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The map label for this gene is tilS [H]

Identifier: 119946581

GI number: 119946581

Start: 3649465

End: 3650847

Strand: Reverse

Name: tilS [H]

Synonym: Ping_2960

Alternate gene names: 119946581

Gene position: 3650847-3649465 (Counterclockwise)

Preceding gene: 119946582

Following gene: 119946579

Centisome position: 80.07

GC content: 42.95

Gene sequence:

>1383_bases
ATGAATAAAGCTGATTTACTTAATGCATTTCAGGCGCAATTAAACTTAATCCTTGCCCCGAAAATAAGCTTTGATCAAAA
TTTTCATATCGCGCTTAGCGGGGGATTGGATTCAGTGGTGCTATTGCATCTATTTACACGATTGCGCGCATTTGATAAAA
AATATTCTCTTTGTGCCCACCATATAAACCATGGTTTAAGTGATAATGCTGCCCATTGGCAACTGTTTTGCGAAAATTTA
TGTGCTTCATTAGCGGTTGATTTTTGCTGCTCGCCGGTGATTTTAGAGAAAAAAAATCGTACTAGCCTGGAAGCCCTGGC
AAGGGAAAAGCGCTATGCTTGTTTAACCGGAAACCTGCTCGATAATAGCTATTTAATCACCGCCCATCATCAGGATGATC
AACTGGAAACTGTTTTGCTTGCTTTAAAACGCGGCTCGGGCAGTACAGGTTTACAAGGCATTCAAAAAATGCAAAAACTG
CAAAAAGGGCATTTAATTCGGCCTTTATTAAACTTTTCGCGTCAGCAGTTAGCGGCCTATGCAGAGCTTTTTCAGTTAGA
TTGGGTCGAGGATGAAAGTAATCAGGATCAGGATTTTGATCGTAATTTTATTCGCCAGACTATTACGCCCTTATTAAAAG
AACGCTGGCCGGGCATGGGCAAAGCCGTAGCGCGTAGCGCTAATATTTGTCAGGAACAGCAGCAGCTCTTAGATGAAATT
GCACTGGCAGATTTTAATCAACTGGTTGAATTTTCACTCAATCAACAAGTATTACCCATTGAAGACTTAGCGGCACTCAG
CATTGGCCGGCGCAATAATCTATTACGCTTTTGGTTTAAAAAAAACCAACTAGACTACCCATCGGCAAAACAATTACAGG
CACTTTGGACTGATGTCGCGTTAGCCATTATTGACGCACAACCCTTGCTGCAATTTAAAGGTTACTCAGTTCGCCGCTAC
CGTAACTATCTTTATCTGGTTAATGATAATAAGCTCGCCGCCAGTATTGCCCAGAAAATTGAGTGGCAGGGGCAGGATAA
AATTGTTTTACAGGGTGGATTAGTTGAATTGTCCTTTAGTATTGAGGAAGACGCTAAGGCCGCGGGAAATTTACTTAACT
TGGATGGTAATGCAAAAGTTGAAATCTGTTTCCGCTGCCAACTACCGGCCAATTTAGCCTGCCAGCCCTTGGGGAGAAAG
GGTTCCCGCAGTATCAAAAAACTTCTGCACGAATACCATGTTGCTCCCTGGTTACGCGATTTAGTGCCCTTTATTCTGCT
CGATGGCAAGCTTAAAATGGCGGTCGGATTATGGTCTTGCCAGTCTGAAAAAACAGCCGGCAAGCCACGTTATTTAAACT
GTCAGCTGAAGGCTATCGGCTGA

Upstream 100 bases:

>100_bases
GATGATCTTTTATTTTCATTATCCAGACTGCTTGGGCCTAAAAATGTGACTTTGCATTTTGATCATGTTAACTAGTTAAA
CAGACCCTAAAGGATCATAA

Downstream 100 bases:

>100_bases
CAGCTTTCAGATCGAAGCCCTCAAGCCTTAAAGCACGCAGCTCGAAACTCGAAGCTCGCAGCTATCTGTTCTTCAAATAA
GGTGCCAGTTGTTTAGCCAT

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNKADLLNAFQAQLNLILAPKISFDQNFHIALSGGLDSVVLLHLFTRLRAFDKKYSLCAHHINHGLSDNAAHWQLFCENL
CASLAVDFCCSPVILEKKNRTSLEALAREKRYACLTGNLLDNSYLITAHHQDDQLETVLLALKRGSGSTGLQGIQKMQKL
QKGHLIRPLLNFSRQQLAAYAELFQLDWVEDESNQDQDFDRNFIRQTITPLLKERWPGMGKAVARSANICQEQQQLLDEI
ALADFNQLVEFSLNQQVLPIEDLAALSIGRRNNLLRFWFKKNQLDYPSAKQLQALWTDVALAIIDAQPLLQFKGYSVRRY
RNYLYLVNDNKLAASIAQKIEWQGQDKIVLQGGLVELSFSIEEDAKAAGNLLNLDGNAKVEICFRCQLPANLACQPLGRK
GSRSIKKLLHEYHVAPWLRDLVPFILLDGKLKMAVGLWSCQSEKTAGKPRYLNCQLKAIG

