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The map label for this gene is pilT [H]

Identifier: 119946660

GI number: 119946660

Start: 3738841

End: 3739875

Strand: Reverse

Name: pilT [H]

Synonym: Ping_3039

Alternate gene names: 119946660

Gene position: 3739875-3738841 (Counterclockwise)

Preceding gene: 119946671

Following gene: 119946659

Centisome position: 82.02

GC content: 42.03

Gene sequence:

>1035_bases
ATGGATATAACAGAATTATTGGCTTTCAGTGTAAAGCATAATGCGTCAGATCTACACCTTTCTTCAGGAGTTCCTCCAAT
GATTAGGGTCGATGGGGAGGTGCGTAAATTAAATGTCCCTGCTTTAGATCCTAAAGAGGCACAGACTCTTATTTACGACA
TTATGAATGATAAACAGCGTAAAGATTTTGAAGAGGATTTAGAAACCGACTTTTCCTTTGAAATTCCAGGCTTAGCGCGT
TTCCGTGTTAATGCTTTTAACCAGAACCGTGGTGTATCGGCTGTCTTTCGTACTATCCCAAGCAAAGTGTTAACATTAGA
TGAACTGGGCGCACCTGAAATATTTAAAACAATATCAGAATACCCTCGCGGTTTAGTCTTAGTAACGGGGCCAACGGGCT
CGGGTAAGTCAACCACCTTAGCAGCAATGATTGACTATATTAATGAAAGTCGTCATGAGCACATTTTAACCATCGAAGAT
CCTGTCGAATTTGTCCATGAAACAAAAAAATCGCTGATCAACCAGCGTGAAGTTTATAGACACACCTTAAGTTTTCAGCG
CGCACTAAAAAGTGCATTGCGGGAAGATCCCGATATTATTTTAGTCGGAGAACTGCGTGATTTAGAAACCATTCGTTTAG
CAATCACAGCAGCGGAAACGGGTCACTTGGTCTTTGCTACCCTGCATACCACTTCTGCGGCTAAAACGATTAACCGTATT
ATTGATGTCTTTCCTGCGGGAGAGAAAAGCATGGTGCGCTCAATGTTATCTGAATCATTACGTGCCGTTATTTCGCAAGC
TTTACTTAAAAAACCGGGTGGCGGGCGAGTTGCTGCGCATGAAATTATGTTGGGCATTCCCGCAATTCGGAATTTGATTC
GTGAAGATAAAGTTGCGCAAATGTACTCAGTAATTCAAACCGGAATGGTACATGGCATGCAAACACTAGATCAAAGTTTA
CGTGATTTAGTTGGCCAGGGTCTTATTGCACACGCTGATGCTACGAAAAAAGCGGTCGATCCAAAAACATTCTAA

Upstream 100 bases:

>100_bases
GATCAGCATTCCGGCCCGCTCGTTGAGCCGCCTGAATGATTGTTTGTTCTACATTTTTAATTTTTTTAGATATATTAGTC
ATAAATAAGGTTTTAATCAC

Downstream 100 bases:

>100_bases
GGGCAATTTATTATGTTAGAAAAGTTATTAATTAAGGTCGATGAATCAAAAGCATCTGATTTATATATTTCAGTTGGTTT
GCCGGCAACAATGAAATTAA

Product: type II secretion system protein (twitching motility protein) (PilT)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MDITELLAFSVKHNASDLHLSSGVPPMIRVDGEVRKLNVPALDPKEAQTLIYDIMNDKQRKDFEEDLETDFSFEIPGLAR
FRVNAFNQNRGVSAVFRTIPSKVLTLDELGAPEIFKTISEYPRGLVLVTGPTGSGKSTTLAAMIDYINESRHEHILTIED
PVEFVHETKKSLINQREVYRHTLSFQRALKSALREDPDIILVGELRDLETIRLAITAAETGHLVFATLHTTSAAKTINRI
IDVFPAGEKSMVRSMLSESLRAVISQALLKKPGGGRVAAHEIMLGIPAIRNLIREDKVAQMYSVIQTGMVHGMQTLDQSL
RDLVGQGLIAHADATKKAVDPKTF

