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The map label for this gene is pilU [H]

Identifier: 119946659

GI number: 119946659

Start: 3737713

End: 3738828

Strand: Reverse

Name: pilU [H]

Synonym: Ping_3038

Alternate gene names: 119946659

Gene position: 3738828-3737713 (Counterclockwise)

Preceding gene: 119946660

Following gene: 119946658

Centisome position: 82.0

GC content: 38.8

Gene sequence:

>1116_bases
ATGTTAGAAAAGTTATTAATTAAGGTCGATGAATCAAAAGCATCTGATTTATATATTTCAGTTGGTTTGCCGGCAACAAT
GAAATTAAATGGAACATTAAAGGCTATTAATGAACATATACTGACCAGCGAAGAAGTTTATGATCTCTTAAAAGAGGTCA
TGGATGAAAAACATTTTAATGAATTTATGACTATTAAAGAATCCAATTTTGCGCACCATCCCAACGATGGTAAGCGTTTT
AGGATCAGTGCTTTTATGCAAAAAGGGCAACCTGCTATGGTGCTCCGCCGTATAGAAACTAAAATACCTGAATTTGCTGA
GATAAATTTACCGGAACGATTAAAAGATGTCTGTCTGGCAAAAGAGGGCTTAATTTTATTTGTAGGTGCAACGGGTGTCG
GTAAATCAACGACACAAGCAGCAATGATTGGTTTTCGAAATCAAAATTCATGCGATCATATCCTGACCATTGAAGATCCG
ATTGAGTTTATTCACGAACATGGAAAATCTGTGATCACCCAACGCGAAATTGGTGTTGATACTGGTTCATTTGATGAGGC
GCTGAAAAATTCATTACGCCAAGCGGCTGATGTTATTGTCGTCGGTGAAATACGAACACGACAAACCATGGAATTCGTTC
TGTCATTTGCTGAGACCGGGCATTTATGTATGGCGACTATGCATGCTAAAAATGCGAATCAGGCTTTAGATCGTATTCTA
CATTTAGTGCCGCAGGAAAATCATCGCCAGTTTCTATTTGATCTGTCGGTCAATTTACGTGCAATAGTTGCCCAGCAGTT
AATTCCAACAACAGATGGACAAGGGCGGCGAGCTGCATTTGAAATTTTATATAATACACCGACCATGGCTGAAGCTATTC
GTACTGGCGAGCTGCATACCCTTAAACAGATTATGAAATCATCAGGACAACATGGTATGCAAACCTTTGACCAAGCGCTG
TTTGATTTATATGAAAGCGGCATTATCGGTTATACCGAAGCATTGGCCTATTCTGATTCAGCAAATGATGTACGCTTAAT
GATTAAGCTTAAATCAACTGAAAGCCGAAAAAGTTTAGACAGTGGCAAGCTTTCTGATGTTACAGTCGACTTTTAA

Upstream 100 bases:

>100_bases
AGATCAAAGTTTACGTGATTTAGTTGGCCAGGGTCTTATTGCACACGCTGATGCTACGAAAAAAGCGGTCGATCCAAAAA
CATTCTAAGGGCAATTTATT

Downstream 100 bases:

>100_bases
TCTCTACCCCTTGTGATTATTTTAATAAAAAAGCGGCATGAGTCGCTTTTTAATAACCCGTTATTAATAGGAAGTCGAAT
GTTAGTTGTACTCTCTCCGG

Product: type II secretion system protein (twitching motility protein) (PilU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MLEKLLIKVDESKASDLYISVGLPATMKLNGTLKAINEHILTSEEVYDLLKEVMDEKHFNEFMTIKESNFAHHPNDGKRF
RISAFMQKGQPAMVLRRIETKIPEFAEINLPERLKDVCLAKEGLILFVGATGVGKSTTQAAMIGFRNQNSCDHILTIEDP
IEFIHEHGKSVITQREIGVDTGSFDEALKNSLRQAADVIVVGEIRTRQTMEFVLSFAETGHLCMATMHAKNANQALDRIL
HLVPQENHRQFLFDLSVNLRAIVAQQLIPTTDGQGRRAAFEILYNTPTMAEAIRTGELHTLKQIMKSSGQHGMQTFDQAL
FDLYESGIIGYTEALAYSDSANDVRLMIKLKSTESRKSLDSGKLSDVTVDF

Sequences:

