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The map label for this gene is ydiB [C]

Identifier: 119946623

GI number: 119946623

Start: 3699704

End: 3700522

Strand: Reverse

Name: ydiB [C]

Synonym: Ping_3002

Alternate gene names: 119946623

Gene position: 3700522-3699704 (Counterclockwise)

Preceding gene: 119946626

Following gene: 119946621

Centisome position: 81.16

GC content: 44.93

Gene sequence:

>819_bases
ATGACCCAAGTTATCAATAAAGAGACTAAAGTATGTATGTCTCTTGCCGCAAGGCCTAGTAATTTCGGTACCCGTTTCCA
CAATTTTTTATATCAGGCGCTGGATCTGGATTATTTGTACAAAGCTTTTTCAACCACGGATTTACCCGGTGCCATTGGGG
GTGTAAGGGCCCTTGGTATTCGCGGCTGCGCGATATCTATGCCGTTTAAAGAGGAGGTTATCGAATTGGTAGATGAATTG
GACCCATCGGCAAAAGCAATCAACTCAGTGAATACGATCGTCAATGAAAAGGGTTACCTTAAAGCCTATAATACCGACTA
TATAGCTATCGCTACCTTGTTATCACAATACCAGCTGAACAAAGATCTGGTCTTTGCATTAAAAGGCAGTGGCGGAATGG
CAAAAGCGGTTGCCTGTGCGTTAAAAGATGCGGGTTTCCCTCACGGTTACATTGTCGCCAGAAGCGAAAAAAGGGGTAAG
AAATTAGCGCAATTAAGTGGTTATCAGTGGCTCCCTGATACGCAAGGACTGGAAGCCGATCTACTGATTAACGTAACACC
AATTGGCATGTCCGGAGGCCCGGATGAAAATGCCCTCTCTTTTACGGAAAGTGAAGTGGATACTGCAGAGATTGTTTTTG
ATGTCGTTGCACTGCCCGCCATTACCCCATTGATAAGTTATGCCAAATCACAGGGAAAAACAGTGATCACCGGCAGTGAA
GTGTTTGCATTGCAGGCAGTAGAGCAATTTGTCCTATATACCGGTATTCGCCCTAGTGATGCCTTGTTTGAGGAAGCAGC
AACATATTCTCGAAGCTGA

Upstream 100 bases:

>100_bases
CAGTTTCCCAGCAAGTTTTATCTTGGCATTATAGTGCTTATTTTTCGACCACCAATGAGTGTTATAAACGATTGAACATA
TTATTTAAGGAGTCCCCCTC

Downstream 100 bases:

>100_bases
TAAAAATGGCGTCTTCTTTTGATCGTTGGCTGACGCTCCTTAAAGAGGCGTTGCACAAAAGCCAACAACGATAAAAAACA
AGGCGACTTTATTGACGGTT

Product: shikimate 5-dehydrogenase

Products: 3-dehydroshikimate; NADPH; H+

Alternate protein names: SDH-L [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTQVINKETKVCMSLAARPSNFGTRFHNFLYQALDLDYLYKAFSTTDLPGAIGGVRALGIRGCAISMPFKEEVIELVDEL
DPSAKAINSVNTIVNEKGYLKAYNTDYIAIATLLSQYQLNKDLVFALKGSGGMAKAVACALKDAGFPHGYIVARSEKRGK
KLAQLSGYQWLPDTQGLEADLLINVTPIGMSGGPDENALSFTESEVDTAEIVFDVVALPAITPLISYAKSQGKTVITGSE
VFALQAVEQFVLYTGIRPSDALFEEAATYSRS

Sequences:

>Translated_272_residues
MTQVINKETKVCMSLAARPSNFGTRFHNFLYQALDLDYLYKAFSTTDLPGAIGGVRALGIRGCAISMPFKEEVIELVDEL
DPSAKAINSVNTIVNEKGYLKAYNTDYIAIATLLSQYQLNKDLVFALKGSGGMAKAVACALKDAGFPHGYIVARSEKRGK
KLAQLSGYQWLPDTQGLEADLLINVTPIGMSGGPDENALSFTESEVDTAEIVFDVVALPAITPLISYAKSQGKTVITGSE
VFALQAVEQFVLYTGIRPSDALFEEAATYSRS
>Mature_271_residues
TQVINKETKVCMSLAARPSNFGTRFHNFLYQALDLDYLYKAFSTTDLPGAIGGVRALGIRGCAISMPFKEEVIELVDELD
PSAKAINSVNTIVNEKGYLKAYNTDYIAIATLLSQYQLNKDLVFALKGSGGMAKAVACALKDAGFPHGYIVARSEKRGKK
LAQLSGYQWLPDTQGLEADLLINVTPIGMSGGPDENALSFTESEVDTAEIVFDVVALPAITPLISYAKSQGKTVITGSEV
FALQAVEQFVLYTGIRPSDALFEEAATYSRS

Specific function: The physiological substrate is not known. Has much lower activity towards shikimate than AroE [H]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787983, Length=240, Percent_Identity=32.5, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789675, Length=242, Percent_Identity=26.4462809917355, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320332, Length=264, Percent_Identity=25, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR006151 [H]

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N [H]

EC number: 1.1.1.25

Molecular weight: Translated: 29387; Mature: 29256

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVINKETKVCMSLAARPSNFGTRFHNFLYQALDLDYLYKAFSTTDLPGAIGGVRALGI
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
RGCAISMPFKEEVIELVDELDPSAKAINSVNTIVNEKGYLKAYNTDYIAIATLLSQYQLN
CCEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCC
KDLVFALKGSGGMAKAVACALKDAGFPHGYIVARSEKRGKKLAQLSGYQWLPDTQGLEAD
CCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCEE
LLINVTPIGMSGGPDENALSFTESEVDTAEIVFDVVALPAITPLISYAKSQGKTVITGSE
EEEEEEECCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCH
VFALQAVEQFVLYTGIRPSDALFEEAATYSRS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TQVINKETKVCMSLAARPSNFGTRFHNFLYQALDLDYLYKAFSTTDLPGAIGGVRALGI
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
RGCAISMPFKEEVIELVDELDPSAKAINSVNTIVNEKGYLKAYNTDYIAIATLLSQYQLN
CCEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCC
KDLVFALKGSGGMAKAVACALKDAGFPHGYIVARSEKRGKKLAQLSGYQWLPDTQGLEAD
CCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCEE
LLINVTPIGMSGGPDENALSFTESEVDTAEIVFDVVALPAITPLISYAKSQGKTVITGSE
EEEEEEECCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCH
VFALQAVEQFVLYTGIRPSDALFEEAATYSRS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: shikimate; NADP+

Specific reaction: shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]