Sequences:

>Translated_460_residues
MNKADLLNAFQAQLNLILAPKISFDQNFHIALSGGLDSVVLLHLFTRLRAFDKKYSLCAHHINHGLSDNAAHWQLFCENL
CASLAVDFCCSPVILEKKNRTSLEALAREKRYACLTGNLLDNSYLITAHHQDDQLETVLLALKRGSGSTGLQGIQKMQKL
QKGHLIRPLLNFSRQQLAAYAELFQLDWVEDESNQDQDFDRNFIRQTITPLLKERWPGMGKAVARSANICQEQQQLLDEI
ALADFNQLVEFSLNQQVLPIEDLAALSIGRRNNLLRFWFKKNQLDYPSAKQLQALWTDVALAIIDAQPLLQFKGYSVRRY
RNYLYLVNDNKLAASIAQKIEWQGQDKIVLQGGLVELSFSIEEDAKAAGNLLNLDGNAKVEICFRCQLPANLACQPLGRK
GSRSIKKLLHEYHVAPWLRDLVPFILLDGKLKMAVGLWSCQSEKTAGKPRYLNCQLKAIG
>Mature_460_residues
MNKADLLNAFQAQLNLILAPKISFDQNFHIALSGGLDSVVLLHLFTRLRAFDKKYSLCAHHINHGLSDNAAHWQLFCENL
CASLAVDFCCSPVILEKKNRTSLEALAREKRYACLTGNLLDNSYLITAHHQDDQLETVLLALKRGSGSTGLQGIQKMQKL
QKGHLIRPLLNFSRQQLAAYAELFQLDWVEDESNQDQDFDRNFIRQTITPLLKERWPGMGKAVARSANICQEQQQLLDEI
ALADFNQLVEFSLNQQVLPIEDLAALSIGRRNNLLRFWFKKNQLDYPSAKQLQALWTDVALAIIDAQPLLQFKGYSVRRY
RNYLYLVNDNKLAASIAQKIEWQGQDKIVLQGGLVELSFSIEEDAKAAGNLLNLDGNAKVEICFRCQLPANLACQPLGRK
GSRSIKKLLHEYHVAPWLRDLVPFILLDGKLKMAVGLWSCQSEKTAGKPRYLNCQLKAIG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=436, Percent_Identity=35.5504587155963, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 52011; Mature: 52011

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKADLLNAFQAQLNLILAPKISFDQNFHIALSGGLDSVVLLHLFTRLRAFDKKYSLCAH
CCHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
HINHGLSDNAAHWQLFCENLCASLAVDFCCSPVILEKKNRTSLEALAREKRYACLTGNLL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHCCCEEEECCCC
DNSYLITAHHQDDQLETVLLALKRGSGSTGLQGIQKMQKLQKGHLIRPLLNFSRQQLAAY
CCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AELFQLDWVEDESNQDQDFDRNFIRQTITPLLKERWPGMGKAVARSANICQEQQQLLDEI
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ALADFNQLVEFSLNQQVLPIEDLAALSIGRRNNLLRFWFKKNQLDYPSAKQLQALWTDVA
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
LAIIDAQPLLQFKGYSVRRYRNYLYLVNDNKLAASIAQKIEWQGQDKIVLQGGLVELSFS
HHHCCCCHHHHHCCCEEEEECCEEEEEECCHHHHHHHHHHCCCCCCEEEEECCEEEEEEC
IEEDAKAAGNLLNLDGNAKVEICFRCQLPANLACQPLGRKGSRSIKKLLHEYHVAPWLRD
CCHHHHHCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LVPFILLDGKLKMAVGLWSCQSEKTAGKPRYLNCQLKAIG
HHHHHEECCCEEEEEEEEECCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MNKADLLNAFQAQLNLILAPKISFDQNFHIALSGGLDSVVLLHLFTRLRAFDKKYSLCAH
CCHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
HINHGLSDNAAHWQLFCENLCASLAVDFCCSPVILEKKNRTSLEALAREKRYACLTGNLL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHCCCEEEECCCC
DNSYLITAHHQDDQLETVLLALKRGSGSTGLQGIQKMQKLQKGHLIRPLLNFSRQQLAAY
CCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AELFQLDWVEDESNQDQDFDRNFIRQTITPLLKERWPGMGKAVARSANICQEQQQLLDEI
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ALADFNQLVEFSLNQQVLPIEDLAALSIGRRNNLLRFWFKKNQLDYPSAKQLQALWTDVA
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
LAIIDAQPLLQFKGYSVRRYRNYLYLVNDNKLAASIAQKIEWQGQDKIVLQGGLVELSFS
HHHCCCCHHHHHCCCEEEEECCEEEEEECCHHHHHHHHHHCCCCCCEEEEECCEEEEEEC
IEEDAKAAGNLLNLDGNAKVEICFRCQLPANLACQPLGRKGSRSIKKLLHEYHVAPWLRD
CCHHHHHCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LVPFILLDGKLKMAVGLWSCQSEKTAGKPRYLNCQLKAIG
HHHHHEECCCEEEEEEEEECCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]