Sequences:

>Translated_344_residues
MDITELLAFSVKHNASDLHLSSGVPPMIRVDGEVRKLNVPALDPKEAQTLIYDIMNDKQRKDFEEDLETDFSFEIPGLAR
FRVNAFNQNRGVSAVFRTIPSKVLTLDELGAPEIFKTISEYPRGLVLVTGPTGSGKSTTLAAMIDYINESRHEHILTIED
PVEFVHETKKSLINQREVYRHTLSFQRALKSALREDPDIILVGELRDLETIRLAITAAETGHLVFATLHTTSAAKTINRI
IDVFPAGEKSMVRSMLSESLRAVISQALLKKPGGGRVAAHEIMLGIPAIRNLIREDKVAQMYSVIQTGMVHGMQTLDQSL
RDLVGQGLIAHADATKKAVDPKTF
>Mature_344_residues
MDITELLAFSVKHNASDLHLSSGVPPMIRVDGEVRKLNVPALDPKEAQTLIYDIMNDKQRKDFEEDLETDFSFEIPGLAR
FRVNAFNQNRGVSAVFRTIPSKVLTLDELGAPEIFKTISEYPRGLVLVTGPTGSGKSTTLAAMIDYINESRHEHILTIED
PVEFVHETKKSLINQREVYRHTLSFQRALKSALREDPDIILVGELRDLETIRLAITAAETGHLVFATLHTTSAAKTINRI
IDVFPAGEKSMVRSMLSESLRAVISQALLKKPGGGRVAAHEIMLGIPAIRNLIREDKVAQMYSVIQTGMVHGMQTLDQSL
RDLVGQGLIAHADATKKAVDPKTF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=327, Percent_Identity=48.3180428134557, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI1789723, Length=348, Percent_Identity=33.0459770114943, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1786296, Length=280, Percent_Identity=33.5714285714286, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38114; Mature: 38114

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITELLAFSVKHNASDLHLSSGVPPMIRVDGEVRKLNVPALDPKEAQTLIYDIMNDKQR
CCHHHHHHHHHCCCCCCEEECCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHHCCHHH
KDFEEDLETDFSFEIPGLARFRVNAFNQNRGVSAVFRTIPSKVLTLDELGAPEIFKTISE
CCHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEHHHCCCHHHHHHHHH
YPRGLVLVTGPTGSGKSTTLAAMIDYINESRHEHILTIEDPVEFVHETKKSLINQREVYR
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
HTLSFQRALKSALREDPDIILVGELRDLETIRLAITAAETGHLVFATLHTTSAAKTINRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCCEEEEEEEHHHHHHHHHHH
IDVFPAGEKSMVRSMLSESLRAVISQALLKKPGGGRVAAHEIMLGIPAIRNLIREDKVAQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
MYSVIQTGMVHGMQTLDQSLRDLVGQGLIAHADATKKAVDPKTF
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHCCCCCCC
>Mature Secondary Structure
MDITELLAFSVKHNASDLHLSSGVPPMIRVDGEVRKLNVPALDPKEAQTLIYDIMNDKQR
CCHHHHHHHHHCCCCCCEEECCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHHCCHHH
KDFEEDLETDFSFEIPGLARFRVNAFNQNRGVSAVFRTIPSKVLTLDELGAPEIFKTISE
CCHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEHHHCCCHHHHHHHHH
YPRGLVLVTGPTGSGKSTTLAAMIDYINESRHEHILTIEDPVEFVHETKKSLINQREVYR
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
HTLSFQRALKSALREDPDIILVGELRDLETIRLAITAAETGHLVFATLHTTSAAKTINRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCCEEEEEEEHHHHHHHHHHH
IDVFPAGEKSMVRSMLSESLRAVISQALLKKPGGGRVAAHEIMLGIPAIRNLIREDKVAQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
MYSVIQTGMVHGMQTLDQSLRDLVGQGLIAHADATKKAVDPKTF
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]