>Translated_371_residues
MLEKLLIKVDESKASDLYISVGLPATMKLNGTLKAINEHILTSEEVYDLLKEVMDEKHFNEFMTIKESNFAHHPNDGKRF
RISAFMQKGQPAMVLRRIETKIPEFAEINLPERLKDVCLAKEGLILFVGATGVGKSTTQAAMIGFRNQNSCDHILTIEDP
IEFIHEHGKSVITQREIGVDTGSFDEALKNSLRQAADVIVVGEIRTRQTMEFVLSFAETGHLCMATMHAKNANQALDRIL
HLVPQENHRQFLFDLSVNLRAIVAQQLIPTTDGQGRRAAFEILYNTPTMAEAIRTGELHTLKQIMKSSGQHGMQTFDQAL
FDLYESGIIGYTEALAYSDSANDVRLMIKLKSTESRKSLDSGKLSDVTVDF
>Mature_371_residues
MLEKLLIKVDESKASDLYISVGLPATMKLNGTLKAINEHILTSEEVYDLLKEVMDEKHFNEFMTIKESNFAHHPNDGKRF
RISAFMQKGQPAMVLRRIETKIPEFAEINLPERLKDVCLAKEGLILFVGATGVGKSTTQAAMIGFRNQNSCDHILTIEDP
IEFIHEHGKSVITQREIGVDTGSFDEALKNSLRQAADVIVVGEIRTRQTMEFVLSFAETGHLCMATMHAKNANQALDRIL
HLVPQENHRQFLFDLSVNLRAIVAQQLIPTTDGQGRRAAFEILYNTPTMAEAIRTGELHTLKQIMKSSGQHGMQTFDQAL
FDLYESGIIGYTEALAYSDSANDVRLMIKLKSTESRKSLDSGKLSDVTVDF

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=306, Percent_Identity=36.6013071895425, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1789723, Length=156, Percent_Identity=36.5384615384615, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786296, Length=230, Percent_Identity=28.695652173913, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41558; Mature: 41558

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKLLIKVDESKASDLYISVGLPATMKLNGTLKAINEHILTSEEVYDLLKEVMDEKHFN
CCCCEEEEECCCCCCCEEEEECCCEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EFMTIKESNFAHHPNDGKRFRISAFMQKGQPAMVLRRIETKIPEFAEINLPERLKDVCLA
HHEEEECCCCCCCCCCCCEEEEEHHHHCCCHHHHHHHHHHCCCCCEECCCHHHHHHHHHC
KEGLILFVGATGVGKSTTQAAMIGFRNQNSCDHILTIEDPIEFIHEHGKSVITQREIGVD
CCCEEEEEECCCCCCCHHHHHHEECCCCCCCCEEEEEHHHHHHHHHCCHHHHHHHHCCCC
TGSFDEALKNSLRQAADVIVVGEIRTRQTMEFVLSFAETGHLCMATMHAKNANQALDRIL
CCCHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHCCCHHHHHHHHH
HLVPQENHRQFLFDLSVNLRAIVAQQLIPTTDGQGRRAAFEILYNTPTMAEAIRTGELHT
HHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHCCCHHH
LKQIMKSSGQHGMQTFDQALFDLYESGIIGYTEALAYSDSANDVRLMIKLKSTESRKSLD
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHEECCCCCCEEEEEEEECCCCHHCCC
SGKLSDVTVDF
CCCCCCEEECC
>Mature Secondary Structure
MLEKLLIKVDESKASDLYISVGLPATMKLNGTLKAINEHILTSEEVYDLLKEVMDEKHFN
CCCCEEEEECCCCCCCEEEEECCCEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EFMTIKESNFAHHPNDGKRFRISAFMQKGQPAMVLRRIETKIPEFAEINLPERLKDVCLA
HHEEEECCCCCCCCCCCCEEEEEHHHHCCCHHHHHHHHHHCCCCCEECCCHHHHHHHHHC
KEGLILFVGATGVGKSTTQAAMIGFRNQNSCDHILTIEDPIEFIHEHGKSVITQREIGVD
CCCEEEEEECCCCCCCHHHHHHEECCCCCCCCEEEEEHHHHHHHHHCCHHHHHHHHCCCC
TGSFDEALKNSLRQAADVIVVGEIRTRQTMEFVLSFAETGHLCMATMHAKNANQALDRIL
CCCHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHCCCHHHHHHHHH
HLVPQENHRQFLFDLSVNLRAIVAQQLIPTTDGQGRRAAFEILYNTPTMAEAIRTGELHT
HHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHCCCHHH
LKQIMKSSGQHGMQTFDQALFDLYESGIIGYTEALAYSDSANDVRLMIKLKSTESRKSLD
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHEECCCCCCEEEEEEEECCCCHHCCC
SGKLSDVTVDF
CCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]