH7
- GeneID:   4624814
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3205
- eggNOG:   COG0171
- HOGENOM:   HBG351567
- OMA:   KVAQATI
- PhylomeDB:   A1SZH7
- ProtClustDB:   PRK00768
- BioCyc:   PING357804:PING_3205-MONOMER
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30605; Mature: 30605

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: ACT_SITE 45-45

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQQILQEMRVLVTINEEIEIKRRINFIKKKLQQAECYTLVLGISGGVDSSTAGRLCQLA
CHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHH
VEQLNQSTDTDKYQFIAVRLPYAIQKDEDEAQLALQFIRPSHVITINIKNGADGIHESTL
HHHHHCCCCCCCEEEEEEEECEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHH
AALQTSSVNLSADTNIDFIKGNVKARMRMIAQYEIAGLTGGLVVGTDHSAENITGFYTKH
HHHHCCCEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEEC
GDGACDLAPLFGLNKRQIRALAKQLGAPAILIEKAPTADLEEDKPQLQDEHALGITYDQI
CCCCCCHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHH
DDFLEGKAVTQEIEDKLIAIYLRTQHKRQAVPTIYD
HHHHCCHHHHHHHHHHEEEEEEECCHHHCCCCCCCC
>Mature Secondary Structure
MKQQILQEMRVLVTINEEIEIKRRINFIKKKLQQAECYTLVLGISGGVDSSTAGRLCQLA
CHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHH
VEQLNQSTDTDKYQFIAVRLPYAIQKDEDEAQLALQFIRPSHVITINIKNGADGIHESTL
HHHHHCCCCCCCEEEEEEEECEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHH
AALQTSSVNLSADTNIDFIKGNVKARMRMIAQYEIAGLTGGLVVGTDHSAENITGFYTKH
HHHHCCCEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEEC
GDGACDLAPLFGLNKRQIRALAKQLGAPAILIEKAPTADLEEDKPQLQDEHALGITYDQI
CCCCCCHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHH
DDFLEGKAVTQEIEDKLIAIYLRTQHKRQAVPTIYD
HHHHCCHHHHHHHHHHEEEEEEECCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA